Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Vaccination of Shrimp (Penaeus chinensis) against White Spot Syndrome Virus (WSSV)

  • Ha, Yu-Mi (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Gong, Soo-Jung (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Nguyen, Thi-Hoai (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Ra, Chae-Hun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim, Ki-Hong (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2008.05.31
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Abstract

Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.

Keywords

References

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