• Title/Summary/Keyword: miR-195

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MicroRNA super-resolution imaging in blood for Alzheimer's disease

  • Mirae Lee;Jiwon Woo;Sang Tae Kim;Minho Moon;Sang Yun Kim;Hanna Cho;Sujin Kim;Han-Kyeol Kim;Jeong-Yoon Park
    • BMB Reports
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    • v.56 no.3
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    • pp.190-195
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    • 2023
  • We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer's disease (AD). Here, we report a single-molecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases.

Optimization of Extraction Conditions for the Antibacterial Activity by Clove against Streptococcus mutans Using Evolutionary Operation-Factorial Design Technique

  • Choi, Ung-Kyu;Kim, Mi-Hyang;Kwon, Dae-Jun;Kwon, O-Jun;Lee, Nan-Hee
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.899-903
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    • 2008
  • This study was conducted in order to elucidate the optimum conditions for the extraction of clove that can be used to elicit antibacterial activity against Streptococcus mutans using the evolutionary operation (EVOP)-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature of $80^{\circ}C$ ($r=0.7983^{**}$) and in a longer extraction time of 26 hr ($r=0.6867^*$). Antibacterial activity was not effected by differentiation of ethanol concentration in the extraction solvent (r=-0.0683). The maximum antibacterial activity of clove against S. mutans as determined by the EVOP-factorial design technique was obtained at an extraction temperature of $80^{\circ}C$, an extraction time of 26 hr and a 50% ethanol concentration. Furthermore, the population of S. mutans decreased from an initial concentration of 6.850 to 4.195 log CFU/mL in the third set that is more than 2.6 log cycles by EVOP-factorial design technique.

Anti-inflammatory Effect of Isaria sinclairii Glycosaminoglycan in an Adjuvant-treated Arthritis Rat Model

  • Ahn, Mi Young;Jee, Sang Duck;Hwang, Jae Sam;Yun, Eun Young;Ahn, Kwang Seok;Kim, Yeong Shik
    • Toxicological Research
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    • v.29 no.3
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    • pp.195-201
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    • 2013
  • The anti-inflammatory effects of glycosaminoglycan (GAG) derived from Isaria sinclairii (IS) and of IS extracts were investigated in a complete Freund's adjuvant (CFA)-treated chronic arthritis rat model. Groups of rats were treated orally with 30 mg/kg one of the following: [1] saline control, extracts of [2] water-IS, [3] methanol-IS, [4] butanol-IS, [5] ethyl acetate-IS, or [6] Indomethacin(R) as the positive control for a period of two weeks. The anti-paw edema effects of the individual extracts were in the following order: water-IS ex. > methanol ex. > butanol ex. > ethyl acetate ex. The water/methanol extract from I. sinclairii remarkably inhibited UV-mediated upregulation of NF-${\kappa}B$ activity in transfected HaCaT cells. GAG as a water-soluble alcohol precipitated fraction also produced a noticeable anti-edema effect. This GAG also inhibited the pro-inflammatory cytokine levels of prostaglandin $E_2$-stimulated lipopolysaccharide in LAW 264.7 cells, cytokine TNF-${\alpha}$ production in splenocytes, and atherogenesis cytokine levels of vascular endothelial growth factor (VEGF) production in HUVEC cells in a dose-dependent manner. In the histological analysis, the LV dorsal root ganglion, including the articular cartilage, and linked to the paw-treated IS GAG, was repaired against CFA-induced cartilage destruction. Combined treatment with Indomethacin(R) (5 mg/kg) and IS GAG (10 mg/kg) also more effectively inhibited CFA-induced paw edema at 3 hr, 24 hr, and 48 hr to levels comparable to the anti-inflammatory drug, indomethacin. Thus, the IS GAG described here holds great promise as an anti-inflammatory drug in the future.

Effect of Major Satisfaction and Academic Engagement on Confidence in Performing Core Nursing Skills of Nursing Students (간호대학생의 전공만족도, 학습열의가 핵심간호술 수행자신감에 미치는 효과)

  • Bo-Ra Kim;Mi-Ra Jung
    • Journal of Industrial Convergence
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    • v.21 no.10
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    • pp.151-158
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    • 2023
  • The purpose of this study was to investigate the effect of major satisfaction, academic engagement of confidence in performing core nursing skills in nursing students. The subjects were nursing students residing in J provincial and 112 questionnaires were analyzed. There were significant differences in confidence in performing core nursing skills with age(F=5.52, p<.001), Images about the nurse profession(F=10.2, p<.001). There was positive correlation between confidence in performing core nursing skills and major satisfaction (r=.563, <.001). The factors affecting confidence in performing core nursing skills of the major satisfaction(𝛽=.338 P<.001), academic engagement(𝛽=.195, p<.027), explaining 35% of the variance. Therefore, based on the results of this study, it will be an important basic data for the development of education to improve the core nursing skills of nursing university students.

The Relationships in Emotional Intelligence, Job Satisfaction, and Quality of Nursing Service in Hospital Nurses (병원 간호사의 감성지능, 직무만족, 간호서비스 질의 관계)

  • Jang, Ra-jin;Kang, Young-Sil;Kim, Yu-mi
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.326-337
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    • 2016
  • This study was conducted to identify the relationships between emotional intelligence, job satisfaction, and quality of nursing service for hospital nurses, and to serve as a basis for improvement of nursing service and nursing resources management. All data were collected and conducted with the cooperation of the nursing service department in a tertiary general hospital and two general hospitals located in G province. Questionnaires were completed by 195 nurses who consented to participation in the study from 7 to 28 April 2014. Data were analyzed using SPSS 18.0. A highly positive relationship (r=0.624, p<0.001, r=0.612, p<0.001) was observed between emotional intelligence and quality of nursing service in the superior general hospitals and the general hospitals. Based on this study, quality of nursing service could be improved by a positive relationship between emotional intelligence and job satisfaction in the superior general hospitals and general hospitals (r=0.430, p<0.001). Therefore, it is important to provide a good occupational environment and develop educational programs to enhance the emotional intelligence of nurses, and a positive measure focused on less experienced and young nurses to improve emotional intelligence.

A Correlational Study among Perceived Stress, Anger Expression, and Depression in Cancer Patients* (암 환자의 지각된 스트레스, 분노표현방식과 우울간의 관계)

  • Lee Pyong-Sook;Sohn Jung-Nam;Lee, Yong-Mi;Park Eun-Young;Park Ji-Sun
    • Journal of Korean Academy of Nursing
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    • v.35 no.1
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    • pp.195-205
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    • 2005
  • Purpose: This study was to identify the relationship between perceived stress, anger expression, and level of depression in cancer patients. Method: A cross-sectional descriptive study design was used. Data was collected by uestionnaires from 185 in- and out-patients who were diagnosed with cancer at 3 university hospitals and the National Cancer Center using Spielberger et al.’s Anger Expression Scale, Cohen, Kamarch & Mermelstein's Perceived Stress, and Derogatise's SCL-90. The data was analyzed using descriptive statistics, Pearson correlation coefficient, and stepwise multiple regression with SAS/PC. Result: The perceived stress in cancer patients indicated a significant positive correlation to anger-in(r=.288, p=.000), anger-out(r=.232, p=.001), and depression(r=.68, p=.000), but no significant correlation to anger-control. The anger-in of cancer patients showed a significant positive relationship to anger-out(r=.53, p=.000), and depression(r=.383, p=.000), but no significant correlation to anger-control. Anger-out showed a significantly negative correlation to anger-control (r=-.248, p=.001) and a positive correlation to depression (r=.240, p=.001). The most significant predictor which influenced depression in cancer patients was perceived stress, followed by anger-in and hobby, and these factors explained their depression with a variance of 54%. Conclusion: These results suggested that cancer patients with a high degree of perceived stress are likely to be high in anger-out and anger-in. Perceived stress and anger-in are major factors which affect depression in cancer patients.

Analysis of Domestic and Overseas Disaster Research Trends - Focusing on ICT (국내·외 재난 연구 동향 분석 - ICT 중심으로)

  • Kim, Gwan-Jun;Lee, Gang-Jun;Back, Sung-Won;Sim, Young-Mi;Jeong, Sang
    • Proceedings of the Korean Society of Disaster Information Conference
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    • 2023.11a
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    • pp.195-197
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    • 2023
  • 본 연구는 재난에서 사용되고 있는 정보통신기술( ICT) 연구 국내논문 100개 및 해외논문 100개를 수집한 후, R 프로그램을 이용한 텍스트 마이닝 분석을 통해 국내 재난관리 네 가지 단계에 따른 ICT 기술 관련 연구 동향을 파악하고, 해외 재난관리의 ICT 기술 연구 동향을 분석 활용하여 우리나라 재난에서의 ICT 기술 활용방안을 새롭게 구성해 제언하는 것이다.

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High School Girl's Level of Stress, Somatization, Anger and Adjustment to School according to the Types of Housing (거주형태에 따른 여고생들의 스트레스, 신체화, 분노 및 학교적응)

  • Park, Yeon-Suk;Kim, Jong-Lim;Lee, Seon-Mi
    • The Korean Journal of Health Service Management
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    • v.6 no.3
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    • pp.183-195
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    • 2012
  • The purpose of this study was to examine the level of stress, somatization, anger, adjustment to school according to the types of housing. This study employed a descriptive design. Data was collected from 552 students in a girls high school in gongju using structured instruments. Not to be influenced by the tension of new school year or the stress by taking tests, the researcher did the survey after students finishing midterm test of the first semester for two days from July 14 to 15. The results are as follows. There was no difference in the level of stress, anger, adjustment to school between the students living in a dormitory and the students not living in a dormitory. However there was remarkable difference in somatization. There was positive correlation between somatization and stress(r=.194, p=.011), between anger and stress(r=.463, p<.001), in contrast there was a negative correlation between adjustment to school and stress(r=-.174, p<.001) of students living in a dormitory. On the other hand, there was negative correlation stress(r=-.187, p<.001), somatization(r=-.252, p<.001), anger(r=-.230, p<.001) with adjustment to school of students not living in a dormitory. In the sub-factors of somatization, students who live in a dormitory have many kinds of somatizations of digestive or respiratory organs. A Health promotion program should be designed for girls high school students living in a dormitory, based on the level of somatization of digestive or respiratory organs.

Perceptions of Patient Safety Culture, Safety Care Knowledge and Activity among Nurses at an Orthopedic Hospital (중소 정형외과병원 간호사의 환자안전문화인식, 환자안전지식과 환자안전간호수행)

  • Kim, Mi Young;Eun, Young
    • Journal of muscle and joint health
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    • v.24 no.1
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    • pp.14-23
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    • 2017
  • Purpose: The purpose of this study was to identify the factors affecting the perceptions of patient safety culture, safety care knowledge, and safety care activity among nurses at orthopedic hospitals. Methods: Data were collected during Feb. 16 and Feb. 26, 2017, from 195 nurses of 9 small to medium sized orthopedic hospitals. Questionnaires about patient safety culture, safety care knowledge and activity were used. Data were analyzed by descriptive statistics, t-test, ANOVA, $Scheff\acute{e}$ test, Pearson's correlation coefficient, and stepwise multiple regression. Results: The safety care activity was positively correlated with perceptions of patient safety culture (r=.50, p<.001) and knowledge (r=.48, p<.001). Factors that had influence on the safety care activity were the patient safety culture (${\beta}=.30$, p<.001), age (${\beta}=.27$, p<.001), and knowledge of the safety activity (${\beta}=.21$, p=.004). The patient safety care activity was explained 36.6% by those factors. Conclusion: To enhance the patient safety care activity, it should be provided the environment and open communication for the perceptions of patient safety culture and the in service education program for safety care knowledge.

A Study on the Factors of the Older Adults' Memory Performance (노인의 기억수행에 영향을 미치는 요인에 관한 연구)

  • Min, Hye-Sook
    • The Korean Journal of Rehabilitation Nursing
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    • v.3 no.2
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    • pp.181-195
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    • 2000
  • The purpose of this study is to find out the effects of personal characteristics, social support, depression, and metamemory on the older adults' memory performances. The subjects of the study consisted of 95 older adults over the age of 60 who are living in Busan. Some data were collected by means of the interview method, using questionnaires for metamemory (MIA questionnaire by Dixon, et al., 1988), and depression(GDS by Yesavage and Sheikl, 1986), social resoueces(Duke university, 1978), family support(Hyun-Sook Kang, 1985). The other data were collected by the testing method on the memory performance such as the immeadiate word recall task, the delayed word recall task, the word recognition task(Elderly Verbal Learning Test by Kyung Mi Choi, 1998), and the face recognition task(Face Recognition Task tool developed by Hye-Sook Min). The results of this study were as follows; 1. The level of metamemory is 3.4 points in the 5 point scale, the grades of the task and the achievement are relatively high and the grades of the change, the control, and the anxiety are relatively low in the sub-concepts. 2. Metamemory have significant relation with age(r=-.44), educational attainment(r=.46), depression(r=-.58), family support(r=.20), social resources(r=.20). 3. The significant variables to predict older adults' metamemory are educational attainment(22%), sex(11%), age (8.3%), depression(4.7%), and illness state(3%). 4. The strong variables to predict memory performances are metamemory, age, depression, social resources, educational attainment, illness state, and limitation of daily living activity related to illness. In conclusion, the enhancement strategies of metamemory and the social support and the prevention or reduction of depression are necessary to increase older adults' memory performances. Ultimately in this respect nurses' roles are very important in developing and performing some intervention programs for old adults' memory improvement, which have significant meanings in the field of nursing science.

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