• The Korean Journal of Physiology and Pharmacology
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• v.16 no.2
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• pp.79-89
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• 2012

We examine whether Phellodendron amurense (PA) and its major alkaloid compound, berberine (BER), improved memory defects caused by administering scopolamine in rats. Effects of PA and BER on the acetylcholinergic system and pro-inflammatory cytokines in the hippocampus were also investigated. Male rats were administered daily doses for 14 days of PA (100 and 200 mg/kg, i.p.) and BER (20 mg/kg, i.p.) 30 min before scopolamine injection (2 mg/kg, i.p.). Daily administration of PA and BER improved memory impairment as measured by the passive avoidance test and reduced the escape latency for finding the platform in the Morris water maze test. Administration of PA and BER significantly alleviated memory-associated decreases in cholinergic immunoreactivity and restored brain-derived neurotrophic factor and cAMP-response element-binding protein mRNA expression in the hippocampus. PA and BER also decreased significantly the expression of proinflammatory cytokines such as interleukin-$1{\beta}$, tumor necrosis factor-${\alpha}$ and cyclooxygenase-2 mRNA in the hippocampus. These results demonstrated that PA and BER had significant neuroprotective effects against neuronal impairment and memory dysfunction caused by scopolamine in rats. These results suggest that PA and BER may be useful as therapeutic agents for improving cognitive functioning by stimulating cholinergic enzyme activity and alleviating inflammatory responses.

• Journal of Korean Medical classics
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• v.20 no.4
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• pp.17-35
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• 2007

Objectives : To consider same or not the positions of acupoints on the meridian in the old acupuncture books. Methods: On the basis of old acu-books, We count the number of acupoints that are on pulse. Thru the three books-"Yijongkumkam(醫宗金鑑)","Chimkuhak 1st(鍼灸學-上)", Kyunghyulhakchongseo(經穴學叢書)"-We Compared the way of positioning of acupoints, also did on the location of acupoints, vein, vascular system, around the acupoints, based on the anatomical structures(esp. artery) Results : On the basis of old acu-books, the number of acupoints(only in the twelve meridian) on the pulse is 35, and the number of points not in the old books but on the pulse is 6, sum is 41 points. Comparion from Sambukuhu consultation method(三部九候診法) in "Somun(素問)", Some points, Taeyang(太陽, not in the twelve meridian), Yimun(耳門) and Koryo(Yimun and Koryo are in) are on pulse. Like Chonbu(天府) and Hyupbaek(俠白), We can feel the pulse on ulna side of biceps muscle, noton radial side. Shikdu(食竇) was described as on pulse in the "Chimkushimbopyokyul(鍼灸心法要訣)", but we could hardly feel it. Conclusions : It'll be right to add the Yimun(耳門) and Koryo to the points on pulse, of course two points are not in the twelve meridian. We must take the points Chonbu(天府) and Hyupbaek(俠白), on ulna side of biceps muscle. Shikdu(食竇) must be excepted, because we cannot feel the pulse in consultation.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.24 no.4
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• pp.313-318
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• 2006

Recently, the single plane coordinate system which has one origin is required to create and manage continuous geographic framework data of entire Korean peninsula. For this, UTM-K (Univercial Transverse Mercator-K) was established in 2005. In this paper, the level of distortion was analyzed with respect to the central meridian and scale factor of UTM-K. The latitude and longitude values of the center point of 1/25000 scale digital maps were used for calculating the scale factor which was created by projection and the scale factor was used for index that presents the degree of distortion. As a result, accumulated distortion of scale factor by UTM-K map projection showed about $23.744{\times}10^{-2}$. On the other hand, the minimum distortion which was about $5.1435{\times}10^{-2}$ occurred when central meridian is $127^{\circ}\;42'$ and scale factor of central meridian was 0.99994 respectively.

• Korean Journal of Acupuncture
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• v.26 no.3
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• pp.27-42
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• 2009

Objectives : The vestibular system detects head movement and serve to regulate and maintain the equilibrium and orientation of the body. It is known that the vestibular imbalance leads to vestibular symptoms such as nausea, vomiting, vertigo and postural disturbance. The objectives of the present study were to examine a modification of the dynamic activities of medial vestibular nucleus (MVN) neurons following electroacupuncture (EA) of GB43 (Hyepgye). Methods : In Sprague-Dawley rats weighing $250{\sim}300g$, dynamic responses induced by sinusoidal whole body rotation about vertical axis at 0.2 Hz were observed in MVN of rats during EA of GB43 (Hyepgye) with 0.2 ms, 40 Hz and $600{\pm}200{\mu}A$. Also, expression of cFos protein was observed 2 hours after EA for 30 mins. Results : In dynamic response of vestibular neuron, the excitatory or inhibitory responses of gain were predominant in the ipsilateral MVN neurons during EA but not predominant in the contralateral MVN. Most neurons showing decreased gain were classified to inhibitory responses of spontaneous firing discharge during EA and ones showing increased gain were classified to excitatory response of spontaneous firing discharge during EA. Also, EA of the left GB43 (Hyepgye) for 30 mins produced the expression of cFos protein in MVN, inferior olive (IO) and solitary tract nuclei (SOL). Spatial expressions of cFos protein were predominant in the contralateral MVN, ipsilateral IO and bilateral SOL. Conclusion : These results suggest that the excitability of MVN neurons was influenced by EA of GB43 (Hyepgye) and EA may be related to the convergence on MVN.

• Korean Journal of Acupuncture
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• v.22 no.4
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• pp.161-168
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• 2005

Objective : <千金方(Qianjinfang)> which documents a great amount of prescription of Acupuncture and moxibustion takes an important role during the development of acupuncture and moxibustion. Thus, it have given rise us to the research-situation of Tang Dynasty China of previous period. This study was to outline the rules of choosing acupoints as well as the prescriptions of contemporary times in reference with <千金方(Qianjinfang)>, and to discuss the conception of acupuncture and moxibustion in <千金方(Qianjinfang)>. Method : Based on <千金方(Qianjinfang)>, the parameters were categorized to a certain scope, syndromes in different type were classified. Also numbers of acupoints, route of meridians locations, utilities of special acupoints even prescription methods were statistically analyzed in reference with <千金方(Qianjinfang)>. Results & Conclusion : Acupuncture prescription in <千金方(Qianjinfang)> was mostly presented by single acupoint and this was basic prescription of its rule of choosing acupoints. Choosing acupoint for the majority of various diseases started from choosing meridians with the disorder, but no rules for choosing acupoints was clear. There was basically various methods in choosing acupoints, but little were used by Biao-li meridian choosing method (表裏經配穴). In the high frequency of use, wu-shu acupoints was higher used than other specific acupoints (特定穴). While wu-shu acupoints treated the major parts of the diseases, shu-mo acupoints were strictly used on system. Consequently, The theoretical basis originating the choosing rules of the acupoints and prescriptions was shown in <手金方(Qianjinfang)> as representative clinical reference, through which it was valuable to analyze the prescription rule and specificity of the acupoints.

• Korean Journal of Acupuncture
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• v.24 no.1
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• pp.161-170
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• 2007

Objectives : In this study, we determinated protective effects of Vitis labrusca Root herbal-acupuncture solution (VLHA) against atherosclerosis and cardiovascular disease induced by oxidative damage and cytochrome P450 (CYP) induction. Methods : Antioxidant capacity of VLHA were evaluated by the ${\beta}-carotene/linoleic$ acid emulsion system and FeCl2-ascorbic acid stimulted lipid peroxidation in rat liver homogenate, respectively. Scavenging effects of VLHA on ONOO- and NO were measured by DHR 123, DAF-2 assay. Furthermore, we measured inhibitory activity of VLHA on CYP 2E1 in rat liver microsome using by Aniline assay. Results : VLHA exhibited potent inhibition rate(74%) on FeCl2-ascorbic acid induced lipid peroxidation, and revealed a strong scavenging effects on ONOO- and NO. In addition, VLHA showed inhibitory effect on CYP 2E1. Conclusions : The present study concludes that our results suggest that VLHA could be used as a good source in the prevention of atherosclerosis and alcoholic liver injury.

• Proceedings of the IEEK Conference
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• 2002.06e
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• pp.117-120
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• 2002

This paper is to develop the sensing system for opening-force measurement such as O-Ring muscular meridian. We designed to overcome the functional limit that the conventional force-sensor can measure just the closing-force. Therefore, the new sensor can meet a variety of application as well as O-Ring test. The structure of the new sensor is an actuator-type system using an electromagnet. That is made up of mechanical system, electromagnet, current transformer and computer interface circuit. Driving software and user interface program of the new sensor system also is explained in this paper.

• Journal of Korean Ophthalmic Optics Society
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• v.8 no.1
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• pp.11-15
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• 2003

Purpose : The purpose of this study is to investigate the corneal topography changes as a result of near task in myopes and emmetropes. Methods : Thirty university students, aged from 18 to 24 years old, were randomly selected. They were divided into two refractive groups of fifteen each : myopia and emmetropia. The corneal topography of each subject was measured with the Eye-Sys Videokeratography system. Measurements were taken : before and after 30 minutes of near task (copy N10 text at 20 cm working distance). Both simulated keratometry and semi-meridian keratometric map program were used in the data analysis. Results : Our results reveal no significant changes in both simulated keratometry and semi-meridian presentation as a result of near task for both myopes and emmetropes, except a significant change (p<0.05) found at the flattest meridian of the central (3 mm) portion of the corneal topography after near task for emmetropes only. Conclusions : We conclude that the corneal topography, does not change significantly as a result of near task in both myopes and emmetropes.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.614-620
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• 2014

Kainic acid (KA) is a excitatory agonist causing epileptic seizure and excitotoxicity in the hippocampus. Gastrodia Elata (GE) is known to have anti-convulsant and anti-oxidant effects. This study was investigated a possible role of GE in suppressing epileptic seizure using KA-induced epilepsy mouse model. Eight-week-old male C57BL/6 mice were administrated GE (50 or 500 mg/kg) once a day for 5 days, and then injected KA (30 mg/kg) intraperitoneally. Behavioral changes in mice by KA were evaluated for 90 minutes immediately after the KA administration. Six hours after the KA administration, their brains were harvested and the expressions of glutamate decarboxylase 67 (GAD-67) and K+-Cl- cotransporter 2 (KCC2) in the hippocampus of the mice were measured by immunohistochemistry.GE delayed the onset of epileptic seizure after KA administration, suppressed the severity of the seizure and decreased the number of severe seizures dose dependently. Moreover, GAD-67 and KCC2 expressions in the cornu ammonis (CA) 1 and CA3 of 500 mg/kg GE administrated mice were significantly increased compared to those in KA-treated mice.GAD-67 and KCC2 play an important role in regulating GABAergic system. Our results suggest that GE has anti-convulsant effect against KA-induced epileptic seizure through enhancing GABAergic system.

• Korean Journal of Acupuncture
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• v.28 no.2
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• pp.23-36
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• 2011

목적 : 이 연구는 관절 및 심혈관계 질환 치료에 사용되는 송절(松節)(Pinus densiflora Gnarl)을 약침용 시료로 조제하여 본 약물의 항산화 효능을 규명하고자 하였으며 이를 다양한 시스템에서 검토하였다. 방법 : $FeCl_2$-ascorbic acid system에서 흰쥐 간조직의 지질과산화 반응을 관찰하였고, Fenton reaction system에서 자유기에 의한 plasmid DNA 분절을 유도하였다. 또한 deoxyribose assay를 통해 hydroxyl radical 소거능을 관찰하였고, NBT reduction assay로 superoxide radical 소거능을 검토하였다. 또한 human low-density lipoprotein(LDL)의 산화를 유도하기 위해 $CuSO_4$와 AAPH를 사용하였으며 relative electrophoretic mobility (REM) assay로 LDL 산화 억제 효능을 대조 항산화물질과 비교 검토하였다. 결과 : 송절 약침액은 자유기에 의한 간조직의 지질과산화(p < 0.01)및 DNA 분절을 현저하게 억제하였으며, hydroxyl radical, superoxide radical (p < 0.01), nitric oxide 및 peroxynitrite를 강하게 소거하였다. 또한 $CuSO_4$ ($IC_{50}=9.2{\pm}0.2\;{\mu}g/ml$)와 AAPH ($IC_{50}=34.8{\pm}5.1\;{\mu}g/ml$)에 의해 유도된 human LDL의 산화를 억제하였고, REM assay에서도 산화 억제 효능을 재확인할 수 있었다. 결론 : 송절 약침액은 활성산소종 및 활성질소종를 소거하였고, 지질과산화를 억제하였으며, 특히 human LDL의 산화적 손상을 방어하였다. 이에 본 약물은 자유기에 의한 심혈관의 산화적 손상을 효과적으로 보호할 것으로 판단된다.