Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Effects of Electroacupuncture on the excitability in Medial Vestibular Nuclei of Rats

흰쥐의 내측 전정신경핵 흥분성에 대한 전침자극의 효과

  • Kim, Jae-Hyo (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Lee, Sung-Ho (Division of Sports and Welfare, Yewon Arts University) ;
  • Sohn, In-Chul (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Kim, Young-Sun (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Kim, Min-Sun (Dept. of Physiology, Wonkwang University School of Medicine)
  • 김재효 (원광대학교 한의과대학 경혈학교실) ;
  • 이성호 (예원예술대학교 스포츠복지학부) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실) ;
  • 김영선 (원광대학교 한의과대학 경혈학교실) ;
  • 김민선 (원광대학교 의과대학 생리학교실)
  • Published : 2009.09.27
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The vestibular system detects head movement and serve to regulate and maintain the equilibrium and orientation of the body. It is known that the vestibular imbalance leads to vestibular symptoms such as nausea, vomiting, vertigo and postural disturbance. The objectives of the present study were to examine a modification of the dynamic activities of medial vestibular nucleus (MVN) neurons following electroacupuncture (EA) of GB43 (Hyepgye). Methods : In Sprague-Dawley rats weighing $250{\sim}300g$, dynamic responses induced by sinusoidal whole body rotation about vertical axis at 0.2 Hz were observed in MVN of rats during EA of GB43 (Hyepgye) with 0.2 ms, 40 Hz and $600{\pm}200{\mu}A$. Also, expression of cFos protein was observed 2 hours after EA for 30 mins. Results : In dynamic response of vestibular neuron, the excitatory or inhibitory responses of gain were predominant in the ipsilateral MVN neurons during EA but not predominant in the contralateral MVN. Most neurons showing decreased gain were classified to inhibitory responses of spontaneous firing discharge during EA and ones showing increased gain were classified to excitatory response of spontaneous firing discharge during EA. Also, EA of the left GB43 (Hyepgye) for 30 mins produced the expression of cFos protein in MVN, inferior olive (IO) and solitary tract nuclei (SOL). Spatial expressions of cFos protein were predominant in the contralateral MVN, ipsilateral IO and bilateral SOL. Conclusion : These results suggest that the excitability of MVN neurons was influenced by EA of GB43 (Hyepgye) and EA may be related to the convergence on MVN.

Keywords

References

  1. 郭靄春 編著. 黄帝内経 霊樞校注語譯. 서울 : 医聖堂. 1993 : 148-60, 268-71, 286-8, 484-500, 537-43.
  2. 郭靄春 編著. 黄帝内経 素問校注語譯. 서울 : 医聖堂. 1993 : 14-28, 55-64, 87-101, 102-36, 137-41, 246-53, 268-71, 346-69, 401-13, 433-68, 470-99.
  3. 이정구. 어지러움. 서울 : 단국대학교출판부. 2001 : 9-32, 33-58, 59-72.
  4. Yates BJ, Miller AD. Physiological evidence that the vestibular system participates in autonomic and respiratory control. Jounal of Vestibular Research. 1998 ; 8 : 17-26. https://doi.org/10.1016/S0957-4271(97)00035-9
  5. Beitz AJ, Anderson JH. Neurochemistry of the vestibular system. New York : CRC Press. 2000 : 3-94.
  6. 馬有度. 實用中医内科學. 上海: 上海科學技術出版社. 1988 : 405-13.
  7. Lee HS, Chung DG. The oriental and western medical study of dizziness. The Journal of East-West Medicine. 1990 ; 15(3) : 5-14.
  8. Park BR, Park HA. A study of the sinusoidal head rotator. Chonnam Med J. 1988 ; 25 : 25-30.
  9. Paxinos G, Watson C. The rat brain in stereotaxic coordinates, 4th edition. London : Academic Press. 1998 : 61-71, 80-82, 96-100.
  10. 呉緒平 等編. 現代穴位療法大全. 北京 : 中國医薬科学出版社. 1997 : 102-5.
  11. 김이화 監修. 鍼灸内科学. 서울 : 木과 金. 1999 : 259-64.
  12. 皇甫謐. 黄帝鍼灸甲乙経. 台北 : 宏業書局有限公司. 1976 : 250, 278.
  13. Park SY, Whang WJ, Eom TS. Study of oriental medical science documentary records of vertigo and dizziness. The Journal of Korean Acupuncture & Moxibustion Society. 1990 ; 7(1) : 369-79.
  14. Wilson VJ, Wylie RM, Marco LA. Projection to the spinal cord from the medial and descending vestibular nuclei of the cat. Nature. 1967 ; 215(99) : 429-30. https://doi.org/10.1038/215429a0
  15. Yates BJ, Grelot L, Kerman IA, Balaban CD, Jakus J, Miller AD. Organization of vestibular inputs to nucleus tractus solitarius and adjacent structures in cat brain stem. Am. J. Physiol. 1994 ; 267(4 Pt2) : R974-83.
  16. Balaban CD, Thayer JF. Neurological bases for balance-anxiety links. J Anxiety Disord. 2001 ; 15(1-2) : 53-79. https://doi.org/10.1016/S0887-6185(00)00042-6
  17. Kim MS, Kim JH, Kry D, Choi MA, Choi DO, Cho BG, Jin YZ, et al. Effects of acute hypotension on expression of cFos-like protein in the vestibular nuclei of rats. Brain Res. 2003 ; 962(1-2) : 111-21. https://doi.org/10.1016/S0006-8993(02)03977-X
  18. Kennedy RS, Graybiel A, McDonough RC, Beckwith FD. Symptomatology under storm conditions in the North Atlantic in control subjects and in persons with bilateral labyrinthine defects. Acta Otolaryngol. 1968 ; 66(6) : 533-40. https://doi.org/10.3109/00016486809126317
  19. Lee KH, Jeong JW, Park OK, Lee MY, Kim MS, Park BR. Role of vestibulosympathectic reflex on orthostatic hypotension in rats. Korean Circ J. 1998 ; 28 : 998-1006. https://doi.org/10.4070/kcj.1998.28.6.998
  20. 許浚 著, 東医宝鑑國譯委員會 譯. 對譯東医宝鑑. 서울 : 법인문화사. 2002 : 575-89.
  21. 黄麗春. 耳穴診斷治療學. 서울 : 医聖堂. 1993 : 1-15.
  22. Shiraishi T, Onoe M, Kojima T, Sameshima Y, Kageyama T. Effects of auricular stimulation on feeding-related hypothalamic neuronal activity in normal and obese rats. Brain Res. Bull. 1995 ; 36(2) : 141-8. https://doi.org/10.1016/0361-9230(94)00179-5
  23. 王富春 洪杰. 経穴治病明理. 北京 : 科学技術文献出版社. 2000 : 300-1.
  24. Kim JH, Park BR, Sohn IC. Electroacupuncture of GB43 modulates neuronal activities in medial vestibular nuclei of rats. Journal of Meridian & Acupoint. 2008 ; 25(3) : 117-35.
  25. Chiu JH, Cheng HC, Tai CH, Hsieh JC, Yeh TC, Cheng H, Lin JG, Ho LT. Electroacupuncture-induced neural activation detected by use of manganese-enhanced functional magnetic resonance imaging in rabbits. Am. J. Vet. Res. 2001 ; 62(2) : 178-82. https://doi.org/10.2460/ajvr.2001.62.178
  26. Tropnikova GK. Effect of the vestibular nuclei of the medulla oblongata on the serotoninergic system function of the brain and small intestine in the rat. Fiziol Zh SSSR Im I M Sechenova. 1984 ; 70(3) : 284-90.
  27. Raitses VS, Shliakhovenko AA. Modulation of the activity of neurons of Deiter's nucleus upon electric stimulation of the hypothalamus and limbic structures of the brain. Fiziol Zh SSSR Im I M Sechenova. 1978 ; 64(8) : 1105-11.
  28. Naranjo JR, Mellstrom B, Achaval M, Sassone-Corsi P. Molecular pathways of pain; fos / jun-mediated activation of a non-canonical AP-1 site in the prodynorphin gene. Neuron. 1991 ; 6 : 607-17. https://doi.org/10.1016/0896-6273(91)90063-6
  29. Mittelstaedt H. Somatic graviception. Biol. Psychol. 1996 ; 42(1-2) : 53-74 https://doi.org/10.1016/0301-0511(95)05146-5
  30. Yates BJ, Jian BJ, Cotter LA, Cass SP. Responses of vestibular nucleus neurons to tilt following chronic bilateral removal of vestibular inputs. Exp Brain Res. 2000 ; 130(2) : 151-8. https://doi.org/10.1007/s002219900238
  31. Jian BJ, Shintani T, Emanuel BA, Yates BJ. Convergence of limb, visceral, and vertical semicircular canal or otolith inputs onto vestibular nucleus neurons. Exp. Brain Res. 2002 ; 144 : 247-57. https://doi.org/10.1007/s00221-002-1042-8
  32. Rubin AM, Liedgren SR, Miline AC, Young JA, Fredrickson JM. Vestibular and somatosensory interaction in the cat vestibular nuclei. Pflugers Arch. 1977 ; 371(1-2) : 155-60. https://doi.org/10.1007/BF00580784
  33. Rubin AM, Liedgren SR, Odkvist LM, Larsby B, Aschan G. Limb input to the cat vestibular nuclei. Acta Otolaryngol. 1979 ; 87(1-2) : 113-22. https://doi.org/10.3109/00016487909126395
  34. Rubin AM, Liedgren SR, Odkvist LM, Milne AC, Fredrickson JM. Labyrinthine and somatosensory convergence upon vestibulospinal neurons. Acta Otolaryn gol. 1978 ; 86(3-4) : 251-59. https://doi.org/10.3109/00016487809124744