Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effects of Prunella vulgaris Pharmacopuncture on Lipopolysaccharide-Induced Acute Inflammatory Rat Model

하고초 약침이 LPS로 유발된 급성염증 백서 모델에 미치는 영향

  • Lee, Jong-Wook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university) ;
  • Lee, Hyang-Sook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of healt Science, Sangji university) ;
  • Lee, Joon-Moo (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university)
  • 이종욱 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이향숙 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 이준무 (상지대학교 한의과대학 경락경혈학교실)
  • Published : 2009.09.27
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Abstract

Objectives : To investigate the anti-inflammatory effects of Prunella vulgaris pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 5 groups; normal control (n=8), LPS control (n=8), LPS+Prunella vulgaris pharmacopuncture at CV4 (CV4, n=8), LPS+Prunella vulgaris pharmacopuncture at ST36 (ST36, n=8), and LPS+Prunella vulgaris pharmacopuncture at CV12 (CV12, n=8). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5mg/kg). Proinflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-10 (IL-10), thiobarbituric acid reactive substance (TBARS) from blood and liver tissue were compared before and 5 hrs after inflammation induction. Results : In CV4 and CV12 groups, plasma IL-$1{\beta}$, IL-6 and TNF-$\alpha$ levels increased by LPS injection, significantly decreased 5 hrs after injection (p<0.05). For CV12 group, plasma IL-10 concentration significantly increased (p<0.05). Liver IL-$1{\beta}$ and IL-6 levles significantly decreased in CV4 and CV12 groups (P<0.05), while normal and LPS control groups were not significantly different in TNF-$\alpha$ and IL-10 levels. Plasma TBARS concentration was significantly decreased in CV12 group, while there was no significant difference among LPS control and pharmacopuncture groups for liver TBARS concentration. Conclusions : Based on the present findings, Prunella vulgaris pharmacopuncture at CV12 may have a potentially preventive anti-inflammatory effect in an LPS-induced inflammatory rat model.

Keywords

References

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