• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.444-448
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• 2002

A $CaCO_3$-alginate beads system was developed for high cell density cultivation of Bifidobacterium longum and the cost-effective media were also screened. In batch process with $CaCO_3$, beads, two strains of B. longum showed both the highest viable cells and optical density in TPY medium, resulting in maximum optical density and viable cell counts of 12.40, $2.22{\times}10^10$ cfu/ml for B. longum ATCC 15707 and 13.71, $3.93{\times}10^10$ cfu/ml for B. longum HLC 3742. Released size distribution, according to $CaCO_3$-alginate bead size preparation, was smaller than others. These results were also examined by observing their morphology. The skim milk-based medium was most adequate to cultivate B. longum as the cheapest medium, and $10\%$ skim milk supplemented with $2\%$ glucose and $1\%$ yeast extract was a suitable medium, supporting the growth to $5.57{\times}10^10$ cfu/ml for ATCC 15707 and $6.82{\times}10^9$ cfu/ml for HLC 3742. During the long-term storage at $4^{\circ}C\;and\;-20{\circ}C$, B. longum cultivated with $CaCO_3$ beads had the highest stability. Consequently, $CaCO_3$-alginate beads buffer was found to be useful not only to cultivate B. longum but also to preserve cultures.

• Journal of Korea Port Economic Association
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• v.37 no.3
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• pp.19-34
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• 2021

Yeosu Gwangyang Port, along with Busan Port, Incheon Port, Ulsan Port, and Pyeongtaek Dangjin Port, serves as Korea's top five ports for trade on the southern coast. It is the second largest port in Korea after Busan Port, and the largest port in terms of import and export volume. Yeosu Chemical Industrial Complex, the world's largest chemical industrial complex, has continued to grow rapidly, but recently, the increase in volume has been decreasing. Therefore, this study sought to find major development strategies for the development of Yeosu Gwangyang Port and to derive the priorities of the strategies. To this end, the development strategy of Yeosu Gwangyang Port was divided into three major categories: operation revitalization, infrastructure construction, and policy support using the AHP analysis technique and analyzed again in two aspects: short, medium, and long term. As a result of the analysis, 'integrated operation of container docks and strengthening competitiveness' were considered the most important in short- and medium-term policies. It is believed that it will be necessary to integrate container operators, establish routes in preparation for entry of super-large ships, and install large cranes. In the long-term policy, the most important thing was to foster high value-added industries based on local industries. It is believed that strategies are needed to attract companies from outside regions through the settlement support system. The results of this study are expected to be used to establish development strategies for Yeosu Gwangyang Port and to establish investment priorities.

• Magazine of the Korean Society of Agricultural Engineers
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• v.29 no.4
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• pp.59-72
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• 1987

Although long-term runoff analysis is important as much as flood analysis in the design of water works, the technological level of the former is relatively lower than that of the latter. In this respect, the precise estimation model for the volume of successive runoff should he developed as soon as possible. Up to now, in Korea, Gajiyama's formula has been widely used in long-term runoff analysis, which has many problems in applying in real situation. On the other hand, in flood analysis, unit hydrograph method has been exclusively used. Therefore, this study aims at trying to apply unit hydrograph method in long-term runoff analysis for the betterment of its estimation. Four test catchment areas were selected ; Maesan area in Namlum river as a representative area of Han river system, Cheongju area in Musim river as one of Geum river system, Hwasun area in Hwasun river as one of Yongsan river system, and Supyung area in Geum river as one of Nakdong river system. In the analysis of unit hydrograph, seperation of effective rainfall was carried out firstly. Considering that effective rainfall and moisture condition of catchrnent area are inside and outside of a phenomenon respectively and the latter is not considered in the analysis, Initial base flow(qb)was selected as an index of moisture condition. At the same time, basic equation(Eq.7) was established, in which qb can take a role as a parameter in relating between cumulative rainfall(P) and cumulative loss of rainfall(Ld). Based on the above equation, computer program for estimation model of qbwas seperately developed according to the range of qb, Developed model was applied to measured hydrographs and hyetographs for total 10 years in 4 test areas and effective rainfall was estimated. Estimation precision of model was checked as shown in Tab- 6 and Fig.8. In the next stage, based on the estimated effective rainfall(R) and runoff(Qd), a runoff distribution ratio was calculated for each teat area using by computerised least square method and used in making unit hydrographs in each test area. Significance of induced hydrographs was tested by checking the relative errors between estimated and measured runoff volume(Tab-9, 10). According to the results, runoff estimation error by unit hydrograph itself was merely 2 or 3 %, but other 2 or 3 % of error proved to be transferred error in the seperation of effective rainfall. In this study, special attentioning point is that, in spite of different river systems and forest conditions of test areas, standardized unit hydrographs for them have very similar curve shape, which can be explained by having similar catchinent characteristics such as stream length, catchinent area, slope, and vegetation intensity. That fact should be treated as important factor ingeneralization of unit hydrograph method.

• Journal of the Economic Geographical Society of Korea
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• v.25 no.4
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• pp.600-616
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• 2022

This study contemplates spatial context of startup in manufacturing, mainly analyzing job creation effects of time lag of startups in manufacturing at different technology levels. Using DID model, we found that each region including capital, non-capital and metropolitan area shows different job creation effects of time lag. In capial region, startup cohort in high R&D intensity manufacturing was found to show short-term job creation effects, but in non-capital region, long-term job creation effects was found with the one in medium-high R&D intensity manufacturing. In case of metropolitan area, we couldn't find much evidence of job creation effects that was statistically significant. The result of analysis implied that, in capital region, startup support policies, targeting at high R&D intensity manufacturing, ought to be focused on scale-up of startups that survived for a certain period. And non-capital area and some of metropolitan areas in non-capital region that have comparatively inferior infrastructure and brain-drain problems as well should focus on fostering startups in medium-high R&D intensity manufacturing in a long-term perspective and utilize their traditional manufacturing base.

• International journal of steel structures
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• v.18 no.4
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• pp.1252-1264
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• 2018

Long span bridges such as steel cable stayed and suspension bridges are usually more flexible than short to medium span bridges and expected to have large deformations. Deflections due to live load for long span bridges are important since it controls the overall heights of the bridge for securing the clearance under the bridge and serviceability for securing the comfort of passengers or pedestrians. In case of sea-crossing bridges, the clearance of bridges is determined considering the height of the ship master from the surface of the water, the trim of the ship, the psychological free space, the tide height, and live load deflection. In the design of bridges, live load deflection is limited to a certain value to minimize the vibrations. However, there are not much studies that consider the live load deflection and its effects for long span bridges. The purpose of this study is to investigate the suitability of live load deflection limit and its actual effects on serviceability of bridges for steel cable-stayed and suspension bridges. Analytical study is performed to calculate the natural frequencies and deflections by design live load. Results are compared with various design limits and related studies by Barker et al. (2011) and Saadeghvaziri et al. (2012). Two long span bridges are selected for the case study, Yi Sun-Sin grand bridge (suspension bridge, main span length = 1545 m) and Young-Hung grand bridge (cable stayed bridge, main span length = 240 m). Long-term measured deflection data by GNSS system are collected from Yi Sun-Sin grand bridge and compared with the theoretical values. Probability of exceedance against various deflection limits are calculated from probability distribution of 10-min maximum deflection. The results of the study on the limitation of live load deflection are expected to be useful reference for the design, the proper planning and deflection review of the long span bridges around the world.

• The Journal of Small Business Innovation
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• v.19 no.2
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• pp.37-52
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• 2016

Making efforts to break down the barriers between the intra-company departments as well as cooperating with external partners can become the driving force to create a successful innovation in the new product development (NPD) process. This study deals with how the key factors of collaborative innovation success are working in the process of NPD collaboration. Using case analysis of the NPD process, where a large retailer and small and medium-sized suppliers cooperate, we found that the small and medium-sized suppliers achieved greater 'short-term' performances in the collaboration than the large firm, although the long-term performance is not clear. Among the six antecedents of innovation success, relationship-specific investment played a critical role in motivating the supplier's participation in the NPD process. Adopting a 'closed' network in which the two suppliers interact directly with each other and create new knowledge for the NPD process played an important role in producing a quality product in a reduced development time. Unlike previous studies about the retailer-supplier cooperation for NPD in the food industry suggesting that position differences cause communication problems which is a major obstacle to the NPD success. This study suggests that large retailer's initiative role is a critical success factor in the NPD by the cooperation between small and medium-sized suppliers and large retailers.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.32 no.2
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• pp.13-20
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• 2009

The purpose of this paper is to give information on e-CPC implementation in the manufacturing industry by studying and analyzing a small and medium sized manufacturing company. After analyzing Various factors on J's e-CPC implementation such as environmental factors, process management factor, and result factors, success factors on J's e-CPC implementation are analyzed. The results of this paper can be summarized as follows. First, the most leading role on J's ERP implementation is the will and support of CEO and team leaders. They led the implementation to be stability successfully by settling the different opinions among teams, showing their ability on process decision and communication with consultants. Second, J tried to prompt system stability by mapping out and conducting the long term change management strategy. Third, J organized ERP project team to implement ERP quickly, and it turned out to be successful Finally, J tried to get outside information from consultants and collaborative companies that have experience in implementing ERP. Especially J made full use of overseas consultants. In respect that this paper giver lots of information on e-CPC implementation in manufacturing industry by a case study of a small and medium sized manufacturing company which has not been carried out so far, it would be useful enough.

• Journal of The Korean Society of Agricultural Engineers
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• v.50 no.6
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• pp.3-12
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• 2008

It is important to estimate accurate effective rainfall to analyse flood flow and long-term runoff for the rational planning, design, and management of water resource. The initial abstraction is also important to estimate effective rainfall. The Soil Conservation Service (SCS) has developed a procedure and it has been most commonly applied to estimate effective rainfall. But the SCS method still has weak points, because of unnatural assumptions such as antecedent moisture conditions and initial abstraction. The coefficient of initial abstraction(K) is depending on the soil moisture condition and antecedent rainfall. The maximum storage capacity of Umax which is calibrated by stream flow data in the proposed watershed was derived from the DAWAST(DAily WAtershed STreamflow) model. The values of K obtained from 69 storm events at the five watersheds are ranging from 0.133 to 0.365 and its mean value is 0.207. Effective rainfall could be estimated more reasonably by introducing new concept of initial abstraction. The equation of $K=0.076Sa^{0.255}$ was recommended instead of 0.2 and it could be applicable to the small-medium rural watersheds.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.88-94
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• 2016

Vehicular traffic on turf results in loss of green cover due to direct tearing of shoots and indirect long-term soil compaction. Protection of turfgrass crowns from wear could increase the ability of turf to recover from heavy traffic. Plastic turfpavers have been installed in trafficked areas to reduce soil compaction and to protect turfgrass crowns from wear. The objectives of this study were to evaluate traffic performance of turfgrasses (Zoysia matrella and Axonopus compressus) and soil mixture (high, medium and low sand mix) combinations on turf-paver complex. The traffic performance of turf and recovery was evaluated based on percent green cover determined by digital image analysis and spectral reflectance responses by NDVI-meter. Bulk density cores indicated significant increase in soil compaction from medium and low sand mixtures compared to high sand mixture. Higher reduction of percent green cover was observed from A. compressus (30-40%) than Z. matrella (10-20%) across soil mixtures. Both turf species displayed higher wear tolerance when established on higher sand (>50% sand) than low sand mixture. Positive turf recovery was also supported by complementary spectral responses. Establishment of Zoysia matrella turf on turfpaver complex using high sand mixture will result in improved wear tolerance.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.689-695
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• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.