• Nutrition Research and Practice
• /
• 제14권2호
• /
• pp.102-108
• /
• 2020

BACKGROUND/OBJECTIVES: In Oriental medicine, certain foods may be beneficial or detrimental based on an individual's constitution; however, the scientific basis for this theory is insufficient. The purpose of this study was to investigate the effect of body constitution, based on the Sasang type of Korean traditional medical classification system, on the bioavailability of soy isoflavones of Cheonggukjang, a quick-fermented soybean paste. SUBJECTS/METHODS: A pilot study was conducted on 48 healthy Korean men to evaluate the bioavailability of isoflavone after ingestion of food based on constitution types classified by the Sasang typology. The participants were classified into the Taeeumin (TE; n = 15), Soyangin (SY; n = 15), and Soeumin (SE; n = 18) groups. Each participant ingested 50 g of Cheonggukjang per 60 kg body weight. Thereafter, blood was collected, and the soy isoflavone metabolites were analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Ntrikinetic analysis of individual isoflavone-derived metabolites was performed. RESULTS: Our nutrikinetic analysis identified 21 metabolites derived from isoflavones in the blood samples from 48 healthy Korean men (age range, 21-29 years). Significant differences were observed in the time to maximum concentration (T_max) and elimination half-life (t_1/2) for nine metabolites among the three groups. The T_max and t_1/2 of the nine metabolites were higher in the SE group than in the other groups. Moreover, the absorption rates, as determined by the area under the plasma-level curve (AUC) values of intact isoflavone, were 5.3 and 9.4 times higher in the TE group than in the SY and SE groups, respectively. Additionally, the highest AUC values for phase I and II metabolites were observed in the TE group. CONCLUSIONS: These findings indicate that isoflavone bioavailability, following Cheonggukjang insgestion, is high in individuals with the TE constitution, and relatively lower in those with the SE and SY constitutions.

• 대한지역사회영양학회지
• /
• 제2권3호
• /
• pp.338-348
• /
• 1997

This study was conducted to investigate the health and nutritional status of 123 middle aged men at their worksite in Taejon. The results of this study on the factors that influence their health and nutritional status were as follows : 1) 74.8$\%$ of the subjects had history in the order of alimentary, heat, liver, diabetic and pulmonary diseases. 30.3$\%$ stopped smoking at 42.3% yrs. and 74.5$\%$ smoked more than 10 cigarets per day. Also 71.9$\%$ drank 2-3 times per week and 35.3$\%$ drank 1-2 times per week. 91.4$\%$ exercised more than 30min every day. 2) 54.4% showed concerns about their health whereas 20.3$\%$ were afraid that they night get sick. 3) 90.4$\%$ ate regularly and 54.5$\%$ worried about their cholesterol, salt, fat and MSG intakes. 48.7$\%$ ate out 1-2times per week and their favorite foods eaten outside were Korea. 4) 41.5$\%$ were classified as 'normal A', 30.9$\%$ 'normal B' group and high blood pressure and liber diseases in 'doubtful for disease' group were pointed out from their 1996 health check ups. 5) By Broca index, 39.8$\%$ were overweight and 9.8% were obese however by BMI only 23.6% were overweight. According to the relationship between calculated and self recognized obesity, 62.4$\%$ categorized themselves into the right weight range but 34.3$\%$ thought they were thinner than they were. 6) 43.9$\%$ were border line in cholesterol intake and 12.1$\%$ needed medical care for high blood cholesterol. 7) The Average energy intake was 1970.6㎉(80.9$\%$ RDA) with a 65 :19 : 16 ratio of carbohydrate : protein : fat. Protein, Fe, thiamin, riboflavin, niacin and Vit. A. 8) Occupation, regularity of meals, partner's job, income. smoking, alcohol drinking, health concerns and eating out were the factors that influenced the subject's nutrient intakes and health status. from this study, it was found that middle aged men needs to know their health and nutritional status and to be educated correct health and nutritional information through formal or informal channel. The worksite is the vest place to do this and we want these results to be used to develop the nutrition education program for middle aged men at the worksite.

• 한국식품영양학회지
• /
• 제29권1호
• /
• pp.128-133
• /
• 2016

본 연구에서는 돼지감자 꽃, 잎, 뿌리가 가진 기능성 식품 소재로서의 활용도를 높이고자 다양한 생리활성을 평가하였다. 돼지감자 꽃, 잎, 뿌리의 폴리페놀 함량은 32.80 mg GAE/extract g, 43.46 mg GAE/extract g, 24.53 mg GAE/extract g으로 나타났으며, 플라보노이드 함량은 각각 15.64, 18.67, 11.65 mg GAE/extract g으로 나타났다. 돼지감자 꽃, 잎, 뿌리의 메탄올추출물의 DPPH 라디칼 소거능 활성은 각각 77.78%, 66.07%, 26.93%로 나타났으며, hydroxyl radical 소거능 활성은 각각 55.33%, 38.30%, 34.89%로 나타났다. 돼지감자 꽃, 잎, 뿌리메탄올 추출물의 아질산염 소거능을 측정한 결과, 돼지감자 추출물의 농도가 증가함에 따라 그 활성 역시 증가하는 경향으로 나타났다. 돼지감자 부위별 메탄올 추출물(5 mg/mL)의 ${\alpha}-glucosidase$ 저해활성은 꽃, 잎, 뿌리에서 각각 41.08%, 55.67%, 60.76%로 유의적인 차이를 나타났으며, ${\alpha}-amylase$ 저해활성은 꽃, 잎, 뿌리에서 각각 49.95%, 59.88%, 66.25%로 유의적인 차이를 나타내었다.

• 생명과학회지
• /
• 제29권12호
• /
• pp.1408-1414
• /
• 2019

효소는 세제, 식품, 사료, 의약품 및 의료용 분야 등 산업 전반적인 응용 분야에서 널리 사용되고 있으며, 산업 제품 및 공정에서 주요 요인이다. 효소를 선별, 확인, 및 특성 분석을 위해 zymography 기술이 일상적으로 사용됩니다. Zymography 기술은 SDS-전기영동을 통해 단백질을 분리한 후 포함된 기질을 겔 상에서 분해하는 기능성 효소를 검출하는 데 널리 사용되는 단순하고 민감하며 정량화가 가능한 기술이다. 이 방법은 비 환원 조건하에서 SDS-전기영동 겔에서 전기영동에 의한 단백질의 분리와 겔 상에서 효소 활성을 검출하는 다목적 2 단계의 기술이다. 이는 SDS-전기영동 겔에 기질을 중합시키고 전기영동 분리 후 효소 반응 완충용액에서 복원된 가수분해 효소에 의해 분해되는 것을 기반으로 하는 기술이다. 미생물 배양액, 식물 추출물, 혈액, 조직 배양액, 식품 속 효소 및 메타 프로테옴을 포함한 어떤 종류의 생물학적 시료들을 zymography에 적용하고 분석이 가능하다. Zymography의 장점은 전처리 없이 혼합된 시료를 적용하여 SDS-전기영동 겔 상에서 활성을 지닌 단백질을 직접 육안으로 검출이 가능할 뿐만 아니라 나노그람(nanogram) 수준에서 활성을 확인이 가능하다. 그래서 이 zymography 기술은 다양한 분야에 응용이 가능하다. 하지만, 이러한 장점이 오히려 단점으로 작용하여 실험적 오류를 범할 수 있는 경우가 많다. 본 총설에서 zymography 기술의 장점, 단점, 및 문제점 해결에 관해서 서술하였다.

• 한국식품과학회지
• /
• 제36권6호
• /
• pp.995-1000
• /
• 2004

뽕나무 가운데 가장 큰 품종인 4배성 휘커스 오디품종(Ficus-4x)의 일반성분함량을 베리류에 속하는 몇 종류의 과일과 비교시 유사한 수준이었으나, 조회분 함량은 딸기, 복분자, 블루베리 등에 비해 높은 수준을 보였다. 비타민 C함량의 경우도 복분자와 블루베리 보다 높은 수준이었다. 무기질함량 분석결과 칼슘함량은 딸기와 유사한 수준이었으나, 블루베리에 비해서는 2배 이상 높았다. 특히 철분함량은 딸기, 복분자, 블루베리에 비해 월등히 높게 나타나 한방에서 보혈제로 사용해온 근거가 본 연구를 통해 입증되었다고 볼 수 있다. 물성적 특성의 경우 응집성, 검성, 씹힘성이 딸기에 비해 높게 나타나 저장성 면에서 딸기에 비해 우수할 것으로 생각된다. 색도 분석 결과 명도가 낮게 나타나 딸기보다 비교적 어두운 붉은색을 띠었고, 적색도, 황색도 또한 딸기에 비해 다소 낮은 결과를 나타내었다. 4배성 휘커스오디품종의 항산화능을 검색하고자 DPPH소거능 검색시 BHA를 기준(100%)으로 했을때 오디메탄올추출물은 35.7%로 cyanidin 3-glucoside의 40%의 소거능력을 보였다. 그리고 유지 저장시 항산화능을 알아본 결과 옥수수기름 저장 15일 이후부터는 오디의 에탄올추출물과 물추출물 모두에서 토코페롤과 BHA 보다도 안정한 경향을 나타내었다. 이는 최근 천연물에서 추출된 유리한 성분을 첨가물로 이용하려는 목적과 잘 일치된다고 할 수 있으며 유지의 유도기간을 연장하여 장기 보존을 위한 항산화제로서의 가치가 있다고 사료된다.

• 한국식생활문화학회지
• /
• 제5권3호
• /
• pp.331-347
• /
• 1990

This study was designed under the purpose to analyze the Korean Traditional Folk Remedies on food, to submit the basic statistical data, and to utilize them by examining how much they know about the Korean Traditional Folk Remedies and also by examining how frequently they used them. The sample was consisted of 312 housewives living in Daegu, Kyungsan city and Kyungsan-eup, the survey was made by use of questionaires from July 15 to August 1 1989. The results are as follows 1. Analysis of Folk Remedical Contents 1) The method of the Folk Remedies used in this area was mainly food, which can be easily found around their house and easy to use, it was possible to apply scientific principles in some cases. 2) The major popular method was shown to feed boiled barley or brown rice which are known as the Folk Remedy for Diabetes. 3) The ANOVA of variable shows the significant influence on each object which age, religion, the level of education, job, monthly income, and the area except family formation. 2. The Frequency of Folk Remedies and additional analysis 1) The most frequently used Folk Remedy was Arterio-sclerosis(1.44), which is followed by the loss of Eyesight(1.40), Hang over(1.28), Couth(1.27), Cold(1.26) etc. 2) In the analysis of Pearson Correlation between frequencies of Folk Remedies used, and demographic variables such as age(p<.05), the monthly income(p<.01), in that area, total number of response items showed a positive correlation. 3. The source to learn about Folk Remedy. 1) It is acknoledged that they are mostly instructed by their forefathers, friends, neighbors, professional textbooks, mass communications, herb doctors, other medical sources or education at school etc. 2) The ANOVA of Variables shows the big differences between each group by age. Based on the above findings, the following suggestions are made ; Most of Korean Traditional Folk Remedies are recognized scientific and reasonable which are based on the scientific research and herb medicine therefore it should be made good use of for our life in good health additionally. I hereby insist that the importance of our Folk Remedies should be reviewed and focused for maintenance our health.

• 한국미생물·생명공학회지
• /
• 제39권3호
• /
• pp.308-312
• /
• 2011

Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.

• 사상체질의학회지
• /
• 제8권2호
• /
• pp.41-68
• /
• 1996

This thesis is a try for examining the historical characteristics of Constiutional medicine. For this purpose, I have examined their theory, and made a comparison carefully. Through this study, I have obtained several results as following. Both Chang and Lee lived in the time of change, which was unstable and uncertain. Under the condition, they both rejected following the existing trends of learned circles, and developed new thoughts and clinical techniques. They rejected superstitious beliefs and fatalism, and conducted the pursuit of experimental knowledge and rationale idea. Clinical experience was the very base of their study. They both criticized the bad habits of abuse of tonifing medication and health seeking. Especially Lee regarded control of emotion and regulation of a way of life as the best way for preservation of one's health. Chang regarded pathogenic factors as the ultimate factor of diseases, and strived for eliminating pathogenic factors, but L brought to a conclusion that the final factor of diseases was what is called "heart" itself, and emotional changes were the most essential causes of disease. It can be said that the pathogenesis insisted by Chang can be called The insistence that pathologic factors are the very etiology of all the disease (邪氣致病論), or all the diseases result from pathologic factors. And his whole remedy can be summarized as following, A study on the method of eliminating pathogenic factors. But the purpose of Constitutional medicine is to correct imbalance intrinsic to one's internal organs. In this aspect, Constitutional medicine is a "regulatory medicine". Depending on the classification of six vital substances, Chang classified all disease into six categories. These were based on symptoms and sings represented. While classification of diseases made by Lee was likely to rely upon constitutional characteristics. Chang thought that the three remedies made up of perspiration, purgation, vomiting were the most efficient way of eliminating pathogenic factors. Lee, however, thought those weren't methods of eliminating pathogenic factors but the best ways restoring one's self-regulation power. Chang thought that all the febrile disease essentially has a tendency in properties to belong to "heat", but Lee pointed out that pathologic processes are variable in accordance with constitutional features. They both regarded pathogenesis of diabetes as fire. That is to say, fire is the most essential factor of diabetes. And there are many risk factors such as inappropriate foods, drugs, climate, etc., but Lee thought what is most important is heart. Putting all accounts together, medical characteristics of Chang are similar to those of T aiyinjen and Shaoyangjen, and have no relation to those of those of Shaoyinjen. Therefore we can conclude that Chang understood pathologic processes of disease of T aiyinjen and Shaoyangjen, whether he knew about constitutional features or not.

• 대한지역사회영양학회지
• /
• 제23권5호
• /
• pp.397-410
• /
• 2018

Objectives: An association between dietary patterns and mental health in children has been suggested in a series of studies, yet detailed analyses of dietary patterns and their effects on ADHD (attention deficit hyperactivity disorder) are limited. Methods: We included 4569 children who had dietary intake data as part of the CHEER (Children's Health and Environmental Research) study conducted nationwide from 2005 to 2010. We assessed ADHD (Attention Deficit Hyperactivity Disorder) by the DuPaul's ADHD Rating Scales and dietary intake by a semi-quantitative food frequency questionnaire. Using intake data, we constructed five dietary patterns: "Plant foods & fish," "Sweets," "Meat & fish," "Fruits & dairy products," and "Wheat based." Results: The overall proportion of ADHD was 12.3%. Boys (17.8%) showed a higher rate of ADHD than girls (6.5%). The total intake of calories (85 kcal) and plant fat (2g) in the ADHD group was significantly higher than that of the normal group. ADHD was significantly negatively associated with dietary habits such as having breakfast and meal frequency, and positively associated with eating speed, unbalanced diet, overeating, and rice consumption. Regarding dietary patterns, the "Sweets" category was relevant to high ADHD risk (OR 1.59, 95% CI: 1.18, 2.15 for Q5 vs. Q1) in a linear relationship. An inverse, non-linear association was found between "Fruits & dairy products" and ADHD (OR 0.55, 95% CI: 0.39, 0.76 for Q4 vs. Q1). Conclusions: Our study confirms both positive and negative associations between diet and ADHD in elementary school age children. Moreover, linear or nonlinear associations between diet and ADHD draw attention to the possible threshold role of nutrients. Further studies may consider characteristics of diet in more detail to develop better intervention or management in terms of diet and health.

• 생명과학회지
• /
• 제22권12호
• /
• pp.1704-1711
• /
• 2012

미생물 발효에 의한 부위별 가시오가피의 생리활성 작용 및 물질을 개발하기 위한 기초 자료를 얻을 목적으로 Aspergillus oryzae KCCM 11372, Aspergillus kawachii KCCM 32819, Monascus purpureus KCCM 12002 3종의 곰팡이 균주를 본 실험에 사용하였으며, 이들에 의한 부위별 발효 가시오가피의 폴리페놀, 플라보노이드와 미네랄 함량, 지방산 조성, 항산화 활성, 미백 효능을 비교 검토하였다. 곰팡이 균주로 발효시킨 분말의 pH범위는 3.9-6.97 였으며, 미네랄 성분 조성 비율은 Ca, K, Mg 순으로, 각 곰팡이 균주 발효에 의한 발효 가시오가피 분말의 미네랄 함량은 전체적으로 발효된 분말이 발효 전 분말보다 함량이 높았다. 주요 지방산 조성은 palmitic acid, stearic acid, oleic acid 으로 전체적으로 포화지방산이 대부분을 차지하고 있었다. 항산화 활성은 가시오가피 줄기에서 발효전 분말은 약 27%의 활성을 나타냈지만 M. purpureus와 A. oryzae 균주로 발효시킨 분말은 약 73%와 61%로 활성이 증가했다. 또한, 가시오가피 열매도 발효전 분말은 약 58%의 활성을 나타냈지만 M. purpureus와 A. oryzae 균주로 발효시킨 분말은 약 77%와 69%로 활성이 증가했다. 미백 효능은 가시오가피 잎, 뿌리, 줄기, 열매는 약 20% 이하로 매우 낮은 수준이었지만 가시오가피 잎과 뿌리에서 발효에 의해 활성이 증가하는 경향을 보였다. 이상의 실험결과에서 곰팡이 균주에 의한 부위별 발효 가시오가피의 생리활성 효과 및 이화학적 특성이 발효 전 가시오가피 분말보다 강화됨으로써 건강기능식품이나 화장품의 천연 항산화제 소재로서의 이용 가능성이 높다고 할 수 있다.