• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.81-88
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• 2015

We investigated factors affecting primary cultures of Pacific abalone Haliotis discus hannai ovary-dissociated cells to identify general aspects of their early-phase culture. Ninety-seven cell populations derived from 30 individuals were cultured in different media with varying compositions of medium supplements, and initial attachment, subculture, and survival for ${\geq}10$ weeks were assessed according to medium composition and individual. We also examined the time required for subculture and the rate of cell death according to both culturing period and passage number within 10 weeks. A lack of fetal bovine serum (FBS) and hemolymph significantly inhibited the growth of cultured cells, while we detected no significant effect of medium composition on initial cell attachment. Through data reallocation, with the omission of data from cell populations cultured in FBS-free and hemolymph-free media, we showed that growth inhibition was also affected by individual differences among the abalones used. During the culture, we observed four different types of cell morphology. Moreover, considerable time was required for subculture-18.4 and 19.5 days for first and second subcultures, respectively-and cell death did not occur within 30 days or for passage 0. Our results will provide valuable information for developing universal cell culturing guidelines in abalone species and suggest the feasibility of culturing abalone ovary-dissociated cells.

• 미생물학회지
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• 제40권3호
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• pp.217-220
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• 2004

Cordyceps militaris의 액체 배양시 cordycepin(3'-deoxyadenosine) 생성에 영향을 주는 물리화학적 조건 및 배지 조성의 영향을 조사하였다. 사용된 17종 균주 중에서 C. militaris KCTC 6862, C. militaris KCTC 16932 및 C. militaris DGUM 32003이 우수한 cordycepin생산성을 보여주었다. Cordycepin생산에 적합한 최적 온도와 PH는 각각 $24^{\circ}C$와 pH 6.0-10범위이었다. YM배지에 탄소원으로 glucose를, 질소원으로 tryptone을 각 1% 첨가하였을 매 우수한 cordycepin 생산을 보여주었다. Tryptone을 질소원으로 하는 YMG 액체배지에서 5일간 배양하였을 경우, 39mg/l의 cordycepin의 농도가 측정되었다. 0.1%의 다양한 인산원을 각각 첨가한 결과, cordycepin생산을 저해하였다.

• Corrosion Science and Technology
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• 제2권6호
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• pp.260-265
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• 2003

The experimental methods to rapidly and stably reproduce Microbially Influenced Corrosion (MIC) of stainless steel by sulfate-reducing bacteria such as Desulfovibrio vulgaris were developed. In this study, using two types of stainless steel, 304 and 444, obtained from Pohang Steel & Iron Co., Ltd. (POSCO)., three major factors were tested; overall medium composition, dilution ratio, and chloride concentration. In the overall medium tests, three different media were prepared according to $FeSO_4$ concentration; PM (original Postgate's medium No. 2), MPM 1 (modified PM, no $FeSO_4$, MPM 2 (modified PM, 1/10 $FeSO_4$). The effects of various dilution ratios (3, 1, 1/3, 1/10, 1/30, and 1/100 times) and chloride concentrations (0.0067M, 0.01M, 0.05M, and 0.1M) were examined during 2 months cultivation. Through SEM (Scanning Electron Microscopy) observation, the diluted and modified media, particularly the $1/3{\times}MPM$ I medium, showed more micro-pitting points on surfaces compared to the original PM medium. High concentrations of chloride ions (above 0.05M) were not adequate for observation of MIC since those brought about non-microbiologically induced corrosion. From this study, the optimization of medium composition was very effective to routinely observe MIC in a laboratory system.

• 한국실내디자인학회논문집
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• 제17권3호
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• pp.139-147
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• 2008

Modern architecture's optical mechanism focused on Ocuularcentrism neglects the tactility of vision and tends to eliminate the optical and tactile dualism of traditional spaces by representing spaces and surfaces that are abstract and cold-hearted. In other words, all sensory experiences, except for visual experiences, are eliminated to make it impossible to create the substantial core of architecture that combines time, image, and surface textures. The fast-changing social trends, the emergence of new materials and technologies, and the corresponding development of various types of media since the Industrial Revolution have changed the paradigm of human perception and representation. With the development of media, other sensory experiences besides visual experience have been stressed and human perception has converted from single perspective to complex perspective. In result, new sensory items, such as visual tactility, have replaced the traditional vision-centered hierarchy. The composition of architectural surfaces has represented the functional and commercial needs of technology, structure, as well as the socio-cultural needs of the community. In contemporary times, it is being changed and developed by the new tactility and the corresponding expression of modern architecture. Based on the visual representation of tactility of architectural surface, this study used a composition of surface that combines various events, meanings, and senses to examine how architecture can mediate and reproduce viewers' visual experiences and discover the existential relationship between architecture and men.

• 한국버섯학회지
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• 제11권3호
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• pp.145-148
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• 2013

목이버섯의 톱밥종균을 제조하는데 참나무톱밥은 영양원으로 미강을 20%첨가하고, 포플러톱밥은 밀기울을 10%첨가하였을 때 목이버섯의 균사와 밀도가 양호하였다. 이때 봉지재배에 적합한 종균 접종양은 참나무톱밥종균은 15 g, 포플러톱밥종균은 25 g를 접종할 때 오염발생률이 줄고 배양완성률이 높아 적합한 접종양 이었다.

• 한국실내디자인학회논문집
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• 제16권2호
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• pp.97-104
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• 2007

At a time of emphasized need for user-oriented design, methods such as Post Occupancy Evaluation are being used to measure the exact demands of the users. The conventional methods, however, have mostly been conducted based on questionnaires printed on paper, which has posed numerous problems in researches designed to measure the visual demands. Along with the continued development of the internet and computer media, there have been examples of interactive media applications as a means of improving the existing paper-based research methods. This study is a follow-up to the primary study that unveiled cautious variances of the media. 200 samples of media-based researches were extracted to be used in analysis of in-depth visual demands. 200 housewives from rural Iud development zones, who exercises the most influence in household purchases in these regions where the problem of rate of distribution Is coming under the limelight, were chosen as the subjects. The subjects were selected from land development zones of Gimhae-si and Haeundae. At first, the research was prepared as a web-based endeavor but it employed direct research of field personnel in consideration of that this research is in the beginning. This follow-up study deals mainly with the visual demands for which cautious variances were detected in the primary study and includes analysis of mainly ground Plan layout, space coloration, livingroom utilization, and vlsual composition of LDK. Media research had the advantage of zero rate of questionnaire error due to a pre-programmed search routine, and it was possible to ascertain the actual trade-off basis demands of the residents in terms of ground plan layout. In addition, whereas conventional research methods based on still images were susceptible to the preconceptions of the respondents, the interactive media allowed for easy understanding of the spatial layout and thus made it possible for all respondents to provide answers under the same conditions.

• 한국환경복원기술학회지
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• 제20권5호
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• pp.59-66
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• 2017

Preeminent water treatment plans are essential to preserve the water quality of aquatic biotopes. Previous studies have not been sufficient to provide cost-effective maintenance method since they focused only on the purification of deteriorated water that requires a continuous supply of clean water. This study proposes an economical method of water quality maintenance using water treatment media block constructed vertically using porous ceramics, zeolite, and river pebble. The water treatment media block does not require a separate purification area because it functions as a purifier within the ecological pond which can maximize the biotope area. To evaluate the performance of the water treatment media block, we longitudinally tracked the change of water quality indicators (pH, TDS, COND, DO, T-P, T-N, COD) suggested by Water Environment Standards, Ministry of Environment, Republic of Korea. We compared the water quality of one control (A: general ecological pond composition method of the laminated structure) and two experimental groups (B: a combination of aquatic plants and a water treatment media block, C: a water treatment media block only). As a result, we confirmed that the water treatment media block is an efficient and economical method to maintaining the water quality of the ecological pond for a long time. The water treatment media block will be a great help in providing a better aquatic biotope space for aquatic insects and fishes living in clear water.

• 원예과학기술지
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• 제35권3호
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• pp.289-299
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• 2017

The objective of this study was to determine the effect of replacing perlite (PL) with pellets processed with poultry feather fiber as an inert material to prepare growing medium. The growth and flowering of Salvia splendens 'Vista Red' grown in individual growing medium $Biosangto^{(R)}$, peat moss (PM), PL, and two pellets (P45-1 and P45-2) were evaluated. Peat moss was mixed with PL, P45-1, or P45-2 at various ratios (1:0 to 1:3 or 3:1 by volume) to investigate the feasibility of replacing PL with pellets. Nutrient composition of the growing medium and leaf tissues was analyzed. The number of florets, inflorescence length, plant height, and fresh weight of plants grown in media containing P45-1 or P45-2 were reduced compared to those grown in individual growing medium PM or PL. As the mixing ratio of P45-1 or P45-2 to PM was higher, the growth of salvia, such as inflorescence length, plant height, number of leaves, and fresh weight was inhibited. Our results indicate that mixing three parts PM with one part of P45-1 (PM/P45-1/3:1) or P45-2 (PM/P45-2/3:1) accelerated flowering and increased the number of florets and leaves compared to other mixing ratios of PM and pellets media. The concentrations of phosphorus (P), calcium (Ca), boron (B), iron (Fe), and copper (Cu) in individual growing medium PL, P45-1, and P45-2 were significantly lower than those in PM. The concentration of N was the highest in leaves of plants grown in P45-1 or P45-2 amended media, and the concentrations of P, Ca, and zinc (Zn) in leaves were lower in individual growing medium P45-1 or P45-2 than in PM and PL. The pH of PM/P45-1/3:1 or PM/P45-2/3:1 media was maintained at optimal level (5.8-5.9) and the concentrations of macro- and micro-elements in the media and leaves were considered to be optimal levels. Therefore, mixing three parts PM with one part P45-1(PM:P45-1/3:1) or P45-2 (PM:P45-2/3:1) is recommended for improved growth and flowering in salvia. This suggests that P45-1 or P45-2 can replace PL as an inert material to prepare growing medium.

• 한국유기농업학회지
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• 제8권1호
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• pp.101-109
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• 1999

In order to investigate the possibility of treating the different mixture ratios of apple pomace and nightsoil sludge by vermicomposting was performed and the stability of worm casts, and availability of worm casts as plant growth media was evaluated by the analysis of chemical composition. The results were obtained as follows ; 1. The value of cast production at 100% apple pomace treatment was higher than those of other mixture ratios of apple pomace and nightsoil sludge. 2. The highest values of increasing rate and reproductive efficiency were obtained at mixture ratios of 60% apple pomace with 40% nightsoil sludge. 3. The value of increasing rate at 100% nightsoil sludge treatment was lower that those of other mixture ratios of apple pomace and nightsoil sludge. 4. Organic matter, available inorganic nutrients and stability of worm casts showed high values in all treatments. It means that worm casts are an excellent plant growth media source with a high chemical composition and their stability.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제21권12호
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• pp.768-773
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• 2015

사회적 혼란을 야기하는 이벤트는 발생 직후 어떻게 대응하느냐에 따라 소요되는 비용의 편차가 크다. 이에 따라 비정상적인 이벤트를 탐지하고 의미를 파악하는 연구가 많이 진행되고 있다. 또한 예측 분석에 관한 연구도 많이 수행되고 있다. 그러나 대부분의 연구는 이벤트의 전체적인 미래 경향에 대한 수치 결과를 예측할 뿐, 이벤트가 내포하는 의미에 대한 예측 연구는 미비하다. 이에 따라 본 논문에서는 비정상적인 이벤트가 내포하는 토픽의 조합을 통해 미래에 어떠한 일이 발생할 수 있는지에 대한 시각적 예측 분석 방법을 제안한다. 제안하는 방법은 먼저 트윗에서 실시간으로 비정상 이벤트를 탐지한다. 그 다음 과거 유사한 사례를 탐색한 다음 이벤트와 관련된 토픽들을 추출한다. 마지막으로 사용자는 의미 있는 토픽의 조합을 통해 미래에 어떠한 일이 발생할 수 있을지 분석할 수 있다. 실험은 두 가지 상황에 대한 예측 분석을 수행하였으며, 실험 결과 본 논문에서 제안한 방법의 타당성을 입증하였다.