• Title/Summary/Keyword: maximum cell density

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Characteristics of Barkhausen Noise Properties and Hysteresis Loop on Tensile Stressed Rolled Steels

  • Kikuchi, Hiroaki;Ara, Katsuyuki;Kamada, Yasuhiro;Kobayashi, Satoru
    • Journal of Magnetics
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    • v.16 no.4
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    • pp.427-430
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    • 2011
  • The rolled steels for welded structure applied tensile stress have been examined by means of magnetic Barkhausen noise (MBN) method and of a physical parameter obtained from a hysteresis loop. The behaviors of MBN parameters and coercive force with tensile stress were discussed in relation to microstructure changes. There is no change in MBN parameters and coercive force below yield strength. The coercive force rises rapidly with tensile stress above yield strength. On the other hand, the rms voltage and the peak in averaged rms voltage take a maximum around yield strength and then decreases. The magnetomotive force at peak in the averaged rms voltage shows a minimum around yield strength. These phenomena are attributed to the combined effects of cell texture and dislocation density. In addition, the behaviors of MBN parameters around yield strength may be reflected by the localized changes in strain field due to the formation of dislocation tangles.

The Effect of Alumina Addition on Microstructure and Mechanical Properties of Plasma-Sparayed Ceria Based Electrolyte Coatings (알루미나 첨가가 플라즈마 용사된 세리아계 전해질체 코팅츠의 미세구조 및 기계적 특성에 미치는 영향)

  • 김장엽;유석원;임대순
    • Journal of the Korean Ceramic Society
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    • v.35 no.6
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    • pp.610-618
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    • 1998
  • Alumina were added to ceria based ceramic powders upto 9.7 vol% and composite powders were sprayed by plasma spraying process in order to improve the mechanical properties such as hardness fracture tough-ness and thermal shock resistance. The ceria based coating sprayed without alumina has the typical colum-nar and lamellar structure. Alumina addition has lowered the amount of columnar and lamellar sturcture Added alumina was segreagated in the grain boundary and grain of ceria based crystal accompanied with pore. The maximum value of density and the minimum value of porosity were observed at the sprayed coating with 4.8 vol% alumina. The hardness fracture toughness and thermal shock resistance were increased with alumina addition. The improvement of mechanical properties of plasma sparyed ceria based coatings result-ed from the disapperance of the columnar and lamellar sturcture by addition of alumina.

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Numerical Simulation of Nearshore Tsunami Using a Digital Wave Tank Simulation Technique (디지털 수치수조 기법에 의한 연안 Tsunami의 수치 시뮬레이션)

  • Park, Jong-Chun;Chun, Ho-Hwan
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2003.05a
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    • pp.231-239
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    • 2003
  • A Digital Wave Tank simulation technique based on a finite-difference method and a modified marker-and-cell (MAC) algorithm is applied to investigate the characteristics of nonlinear Tsunami propagations and their interactions with a 2D sloping beach and Ohkushiri island, and to predict maximum wave run-up around the island. The Navier-Stokes (NS) and continuity equation are governed in the computational domain and the boundary values updated at each time step by a finite-difference time-marching scheme in the frame of rectangular coordinate system. The fully nonlinear kinematic free-surface condition is satisfied by the modified marker-density function technique. The Nearshore Tsunami is assumed to be a solitary wave and generated from the numerical wavemaker in the developed Digital Wave Tank. The simulation results are compared with the experiments and other numerical methods based on the shallow-water wave theory.

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Growth and Photoconductive Characteristics of $CdS_{1-x}Se_x$ Thin Films by the Hot Wall Epitaxy

  • Youn, Seuk-Jin;Hong, Kwang-Joon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.07a
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    • pp.349-352
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    • 2004
  • The $CdS_{1-x}Se_x$ thin films were grown on the GaAs(100) wafers by a Hot Wall Epitaxy method(HWE). The temperatures the source and the substrate temperature are $580^{\circ}C\;and\;440^{\circ}C$ respectively. The crystalline structure of thin films was investigated by double crystal X-tay diffraction(DCXD). Hall effect on the sample was measured by the van der Pauw method and studied on the carrier density and mobility dependence on temperature. In order to explore the applicability as a photoconductive cell, we measured the sensitivity($\gamma$), the ratio of photocurrent to darkcurrent(pc/dc), maximum allowable power dissipation(MAPD), spectral response and response time.

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Ecological Characteristics of Some Algal Populations along Environmental Gradients of Zinc (아연환경구배에 의한 녹조류개체군생장의 생태학적 특성)

  • 송승달
    • Journal of Plant Biology
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    • v.20 no.3
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    • pp.119-126
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    • 1977
  • The maximum relative growth rate of algae treated with Zinc was shown as follows: 15, 8, 6, 3 and -5% per day for the rather sensitive Chlorella sp. populations, or 14, 7, 5 and 4% per day for the Pleurococcus sp. populations, and 22, 20, 13, 9 and 7% per day for the more resistant Scenedesmus spinosus populations, respectively for the culture medium with 0, 1, 5, 10 and 20 ppm of Zinc treatment. With mixed cultures of Chlorella sp. and Scenedesmus spinosus populations, the growth of the Chlorella sp. population overcame that of the S. spinomsus population from the cultures treated with relatively low concentration of Zinc. On the contrary, the population growth of the latter resistant species overcame that of the former sensitive species when the concentration of Zinc was above 5 ppm Zn of the medium. This paper describes the results of further investigations of the effects evaluated by direct cell counts method, optical density comparisons, oxygen production and consumption determinations and the measurements of the fate of Zinc treated in the solutions.

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Study on Development of Horticultural Media Using Macroporous Calcium-Silicate Mineral (다공성규산칼슘계 화합물을 이용한 원예용 상토개발에 관한 연구)

  • Lee, Jong-Jin;Chang, Ki-Woon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.2
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    • pp.101-107
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    • 2001
  • The experiment was conducted to investigate the agricultural utility of macro-porous calcium-silicate rnineral(CellCaSi) as topsoil mixture, as well as to estimate as a soil conditioner. The bulk density of CellCaSi which is consisted of various particle sizes ranging from 1mm to 3.35mm was about $0.42g/cm^3$, and its maximum porosity was approximately 81.4%. We also investigate gerrnination rates for Cabbage and Lettuce to obtain the suitable mixing ratios of CellCaSi with topsoil. Among 4 different mixing ratios, the germination rates of ropsoil mixed with 10% of CellCaSi were 94.1% and 64.6% for Cabbage and Lettuce, respectively resulted in the mosr suitable for germination. The growth rates for Cabbage and Lettuce showed thar 10% and 20% of CellCaSi treatments signification influenced the fresh weight. To observe the adsorption capacity of CellCaSi, CellCaSi was treated with a chemical fertlizer(N:P:K=18:18:18). lncreasing the contents of N, P and K, the amounts of adsorption by CellCaSi for these element also increased. The most suitable types nutrient resources for growth condition of Cabbage, and Lettuce were Fer-1 and Fer-0.5.

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Optimization and Mathematical Modeling of the Transtubular Bioreactor for the Production of Monoclonal Antibodies from a Hybridoma Cell Line

  • Halberstadt, Craig R.;Palsson, Bernhanrd O.;Midgley, A.Rees;Curl, Rane L.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.3
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    • pp.163-170
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    • 2002
  • This report describes the use of a transtubular bioreactor to study the relative effects of diffusion versus perfusion of medium on antibody production by a hybridoma cell line. The study was performed with a high-density cell culture maintained in a serum-free, low-protein medium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence time distribution similar to a continuous stirred tank reactor (CSTR), However, due to the arrangement of the medium lines in the reactor, the flow patterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cyclic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accessible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a lapped volume, which provides nutrients through unilateral convection; and a swept volume, which operates through bilateral convection. The relative sizes of these three volumes were modified experimentally by changing the period over which the direction of medium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The results suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difference in cellular metabolism occurred as the reactor was moved between these different states. These results indicate that the mode of operation of the transtubular bioreactor may influence antibody productivity under serum-free, low-protein conditions with minimal effects on cellular metabolism.

Fed-Batch Culture of Brevibacterium CHI for the Production of Nitrile Hydratase (Brevibacterium CH1의 유가 배양에 의한 Nitrile Hydratase의 생산)

  • 황준식;황영보;이처영;장호남
    • Microbiology and Biotechnology Letters
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    • v.20 no.5
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    • pp.614-618
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    • 1992
  • The batch and fed-batch cultivations of Brevibacteriurn CHI were carried out for the production of nitrile hydratase. In batch culture, with pH control the cell mass and the specific activity increased more 20% and 30%. respectively. The maximum growth rate was obtained at a glucose concentration of $20g/{\ell}$ because of substrate inhibition. The fed-batch culture of Brevibacteriurn CHI with constant substrate feeding gave a cell density of up to $68g/{\ell}$ and nitrile hydratase activity was maintained at above 6.1units/mg. The cell growth yield on carbon .source was ca. 0,68 g/g glucose consumed. The total nitrile hydratase activity in this fed-batch mode increased up to 414.8 units/m${\ell}$, which amounted to 4.4 times that of the batch culture.

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담배세초현탁배양을 이용한 human granulocyte-macrophage colony stimulating factor의 생산에서 배지 성분이 미치는 영향

  • Lee, Gi-Yong;Lee, Sang-Yun;Myeong, Hyeon-Jong;No, Yun-Suk;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.325-328
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    • 2002
  • Production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by Nicotiana tabacum cell suspension culture was studied in Murashige and Skoog (MS) medium with sucrose as a carbon source, ammonium nitrate and potassium nitrate as nitrogen sources, potassium dihydrogen phosphate and sodium dihydrogen phosphate hydrate as phosphate sources, respectively. Optimum concentrations for carbon, nitrogen, phosphate was determined to enhance the production of hGM-CSF. Cell growth was better at high initial sucrose concentration (60 g/L), high initial nitrogen concentration (121.04 mM). Maximum cell density (18.28 g/L) was obtained at 60 g/L of sucrose after 14 days. Cell growth was not so good at low initial sucrose concentration 00 g/L), but the highest hGM-CSF production was obtained at the latter half of exponential phase. hGM-CSF production increased about 3 fold at initial phosphate concentration of 4.96 nM

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Preparation of Enzyme Electrodes for Biofuel Cells Based on the Immobilization of Glucose Oxidase in Polyion Complex (폴리이온복합체를 이용하여 글루코스 산화효소를 고정화한 바이오전지용 효소전극 제조)

  • Nguyen, Linh Thi My;Li, Nan;Yoon, Hyon Hee
    • Applied Chemistry for Engineering
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    • v.24 no.1
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    • pp.99-103
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    • 2013
  • An emzymatic bioanode for a glucose/oxygen biofuel cell was prepared by the sequential coating of carbon nanotube (CNT), charge transfer complex (CTC) based on tetracyanoquinodimethane (TCNQ) and tetrathiafulvalene (TTF), glucose oxidase (GOx), and polyion complex (mixture of poly-L-lysine hydrobromide and poly (sodium 4-styrenesulfonate)) on a glassy carbon electrode. A biocathode was also prepared by the sequential coating of CNT, bilirubin oxidase (BOD), 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and polyion complex. The effect of CNT and CTC on the electrochemical performance was investigated. The biofuel cell exhibited a promising performance with maximum power densities of 3.6, 10.1, and $46.5{\mu}W/cm^2$ at 5, 20, and 200 mM of glucose concentration, respectively. The result indicates that the biofuel cell architecture prepared in this study can be used in the development of biofuel cells and biosensors.