• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.65-72
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• 1994

Purification and characterization of pectate lyase from Bacillus sp HSA-925. Bacillus sp. HSA-925 isolated from soil produced constitutively an extracellular pectate lyase when cultivated in LB broth. The pectate lyase(EC 4.2.2.2) was purified from the cuylture broth by preciptation with ammonium sulfate, followed by column chromatography on CM-cellulose C-50 and repeated gel filtration on Sephadex G-75G. The enzyme had a molecular weight of 32-33 kDa. The activity was mazimum at pH 9.5 AND 45$\CIRC $C. The enzume activity was stable at 55$\circ $C for 15 min and between pH7-12. The activation energy, Km and V$_{max}$ for the pectate lyase were 5.8779 kcal/mol, 6.33$\times $10$^{-2}$ mol/ml and 2.09$\times $10$^{2}$ $\mu $mol/min respectively. The enzyme was activated by Ca$^{2+}$, Cu$^{2+}$ and inhibited by Li$^{+}$, Hg$^{2+}$, EDTA.

• Journal of the Korean Mathematical Society
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• v.34 no.2
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• pp.293-307
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• 1997

In this paper we present the $L^2$-estimation for the kernel $K_n$ of the remaider term for the Gaussian quadrature with respect to one of four Chebyshev weight functions and the error bound of the type on the contour $$ $\mid$R_n(f)$\mid$ \leq \frac{2\pi}{\sqrt{l(\Gamma)}} max_{z\in\Gamma}$\mid$f(z)$\mid$ (\smallint_\Gamma $\mid$K_n(z)$\mid$^2$\mid$dz$\mid$)^\frac{2}{1}, $$ where $l(\Gamma)$ denotes the length of the contour $\Gamma$.

• Journal of Environmental Science International
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• v.11 no.9
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• pp.993-999
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• 2002

Waste paper cup was sulfonated to be used as ion exchanger. Removal characteristic of copper and lead ion by prepared ion exchanger was investigated. The sulfonation was conformed by the high intensity band of $SO_3H$ group around 1100~$1160cm^{-1}$. The synthesized ion exchanger had greater removal ability for copper and lead ion than the original waste paper cup. Ion exchange system reached the final equilibrium plateau within 30min. The maximum removal capacities $q_{max}$ were calculated as 9.79mg/g fur copper and 15.95mg/g for lead, respectively. The affinity of lead based on a weight was higher than that of copper. The ion exchange phenomena appeared to follow a typical Freundlich isotherm.

• Tunnel and Underground Space
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• v.5 no.2
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• pp.89-94
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• 1995

Regression analysis and a comparative study were carried out for 52 blast vibration data which were monitored by changing the monitoring distance and charge per delay. The results are as follows: 1) The square and cube root scalings and general equation which have a confidence level at the point of 90% and 99% are V90=33300(SD)-2.026 , V90=23600(SD)-1.993, V90=26300W0.755 R-2.007 and V99=48400(SD)-2.026, V99=34000(SD)-1.993 , V99=38100W0.755R-2.007, respectively. 2) There is need to decide the allowable max. charge weight per delay considering the cross points comparatively of the nomogram constructed using several predictived equations. 3) It is necessary to derive the predictive equation on the basis of blast vibration level monitored in field and to decide safe vibration level and the confidence level.

• Textile Coloration and Finishing
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• v.8 no.1
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• pp.26-33
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• 1996

The berberine from Amur cork tree is natural yellow dye and can be extracted more easily by water. The berberine is basic dye and was extracted at 90-95$^{\circ}C$. We have obtained the following results; 1. K/S value in the abstract of dye was increased by time-elapsing, but the reasonable time was between 90-120min. 2. The reasonable weight of Amur cork tree in the abstract of dye was between 10-20 g/L. 3. We obtained various colors in case of using mordants. ―In case of 10g/L and 20g/l, the reasonable value of mordant was 5-10%(owf). and in case of using tannic acid and $FeSO_{4}$ we could obtain high K/S value. 4. &{\lambda}_{max}& of UV spectra was shown 420nm. 5. Genarally fastness of drycleaning indicated more than grade 4. but it of perspiration was more stable in acid than alkali.

• Journal of Plant Biology
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• v.22 no.1_2
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• pp.1-4
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• 1979

Wild soybean plants(Glycine ussuriensis) collected from Korea(47 strains) and abroad(41 strains) were grown under field condition and classified according to the growth habit. Seeds were analyzed for protein content. The results were as follows: 1. Wild soybean plants were classified into three groups each by seed size, growth habit of stem, leaf size and flowering time. 2. Seed protein was higher in the strains with large leaves, tendril, stem or late flowering. 3. Glycine gracilis, supposed to be the intermediate species between cultivated and wild soybean(according to the stem growth habit, straight or semitendril, and seed size) was medium in protein content of seeds. 4. The average protein content of Glycine ussuriensis, 43.2%, was highest in comparison with those of others such as G. gracilis, 37.5%, or G. max, 36.2%. 5. One hundred seed weight of Amphicarpaea trisperm, similar to the wild soybean, was almost same as Glycine ussuriensis, but protein content of this species was lower than wild soybeans.

• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.439-443
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• 1991

A strain of Streptomyces sp. isolated from soil was found to produce extra-cellular $\beta$-lactamase associated partially to the cell growth. The $\beta$-lactamase was purified from the culture supernatant through anmonium sulfate fractionation, ion-exchange chromatographies and gel filtration. The final purification fold and recovery yield were 57 and 6.2%, respectively. Molecular weight of the $\beta$-lactamase was estimated to be about 67, 000 by SDS-polyacrylamide gel electrophoresis. The optimal reaction condition was at pH 7-8 and at 35-$45^{\circ}C$. The $K_m$ and $V_{max}$ values of the enzyme for penicillin G were estimated to he 3 mM and $3\times 10^3$ $\mu\textrm{M}$/min/mg protein, respectively. The purified $\beta$-lactamase was classified to the class A enzyme hydrolyzing only penicillin.

• Proceedings of the IEEK Conference
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• 2002.06a
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• pp.81-84
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• 2002

The WRR scheduling algorithm is widely used in ATM networks due to its simplicity and the low cost of hardware implementation. It guarantees minimum cell rate according to the weight of each queue. The fairness is a important factor for ABR service. That is, Scheduling algorithm allocates network resources fairly to each VC. In this paper, we propose a scheduling algorithm for satisfying the two fairness criteria, MCR plus equal share and Maximum of MCR or Max-Min share, among the six criteria defined by ATM Forum TM 4.1 specification. The WRR, Nabeshima et al, and the proposed scheduling algorithms are compared with respect to fairness and convergence time throughout experimental simulation.

• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.386-390
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• 1997

A xylanase from the Bacillus sp. strain DSNC 101, isolated from soil, was purified to homogeneity by anion-exchange and hydrophobic interaction chromatography followed by gel filtration chromatography. The enzyme cleaved xylan, but not carboxymethyl cellulose, Avicel, soluble starch, and pNPX. The main product of oat spelts xylan hydrolysates was xylobiose. The xylanase had a molecular weight of 25 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature and pH for the xylanase activity were $50^{\circ}C$ and 6.0, respectively. $K_{m}\;and\;V_{max}$ of the enzyme for oat spelts xylan were 12.5 mg of xylan/ml and 869.5 unit/mg of protein, respectively. Xylanase was completely inhibited by Hg, Cu, and N-bromosuccinimide, but was stimulated by Ca, Co, and Mg.

• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1436-1438
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• 2003

In this paper thermal and mechanical properties of elastic epoxy for applying high voltage products were investigated. Glass transition temperature(Tg) of elastic epoxies can't find form room temperature to $200^{\circ}$ by DSC. It occurred weight reduce on $285^{\circ}$ and $451^{\circ}$ by thermo-gravimeter. The first temperature was effected on addictives and the other was epoxy's character. Max, tensile strain showed $28.3kgf/cm^2$ at 20% of mechanical stress in addictives 35phr. SEM micrograph of the fracture surface observed in void and tearing of elastic epoxy at addictives 35phr. The other side, SEM micrograph of rigid epoxy showed the trace which broke.