• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.854-857
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• 2011

POMT7, which is an O-methyltransferase from poplar, transfers a methyl group to several flavonoids that contain a 7-hydroxyl group. POMT7 has been shown to have a higher affinity toward quercetin, and the reaction product rhamnetin has been shown to inhibit the formation of beta-amyloid. Thus, rhamnetin holds great promise for use in therapeutic applications; however, methods for mass production of this compound are not currently available. In this study, quercetin was biotransformed into rhamnetin using Escherichia coli expressing POMT7, with the goal of developing an approach for mass production of rhamnetin. In order to maximize the production of rhamnetin, POMT7 was subcloned into four different E. coli expression vectors, each of which was maintained in E. coli with a different copy number, and the best expression vector was selected. In addition, the S-adenosylmethionine biosynthesis pathway was engineered for optimal cofactor production. Through the combination of optimized POMT7 expression and cofactor production, the production of rhamnetin was increased up to 111 mg/l, which is approximately 2-fold higher compared with the E. coli strain containing only POMT7.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.17 no.6
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• pp.696-707
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• 1988

This study simulates a typical solar system using the transient simulation program TRNSYS, and calculates the maximum cooling load of the model room of $50m^2$. In this study, energy rate control method is used in calculating a maximum cooling load. On the ground of the maximum cooling load of the model room, the variables that have an effect on the solar collection performance of the solar system are made a selection. Also in this study the trend of the solar collection performance is shown as the variables change. The results show that the variables which have an effect on the collection performance are collector area, collector mass flow rate, collector slope and the volume of storage tank, and the optimal value of Ac/Vt is not constant but varies as the collector area and the collector mass flow rate. Also the results show that for cooling system the optimal value of the collector slope is latitude minus $15^{\circ}$ during the seasonal operations, and twenty percent of the maximum cooling load is saved with the aid of the solar energy.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.41-45
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• 2009

The small-sized bi-propellant thruster using a high concentrated hydrogen peroxide and kerosene as the oxidizer and fuel was designed and fabricated in this study. The water cold-flow test was performed to verify the performance characteristics of the injector. The mixing head assembly used in this model thruster was designed as a structure to combine igniter, injectors and film cooling, which are capable of regulating each mass flowrate. This maximize the experimental verification and efficiency of the design optimization. Finally, the mass flowrate and spray pattern of injector were evaluated by the hydraulic test. Therefore, the design validity of the mixing head was verified.

• ALGAE
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• v.21 no.1
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• pp.133-139
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• 2006

Outdoor cultivation of cyanobacterium Spirulina platensis was carried out for 40 days in a batch mode. A half concentration of the SOT based on the underground water was used as culture medium. Working volume was 5.7 tons with 0.2 m depth. During cultivation, mean water temperature, DO and light intensity were all in proper conditions for the S. platensis growth. The adjustment of pH to over 10 with Na2CO3 and addition of the 1.5% natural salt were very effective to delete contaminant organisms, Chlamydomonas moewusii and Chlorella minutissima occurred one after the other in the culture. The mean productivity of the biomass based on the dry cell weight from 14 to 25 days, after the contaminants were deleted, was 7.8 g ·m–2· d–1, which was relatively high productivity in that a half concentration of the SOT was used for the culture. Underground water used in the culture minimized contaminants invasion and addition of the 1.5% natural salt was effective to delete contaminants as well as acted as mineral supplement in outdoor cultivation of S. platensis. Harvesting using the floating activity of S. platensis was effective from mass floating in day time after overnight without agitation and illumination.

• Geomechanics and Engineering
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• v.15 no.1
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• pp.621-630
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• 2018

In the note a comprehensive and optimal passive-active mode for describing the limit failure of circular shallow tunnel with settlement is put forward to predict the catastrophic stability during the geotechnical construction. Since the surrounding soil mass around tunnel roof is not homogeneous, with tools of variation calculus, several different curve functions which depict several failure shapes in different soil layers are obtained using virtual work formulae. By making reference to the simple-form of Power-law failure criteria based on numerous experiments, a numerical procedure with consideration of combination of upper bound theorem and stochastic medium theory is applied to the optimal analysis of shallow-buried tunnel failure. With help of functional catastrophe theory, this work presented a more accurate and optimal failure profile compared with previous work. Lastly the note discusses different effects of parameters in new yield rule and soil mechanical coefficients on failure mechanisms. The scope of failure block becomes smaller with increase of the parameter A and the range of failure soil mass tends to decrease with decrease of unit weight of the soil and tunnel radius, which verifies the geomechanics and practical case in engineering.

• Journal of computational fluids engineering
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• v.20 no.4
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• pp.7-13
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• 2015

Vortex tube is a thermal static device that separates compressed air into hot and cold streams. In general, the cooling efficiency of vortex tubes is lower than that of traditional air conditioning equipment and vortex tubes are mainly used for industrial spot cooling applications because of their quick responses. In this study, conical frustums are employed in the nozzle chamber to improve the cooling performance. Conical frustums can be used to decrease the ineffective mass fraction that directly passes through the cold exit without energy separation. The shape optimization of conical frustums has been performed using full factorial design. It is found that the height of frustums has the largest main effects on the cooling performance. Computational results show that the cooling performance can be increased by about 10% within the considered range of the design parameters. This is because the ineffective mass fraction toward the cold exit is decreased by about 20%.

• Geomechanics and Engineering
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• v.14 no.1
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• pp.91-98
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• 2018

Length of a vehicle is an important variation to generate different variants of an automotive platform. This parameter is usually adjusted by embedding dimensional flexibility into different components of the Body in White (BIW) including the floor pan. Due to future uncertainties, it is not necessarily possible to define certain values of wheelbase for the future products of a platform. This work is performed to add flexibility into the design process of a length-variable floor pan. By means of this analysis, the cost and time consuming process of optimization is not necessary to be performed for designing the different variants of a product family. Stiffness and mass of the floor pan are two important functional requirements of this component which directly affect the occupant comfort, dynamic characteristics, fuel economy and environmental protection of the vehicle. A combination of Genetic algorithm, GMDH-type of artificial neural networks and TOPSIS methods is used to optimally design the floor pan associated with arbitrary length of the variant in the defined system range. The correlation between the optimal results shows that for a constant mass of the floor pan, the first natural frequency decreases by increasing the length of this component.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.20 no.2
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• pp.121-128
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• 2011

As high precision industries such as semiconductor, TFT-LCD manufacturing and MEMS continue to grow, the demand for higher DOF precision stages has been increasing. In general, the stages should accommodate a prescribed range of payloads in order to position various precision manufacturing/inspection instruments. Therefore a nonlinear controller using sliding motion is developed, which bears mass perturbation and makes the upper plate of the stage move in 6 DOF. For the application of the nonlinear control, an observer is also developed based on expected noise covariance. To eliminate the steady state error of step response, integral terms are inserted into the state-space model. The linear term of the controller is designed using optimization scheme in which parameters can be weighted according to their physical significance, whereas the nonlinear term of the controller is designed using trial and error method. A comprehensive simulation study proves that the designed controller is robust against mass perturbation and completely eliminates steady state errors.

• Mass Spectrometry Letters
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• v.11 no.1
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• pp.1-5
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• 2020

In this study, some of the recently reported data processing strategies were evaluated and modified based on their capabilities and a brief workflow for data mining was redefined for Q-TOF LC-MS based untargeted metabolomics. Commercial pooled human plasma samples were used for this purpose. An ultrafiltration procedure was applied on sample preparation. Sample set was analyzed through Q-TOF LC/MS. A C18 column (Agilent Zorbax 1.8 µM, 50 × 2.1 mm) was used for chromatographic separation. Raw chromatograms were processed using XCMS - R programming language edition and Isotopologue Parameter Optimization (IPO) was used to optimize XCMS parameters. The raw XCMS table was processed using MS Excel to find reliable and reproducible peaks. Totally 1650 reliable and reproducible potential metabolite peaks were found based on the data processing procedures given in this paper. The redefined dataset was upload into MetaboAnalyst platform and the identified metabolites were matched with 86 metabolic pathways. Thus, two list were obtained and presented in this study as supplement files. The first list is to present the retention times and m/z values of detected metabolite peaks. The second list is the metabolic pathways related with the identified metabolites. The briefly described data processing strategies and dataset presented in this study could be beneficial for the researchers working on untargeted metabolomics for processing their data and validating their results.

• Aerospace Engineering and Technology
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• v.10 no.1
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• pp.1-12
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• 2011

The error of onboard propellants mass which is mostly occupied in total mass of launch vehicle and The error of residual affect the performance of launch vehicle seriously. In other words, the errors directly cause the error of total impulse. Therefore, optimization of performance of launch vehicle can be achieved by the minimization of the residual. For minimizing the residuals, the active control for completely depleting the propellants and the calculation method using probability for minimizing the residuals have been researched. In this paper, the added fuel was calculated for minimizing the residual and the minimized residual was predicted by the presented method.