• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.79-81
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• 1982

Cytoplasmic male- sterile Hyangchio, derived from male- sterile Burley 21 containing N. megalosiphon cycloplasm, and male- fertile Hyangchio (Korean local aromatic variety) were evaluated in replicated pot trials in green-house. Male- sterile Hyangchio was comparable to the male- fertile Hyangchio for six agronomic traits and total alkaloids content. The only significant difference was that the male- sterile Hyangchio flowered 2.3 days later than the male- fertile Hyangchio. M ale- sterile Hyangchio has increased the yield than the male- fertile Hyangchio significantly in case of non- topped.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.74-77
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• 1986

The male sterility gene of IR36ms was transferred to four Korean rice cultivars Gayabyeo, Nampungbyeo Sinkwangbyeo and Suweon 296 by five times back crosses. From the BC$_4$F$_2$, the genetic male sterile rices having the backgrounds of the Krean cultivars, Gayabyeo ms, Nampungbyeo ms, Sinkwangbyeo ms and Suweon 296 ms were selected. No differences in number of panicles per hill, number of florets per panicle, heading date and length of panicle, were found between the male sterile lines and their parental cultivars in the four series of male sterile rices. The culm length of the male sterile lines was shorter than that of their parental cultivars in the four male sterile rice. Significant difference in out cross rate was found from the genetic male sterile rices having different back-grounds even though they have the same male sterility gene. The out cross rates of Gayabyeo ms, Nampungbyeo ms, Sinkwangbyeo ms and Suweon 296 ms were 11.5%, 13.1 %, 1.9% and 12.7% respectively. No difference in out cross rate was found on the genetic male sterile plants planted from I 5cm to 90cm from the pollinater.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.298-301
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• 2010

A new intermediate parent 'Wonye 30002' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30002' can be used to hybrid seed production by crossing with pollen parent. The first cross was conducted between male sterile plants of 402AC203 and M1 in 2002. The male sterile line 'Wonye 30002' has circular bulb and bulb weight is 283 g. As early maturing type, lodging date is May 6. Plant height and pseudostem diameter are 43 cm and 15.5 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 43 cm and 110 cm, respectively. The flowering date of 'Wonye 30002' is around May 24 and is completely male sterile. 'Wonye 30002' is a promising male sterile line for hybrid bulb onion seed production.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.212-216
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• 1998

Capsicum pepper selections fro PI163192, PI241670, PI244670, PI271322, PI308787, PI322719, and PI369994 were confirmed to be non-hypersensitively resistant to race 3 of Xanthomonas campestris pv. vesicatoria. A resistant cultivar,‘SR’, was shown to be hypersensitive. Four Koran local cultivars, a cytoplasmic male sterile line (A-line) and its maintainer (B-line) were highly susceptible. The resistant selections and cultivars were crossed with a male sterile A-line (Smsms) and fertility of their F1 hybrids was examined by observing he pollen production, testing pollen germination, and quantifying the amount of pollen produced per anther to identify the genotype interacting with the male sterile cytoplasm. The seven resistant PI selections turned out to be restorers (N(S)MsMs) and‘SR’to be a maintainer (Nmsms).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.427-430
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• 1985

Extrusion degree of stigma, numbers of opened florets at different time during anthesis, angle of opening florets and outcrossed seed set percent were investigated in four male sterile rices having background of Korean cultivars; Suwon 296A, Suwon 304A, Suwon 310A and Suwon 296GMS, and the Chinese cytoplasmic genetic male sterile rice Zhenshan 97A as control in order to get basic informations for hybrid rice breeding. The results obtained were summarized as follows; Extrusion degree of stigma in the four Korean male sterile lines tested was lower than in the Chinese one Zhenshan 97A. The better extrusion of stigma showed the higher outcross rate in male sterile rices. There were almost no relationships between peak of anthesis and outcrossed seed set in the male sterile rices tested. Large anthesis angle with long anthesis duration appeared to be essential for high outcrossed seed set of the male sterile rices. The genetic male sterile line showed the larger anthesis angle than the cytoplasmic and genetic male sterile one in the lines having the same background to the Korean rice cultivar Suwon 296.

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.364-371
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• 2017

The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.

• Horticultural Science & Technology
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• v.29 no.1
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• pp.53-60
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• 2011

Cytoplasmic male sterility (CMS) induced by mutant mitochondria genome, has been used for commercial seed production of $F_1$ hybrid cultivars in diverse crops. In pepper (Capsicum annuum L.), two sterile cytoplasm specific gene organization, atp6-2 and coxII were identified. An open reading frame, orf456 nearby coxII gene has been speculated to induce male sterility (MS) by mutagenic analysis. Moreover, molecular markers for atp6-2 and coxII of mitochondrial genotype (mitotype) were developed. However, the Cytoplasmic MS specific markers, atp6SCAR and coxIISCAR markers appeared in both N and S cytoplasms when polymerase chain reaction (PCR) cycles prolonged more than 40 cycles. Since the reported molecular markers were dominant markers, the presence of the faint sterile-specific band in normal cytoplasm may lead to the mis-classification of pepper breeding lines. To solve this problem, one common forward primer and two different reverse primers specific to normal coxII and sterile orf456 genes were designed after analyzing their gene organizations. By using these three primers, N and S coxII specific bands were co-amplified in male-sterile lines, but only normal coxII specific band was amplified in maintainer lines. Since the reverse primer for sterile coxII was specifically designed 275 bp downstream of orf456, relatively stable PCR amplification patterns were observed regardless of the number of PCR cycles. These primer sets easily identified different mitotypes among the divergent breeding lines, commercial cultivars and diverse germplasms.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.294-297
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• 2010

A new intermediate parent 'Wonye 30001' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30001' could be used for hybrid seed production by crossing with pollen parent. The first cross was conducted in 2000. Male sterile plants were selected from 'Ginque' breeding lines and fertile plants were selected from YG-1-1. The male sterile line 'Wonye 30001' has circular bulb and bulb weight of 260 g. As mid-late maturing type, lodging date is around May 20. Plant height and stem diameter are 57 cm and 15.9 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 6 and 135cm, respectively. Flowering date of 'Wonye' is May 23 and is completely male sterile. 'Wonye 30001' is a promising male sterile line for hybrid bulb onion seed production.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.239-246
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• 2010

The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ($^{60}Co$) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from $M_1$ generation at the doses (10 to 50 Gy). We also found that some $M_1$ plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using $KI-I_2$ solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutations like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.73-78
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• 2012

KC00256, KC00406, KC00462, KC00463, KC00820, and KC00821, the genetic resources that have previously been reported as moderately resistant to Phytophthora capsici, as well as the line KC01322, a new source of moderate resistance introduced from Laos, were tested against two strains (Pc003 and Pc005) of P. capsici. We also determined the nuclear restorer genotypes of these lines, in regards to their interaction with cytoplasmic male sterility, through crossing the resources with cytoplasmic male sterile Punggok-A (Srfrf) and determining the fertility of the $F_1$ hybrids. The studied lines exhibited a low level of resistance to both the strains of P. capsici compared to highly resistant CM334, but their response was fairly consistent for both P. capsici strains. KC00406, KC00462, KC00463, and KC01322 produced stable, male fertile $F_1$ plants indicating that they are restorers with genotype N(S)RfRf. KC00821 produced male sterile $F_1$ plants and was identified as a maintainer with genotype Nrfrf. The $F_1$ plants of the KC00820 cross, however, set a few male fertile flowers in the greenhouse at seedling stage, then became male sterile after being transplanted to the plastic greenhouse soil in May and remained so to the end of the growing season. Therefore, KC00820 is an unstable maintainer with genotype Nrfrf. The moderate resistance exhibited by these genetic resources may be integrated into breeding programs aimed at promoting higher levels resistance via recurrent selection or hybridization.