The purpose of this study was to investigate the relationship between percieved parenting by high school students and drinking, smoking and substance use of adolescents. The subject were 257 adolescents of 2nd grade from high school in Taejon (male 126, female 131). The questionnaires consist of 37 items. It was used for adolescents. The respondents were asked complete the self reported questionnaire and the data were analyzed with reliability, frequencies, percentage, means, standard deviation, Pearson correlation coefficient and t-test. The major findings are as follows. 1. Forty-six percent of the boys and 19 percent of the girls have been drinking, 15.8 percent of boys 1.5 percent of girls have been smoking, substance use were 1.6 percent of all. 2. Boys did more drinking, smoking and substance use than girls did. 3. Adolescent's drinking, smoking and substance use behavior were associated with controlled and rejected parenting perceived by adolescents. 4. Parent's socio economic status was not associated with adolescent's problem behavior.
Jang Won-Seok;Yang Jae-Won;Joung Yoo-Sook;Hong Sung-Do
Journal of the Korean Academy of Child and Adolescent Psychiatry
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v.17
no.1
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pp.3-9
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2006
Objectives: Obesity is one of risk factors for psychological and social adjustment problems (depression, low self esteem, low school function etc). Quality of life(QOL) is comprehensive and multidimensional construct including physical, emotional, social and school functioning. Some authors reported that child and adolescent obesity is associated with impaired QOL. This study was to examine the association between QOL and body mass index (BMI) in middle school students. Methods : Cross sectional analysis of 471 adolescents between the ages of 13 and 14 years ($mean{\pm}SD,\;13.4{\pm}0.6$), who attended one of middle schools in Seoul, Korea. Participants' QOL were scored by using Korean version of pediatric QOL inventory generic score scale ($PedsQL^{TM}$ 4.0). Participants had a $mean{\pm}SD\;BMI\;of\;21.3{\pm}4.0$. Self reports and parent proxy reports using $PedsQL^{TM}$ 4.0 were administered to measure participant's QOL. Participants were divided into four groups by BMI (Male: obesity group >26.5, at risk group 23.2-26.5, normal weight group 15.8-23.1, underweight group <15.8, Female : obesity group >25.2, at risk group 22.9-25.2, normal weight group 16.0-22.8, underweight group <16.0). Results : Compared with other groups, obese adolescents reported significantly(p<0.01) love. QOL in all domains. Obese adolescents were more likely to have impaired QOL than normal weight adolescents (Odds Ratio, 8.3, 95% confidence interval, 4.0-16.7). Conclusion : Obese adolescents have lower QOL than normal weight adolescents. Psychiatrist, parents, and teachers need to be informed of the risk fur impaired QOL in obese adolescents to target interventions that could enhance health outcomes.
We observed that the growth and physiological change in Haliotis discus discus by low-dose irradiation with gamma ray.Irradiation with gamma ray was undertaken by using the low-level irradiation facility ($^{60}CO$) in cooperation with the Institute of Nuclear Science and Technology at Jeju National University. The parent abalones were attached one by one and then fixed by using the rubber band to the front side of the fabricated case for irradiation with gamma ray. The experimental plots of irradiation with gamma ray were set as 10, 15, 20 and 25 Gy and the 25 female abalones and 10 male abalones were utilized for each experimental plot. The sperms and eggs were fertilized by setting an interval for each dose to prevent mixing with other experimental plots when fertilizing the sperms and eggs for each dose of irradiation with gamma ray. As for the fertility, it was confirmed to be 85% the control and 10 Gy groups, whereas it was found to be 80%, 65% and 50% in the 15 Gy, 20 Gy and 25 Gy groups, respectively. As a conclusion, the hatching rate and attachment rate were higher at 10 and 15 Gy than the other experimental plots, and the growth rate was higher at 20 Gy than the other experimental plots. Also the changes in lysozyme activity in accordance with the stress of water temperature were found to have a significant increase in the other experimental plots as compared with the control plot at the end of 0 h. The changes in lysozyme activity have remained constant in all the experimental plots at the end of 12 h. These results allowed us to confirm that lysozyme was undertaking the biodefense action by reacting sensitively to the stress of water temperature in the control experimental plot. As for the other experimental plots, they are believed to avoid the biodefense mechanism due to the high degree of anti-parasite mechanism and anti-viral mechanism. Thus, it is believed that it would be imperative to conduct development and research on breeds that were potent for environmental tolerance by applying the method of irradiation with gamma ray to other marine animals and plants.
Lee, Seung Cho;Parent, Jean-Sebastien;Ernst, Evan;Berger, Frederic;Grimanelli, Daniel;Martienssen, Robert A.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.20-20
/
2017
Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.
Lee, Seung Cho;Parent, Jean-Sebastien;Ernst, Evan;Berger, Frederic;Grimanelli, Daniel;Martienssen, Robert A.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.97-97
/
2017
Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.
Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as '10H08'. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard's distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line '10H08' were tested with 29 primer pairs based on microsatellites to investigate purity testing of $F_1$ hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity.
Yi, Jae-Seon;Cheong, Eun-Ju;Moon, Heung-Kyu;Dale, Glenn T.;Teasdale, Robert D.
Journal of Forest and Environmental Science
/
v.11
no.1
/
pp.81-101
/
1995
Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of $F_1$ hybrids includes 186 RAPD markers and 16 linkage groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1-determination of species specific markers for genes controlling traits of commercial interest, and stage 2-determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.
Park, Han-Ju;Lee, In-Ho;Park, Jong-In;Yang, Seung-Yul;Nou, Ill-Sup
Korean Journal of Plant Resources
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v.20
no.4
/
pp.298-303
/
2007
To examine taxonomic relationships among 24 leaf mustard allies (Brassica juncea) derived from domestic collections and introduced species, principal component analysis (PCA) and cluster analysis on 16 morphological characters were carried out. Of 16 characters, total fresh weight, total dry weight, shoot fresh weight and leaf length were useful characters for the understanding of taxonomic relationships among them. Cluster analysis using scores of the principal components indicated that 24 leaf mustards could be grouped into domestic collections and introduced species at 1.0 of average distance in UPGMA. Moreover, in experiment of $F_{1}$ recombination test, heterosis appeared greatly in Sanchiohbachirimen takana A ${\times}$ Akaohba takana, Goheung namyang ${\times}$ Sanchiohbachirimen takana B, and Goheung namyang ${\times}$ Akaohba takana cross combinations. Sanchiohbachirimen takana A and Akaohba takana have a high parent combining ability for breeding cultivars using cytoplasmic male sterility.
Journal of the korean academy of Pediatric Dentistry
/
v.42
no.2
/
pp.158-163
/
2015
General anesthesia (GA) for dental care in handicapped patients is necessary to facilitate the provision of safe, efficient, and effective quality treatment. The aims of this study were to determine the anesthetic characteristics of handicapped patients in need of dental treatment in these day care units, and to establish for plan to provide better services. 325 patients who had outpatient general anesthesia from January 2005 to March 2014 were assessed for this study. Patients' distribution and treatment patterns were examined. The proportion of male patients (202, 62.2%) was higher than female patients (123, 37.8%) and the largest group of patients were 5 to 10 years old (85, 26.2%). The reasons for general anesthesia included mental and physical disabilities (207, 63.7%), behavior management (84, 25.8%), parent needs (14, 4.3%), and so on. Restorative treatment was the most common procedure with the average of 4.2 teeth treated per one patient and 43 (13.2%) patients underwent general anesthesia for dental treatment more than once. To expand and improve access to the dental care of the disabled, improvement of the health care system, enhancement of their training for dental care by professionals, and enlarging caregivers'understanding of the importance of oral care in the early stages are required.
The pipefish Syngnathus schelegeli was reared in the laboratory from May to June 1991 and observed the morphology of eggs and larvae squeezed from the parent fish(male). The diameter of inseminated eggs ranged from 0.72 to 1.01 mm (n=50), and yolk in yellow color were found in the eggs. The newly beared larvae were 10.9 mm in average standard length and had 59~60 myomeres. In 6 days after bearing, the post larvae attained 13.8 mm in average standard length and the low jaw was developed. The larvae of 14.1 mm in average standard length (8 days after bearing) had 40~42 fin rays in dorsal fin, 9 in caudal fin and 13~14 in pectoral fin. The juvenile of 14.7 mm in average standard length (10 days after bearing) had the well elongated snout along with the opercular and caudal fin similar to adult stage's.
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