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Study on Measurement of Carcinogen-DNA adducts in exfoliated urothelial cells among workers by 32P-postlabelling methods (근로자의 뇨중 상피세포에서 32P-postlabeling에 의한 발암물질의 DNA adducts측정방법에 대한 연구)

  • Lee, Jin Heon;Roh, Jaehoon;Talaska, Glenn
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.10 no.1
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    • pp.1-17
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    • 2000
  • Carcinogen-DNA adduct analysis has potential for biomonitoring the earliest effects of exposure to many chemical carcinogens. They are the covalent reaction products of electrophiles and nucleophilic sites on DNA and the initial damage to DNA induced by many carcinogens. So many researchers begin to use them as biomarker for monitoring the earliest exposure of carcinogens and develop the effective analytical techniques about them. Randerath, Gupta and coworkers(1981, 1982) has also developed a $^{32}P$-postlabelling method as one among them. A major project for biomonitoring workers with carcinogen-DNA adducts is to develop non-invasive samples instead of tissues of target organs such as baldder and lung. This study use the exfoliated urothelial cells in urine for examine benzidine-DNA adducts. The content of exfoliated urothelial cells is not enough to significantly measure DNA content with spectrophotometer, and require the another way. So firstly washing the collected cells with PBS and 70% ethanol and centrifuge them for removing the crystals in urine, which block the isolation of DNA adducts. And then, measure the total nucleotide after $^{32}P$-postlabelling for calculating RAL. $[{\gamma}-^{32}P]ATP$ using for $^{32}P$-postlabelling, can synthesize with $[^{32}P]H_3PO_4$, and reagent and enzyme mixture (RM, EM), which is very economic in case of requiring a lot of them. Chromatography was composed of two steps. First step was to separate adduct ones from unadducted nucleotide, and secondary step was separate each adduct, which were performed with 4 kinds of solvents and different directions on TLC. With this procedure, we measure the DNA adducts in exfoliated urothelial cells of workers who were employed in benzidine and benzidine-dye company. RAL of adducts were $89.0{\times}10^7$ and $57.0{\times}10^7$ in them. In conclusion, we can significantly measure the DNA adduct in exfoliated urothelial cells by using the above $^{32}P$-postlabelling procedures, and use them to be biomonitoring workers who exposed carcinogens.

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Schematic Maps of Ocean Currents in the Yellow Sea and the East China Sea for Science Textbooks Based on Scientific Knowledge from Oceanic Measurements (관측 기반 과학적 지식에 근거한 과학교과서 황해 및 동중국해 해류모식도)

  • PARK, KYUNG-AE;PARK, JI-EUN;CHOI, BYOUNG-JU;LEE, SANG-HO;SHIN, HONG-RYEOL;LEE, SANG-RYONG;BYUN, DO-SEONG;KANG, BOONSOON;LEE, EUNIL
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.22 no.4
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    • pp.151-171
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    • 2017
  • Most of oceanic current maps in the secondary school science and earth science textbooks have been made on the base of extensive in-situ measurements conducted by Japanese oceanographers during 1930s. According to up-to-date scientific knowledge on the currents in the Yellow Sea and the East China Sea (YES), such maps have significant errors and are likely to cause misconceptions to students, thus new schematic map of ocean currents is needed. The currents in the YES change seasonally due to relatively shallow water depths, complex terrain, winds, and tides. These factors make it difficult to construct a unified ocean current map of the YES. Sixteen major items, such as the flow of the Kuroshio Current into the East China Sea and its northward path, the origin of the Tsushima Warm Current and its path into the Korea Strait, the path of Taiwan Warm Current, the Jeju Warm Current, the runoff pattern of the Yangtze River flow, the routes of the northward Yellow Sea Warm Current, the Chinese Coastal Current, and the West Korea Coastal Current off the west coast of the Korean Peninsula, were selected to produce the schematic current map. Review of previous scientific researches, in-depth discussions through academic conferences, expert discussions, and consultations for three years since 2014 enabled us to produce the final ocean current maps for the YES after many revisions. Considering the complexity of the ocean currents, we made seven ocean current maps: two representative current patterns in summer and winter, seasonal current maps for upper layer and lower layer in summer and winter, and one representative surface current map. It is expected that the representative maps of the YES, connected to the current maps of the East Sea and the Northwest Pacific Ocean, would be widely utilized for diverse purposes in the secondary-school textbooks as well as high-level educational purposes and even for scientific scholarly experts.

A comparison of the perceptual-auditory voice quality evaluation (GRBAS) and voice-related quality of life (K-VRQOL) according to choir type of elderly women choir members (여성 노인 합창단원의 합창단 유형에 따른 청지각적 음성평가(GRBAS) 및 음성관련 삶의 질(K-VRQOL) 비교)

  • Lee, Hyeonjung;Kang, Binna;Kim, Soo Ji
    • Phonetics and Speech Sciences
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    • v.12 no.2
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    • pp.51-61
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    • 2020
  • The purpose of this study is to compare voice characteristics and voice-related quality of life (K-VRQOL) of the elderly female choir members using perceptual-auditory voice quality evaluation (GRBAS) and K-VRQOL scales. The participants were 77 women over 60 years old who were actively engaged in the choir in either Seoul or Busan. There are two kinds of choirs that indicate different engagement levels: regular choir and church choir. The perceptual-auditory vocal quality evaluation was listened to by / a / vowels and were graded by experts using the GRBAS scale. As a result, when comparing the differences between groups, the elderly female participants of the regular choir showed higher satisfaction in speech using the subjective speech recognition level than the elderly female members who performed in the church choir. In addition, the analysis showed that the satisfaction level was high in the physical function area of the K-VRQOL scale. This study confirmed that choral activities could yield positive results not only in terms of improving voice function in old age, but also to improve the subjective perception level of voice use, thus suggesting the necessity of systematic music programs to improve voices that are aging.

Petrology of the Cretaceous Volcanic Rocks in the Hampyeong Area (함평지역 백악기 화산암류에 대한 암석학적 연구)

  • Cho, Dong-Hyun;Yun, Sung-Hyo;Koh, Jeong-Seon
    • The Journal of the Petrological Society of Korea
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    • v.18 no.2
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    • pp.93-114
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    • 2009
  • Lithological and petrochemical characteristics and tectonic setting of the Cretaceous volcanic rocks in Hampyeong area located in the southwestern part of Okchon Zone, were studied by field survey and petrochemistry of major, trace, and rare earth elements. The $SiO_2$contents of the volcanic rocks range from 50.8 to 77.2wt.%. With increasing $SiO_2$, $Al_2O_3$, $Fe_2O_3\;^T$, $TiO_2$, MnO, CaO and MgO contents decrease and $K_2O$content increase, but $Na_2O$content is scatter to the trend. According to TAS and AFM diagrams, the Cretaceous volcanic rocks are calc-alkaline series. On the discrimination diagram of $K_2O$versus $SiO_2$, the volcanic rocks belong to high-K rocks series. The trace element compositions and REE patterns of the volcanic rocks, characterized by a high LILE/HFSE ratio and enrichments in LREE, indicate that they are typical of continental margin arc calc-alkaline volcanic rocks associated with the subduction environment. The ratios of Ba/Ta and Ba/La indicate that they are associated with volcanic arc-related magmatism. The Cretaceous volcanic rocks in Hampyeong area might be located in the Eurasian continental margin, related to the Pacific type tectonic environment during the Cretaceous times.

Research on the Status and Needs of Career Counseling based on the Student's Perception of Specialized Vocational High School in Daejeon (대전광역시 특성화고 학생들의 인식에 터한 진로 상담 운영 실태와 요구조사)

  • Yang, Seon-Ju;Kim, So-Yeon;Lee, Kyu-Nyo
    • 대한공업교육학회지
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    • v.39 no.1
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    • pp.85-101
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    • 2014
  • This study investigated the current status and needs of career counseling based on the student's perception of specialized vocational high school in Daejeon. The main purpose of this study is to provide the future direction and improvement strategies of career counseling in specialized vocational high school through the evaluation of current situation and student's needs. The analysis results of this study are as follows. First, most of students decided their admission into a specialized vocational high school when they were three grades in middle school. It indicates that career counseling teacher plays important roles during that time. Thus, it is necessary to strengthen the ability of career counseling teachers and to develop the various programs to seek student's aptitude and talent from the period of middle school. Thus, it is necessary to strengthen the ability of career counseling teachers and to develop the various programs to seek student's aptitude and talent from the period of middle school. Second, since most of career counseling takes place primarily with class teacher, professional counseling education for class teachers is required. It also needs to expand the specialized counselors to reduce the overburden of class teachers and develop various counseling programs. Third, vocational high school students need the specialized counseling programs according to their major. Effective group counseling programs as well as one-on-one counseling programs which can help the student's career decision should be developed.

Analysis of socio-demographic and dietary factors associated with fruit and vegetable consumption among Korean adolescents: use of data from the 7th and 8th Korea National Health and Nutrition Examination Survey (2016-2019) (한국 청소년의 과일 및 채소 섭취와 관련된 인구사회학적 특성 및 식생활 분석: 국민건강영양조사 제7-8기 (2016-2019) 자료 이용)

  • Bokyeong Yun;Seunghee Kye
    • Journal of Nutrition and Health
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    • v.57 no.3
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    • pp.292-306
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    • 2024
  • Purpose: This study investigated fruit and vegetable intake and associated socio-demographic and dietary factors, and compared the nutritional intake according to the fruit and vegetable intake level among Korean adolescents. Methods: This study was conducted on 1,676 adolescents who participated in the 2016-2019 Korea National Health and Nutrition Examination Survey. The subjects were classified into four groups based on the fruit and vegetable intake recommendations in 2020 Dietary Reference Intakes for Koreans: Application (KDRIs Application): sufficient fruit intake (SF) group, sufficient vegetables intake (SV) group, sufficient fruit and vegetables intake (SFV) group, and not sufficient fruit and vegetable intake (NS) group The nutrient intake per day in each group was compared.. Logistic regression analysis was performed to examine the factors influencing fruit and vegetables intake. Results: In the sample of adolescents surveyed, only 1.40% met the recommended daily intake of fruits and vegetables, while 79.54% fell below the established threshold for adequate consumption. Female adolescents, those with fathers holding university degrees or above, and those who ate breakfast at least three times a week were likelier to have adequate fruit intake. Male adolescents and those from higher-income households were likelier to consume vegetables. Females, those who ate out daily, those from lower-income households, and those who understood food labels were likelier to have adequate fruit and vegetable intake. The daily nutrient intake and intake-to-requirement ratio significantly differed according to the fruit and vegetable intake groups. The NS and SF group had lower ratios for calcium and iron, while the NS group had the lowest vitamin A and C intake. By contrast, the SFV group met almost all daily nutrient requirements, except for calcium and vitamin A. Conclusion: This study highlights the need for nutrition education programs to encourage adolescents to consume adequate amounts of fruits and vegetables.

Daegu metropolitan government's plan of relocation of water intake plant and collaborative governance between regions (대구시 취수원 이전계획과 지역 간 협력적 거버넌스)

  • Choi, Byung-Doo;Chae, Eun-Hye;Yang, Min-Ji
    • Journal of the Korean association of regional geographers
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    • v.22 no.1
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    • pp.64-85
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    • 2016
  • This paper is to consider the development process of conflict between Daegu and Gumi which has been arisen from Daegu city's plan of relocation of water intake plant. It first argues that water is a major common and public resource which is circulated through the social process as well as the natural one, and hence that conflicts within or between regions due to its allocation should be resolved or managed by construction of collaborative governance in which all stake-holders participate. In particular, it is pointed out that collaborative governance would be not only oriented to a normative goal, but also be mobilized as a strategic means. On the basis of this conceptual consideration, this paper explores the development process of conflict caused by Daegu city's plan of relocation of water intake plant, and analyze the starting conditions and deliberation process of the public-private committee which has been constituted through a consensus between Daegu and Gumi city government. It concludes that the current interruption of the committee has happened, because it has been mobilized politically as a strategic means for regional hegemony, not as a normative one to resolve the conflict.

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A Study on Making Better Use of the Paper Map with QR codes - Focused on the Survey about Intending to Use and Providing Information - (QR코드를 이용한 종이지도의 활용도 증대방안 연구 - 종이지도용 QR코드 사용의사 및 정보제공 수요 조사를 중심으로 -)

  • Yi, Mi Sook;Shin, Dong Bin;Hong, Sangki
    • Spatial Information Research
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    • v.20 no.6
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    • pp.77-90
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    • 2012
  • In this paper, we examined how to utilize QR codes for meeting the information demand and making better use of the paper map. By Decision Tree Analysis, we investigated whether to have any intention to use the paper map with QR codes for receiving more information and what decision variables affect the answers. Thus, we also surveyed the area of providing information and sectoral demand for deriving additional information demand to being provided through QR codes. In the results of our study, we confirmed that the decision variables, to make any intention to use the paper map with QR code, are the frequency of using the paper, the experience of using the paper map, the intention to buy the paper map, the experience of using QR codes and the experience of buying the paper map. In these variables, the frequency of using the paper map is a major factor to decide whether it is intended to use the paper map with QR codes. we also identified that there are various additional information demand using the paper map with QR codes in the area of 'Daily life', 'Real estate', 'Education', 'Travel and Leisure', and 'Entertainment'. Especially additional information demand is high in the area of 'Travel and Leisure'. These results could be used to find a way how to vitalize the usage of paper map by introduction of QR codes and how to develop QR codes for the paper map and concerning applications.

A Pychoeducational Group Intervention for Women with Primary Breast Carcinoma (유방암 환자에 대한 심리교육 집단개입의 효과)

  • Hwang, Sook-Yeon;Lee, In-Soo;Park, Byeong-Woo
    • Korean Journal of Social Welfare
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    • v.51
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    • pp.93-118
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    • 2002
  • The objective of this study was to determine the effect of a psycho-educational group intervention in reducing psychologic distress and enhancing coping in Korean breast cancer patients. The patient selection criteria were age younger than 70 years, having any postoperative adjuvant therapy, and surgery undergone within the previous 12 months as of the start of the study and there were 70 patients eligible for this criteria. They were randomized into three groups; experimental group(24 patients), wait-list control group(24 patients) and wait-list group(36 patients). We conducted a 8-week, structured, psychosocial group intervention, which used psycho-educational strategies combining education and psychological support. Subjects were assessed for psychological distress and coping by administering the Beck Depression Inventory(BDI) and the Ways of Coping Checklist-Revised(WCCL-R) at the baseline and after 8 weeks. Forty eight patients were participated and thirty five patients completed the study. The experimental group had significantly lower scores than the controls for depression on the BDI(p=.012) after 8-week intervention. However, coping did not show a significant difference on WCCL-R after the intervention. Despite of some limitations, the results of this study suggest that a short term psychosocial group intervention produces a significant improvement in the quality of life of patients with primary breast carcinoma in Korea in terms of managing depression.

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A study on the weight control behavior according to cluster types of the motivation to use social media among university students in the Jeonbuk area (전북지역 대학생의 소셜미디어 이용동기 유형에 따른 체중조절 행태 연구)

  • Jiyoon Lee;Sung Suk Chung;Jeong Ok Rho
    • Journal of Nutrition and Health
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    • v.56 no.2
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    • pp.203-216
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    • 2023
  • Purpose: This study examines the weight control behavior depending on university students' motives of using social media. Methods: The participants were 447 university students in the Jeonbuk area. Collected data were analyzed using factor analysis, cluster analysis, analysis of variance, and χ2 tests with SPSS v. 26.0. Considering the motives of using social media, we investigated the usage of social media, dietary behavior related to social media, and weight control behavior. Results: Using the K-clustering method, the motives to use social media were categorized into three clusters: cluster 1 was the interest-centered group, cluster 2 was the multipurpose information-seeking group, and cluster 3 was the relationship-centered group. Among the various social media sites, YouTube (86.8%), Instagram (76.1%), and Facebook (61.1%) were the most visited by the subjects. The dietary behavior related to social media in cluster 2 was significantly higher than clusters 1 and 3 (p < 0.001). Clusters 1 and 2 showed a significantly higher dissatisfaction with one's weight (p < 0.05) and consequent interest in weight control than cluster 3 (p < 0.001). Cluster 2 used weight control-related information from social media significantly more than other clusters (p < 0.05). Weight control experiences in cluster 1 and 2 were significantly higher than in cluster 3 (p < 0.001). Conclusion: Differences in dietary behavior related to social media and weight control behavior were observed between cluster types of motivation to use social media. Based on the usage motives of university students and their behaviors, we propose that educational programs should be conducted for weight control using social media.