• 한국자원식물학회지
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• 제27권3호
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• pp.223-228
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• 2014

Magnoflorine, an important compound in Aristolochia, was usually used as an anxiolytic chemical. In this study, the magnoflorine was isolated from Aristolochia and the biological activities such as antioxidant, ${\alpha}$-tyrosinase inhibitory, anti-inflammatory, and anticancer activities were investigated. The magnoflorine showed significant antioxidant activity as a 2,2-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenger, $50{\mu}g/mL$ of the magnoflorine scavenged about 70.8% of all the free radicals. And it was good at ${\alpha}$-tyrosinase inhibiting, $100{\mu}g/mL$ of the magnoflorine inhibited 36.5% of the tyrosinase. High dosage of magnoflorine inhibited the inflammation production nitric oxide (NO), and the magnoflorine protected the murine macrophage cells (RAW 264.7) from LPS-induced apoptosis. The cell viability of human colon cancer calls (HT-29) was around 100% when treated with different dose of magnoflorine, it's suggesting that magnoflorine had no anticancer effect.

• 약학회지
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• 제57권3호
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• pp.161-166
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• 2013

Sinomenium acutum has been used for the treatment of rheumatoid arthritis, arrhythmia, and pain. We evaluated the quality of S. acutum extract by quantitatively analyzing its components such as sinomenine, magnoflorine and syringaresinol with the simultaneous determination method using HPLC-DAD. A total of 53 samples collected from different localities were evaluated with this quality evaluation method. Sinomenine, magnoflorine and syringaresinol from tested samples ranged from 0.0649~9.1050%, 0.7460~10.7590% and 0.0010~0.2441%, respectively. In the current study, we were able to exhibit the diverse quality of S. acutum samples collected from various locations using the simultaneous determination method.

• 한국식품과학회지
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• 제28권4호
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• pp.616-623
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• 1996

음양곽 성분 중에서 icariin, magnoflorine을 분리하고 이를 표준품으로 음양곽 추출액의 이들 성분에 대하여 HPLC를 이용한 정량법을 수립하였으며 이 성분들의 추출효율을 여러 가지 추출조건하에서 검토하고 그 함량을 분석 하였다. Icariin은 0.1-0.4 mg/ml 농도에서, magnoflorine은 0.02-0.1 mg/ml 농도에서 아래와 같은 조건으로 정량 분석하였다. Icariin의 HPLC조건 :칼럼, LiChrosorbRPl8; 용출용매, MeOH/water/acetic acid (60 : 40 : 0.5); 검출기, 자외부 흡광광도계; 측정파장, 270 nm; 주사량, $25\;{\mu}l$. Icariin의 retention time은 7.37 min으로 다른 성분과도 잘 분리되었다. Icariin 0.1-0.4 mg/ml 50% (v/v)에탄올 용액에 대해 검량선을 작성하여(n=8) 직선을 얻었으며 그 회귀선은 y = -1.4+18296x이고 correlation coefficient (r)=0.9999로 1.00에 매우 접근하였다. Magnoflorine의 HPLC 조건 : 칼럼, LiChrosorb RP18; 용출용매, MeOH/80 mM $Na_{2}\;HPO_{4}/water$ (35 : 35 : 30); 검출기, 자외부 흡광광도계; 측정파장, 270nm ; 주사량, $25\;{\mu}l$. Magnoflorine의 retention time은 11 min이고 $2{\sim}10\;mg/100\;ml$ 50% 에탄올용액을 표준액으로 검량선을 작성하여 직선상의 검량선을 얻었으며(n=6) 그 회귀직선은 y = -0.02+5.7X이고 correlation coefficient (r)=1.0079로 1.00에 근접하였다. 음양곽의 추출용매로 물 또는 50% (v/v)에탄올을 사용하고 추출온도, 추출시간을 달리하였을 때 icariin, magnoflorine, 엑기스의 추출효율을 분석하였다. Icariin은 $50{\%}$ 에탄올($80^{\circ}C$, 1시간)로 추출하였을 때와 물($100^{\circ}C$, 3시간)로 가열 추출하였을 때 거의 같은 정도로 추출되었으나 magnoflorine은 50% 에탄올로 추출하였을 때 더 많이 추출되었다. 반면에 음양곽의 엑기스량은 50% 에탄올로 추출하였을 때 가장 적었고 물로 추출하였을 때는 추출시간이 길면 길수록 더 많았다. 음양곽을 직접 차(茶)로 음용할 때의 추출 조건에서는 icariin 및 magnoflorine의 추출 효율이 약 25%이므로 음양곽 엑기스로 가공한 차제제가 더 바람직하다고 생각된다. 음양곽 중에 icariin및 magnoflorine의 함량은 각각 0.94, 0.16%임을 처음으로 분석하였다.

• 생약학회지
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• 제17권1호
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• pp.49-54
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• 1986

To explore the biological activities of Thalictrum alkaloids, a study was initiated to investigate the alkaloidal constituents from T. fauriei, a species indigenous to Taiwan. Extensive fractionation of the ethanolic extract provided three known aporphines, (+)-oconovine (Ia), (+)-isocorydine (Ib), and (+)-corydine (Ic) from nonphenolic tertiary base fraction. From quaternary base fraction two new protoberberinium salts, thalifaurine (IIa), dehydrodiscretine (IIb), in addition to magnoflorine (III), were isolated. The structure of both new compounds were confirmed by total syntheses. Fractionation of phenolic bases yielded a dihydromorphinandienone, (-)-ocobotrine (IV), as well as a novel aporphine-pavine dimer, tentatively named EP-10. The structure of EP-10 was established by means of 2D NMR studies. Preliminary study indicated a weak activity of lyzing Hela cells, in vitro, for EP-10.

• 생약학회지
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• 제2권1호
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• pp.19-21
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• 1971

Examination of the alkaloids in the stem of Berberis amurensis $R_{UPRECHT}$ var. latifolia $N_{AKAI}$ was carried out. As tertiary bases, the biscoclaurin type base berbamine and oxyberberine as the berberine type were isolated, and jatrorrhizine, berberine, shobakunine as the berberine type and the aphorphine type magnoflorine were obtained as the quarternary bases.

• 생약학회지
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• 제2호2호
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• pp.89-93
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• 1971

This study is about the five species of the genus Thalictrum growing abundantly in South Korea. Dried roots of plants; T. aquilegifolium var. japonica, T. simplex var. affine, T. actaefolium, T. tuberiferum were compared by morphological observation and screening test on the ingredient extracted by methanol, ether etc.. The experiments by thin-layer chromatography was at tempted to find out the similarity and the difference among them. In conclusion, the author found that: 1) A special component similar to coumarin was found under UV-ray from T. actaefolium. 2) By thin-layer chromatography, they show the corresponding spot supposed to be that of berberine and magnoflorine according to the $T_{ABLE}\;IV$. Therefore it is worthwhile considering that Thalictrum species would be a substitution for Epimedium species used as a tonic. 3) Thalictrum species has resemblance of ingredient and chemotaxonomy gives more help to classify them than the morphology.

• 생약학회지
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• 제43권2호
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• pp.127-136
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• 2012

Eight flavonoids were isolated from the BuOH fraction of the 70% EtOH extract of Zizyphus jujuba var. spinosa seeds along with three known compounds, daucosterol (1), butyl ${\beta}$-D-fructofuranoside (2), and magnoflorine (4). On the basis of spectroscopic methods and comparison with literature values their structures were elucidated as 6"'-feruloylspinosin (3), nicotiflorin (5), 6"'-p-coumaroylspinosin (6), isospinosin (7), 6"'-vanilloylspinosin (8), spinosin (9), hovetrichoside C (10), and camelliaside B (11). Compounds 1, 2, 10, and 11 were isolated from this plant for the first time.

• 약학회지
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• 제45권1호
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• pp.34-38
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• 2001

The Phellodendri Cortex of Phellodendron amurense (Rutaceae) is known to contain a number of isoquinoline alkaloid, and berberine, palmatine, jateorrhizine, phellodendrine and magnoflorine are the major constituents of protoberberine alkaloids. For the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation, the new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. Samples were extracted with 0.1 mM hydrochloric acid, potassium biphthalate reagent, thiocyanatocobaltate reagent and 1.2-dichloroethane for 60 min. The absorbance of protoberberine alkaloid complexes in 1.2-dichloroethane solution was measured at 625 nm. Calibration curve for berberine was linear over the concentration range of 0.05~0.30 mg/ml 1.2-dichloroethane. The method proved to be rapid, simple and reliable for the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation.

• Archives of Pharmacal Research
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• 제29권8호
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• pp.627-632
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• 2006

Chromatographic separation of the MeOH extract from the stems and rhizomes of Sinomemium acutum led to the isolation of nine alkaloids and a lignan. Their structures were determined to be dauriporphine (1), bianfugecine (2), dauriporphinoline (3), menisporphine (4), (-)-syringaresinol (5), N-feruloyltyramine (6), acutumine (7), dauricumine (8), sinomenine (9), and magnoflorine (10) by spectroscopic means. These compounds were examined for their P-gp mediated MDR reversal activity in human cancer cells. Compound 1 showed the most potent P-gp MDR inhibition activity with an $ED_{50}$ value $0.03\;{\mu}g/mL$ and $0.00010\;{\mu}g/mL$ in the MESSA/DX5 and HCT15 cells, respectively.

• 생약학회지
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• 제28권4호
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• pp.257-263
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• 1997

Berberis amurensis Ruprecht(Berberidaceae) is a medicinal plant indigenous to the middle and northern part of Korean peninsula. The woody parts of this plant have been used for the ocular, peptic and intestinal disorders. The stems of this plants were extracted with MeOH and the MeOH extract was partitioned between organic phases and water layer, successively to fractionated quarternary alkaloids. The acetone-soluble part of guarternary alkaloidal fraction had antibacterial activities and it contained four protoberberine alkaloids such as palmatine(I), Berberine(II), Jatrorrhizine(III) and coptisine(IV), and one aporphine alkaloid, magnoflorine(V). Although the isolations of the compounds I, II, IIII, IV and V from different sources were reported, this is the first report that Berberis amurensis contained the compounds. When the contents of compound I(palmatine) and II(berberine) were quantified and compared with those of other plant parts, cortex contained higher palmatine and berberine than any other part of the plant.