• Title/Summary/Keyword: low dose gamma irradiation

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Pretreatment of Low Dose Radiation Reduces Radiation-Induced Apoptosis in Mouse Lymphoma (EL4) cells

  • Kim, Jeong-Hee;Hyun, Soo-Jin;Yoon, Moon-Young;Jioon, Young-Hoon;Cho, Chul-Koo;Yoo, Seong-Yul
    • Archives of Pharmacal Research
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    • v.20 no.3
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    • pp.212-217
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    • 1997
  • Induction of an adaptive response to ionizing radiation in mouse lymphoma (EL4) cells was studied by using cell survival fraction and apoptotic nucleosomal DNA fragmentation as biological end points. Cells in early log phase were pre-exposed to low dose of ${\gamma}$-rays (0.01 Gy) 4 or 20 hrs prior to high dose ${\gamma}$-ray (4, 8 and 12 Gy for cell survival fraction analysis; 8 Gy for DNA fragmentation analysis) irradiation. Then cell survival fractions and the extent of DNA fragmentation were measured. Significant adaptive response, increase in cell survival fraction and decrease in the extent of DNA fragmentation were induced when low and high dose .gamma.-ray irradiation time interval was 4 hr. Addition of protein or RNA synthesis inhibitor, cycloheximide or 5,6-dichloro-1-.betha.-d-ribofuranosylbenzimidazole (DRFB), respectively during adaptation period, the period from low dose ${\gamma}$-ray irradiation to high dose ${\gamma}$-ray irradiation, was able to inhibit the induction of adaptive response, which is the reduction of the extent DNA fragmentation in irradiated EL4 cells. These data suggest that the induction of adaptive response to ionizing radiation in EL4 cells required both protein and RNA synthesis.

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Study on the Crosslinking Characteristics of LDPE and LLDPE by $\gamma$-Ray Irradiation ($\gamma$-선 조사에 의한 LDPE, LLDPE의 가교특성에 관한 연구)

  • 김정일;박성현;강필현;노영창
    • Polymer(Korea)
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    • v.25 no.5
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    • pp.657-664
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    • 2001
  • In this study. the effects of ${\gamma}$-irradiation on the crosslinking of low density poly ethylene (LLDPE) and linear low density polyethylene (LLDPE) containing crosslinking agents were investigated to find the degree of crosslinking in the polymer. The LDPE and LLDPE specimens were prepared by blending crosslinking agents with each polymers, and by hot-press-molding into a sheet at 13$0^{\circ}C$. The ${\gamma}$-irradiation was conducted at 50 to 150 kGy in nitrogen. The crosslinking percentage in these specimens was measured in relation to the irradiation dose and the type of crosslinking agents. The mechanical properties, thermal properties and crystallinity of specimens were examined as a function of irradiation dose as well. It was found that the degree of crosslinking of the irradiated specimens was increased with increasing irradiation dose and by the addition of crosslinking agents. The mechanical properties and thermal properties of specimens were improved in proportion to an increase in the degree of crosslinking. The crystallinity of original resin was decreased with increasing crosslinking density.

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Effects of Gamma Irradiation on Taste Compounds in Processing of Low Salted and Fermented Squid (감마선 이용 저염 오징어젓갈 제조시 정미성분의 변화)

  • 변명우;이경행;김재훈;이주운;이은미;김영지
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1051-1057
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    • 1999
  • The effects of gamma irradiation on taste compounds of low salted and fermented squid were investig ated. Salted and fermented squid products were prepared with salt concentrations of 5%, 10%, and 20% and was fermented at 15oC and 25oC after gamma irradiation with a dosage of 2.5~10 kGy. Amino nitrogen (AN), volatile basic nitrogen(VBN), trimethylamine(TMA), and hypoxanthine(Hx) contents were examined during the fermentation periods. Results showed that gamma irradiation had no effect on the initial con tents of AN, VBN, TMA, and Hx compared with non irradiated salted and fermented squid. During the fermentation periods, these contents rapidly increased in accordance with the decrease in NaCl concen tration and irradiation dose, and the increase in fermentation temperature. Specifically, the taste compounds of salted and fermented squid prepared with a NaCl concentration of 10% and an irradiation dose of 10 kGy maintained the appropriate level for the fermentation period at 15oC.

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Effects of low dose gamma irradiation on the germination and physiological activity of old red pepper ( Capsicum annuum L.) seed (묵은 고추종자의 발아와 생리활성에 미치는 저선량 방사선조사 효과)

  • Kim, Jae-Sung;Back, Myung-Hwa;Lee, Hae-Youn;Lee, Young-Keun
    • Journal of Radiation Protection and Research
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    • v.26 no.4
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    • pp.409-415
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    • 2001
  • To observe the stimulating effects of low dose gamma radiation on the germination and physiological activity of germinating seeds of old red pepper (Capsicum annuum L. cv Jokwang and cv. Hongkwang), seeds were irradiated at the dose of $2{\sim}50 Gy$. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage of induction. The germination rate at 7 days after sowing in Jokwang and Hongkwang cultivar was high as 74% and 11% at 4 Gy and 8 Gy irradiation group, respectively. The seedling height of Jokwang cultivar was noticeably high at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group. The protein contents of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at the late stage of induction and that of Hongkwang cultivar at the early stage of induction. Catalase and peroxidase activities of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group.

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Influence of low dose ${\gamma}$ radiation on the physiology of germinative seed of vegetable crops (저선량 감마선이 채소 발아종자의 생리활성에 미치는 영향)

  • Kim, Jae-Sung;Lee, Eun-Kyung;Back, Myung-Hwa;Kim, Dong-Hee;Lee, Young-Bok
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.58-61
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    • 2000
  • This study was conducted to determine the effect of low dose ${\gamma}-ray$ on the germination rate and physiology of germinative seeds of welsh onion ( Allicm fistulosum L. cv. Sukchangwoidae ) and spinach ( Spinacia oleracea L. cv. Chungrok ). The germination rate of irradiation group was much higher than that of the control. Especially it was noticeably higher in 1 or 2 Gy irradiation groups in the sowing spinach seeds on paper towel. On the welsh onion, the germination rate of the 1 Gy irradiation group increased by 17% compared to that of the control. Ion leakage from seeds irradiated with low dose of ${\gamma}-ray$ was decreased compared to that from the control especially at the early stage of incubation when examined by means of electric conductance. This tendency was confirmed in seeds of welsh onion and spinach. Starch hydrolysis was stimulated by ${\gamma}-ray$ irradiation in welsh onion. Furthermore ${\gamma}-ray$ irradiation was beneficial to keeping the vitality of seeds as determined through decarboxylation of glutamic acid.

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Immunostimulating Effects of Angelica Gigas by Radio-Protective (당귀의 방사선 방호에 의한 생체면역 증진 효과)

  • Kim Kyung-Yoon;Jeong Hyun-Woo;Kim Gye-Yeop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.5
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    • pp.1256-1260
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    • 2005
  • The purpose of this study was to investigate the effects of Angelica gigas on jejunal survival, endogenous spleen colony formation and jejunal crypt cells of mice irradiated with Gamma-ray irradiation. The subject of this study includes 42 mice which were divided into each 7 groups. Angelica gigas experiment groups were Angelica gigas + Gamma-ray(10Gy), Angelica gigas + Gamma-ray(3Gy), Angelica gigas. Gamma-ray(1 Gy), Gamma-ray control (10Gy), Gamma-ray control(3Gy), Gamma-ray control(1Gy), Normal groups. In the present study to evaluate the effect of Angelica gigas on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice Gamma-ray with each dose of Gamma-ray irradiation. The results of this study were as follows: In low-dose(1Gy) Gamma-ray radiation were treatment of Angelica gigas showed significantly increased(p<0.05) on the cell death apoptosis in crypt, intestine crypts survival of intestine after gamma-ray irradiation. High-dose(10Gy) Gamma-ray, treatment of Angelica gigas showed significantly increased(p<0.05) on the leukocyte. The above results suggest that Angelica gigas were immunostimulating effectively reduced Gamma-ray irradiation.

Effect of Low Dose of Gamma Radiation on the Growth of Groundnut (Arachis hypogaea L.) (저선량 감마선이 땅콩 생장에 미치는 효과)

  • 김재성;이은경;백명화;박홍숙;김광호
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.257-261
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    • 1999
  • Effect of low dose gamma radiation on the growth of groundnut (Arachis hypognea L.) were investigated with respect to germination rate, seedling development and yield. Seeds of “Palpal” cultivar were irradiated with 0.5~20 Gy of ${\gamma}$ radiation in order to determine the hermetic effect of low dose radiation. The germination rate of ${\gamma}$-ray irradiation group was lower than that of the control but the seedling height of groundnut grown from seeds irradiated with low dose ${\gamma}$-ray was slightly higher than that of the control. The number of pod and kernels, and the seed yield increased by 27%, 17% and 19 %, respectively, in the 12.0 Gy irradiation group compared to that in the control group. The 100 seed weight was 87.2 g in the 4.0 Gy irradiation group, which was 11% heavier than 78.3 g in the control group. Low dose radiation showed an enhancement effects on the growth and yield components of groundnut.

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Reducing Effect of Microorganism on Meat and Fish Products by Repeated γ-Irradiation at Low Dose (저선량 감마선 반복조사의 육류와 생선 중 미생물 저감효과)

  • Je, Gil-Soo;Chung, Duck-Hwa;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.30 no.1
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    • pp.92-97
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    • 2015
  • The aim of this study is to examine the removal efficiency of pathogen (Escherichia coli O157:H7 and Salmonella typhimurium) on meat and fish products (packing condition: vacuum or not and storage temperature: $4^{\circ}C$ or $-20^{\circ}C$) repeatedly exposed at low-dose gamma irradiation. In case of meat products (beef and chicken), E. coli O157:H7 was not observed at the level of 2 kGy single gamma irradiation and 0.5 kGy repeated gamma irradiation and S. Typhimurium was not observed at the level of 2 kGy single gamma irradiation and 1 kGy repeated gamma irradiation. In case of fish products, E. coli O157:H7 and S. Typhimurium were not detected at the level of 0.5 kGy single and repeated gamma irradiation. These results showed that microorganisms on fish products were more efficiently removed than those of meat products with low-dose gamma irradiation. Generally, each packing condition made no difference. However, the products (fish and meat) stored at $-20^{\circ}C$ needed more higher dose gamma irradiation than products at $4^{\circ}C$.

Effect of Gamma Irradiation on Botrytis cinerea Causing Gray Mold and Cut Chrysanthemum Flowers

  • Chu, Eun-Hee;Shin, Eun-Jung;Park, Hae-Jun;Jeong, Rae-Dong
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.193-200
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    • 2015
  • Gray mold caused by Botrytis cinerea is one of the most important postharvest fungal pathogens of cut flowers. Here, gamma irradiation, an alternative for phytosanitary purposes, and sodium dichloroisocyanurate (NaDCC) were used to control B. cinerea in a cut chrysanthemum (Chrysanthemum morifolium Ramat.) cultivar, 'Baekma', one of the cultivars susceptible to B. cinerea. Spore germination and mycelium growth of B. cinerea were inhibited by gamma irradiation in an inversely dose-dependent manner. A dose of 4 kGy completely inhibited the mycelium growth of B. cinerea. A significant change in flower quality (physical properties) on chrysanthemum was shown from gamma irradiation at over 0.2 kGy (p<0.05). Therefore, in this study, the integration of gamma ray (below 0.2 kGy) and NaDCC, an eco-friendly form of chlorine, was investigated to control the disease with low dose of gamma irradiation dose. Interestingly, the gamma irradiated flowers showed more disease severity than the non-irradiated flowers. The combined treatment of gamma irradiation and NaDCC does not affect the severity of the fungal disease, whereas only 70 ppm of NaDCC treatment showed a significantly reduced severity. These results suggest that only chlorination treatment can be applied to control B. cinerea in cut chrysanthemum flowers.

The Dielectric Properties due to the Temperature and Applied Voltage of Oriented Polypropylene Film Irradiated by $^{60}/Co-${\gamma}$$ ($^{60}/Co-${\gamma}$$선으로 조사된 이축연신 폴리프로필렌 필림의 온도 및 전압에 따른 유전특성)

  • 홍진웅;이수원;김왕곤
    • Journal of the Korean Society of Safety
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    • v.9 no.1
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    • pp.48-55
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    • 1994
  • In this paper, It is studied that the variation of the dielectric absorption of the specimen according to the change with $^{60}$ Co-${\gamma}$ ray irradiation dose of the influence of temperature and applied voltage. In order to investigate the effect of irradiated oriented polypropylene film, we have observed dielectric properties within the temporature range of 30~130 ($^{\circ}C$) and voltage range of 100~250 (V). As for the dependency of temperature by tan $\delta$, the $\alpha$ peak which appears at high temperature increases accordingly to the increment of irradiation dose which is contributed by the crystal region and moves towards the high temperature. The $\beta$ peak which appears the orientation by dipoles and molecular motions in the amorphous region at low temperature. For the dependency of voltage by tan $\delta$ at low temperature, the peak of the tan $\delta$ shifts accordingly to the increment of irradiation dose towards the high temperature region.

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