• Title/Summary/Keyword: livers

Search Result 486, Processing Time 0.022 seconds

A Study on the Effect of Chitin, Chitosan and Dithiocarbamate Chitosan on the Nickel Toxicity in Rat liver

  • Yoo, Il-Sou;Choi, Kyung-Soon;Ryu, Mun-Hee
    • Journal of Environmental Health Sciences
    • /
    • v.34 no.4
    • /
    • pp.285-291
    • /
    • 2008
  • This study was performed to investigate the effects of Chitosan on the nickel poisoning in rats. In the study, 150 male Sprague-Dawley were used. The experimental groups were divided into four: A (30 mg/L nickel), B (30 mg/L nickel+0.2% Chitin, Chitosan and Dithiocarbamate Chitosan), C (30 mg/L nickel+0.4% Chitin, Chitosan and Dithiocarbamate Chitosan), D (30 mg/L nickel+0.8%Chitin, Chitosan and Dithiocarbamate Chitosan). The results were as flows; 1. The nickel concentration in the livers of the control group (A) was $0.153{\sim}0.186\;mg/kg$ but the nickel concentration in the livers of the experimental decreased during the experimental period (P<0.05). 2. Metallothionenin levels in rat liver were $2.77{\sim}3.25\;ug/g$ wet,wt in control group (A), but were $2.89{\sim}3.51\;ug/g$ wet,wt (B), $2.97{\sim}3.62\;ug/g$ wet,wt (C), $2.68{\sim}3.68\;ug/g$ wet,wt (D). Respectively in the experimental groups. The experimental groups were inclined to increase compare to the control group (P<0.05). In conclusion, this study revealed a preventive effect of Chitin, Chitosan and Dithiocarbamate Chitosan against nickel toxicity.

Differential Expression Patterns of Gangliosides in the Liver and Heart of NIH-miniature Pigs (NIH-미니돼지의 간과 심장에서 갱글리오시드의 서로 다른 발현 패턴)

  • Ryu, Jae-Sung;Chang, Kyu-Tae;Kim, Ji-Su;Kwak, Dong-Hoon;Lee, Young-Choon;Oh, Keon-Bong;Choo, Young-Kug
    • Journal of Life Science
    • /
    • v.20 no.4
    • /
    • pp.467-473
    • /
    • 2010
  • Gangliosides are a major component of the plasma membrane of mammalian cells, which are directly involved in a variety of immunological events, including cell-to cell or cell-to-protein interactions. In this study, we investigated whether gangliosides, sialic acid-containing glycosphingolipids, are related to rejection during the xenotransplantation of NIH-miniature pig livers and hearts to humans. Both high performance thin-layer chromatography and immunohistochemistry analyses revealed that the expression of gangliosides in the liver tissue of NIH-miniature pigs was higher than that in the heart. Gangliosides GD3, GD1a, GD1b, GT1b and GQ1b were observed in both the liver and heart, whereas GQ1b was detected only in the liver, indicating that the ganglioside expression profiles are tissue specific. Moreover, other ganglio-series gangliosides, including GM3, were not detected in the livers and hearts of NIH-miniature pigs. Taken together, these results suggest that gangliosides may play important roles in immune responses in clinical xenotransplants of pig livers and hearts.

Effects of Lespedeza Caneata ethanol extract on the Liver, Kidneys of Lead administered Mice (야관문(Lespedeza Caneata) Ethanol 추출물이 납 투여한 생쥐의 간장과 콩팥에 미치는 영향)

  • Chung, Kyoung-A;Cheong, Min-Ju
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.17 no.6
    • /
    • pp.207-214
    • /
    • 2016
  • This study determined the effect of Lespedeza Caneata extract on the livers and kidneys of lead-administered mice. The experimental groups were divided into a normal group, Pb 4W group, Pb-LC 4W group, Pb 8W group, and Pb-LC 8W group. The normal group was supplied single distilled water, and the pb group was provided distilled water in which lead acetate was dissolved at 1,000 ppm. The Pb-LC group was provided with lead as drinking water, and the Lespedeza Caneata was orally medicated at a concentration of 500 mg/kg daily. AST, ALT, and BUN enzyme activities and histological experiments on the livers and kidneys resulted in the following conclusions. AST, ALT, and BUN activities increased in the experimental group compared to the normal group and decreased more in the Pb-LC group than the pb group during the same period. The histological results reveal that portions of the livers and kidneys were deformed in the Pb 4W group, and most of the Pb 8W group experienced necrosis and deformation. pb-LC4W and Pb-LC 8W groups experienced less deformation than the Pb 4W and Pb 8W groups. During the same period, the Pb-LC group experienced less histological changes than the Pb group. These results suggest that Lespedeza Caneata extract may have some protective effect on hepatic tissue and renal tissue damage with lead-administered in mice.

Biophoton Emission as a Biomarker of Mouse Fatty Liver induced by Carbon Tetrachloride (사염화탄소를 이용한 마우스 지방간에서의 새로운 생체발광 측정법)

  • 김정대;임재관;최준호;이병천;유혜란;박대훈;천병수;소광섭
    • KSBB Journal
    • /
    • v.19 no.1
    • /
    • pp.12-16
    • /
    • 2004
  • Recently the measurement of biophoton emission has attracted increasing attention in the study on physiological state of biological systems. We report the measurements of biophoton emission from the mouse fatty liver induced by carbon tetrachloride, CCl$_4$. The hepatotoxin, CCl$_4$ in olive oil, was injected intraperitoneally into two groups of ICR mice which were made of 6 mice in each group. The control groups corresponding to the treated groups were prepared with the injections of olive oil only. After the injections, livers of two groups were extracted and measured biophoton emission in 24 hours and 72 hours later, respectively. We also extracted the plasma in the blood and measured the transaminase activity. Results show that biophoton emission from the livers in 24-hour treated group is 69.3${\pm}$21.2 counts/min/$\textrm{cm}^2$, which is two times more larger than that in 24-hour control group, 29.5${\pm}$5.9 counts/min/$\textrm{cm}^2$ Biophoton emission from the livers in 72-hour treated group is 37.0${\pm}$14.8 counts/min/$\textrm{cm}^2$. These biophoton results correlate with those of the biochemical assays. We conclude that biophoton emission can be used as a biomarker of mouse fatty liver induced by CCl$_4$.

Effects of Ammoniation or Supplementation of BHT, CTC, and Probiotics to Moldy Corn on the Performance of Broiler Chickens (곰팡이 쓴 옥수수를 사용할때 Ammonia 처리와 BHT, CTC, Probiotics의 첨가가 육계에 미치는 영향)

  • 남궁환;백인기
    • Korean Journal of Poultry Science
    • /
    • v.13 no.2
    • /
    • pp.221-226
    • /
    • 1986
  • In order to study the effect of feeding moldy corn in the feed and to compare the efficacy of several antidotical treatments, a feeding trial was conducted using broiler chickens. Ground yet low corn was adjusted to contain 20% moisture and then incubated at 35$^{\circ}C$ for 20 days. The number of fungi increased from 11${\times}$10$^4$/g to 42 10$\^$7/g on incubation but aflatoxins (B$_1$, B$_2$, G$_1$, G$_2$) were not detected by TLC test. Moldy corn was included in the experimental diets at the level of 7% with various treatments. Thirty six hatched male broiler chickens were divided into 6 groups and each group was placed for 4 weeks on one of the following treatments: Fresh corn, Moldy corn, Ammoniated (1.5%) moldy corn, Moldy corn+BHT (0.1%), Moldy corn CTC (100ppm) and Moldy corn+Probiotics. Weight gains of the birds fed Ammoniated moldy corn diet, Moldy corn+Probiotics dist and Moldy corn+CTC diet were greater by 6.1%. 7.6% and 3.9% respectively than those fed Moldy corn diet. The feed efficiencies of Moldy corn+Probiotics diet and Moldy corn+CTC diet were better than those of Moldy corn diet. Dry matter contents of the livers of the birds fed Ammoniated moldy corn diet, Moldy corn+BHT diet and Moldy corn diet were lower than those of other treatments. Proteion contents of the livers of the birds fed Moldy corn diet, Moldy corn +CTC diet and Moldy corn+BHT diet were lower than those of other treatments. Moldy corn tended to increase fat content of the livers.

  • PDF

Effect of Clonorchis sinensis infection on hepatic oval cell proliferation in rats (랫트에서 Clonorchis sinensis 감염이 간 oval cell 의 증식에 미치는 영향)

  • Lee, Jae-Hyun;Yoon, Byung-Il;Park, Min-Kyung;Kim, Shin-A;Lee, Joon-Sang;Joo, Kyoung-Hwan;Kim, Han-Jong
    • Korean Journal of Veterinary Pathology
    • /
    • v.2 no.2
    • /
    • pp.95-106
    • /
    • 1998
  • The prevalence of primary liver carcinoma (PLC) is relatively high in Clonorchis sinensis (CS) endemic areas in Korea. PLC is a malignant tumor which can be subclassified into hepatocellular carcinoma and cholangiocarcinoma(CC). CC has been associated with clonorchiasis, but it is unclear whether clonorchiasis is associated with hepatocarcinogenesis. This experiment was designed to investigate relationships between clonorchiasis and early changes of hepatocarcinogenesis. Sixteen Sprague-Dawley rats weighing 150g were divided into two groups of 8 rats in each. All rats were fed choline-devoid(CD) diet for 4 weeks. Group 1 was given 0.015-0.020% diethylnitrosamine(DEN) as drinking water for 1 week. After one week, the rats were treated orally with 1% N-acetylaminofluorene(AAF) (5 times per week for 2 weeks). Group 2 was treated equally to group 1 except for CS infection during AAF treatment. Two rats in each group were sacrificed at 4th, 5th, 6th and 7th week of the experiment. Livers were stained with OV -6, proliferating cell nuclear antigen(PCNA) and GST-p. Results were as follows: Group 2 livers showed more oval cell proliferation in parenchyma and portal areas at the 4th, 5th, 6th and 7th weeks than did livers of group 1 (p<0.01). PCNA was mostly localized in oval cell populations, rather than hepatocytes and biliary cells. The ratio of oval cells to hepatocytes was much higher in group 2 than in group l(p<0.01 The ratio of hepatocytes to biliary cells is higher in group 2 than in group 1 (p<0.05), More group 2 acidophilic foci reacted to GST-p monoclonal antibody than in the noninfected group. It appeared that CS infection promoted potentially precancerous acidophilic foci and oval cell proliferation.

  • PDF

Effects of Consecutive Lead-Administration on Testis Weights, Number and Motility of Sperm, Organ Weights and Histological Changes in Rats (연속적인 Pb투여가 Rat의 정소중량, 정자수와 활력, 장기 중량 및 조직변화에 미치는 영향)

  • 김상근;이명헌
    • Korean Journal of Animal Reproduction
    • /
    • v.25 no.2
    • /
    • pp.147-153
    • /
    • 2001
  • This study was performed to elucidate the effects of lead poisoning on the reproductive organ of rats. After consecutive oral administrations of lead acetate, the weights of testis, the numbers and motilities of sperms and histological changes of organs were compared between control and experimental groups. 1. Testis weights of 1,000, 2,000 or 4,000 ppm/kg of lead acetate-administrated rats decreased compared with control group in dose-dependent manner. 2. The sperm numbers of 1,000, 2,000 or 4,000 ppm/kg of lead acetate-administrated rats were lowered significantly in dose dependent manners than those of control groups. 3. The sperm motilities of 1,000, 2,000 or 4,000 ppm/kg of lead acetate-administrated rats decreased in dose-dependent manners compared with those of control groups. 4. The weights of livers and kidneys of 1,000, 2,000 or 4,000 ppm/kg of lead acetate-administrated rats decreased or increased. The weights of livers increased and the kidney weights decreased and changes were dose-independent manner. 5. Necrosis of hepatocytes around the central veins, infiltrations of neutrophils, accumulations of bile and infiltrations of fine granules-harboring macrophages in psychymal and interstitial tissues were found out in the livers of copper sulfate-administrated rats. The Bowman's capsule, tubular epithelium and includes in nucleus of kidneys were filled with hyaline materials and hematophilic centers appeared in several lymph nodes.

  • PDF

Apoptosis of livers induced by D-galactosamine and lipopolysaccharide in mice (Mice에서 D-galactosamine과 lipolysaccharide의 유도에 의한 간장의 apoptosis)

  • Kwak, Soo-dong;Kim, Chong-sup;Kang, Chung-boo;Koh, Phil-ok;Seo, Deuk-Iok;Yang, Je-hoon
    • Korean Journal of Veterinary Research
    • /
    • v.40 no.2
    • /
    • pp.213-220
    • /
    • 2000
  • Experimental induction of apoptosis by bacterial lipopolysacchardie is useful for understanding the role of apoptosis cell death in clinical endotoxin shock or septic shock. Thirty three mice were injected intraperitoneally with D-galactosamine (20mg) and lipopolysac-charide ($5{\mu}g$) per mouse. Five to eight mice per each experimental group were sacrificed at 6, 12, 24, 48 and 72 hrs post administration. The cells with apoptotic bodies in H-E stained sections were investigated histologically. Development of the apoptotic bodies in livers was observed in 11 of 33 mice (33.3%). These cells were diffusely or collectively appeared only in liver but not observed in kidney, thymus and spleen. Mean percentage of the cells with apoptotic bodies in the livers were 0.32, 4.34 and 5.50% respectively at 6, 12, and 24 hrs post administration. But percentage of these apoptotic cells were fairly less in 48 and 72 hrs post administration. The percentages of cells with 3 to 9 apoptotic bodies per cell were 70~90% of all apoptotic cells. The cells with more apoptotic bodies than limit number at 12 to 72 hrs post administration were belived to be necrosed. The percentage of positive cells by TUTNEL methods were 0.00~0.08, 0.00~052, 1.63~4.18, 12.41~20.21 respectively at control, 6, 12 and 24 and less than 0.01% at 48 and 72 hrs. The above results suggest that development of liver cell apoptosis by lipopolysaccharide ($5{\mu}g$) and D-glatosamine (20mg) was less at 6 hrs and markedly increased at 12 to 24 hrs and then was fairly less at 48 and 72 hrs post administration.

  • PDF

Fermented ginseng, GBCK25, ameliorates steatosis and inflammation in nonalcoholic steatohepatitis model

  • Choi, Naeun;Kim, Jong Won;Jeong, Hyeneui;Shin, Dong Gue;Seo, Jeong Hun;Kim, Jong Hoon;Lim, Chae Woong;Han, Kang Min;Kim, Bumseok
    • Journal of Ginseng Research
    • /
    • v.43 no.2
    • /
    • pp.196-208
    • /
    • 2019
  • Background: Nonalcoholic steatohepatitis (NASH) is one of the chronic inflammatory liver diseases and a leading cause of advanced liver fibrosis, cirrhosis, and hepatocellular carcinoma. The main purpose of this study was to clarify the effects of GBCK25 fermented by Saccharomyces servazzii GB-07 and pectinase, on NASH severity in mice. Methods: Six-wk-old male mice were fed either a normal diet (ND) or a Western diet (WD) for 12 wks to induce NASH. Each group was orally administered with vehicle or GBCK25 once daily at a dose of 10 mg/kg, 20 mg/kg, 100 mg/kg, 200 mg/kg, or 400 mg/kg during that time. The effects of GBCK25 on cellular damage and inflammation were determined by in vitro experiments. Results: Histopathologic analysis and hepatic/serum biochemical levels revealed that WD-fed mice showed severe steatosis and liver injury compared to ND-fed mice. Such lesions were significantly decreased in the livers of WD-fed mice with GBCK25 administration. Consistently, mRNA expression levels of NASH-related inflammatory-, fibrogenic-, and lipid metabolism-related genes were decreased in the livers of WD-fed mice administered with GBCK25 compared to WD-fed mice. Western blot analysis revealed decreased protein levels of cytochrome P450 2E1 (CYP2E1) with concomitantly reduced activation of c-Jun N-terminal kinase (JNK) in the livers of WD-fed mice administered with GBCK25. Also, decreased cellular damage and inflammation were observed in alpha mouse liver 12 (AML12) cells and RAW264.7 cells, respectively. Conclusion: Administration of GBCK25 ameliorates NASH severity through the modulation of CYP2E1 and its associated JNK-mediated cellular damage. GBCK25 could be a potentially effective prophylactic strategy to prevent metabolic diseases including NASH.

Systemic TM4SF5 overexpression in ApcMin/+ mice promotes hepatic portal hypertension associated with fibrosis

  • Joohyeong, Lee;Eunmi, Kim;Min-Kyung, Kang;Jihye, Ryu;Ji Eon, Kim;Eun-Ae, Shin;Yangie, Pinanga;Kyung-hee, Pyo;Haesong, Lee;Eun Hae, Lee;Heejin, Cho;Jayeon, Cheon;Wonsik, Kim;Eek-Hoon, Jho;Semi, Kim;Jung Weon, Lee
    • BMB Reports
    • /
    • v.55 no.12
    • /
    • pp.609-614
    • /
    • 2022
  • Mutation of the gene for adenomatous polyposis coli (APC), as seen in ApcMin/+ mice, leads to intestinal adenomas and carcinomas via stabilization of β-catenin. Transmembrane 4 L six family member 5 (TM4SF5) is involved in the development of non-alcoholic fatty liver disease, fibrosis, and cancer. However, the functional linkage between TM4SF5 and APC or β-catenin has not been investigated for pathological outcomes. After interbreeding ApcMin/+ with TM4SF5-overexpressing transgenic (TgTM4SF5) mice, we explored pathological outcomes in the intestines and livers of the offspring. The intestines of 26-week-old dual-transgenic mice (ApcMin/+:TgTM4SF5) had intramucosal adenocarcinomas beyond the single-crypt adenomas in ApcMin/+ mice. Additional TM4SF5 overexpression increased the stabilization of β-catenin via reduced glycogen synthase kinase 3β (GSK3β) phosphorylation on Ser9. Additionally, the livers of the dualtransgenic mice showed distinct sinusoidal dilatation and features of hepatic portal hypertension associated with fibrosis, more than did the relatively normal livers in ApcMin/+ mice. Interestingly, TM4SF5 overexpression in the liver was positively linked to increased GSK3β phosphorylation (opposite to that seen in the colon), β-catenin level, and extracellular matrix (ECM) protein expression, indicating fibrotic phenotypes. Consistent with these results, 78-week-old TgTM4SF5 mice similarly had sinusoidal dilatation, immune cell infiltration, and fibrosis. Altogether, systemic overexpression of TM4SF5 aggravates pathological abnormalities in both the colon and the liver.