Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on the Effect of Chitin, Chitosan and Dithiocarbamate Chitosan on the Nickel Toxicity in Rat liver

  • Yoo, Il-Sou (Department of Polymer-Nano Science and Technology, Chonbuk National University) ;
  • Choi, Kyung-Soon (Department of Food and Nutrition Sahm Yook University) ;
  • Ryu, Mun-Hee (Department of Food Science and Biotechnology Chonbuk National University)
  • Published : 2008.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the effects of Chitosan on the nickel poisoning in rats. In the study, 150 male Sprague-Dawley were used. The experimental groups were divided into four: A (30 mg/L nickel), B (30 mg/L nickel+0.2% Chitin, Chitosan and Dithiocarbamate Chitosan), C (30 mg/L nickel+0.4% Chitin, Chitosan and Dithiocarbamate Chitosan), D (30 mg/L nickel+0.8%Chitin, Chitosan and Dithiocarbamate Chitosan). The results were as flows; 1. The nickel concentration in the livers of the control group (A) was $0.153{\sim}0.186\;mg/kg$ but the nickel concentration in the livers of the experimental decreased during the experimental period (P<0.05). 2. Metallothionenin levels in rat liver were $2.77{\sim}3.25\;ug/g$ wet,wt in control group (A), but were $2.89{\sim}3.51\;ug/g$ wet,wt (B), $2.97{\sim}3.62\;ug/g$ wet,wt (C), $2.68{\sim}3.68\;ug/g$ wet,wt (D). Respectively in the experimental groups. The experimental groups were inclined to increase compare to the control group (P<0.05). In conclusion, this study revealed a preventive effect of Chitin, Chitosan and Dithiocarbamate Chitosan against nickel toxicity.

Keywords

References

  1. Park, H. S., and Park, K. S. : Nickel Toxicity and Carcinogenicity J. Environ. Toxicol. 19(2), 119-134, 2004
  2. Cha, B. S., Wang, S. J., Chang, S. J. and Lee, J. W. : Comparative Hepatotoxicity Assessment of Cadmium and Nickel with Isolated Perfused Rat Liver. Korean J. Perv. Med. 33(1), 117-124, 2000
  3. Lee, K. N., Il-soo You, I. S. and Lee, J. S. : A study on Antitoxic Effect of Natural products Against Nickel Toxicity. Kor. J. Env.. Halth. Soc., 20(3), 85-95, 1994
  4. Hall L. D. and Yalpani M. : Enhancement of the metal chelating properties of chitin and chitosan. Carbohydrate Research, 83, C5-C7, 1980 https://doi.org/10.1016/S0008-6215(00)85386-0
  5. Eaton, D. L. and Toal, B. F. : Evaluation of Cd/ henolobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicol Appl Pharmacol., 66, 134, 1982 https://doi.org/10.1016/0041-008X(82)90068-0
  6. Hall, L. D. and yalpani, M. : Enhancement of the metal chelating properties of chitin and chitosan. Carbohydrate Research, 83, C5-C7, 1980 https://doi.org/10.1016/S0008-6215(00)85386-0
  7. Kage, JHR. and Vallee, BL. : A cadmium and zinccontaining protein from equine renal cortex. J. Biol. Chem. 236, 2435-2442, 1961
  8. Maruca, R. suder, BJ. and Wightman, J. : Interaction of heavy metals with chitin and chitosan. Chromium J. of Applied Polymer Science, 27, 4827-4837, 1982 https://doi.org/10.1002/app.1982.070271227
  9. Pulido, P. Kagi, J. and Vallee BL. : Isolation and some propertie of human metallothionein Bioch7. Cherian MG, Nordberg M: Cellular adaptation in metal toxicolgy and metallothionein. Toxicology, 25, 1-15, 1983 https://doi.org/10.1016/0300-483X(82)90079-8
  10. Landes, D. R. and Bough, W. A. : Effects of chitosan acoggulating agent for food processing wastes in the diet of rats on growth and liver and blood consumption. Bull. Environ. Contam. Toxicol., 15, 55-57, 1976 https://doi.org/10.1007/BF01686196
  11. Eaton, D. L. and Toal, B. F. : Evaluation of the Cd/ hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicol. Appl. Pharmacol., 5, 1768-1777, 1966
  12. Kim, S. K. and Lee, E. H. : Biocompatibility and Medical Applications of Chitin and Chitosan. Korean J. Chitin and Chitosan, 2(2), 39-74, 1997
  13. Cherian, M.G. and Nordberg, M. : Cellular adaptation in metal toxicolgy and metallothionein. Toxicology, 25, 1-15, 1983 https://doi.org/10.1016/0300-483X(82)90079-8
  14. Kakela, R. and Kakela, A.: Effects of nickel chloride on reproduction of the rat and possible antagonistic role of selenium. J. Biol. Chem. 236, 2516-2525, 1961
  15. Kostonis, F. N. and Klaassen, C. D. : Toxicity and distribution of cadmium administered to rats at sublethal doses. Toxicol Appl Pharinacol., 41, 667, 1977 https://doi.org/10.1016/S0041-008X(77)80020-3
  16. Kim, Y. T. and You, J. H. : Yun-Jun Jung, Jeoung- Soo Lee and Young-Mi Kim. Development of Polymeric Drugs Vtilizing Dithiocarbamate chitosan. Yakhak hoeji, 39(4), 373-379, 1995
  17. Saplakoglu, U. : DNA single-strand breakage in rat lung, liver and kidney after single and combined treatments of nickel and cadmium, Mutation research. Genetic Toxicology and Environmental Mutagenesis, 394(1), 133-140, 1997 https://doi.org/10.1016/S1383-5718(97)00134-4
  18. Onosaka, S., Tanak, K., Doi, M. and Okahara, K. : A simplified procedure for determination of metallothionein in animal tissues. Eisei Kagaku 24, 128-133, 1978 https://doi.org/10.1248/jhs1956.24.128
  19. Jung, K. S., Kim, D. H. and Jung, K. D. : Effect of Dietary calcium, Casein and Suet on the Accumulation of cadmium in Mice. Korean J. of Preventive Medicine, 21(2), 340-350,1988