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Development of a cell-laden thermosensitive chitosan bioink for 3D bioprinting

  • Ku, Jongbeom;Seonwoo, Hoon;Jang, Kyoung-Je;Park, Sangbae;Chung, Jong Hoon
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.107-107
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    • 2017
  • 3D bioprinting is a technology to produce complex tissue constructs through printing living cells with hydrogel in a layer-by-layer process. To produce more stable 3D cell-laden structures, various materials have been developed such as alginate, fibrin and gelatin. However, most of these hydrogels are chemically bound using crosslinkers which can cause some problems in cytotoxicity and cell viability. On the other hand, thermosensitive hydrogels are physically cross-linked by non-covalent interaction without crosslinker, facilitating stable cytotoxicity and cell viability. The examples of currently reported thermosensitive hydrogels are poly(ethylene glycol)/poly(propylene glycol)/poly(ethylene glycol) (PEG-PPG-PEG) and poly(ethylene glycol)/poly(lactic acid-co-glycolic acid) (PEG/PLGA). Chitosan, which have been widely used in tissue engineering due to its biocompatibility and osteoconductivity, can be used as thermosensitive hydrogels. However, despite the many advantages, chitosan hydrogel has not yet been used as a bioink. The purpose of this study was to develop a bioink by chitosan hydrogel for 3D bioprinting and to evaluate the suitability and potential ability of the developed chitosan hydrogel as a bioink. To prepare the chitosan hydrogel solution, ${\beta}-glycerolphosphate$ solution was added to the chitosan solution at the final pH ranged from 6.9 to 7.1. Gelation time decreased exponentially with increasing temperature. Scanning electron microscopy (SEM) image showed that chitosan hydrogel had irregular porous structure. From the water soluble tetrazolium salt (WST) and live and dead assay data, it was proven that there was no significant cytotoxicity and that cells were well dispersed. The chitosan hydrogel was well printed under temperature-controlled condition, and cells were well laden inside gel. The cytotoxicity of laden cells was evaluated by live and dead assay. In conclusion, chitosan bioink can be a candidate for 3D bioprinting.

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A Study of Ending Credit in Animations-Focused on Credit Cookie (극장판 애니메이션의 엔딩 크레딧 양상연구: 쿠키 영상을 중심으로)

  • Park, Sung-Won;Lee, Hye-won
    • Journal of Information Technology Applications and Management
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    • v.27 no.1
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    • pp.187-198
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    • 2020
  • With the development of technology and the creation of an entertainment environment for leisure, various marketing strategies are being used in the film industry. Among them, the use of the credit cookie of ending credits was very effective in producing the series. The ending credit is the time it takes to show the names of the people who made the movie, which is meaningless to the audience. There is a cost to produce a ending credit but It wasn't made because no revenue was generated. The credit cookie was inserted into this ending credit area, which brought new pleasure to the audience. Most of them were epilogue images showing the story behind the movie, NG images showing the NG situation during film production, and In videos mentioned in the movie but not shown in the movie itself. As various ideas about credit cookie were connected with marketing, a series movie and a spin-off foretelling the derivative works after the screening work were produced and have a new meaning. As a result, the time of ending credits, which had no commercial value, became the methodology of the most powerful promotional strategy. Looking at the difference between live-action film and animation in producing such credit cookie, unlike live-action films that edit the remaining parts after shooting, the NG video of the animation has a lot of time and money to produce. So, it hasn't try very well, and it seems to have been actively produced when moving from 2D animation to 3D animation. This is because 3D animation, which has already been modeled, can create new NG scenes by simply adding animating based on the layout of the created scene. Since it is possible to produce an episode movie at a low cost and time, and to use the scenes of the movie after the production, it will be necessary to strategically produce credit cookie for promotion in animation.

The Prospects of the Married Men and Women in their 50s and 60s about their Future Coresident Family Members, Caregivers, and Residence (50~60대 기혼 남녀의 노년기 가족생활 전망:동거 가족, 돌봄자, 거주지 전망과 관련된 요인 탐색)

  • Chin, Meejung;Sung, Miai;Byun, Joosoo
    • Journal of Families and Better Life
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    • v.32 no.1
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    • pp.27-41
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    • 2014
  • This study attempted to explain how middle-aged married men and women prospected their family life in terms of their future coresident family members, caregivers, and residence, and what factors were associated with these prospects. The prospects reflected their realistic expectation rather than their preference based on their current life situations. Data were drawn from a survey of 800 married men and women in their 50s and 60s in Seoul and Gyunggi-do. Following previous research, we examined how resources (age, sex, health status, spouse's health status, number of children, current living arrangement, and household income), subjective perception on their responsibility for their parents and children, and relational satisfaction with their spouse and with their children were associated with the prospect. The results showed that these factors were associated with the prospect which is with whom they would live, who would care for them, and where they would live in different ways. The resources were more likely to be associated with the prospect on coresident family members and residence. The perceptions on responsibility were more likely to be associated with the prospect on caregivers. The relational satisfaction was more likely to be associated with the prospect on coresident members. These results underscored that the characteristics of caregiving and family life would change in 10-20 years. Family policymakers need to take these changes into consideration as they deal with issues of family policy.

An Analysis on Factors Affecting Korean Elderly People's ADL (우리나라 노인인구의 ADL 결정요인에 관한 연구)

  • Lee, Mee-Ae
    • The Korean Journal of Community Living Science
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    • v.21 no.2
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    • pp.201-210
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    • 2010
  • Over the past few decades, the proportion of elderly people in Korea has been rapidly increasing. In particular, rural areas are experiencing aging of communities more rapidly compared to urban areas. However, public policy for the elderly does not respond to the needs of rural elderly. To distribute health care resources equitably, it is necessary to gather reliable information on the health status of the elderly. The purpose of this study is to explore factors affecting Korean elderly people's ADL functional status. The data sources are from 2004 Elderly Living Condition Survey. The Analysis sample consists of 3,278 cases. Analysis results show that there is a significant residential variability in education, monthly stipend, living arrangement, subjective health status, regular food in-take, and regular exercise. Logistic regression analysis results also show that 'cognitive ability'(exp(B)=6.603), 'subjective health status'(exp(B)=4.576), 'age'(exp(B)=2.610), and 'living arrangement'(exp(B)=.589) are factors affecting ADLs. Namely, when a respondent's cognitive ability is limited, subjective health status is poor, or if their age is over 75, the probability of having a limited ADL has been 6.6 times, 4.6 times, and 2.6 times higher than otherwise. Among these variables, cognitive ability was the best explanation. In contrast, respondents who live with a spouse or adult children have a lower probability of having limited ADL compared with those who live alone. Considering that the most critical criteria in determining eligibility for social welfare services is ADLs, the development of appropriate ADL assessment tools is in an urgent need. Without the accurate assessment on ADLs, particularly on rural as well as the urban elderly, it seems to be hard to achieve effectiveness in the health care policy for the elderly.

A study on the detection of DDoS attack using the IP Spoofing (IP 스푸핑을 통한 DDoS 공격 탐지 방안에 대한 연구)

  • Seo, Jung-Woo;Lee, Sang-Jin
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.25 no.1
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    • pp.147-153
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    • 2015
  • Since the DoS(Denial of Service) attack is still an important vulnerable element in many web service sites, sites including public institution should try their best in constructing defensive systems. Recently, DDoS(Distributed Denial of Service) has been raised by prompting mass network traffic that uses NTP's monlist function or DoS attack has been made related to the DNS infrastructure which is impossible for direct defense. For instance, in June 2013, there has been an outbreak of an infringement accident where Computing and Information Agency was the target. There was a DNS application DoS attack which made the public institution's Information System impossible to run its normal services. Like this, since there is a high possibility in having an extensive damage due to the characteristics of DDoS in attacking unspecific information service and not being limited to a particular information system, efforts have to be made in order to minimize cyber threats. This thesis proposes a method for using TTL (Time To Live) value in IP header to detect DDoS attack with IP spoofing, which occurs when data is transmitted under the agreed regulation between the international and domestic information system.

A Study on the Development of Realtime Online Maketing System Using Web Log Analytics (웹 로그분석을 이용한 실시간 온라인 마케팅 시스템 설계 및 개발에 관한 연구)

  • Oh, Jae-Hoon;Kim, Jae-Hoon;Kim, Jong-Woo
    • The Journal of Society for e-Business Studies
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    • v.16 no.3
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    • pp.249-261
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    • 2011
  • The rapid growth of e-business market makes new online companies to start and existing offline companies to join in this area. As the number of players of this market grows rapidly, the competition among them is very intense. Many companies invest huge resources to online marketing including search advertisement, email advertisement and banner advertisement. Because these traditional online marketing activities mainly focus on how to invite visitors to their web sites, ROI of these marketing activities are getting lower. Many companies are looking for a new marketing method to escape this situation. In this paper, we propose ROMS (Realtime Online Marketing System) which supports tools to improve conversion ratio of e-commerce sites, ROMS gathers behavioral data of visitors and analyzes it in realtime. ROMS supports live chats, visitor profiling, context analysis, event detection, and live marketing. With ROMS, personalized offers based on visitors' realtime context can be made for each visitor.

Cholesterol Content and Formation of Cholesterol oxidative products(COPs) in Processed Squids (가열조건에 따른 오징어 Cholesterol과 Cholesterol oxidative products(COPs)의 함량변화에 관한 연구)

  • An, Duek-Jun;Hong, Jeong-Hoon
    • Journal of the Korean Society of Food Culture
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    • v.17 no.4
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    • pp.465-472
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    • 2002
  • The effect of cooking(boiling, steaming and baking) and drying on the cholesterol content and formation of oxidized cholesterols and acid value in squid(Japanese flying squid, Todarodes pacificus) was studied. Cholesterol content of live squid meat varied with the portion sampled. The data from spectophotometric assay ranged from 263.2 mg/100g(mantle) to 315.8 mg/100g(tentacle). The cholesterol levels found for squid samples analyzed by gas chromatography(GC) were lower by 7% of total cholesterol for live squid meat and 24% for processed meat than those results by spectrophotometric assay. Cooking resulted some decrease in the initial total cholesterol content of raw meat from 10%(boiling for 5 min.) to 25%(steaming for 5 min.). The amounts of cholesterol remaining after baking were 68% for microwave oven samples and 64% for convection oven samples. Drying of raw tissue caused the greater reduction in cholesterol content than cooking but brought about no significant difference in samples stored for 6 weeks at $4^{\circ}C\;and\;20^{\circ}C$. Raw squid meats contained essentially no oxidized cholesterols, while the 22-hydroxychoesterol was detected in frozen meats. The additional oxidized cholesterols as cholestane-triol was indentified with 22-hydroxycholesterol in cooked samples. Sun dried meat stored at $4^{\circ}C\;and\;20^{\circ}C$ for 6 weeks had the three kinds of oxidized cholesterols such as 22-hydroxycholesterol, cholesta-3,5-dien-7-one and cholestane-triol. For the boiled and steamed squids, 10% higher acid value and 5% higher acid value respectively were observed but oven cooked samples resulted in a 50% higher acid value than raw samples. Squids had a 45% higher acid value than raw one during sundrying and presrevation at $20^{\circ}C$ but there was not a severe difference of acid value between $4^{\circ}C\;and\;20^{\circ}C$ stored samples.

Association of Leptin Polymorphism with Production, Reproduction and Plasma Glucose Level in Iranian Holstein Cows

  • Moussavi, A. Heravi;Ahouei, M.;Nassiry, M.R.;Javadmanesh, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.627-631
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    • 2006
  • The objective of this study was to evaluate the association of genetic differences in the bovine leptin gene and milk yield, reproduction, body condition score (BCS), and plasma glucose level in Iranian Holstein cows. In total, two hundred and thirty eight cows were used and genotyped for a restricted fragment length polymorphism at the leptin gene locus. Two genotypes, AA and AB, have been distinguished which have the frequencies of 0.89 and 0.11, respectively. The genotypes were distributed according to the Hardy - Weinberg equilibrium ($x^2$ = 0.733). During the first 12 wk of lactation, milk yield and composition, live weight, BCS and plasma glucose were measured in 50 cows. Data were analyzed based on a repeated measures ANOVA. During this period, milk yield and composition, live weight, BCS and plasma glucose level were similar among the genotypes. The first cumulative 60-d milk yield, 305-d milk yield, days to first breeding, days open and days from first breeding to conception using previous lactation records were also analyzed using Standard Least Square within mixed models. Fixed effects were year, season, parity and age at calving, and sire. For the reproductive traits the cumulative first 60-d milk yield was also added to the model. Animal was fitted as a random effect. A significant association was detected between the RFLP-AB genotype and 305-d milk yield (p<0.05). The first 60-d cumulative milk yield was similar for the two genotypes (p = 0.21) and tended to be higher in the heterozygous cows. The heterozygous genotypes at the above mentioned locus had a trend to better reproductive performance than the homozygous. The results demonstrate that the RFLP B-allele can yield a higher 305-d milk production with a trend to better reproductive performance.

A Study on Life Experiences of Unmarried Mothers Residing in Shelter (시설거주 양육미혼모 삶의 경험에 관한연구)

  • Lee, Jeoung-Sil
    • The Journal of the Korea Contents Association
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    • v.20 no.7
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    • pp.636-652
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    • 2020
  • Purpose: This study aimed to have an in-depth understanding of the structure and essence of the living experiences of unmarried mothers institutionalized after childbirth through phenomenological research. Methods: The data were collected through in-depth interview with 9 unmarried mothers from October 1, 2016 to February 2017 and this is qualitative research using Colazzi's phenomenological research method. Results: The living experiences of unmarried mothers institutionalized after childbirth had 15 themes in 4 theme clusters: 'childbirth and parenting,' 'living in women's refuge,' 'becoming independent as proud mother,' and 'disentangled thread.' Conclusion: After childbirth, unmarried mothers experienced psychological pressure from the need to cope with lonely and wearing reality by themselves but struggled to overcome the reality together with their child. Unmarried mothers hoped to live the life they chose proudly and happily together with their child. It is therefore necessary to give residential and environmental support to unmarried mothers in pursuit of their stable living and to change the awareness in the whole society with the aim of removing negative social attitude and discrimination, accepting differences, and living together.

A Study on The Personalized Seamless Smart Home Service Design for Life-style Care in Phono Sapience era (Personalized Seamless 라이프스타일 케어 스마트홈 서비스디자인 연구 : 포노 사피엔스 시대를 중심으로)

  • Park, Ui Jeong;Kim, Jung Woo;Choi, Jae Boong
    • Journal of Information Technology Services
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    • v.19 no.5
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    • pp.1-14
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    • 2020
  • Mankind has been attempting to live a happy and safe family life in a residential space. Due to the advent of the mobile phone in the 1990s and the smart phone in the 2000s, when the information and communication age came, human life has been innovatively changed. The revolution of human civilization led to the Neolithic Revolution and the Iron Age, followed by a smart phone revolutionizing human life, and the revolution faces with the era of info-communication, smart phones became a daily life and the fourth industrial revolution. The fourth industrial revolution is an era of info-communication technology (ICT), creating a new paradigm across human life through technological developments such as artificial intelligence (AI), IoT, big data, mobile, and cloud. The smart home is actively researched in a direction to support the overall human life as a representative future residential culture paradigm. However, the study considering the needs according to the lifestyle, functional characteristics of each living space and human lifestyle of the Phono Sapiens era where smart phones live like daily life was relatively insufficient. In addition, research on smart home service design should be considered from the apartment residential space planning stage. Therefore, this study has significance in suggesting the direction of research on human-centered smart home service design considering the characteristics of each living space and resident's life-style in the smart phone era.