• Bulletin of the Korean Chemical Society
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• v.34 no.10
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• pp.2973-2977
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• 2013

In this study, we developed a liquid crystal (LC)-based optical sensor for monitoring enzymatic activity through orientational changes in liquid crystals (LCs) coupled to the properties of a poly-${\small{L}}$-lysine (PLL)-based polymeric membrane. We prepared a PLL-based polymeric membrane at the planar interface between the thermotropic liquid crystal and aqueous phases. The PLL-based polymeric membrane was obtained by contacting the PLL solution with water immiscible LCs, 4-cyano-4'-pentyl-biphenyl (5CB) doped with adipoyl chloride. We then investigated the membrane properties by examining the permeability of the membrane to phospholipids, 1,2-didodecanoyl-rac-glycero-3-phosphocholine (DLPC). The permeability of the membrane to transport phospholipids was monitored through the orientational transition of 5CB in contact with the dispersions of DLPC. Since trypsin can enzymatically catalyze the hydrolysis of PLL, we incubated an aqueous trypsin solution with the membrane for 2 h at room temperature to cause an increase in the permeability of the polymeric membrane to DLPC. As a result, a bright to dark optical shift of LCs was observed, which implied that an enzymatic reaction between trypsin and PLL-based membrane occurred. Two control experiments using chymotrypsin and bovine serum albumin (BSA) revealed no sign of improved permeability based on the orientational transition of LCs.

• Applied Chemistry for Engineering
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• v.8 no.3
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• pp.473-481
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• 1997

Enhanced videomicroscopy was used to observe the dynamic behavior which occurred when water containing pure nonionic surfactant was carefully contacted with equal volumes of polar oils such as oleyl alcohol and oleic acid at various temperatures. A key component of the system is a vertical-stage microscope which provides for stable interfaces by locating the oil above the denser aqueous phase. This arrangement allowed intermediate phases formed at the surface of contact to be clearly observed, as well as any spontaneous emulsification which developed. Contacting experiments with $C_{12}E_5$ as the surfactant and with pure oleyl alcohol and oleic acid soils showed little activity below the cloud point but vigorous activity at higher temperatures including formation of an intermediate lamellar liquid crystalline phase. Diffusion path theory, which allows prediction of spontaneous emulsification resulting from diffusion and of intermediate phase formation during contacting processes, was used to understand the dynamic behavior seen during contacting experiments. Tentative diffusion paths for the contacting experiments with pure oleyl alcohol were presented with the aid of a partial phase diagram of the oleyl alcohol-water-$C_{12}E_5$ system.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1262-1266
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• 2010

In this study, we investigated the use of liquid crystals to selectively detect the activity of enzymes at interfaces decorated with oligopeptide-based membranes. We prepared a mixed monolayer of tetra(ethylene glycol)-terminated lipids and carboxylic acid-terminated lipids at the aqueous-liquid crystal (LC) interface. The 17 amino-acid oligopeptide SNFKTIYDEANQFATYK was then immobilized onto this mixed monolayer through N-hydroxysuccinimide-activation of the carboxylic acid groups. We examined the orientational behavior of nematic 4-cyano-4'-pentylbiphenyl (5CB) after conjugation of the 17 amino-acid oligopeptide with the mixed monolayer assembled at the interface. Immobilization of the oligopeptide caused orientational transitions in 5CB, with a change from homeotropic (perpendicular) to tilted alignment, which was primarily due to the reorganization of the monolayer. The orientation of the 5CB molecules returned to its homeotropic state after contacting the interface containing ${\alpha}$-chymotrypsin, which can cleave the immobilized oligopeptide. Control experiments confirmed that the enzymatic activity of ${\alpha}$-chymotrypsin triggered the ordering transitions in the LC. These results suggest that the LC can provide a facile method for selective detection of enzymatic activity.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.3
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• pp.207-215
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• 2015

This paper deals with a theoretical method to calculate natural frequencies of a fixed-free rectangular tank partially in contact with an outer water gap. Orthogonal polynomials satisfying the boundary conditions of the tank are used as admissible functions in the Rayleigh-Ritz method. A quarter model of the liquid-coupled system is constructed and it is simplified to a line supported flat plate in contact with the liquid. The liquid displacement potential functions satisfying the Laplace equation and water boundary conditions are derived, and the finite Fourier transform is accomplished in conjunction with the compatibility requirement along the contacting interfaces between the tank and water. An eigenvalue problem is derived so that the natural frequencies of the wet rectangular tank can be extracted. The predictions from the proposed analytical method show good agreement with the finite element analysis results.

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.371-377
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• 2014

Characteristics of holdup and flow behavior of fluidized solid particles were investigated in a liquid-solid circulating fluidized bed ($0.102m{\times}3.5m$). Effects of liquid velocity ($U_L$), particle size ($d_P$) and solid circulation rate ($G_S$) on the solid holdup, overall particle rising velocity, slip velocity between liquid and particles and hydrodynamic energy dissipation rate in the riser were examined. The particle holdup increased with increasing $d_P$ or $G_S$ but decreased with increasing $U_L$. The overall particle rising velocity increased with increasing $U_L$ or $G_S$ but decreased with increasing $d_P$. The slip velocity increased with increasing $U_L$ or $d_P$ but did not change considerably with $G_S$. The energy dissipation rate, which was found to be closely related to the contacting frequency of micro eddies, increased with increasing $d_P$, $G_S$ or $U_L$. The solid particle holdup was well correlated with operating variables such as $U_L$, $d_P$ and $G_S$.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.1
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• pp.123-130
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• 2008

An analytical method for the free vibration of a flexible rectangular plate in contact with water is developed by the Rayleigh-Ritz method. The plate clamped along the edges is partially contacted with water at both sides. It is assumed that the contained water is incompressible and inviscid. The wet mode shape of the plate is assumed as a combination of the dry mode shapes of a clamped beam. The liquid motion is described by using the liquid displacement potential and determined by using the compatibility conditions along the liquid interface with the plate. Minimizing the Rayleigh quotient based on the energy conservation gives an eigenvalue problem. It is found that the theoretical results can predict excellently the fluid-coupled natural frequencies comparing with the finite element analysis result.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.6
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• pp.2147-2159
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• 1991

The effects of principal dimensions of internal mixing twin-fluid atomized and operating conditions on the atomizing characteristics are experimentally investigated. The tests are conducted over the wide range of air/liquid ratio to predict influences of the diameter and length of nozzle, contacting angle between air and liquid in the mixing chamber, and air orifice diameter on the mean drop size(SMD), spray angle, distribution of drop size, and spray dispersion, And also, initial distribution of liquid column by air stream within the mixing chamber are observed through the transparent nozzles. A He-Ne laser particle sizer(MALVERN Model 2604) was used to measure the Sauter.s mean diameter( $D_{321}$) and droplet sizes distribution. In this experiment the air/liquid ratio, mixing length and nozzle diameter have a great influence on SMD, spray angle, droplet sizes distribution and spray dispersion.

• Proceedings of the Membrane Society of Korea Conference
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• 1995.10a
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• pp.43-44
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• 1995

The diffusion behavior of liquid into polymer has been described by Fick's law, but the departure from Fickian diffusion is frequently found. In this study, 'noble' expressions for the rates of relaxation and sorption are introduced to eliminate these limitations. The ralaxation-sorption coupled mechanism model are based on the possibility of contacting liquid molecule and the active site which has the numerical concept of free volume. The concept has an analogy of reaction rate expressed by the possibility of collision with molecules and used in adsorption and reactive extraction etc. The new model simulated by Rungc-Kutta method for initial-value problem and Fickian diffusion is caompared with experimental data. The results show that the ralaxation-sorption coupled mechanism is able to account well for Fickian and non-Fickian sorption behavior including sigmoid and two-stage. In addition, this model has a chance of expansion to multi-component sorption with ease.

• Journal of Hydrogen and New Energy
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• v.35 no.4
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• pp.410-414
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• 2024

In this paper, scrubber modeling and optimization works have been performed for the removal of ethanol contained in the feed nitrogen gas. Ethanol content at scrubber top gas stream was reduced to 20 ppm in mole by contacting counter-currently with water as a solvent. Some of the liquid withdrawn at the scrubber bottom stream has been recycled to the scrubber in order to reduce the amount of waste water.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.4
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• pp.92-102
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• 2003

To detect electrical faults of a TFT (Thin Film Transistor) panel for the LCD (Liquid Crystal Display), techniques of converting electric field to an image are used One of them is the PDLC (polymer-dispersed liquid crystal) modulator which changes light transmittance under electric field. The advantage of PDLC modulator in the electric field detection is that it can be used without physically contacting the TFT panel surface. Specific pattern signals are applied to the data and gate electrodes of the panel to charge the pixel electrodes and the image sensor detects the change of transmittance of PDLC positioned in proximity distance above the pixel electrodes. The image represents the status of electric field reflected on the PDLC so that the characteristic of the PDLC itself plays an important role to accurately quantify the defects of TFT panel. In this paper, the image of the PDLC modulator caused by the change of electric field of the pixel electrodes on the TFT panel is acquired and how the characteristics of PDLC reflect the change of electric field to the image is analyzed. When the holding time of PDLC is short, better contrast of electric field image can be obtained by changing the instance of applying the driving voltage to the PDLC.