• 한국수정란이식학회지
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• 제24권3호
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• pp.199-205
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• 2009

This study was carried out to investigate the effective genetic resources preservation system using the frozen boar semen. The porcine oocytes were matured for 44 hours in NCSU-23 medium with or without 10% Porcine Follicle Fluid (PFF), 0.5 ${\mu}g/ml$ porcine FSH, 0.5 ${\mu}g/ml$ equine LH, 1.0 ${\mu}g/ml$ 17 $\beta$-estradiol ($E_2$) and 10 ng/ml Epidermal Growth Factor (EGF) under mineral oil at $38.5^{\circ}C$ in humidified atmosphere of 5% $CO_2$ in air. After 44 h of culture, the oocytes were inseminated with frozen-thawed semen and fresh semen prepared with mTBM medium for 6 h. Later, set of 50 presumptive zygotes were transferred into 4-well dish (500 ${\mu}l$) of IVC medium. for embryos freezing, slow-freezing and vitrification methods were used as a cryopreservation. Differences among treatments were analyzed using General Linear Model Procedure by SAS Package (version 6.12) differences were considered significant when p<0.05. Following IVF and IVC, the rates of cleavage and blastocysts formation were significantly higher (p<0.05) in hormone supplemented group than that of hormone-free group (25.7 vs, 12.1). The development rates to cleavage and blastocysts were significantly higher in PZM-5 group than NCSU-23 group (60.3%, 46.6% vs 27.4%, 11.1%). Further improvement was achieved when PZM-5 was supplemented with FBS. Cleavage rates was significantly higher in fresh semen source group than frozen semen (66.7% vs 43.7%). However in blastocysts rates was similar two groups. Post-thaw survival rates of embryos were 1.2% and 2.2% in slow-frezing and vitrification groups, respectively. The results of our study suggest that it is still possible to improve the culture conditions and boar semen cryopreservation for enhance reproductive technology and animal genetic resources conservation.

• 한국치위생학회지
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• 제11권6호
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• pp.833-841
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• 2011

Objectives : The purpose of this study was investigation of quality and quantity of nutritional intake related oral health status among Korean elders. Methods : The nutrient intake and the dietary quality was evaluated on the basis of the Dietary Reference Intakes For Koreans(KDRIs). Chi-square test for Complex Samples was used to determine the relationship between oral health and inadequate nutrient intake in Korean elders. The complex samples general linear model was used to test difference of average value difference of nutrient intake percentage compared to dietary reference intake(DRI), energy intake rate from three major nutrients, average mean adequacy ratio(MAR) and index of nutritional quality(INQ) related oral health status. Age, sex and total energy intake was compensated for this analysis. PASW 18 was used for statistical analysis. Results : We could found the difference of the nutrient intake and the dietary quality related oral health status among Korean elders. Especially, Nutrient intake percentage and component ratio of protein among energy intake rate from three major nutrient was lower as oral health status became worse. The percentage of subjects with nutritional intakes under showed highest level in worst oral health status. As oral health status became worse, average mean adequacy ratio(MAR) was lower and the number of nutrient of which index of nutritional quality(INQ) was under 1 was more. Conclusions : From the result above, this study clearly shows the level of oral health affecting the inequalities of eating and the food for the people. And the various propose of oral health policies is needed for vulnerable groups who needs solution to solve the problem of inequality of food distribution where intensive distribution of nutrition problem occurred. Sufficient, safe, and a variety of healthy food intake is a fundamental right of our people. And also, to apply this policy in reality, institutional arrangements and organizations, and specific performing system will be needed.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.620-626
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• 2004

For general nonlinear processes, it is difficult to control with a linear model-based control method and nonlinear controls are considered. Among the numerous approaches suggested, the most rigorous approach is to use dynamic optimization. Many general engineering problems like control, scheduling, planning etc. are expressed by functional optimization problem and most of them can be changed into dynamic programming (DP) problems. However the DP problems are used in just few cases because as the size of the problem grows, the dynamic programming approach is suffered from the burden of calculation which is called as 'curse of dimensionality'. In order to avoid this problem, the Neuro-Dynamic Programming (NDP) approach is proposed by Bertsekas and Tsitsiklis (1996). To get the solution of seriously nonlinear process control, the interest in NDP approach is enlarged and NDP algorithm is applied to diverse areas such as retailing, finance, inventory management, communication networks, etc. and it has been extended to chemical engineering parts. In the NDP approach, we select the optimal control input policy to minimize the value of cost which is calculated by the sum of current stage cost and future stages cost starting from the next state. The cost value is related with a weight square sum of error and input movement. During the calculation of optimal input policy, if the approximate cost function by using simulation data is utilized with Bellman iteration, the burden of calculation can be relieved and the curse of dimensionality problem of DP can be overcome. It is very important issue how to construct the cost-to-go function which has a good approximate performance. The neural network is one of the eager learning methods and it works as a global approximator to cost-to-go function. In this algorithm, the training of neural network is important and difficult part, and it gives significant effect on the performance of control. To avoid the difficulty in neural network training, the lazy learning method like k-nearest neighbor method can be exploited. The training is unnecessary for this method but requires more computation time and greater data storage. The pH neutralization process has long been taken as a representative benchmark problem of nonlin ar chemical process control due to its nonlinearity and time-varying nature. In this study, the NDP algorithm was applied to pH neutralization process. At first, the pH neutralization process control to use NDP algorithm was performed through simulations with various approximators. The global and local approximators are used for NDP calculation. After that, the verification of NDP in real system was made by pH neutralization experiment. The control results by NDP algorithm was compared with those by the PI controller which is traditionally used, in both simulations and experiments. From the comparison of results, the control by NDP algorithm showed faster and better control performance than PI controller. In addition to that, the control by NDP algorithm showed the good results when it applied to the cases with disturbances and multiple set point changes.

• 한국해안해양공학회지
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• 제12권3호
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• pp.139-148
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• 2000

본 연구에서는 유체가 채워진 착저식 유연막 구조물과 파와의 상호작용 문제를 유탄성 이론을 사용하여 살펴보았다. 먼저 동역학적 문제를 풀기에 앞서 유체로 채워진 유연막 내부에 일정한 압력이 작용하였을 때, 유연막의 형상과 막에 작용하는 초기장력을 정역학문제를 풀어 구한다. 동역학적 문제를 풀기 위하여 유체영역을 내부영역과 외부영역으로 나누어, 내부영역을 유연막을 경계로 영역 1과 영역 2로 다시 나눈다. 내부영역에서는 경계요소법을 사용하여 파동장을 풀고, 외부영역에서는 고유함수전개법을 사용하여 해를 구한다. 두 영역이 만나는 정합면에서 이미 구한 해를 정합시켜 완전한 해를 구한다. 유연막의 거동은 원주 좌표계를 사용하여 유도된 선형화된 막방정식을 사용하여 이때 외력은 영역 1과 영역 2의 압력차로 주어지므로 영역 1과 영역 2의 해는 막방정식을 통하여 연성된다. 유체가 채워진 착저식 유연막 방파제의 성능에 미치는 중요한 변수로는 유연막의 형상(폭, 높이)과 유연막 내부압력, 유체의 밀도이다. 설계변수들을 바꿔가면서 유연막에 의한 파랑제어 효과를 투과율을 통하여 살펴보았다. 또한 파의 입사각도에 따른 파랑제어 효과를 함께 고찰하였다. 수치계산결과는 Ohyama의 실험결과와 비교하였고 두 결과는 정량적인 값 차이가 나지만 정성적으로 일치하고 있음을 확인하였다. 적절히 설계된 유연막은 소형어항 보호용이나 레저용 방파제로 활용할 수 있는 가능성을 발견하였다.

• 한국수자원학회논문집
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• 제41권11호
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• pp.1095-1106
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• 2008

기후변화와 지구온난화현상은 지구 전체에 걸쳐 분명하게 나타나고 있으며 그에 따라 발생할 수 있는 수문 변화에 대한 연구가 다양하게 이루어지고 있다. 본 연구에서는 기후변화에 따른 유역 유출의 민감도를 평가하기 위하여 SWAT 모형을 이용하였으며 대청댐유역에 적용하였다. 모형의 보정은 1982-1995년의 월평균 하천유량을 이용하였고 1996-2005년의 자료를 이용하여 검증하였다. 기후변화에 따른 수문 변동을 정량적으로 분석하기 위하여 1988-2002년을 기준시나리오 기간으로 설정하였으며 이산화탄소 농도, 기온, 강수의 변화에 따른 총 7개의 시나리오를 구성하였다. 7개의 시나리오 중 배증 이산화탄소와 기온의 5.4℃ 증가를 반영하는 시나리오는 연평균 4～5%의 하천유량 증가를 예측하였고, 강수량의 변화를 반영하는 시나리오는 -42, -17, 17, 42%의 변화에 따라 -55, -24, 25, 64%의 비선형적인 하천유량 증감이 예측되었다. 기후인자의 변화를 복합적으로 반영하고 있는 시나리오에서는 평균 12%(봄, 여름, 가을)와 63%(겨울)의 하천유량 변화를 예측하였다. 또한 유황분석 결과 대청댐유역의 유출은 기후변화에 매우 민감한 것으로 나타났으며, 이러한 기후변화는 가뭄과 홍수의 심도와 발생기간에 큰 영향을 미칠 것으로 판단된다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.4101-4101
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• 2001

Information of body composition (fat and protein) in living animal is important to determine the nutrients requirement. Deuterium oxide (D2O) dilution techniques, as one of isotope dilution techniques have been useful for the prediction of body composition. However, the determination of D2O concentration is time consuming and complicated. Therefore this study was conducted to develop a new method to predict D2O concentration in plasma using near infrared spectroscopy technique (NIRS). Four dairy cows in early lactation were used. They were fed total mixed ration containing conr silage, timothy hay, and concentrates to make 17.0%CP and 14.0 MJDE/kgDM. Dosing D2O was at week 1,3 and 5 after parturition. After dosing D2O, the blood was collected from hour 0 to 72. Blood samples were then centrifuge at 3,000 rpm for 10 minutes to obtain plasma. D2O concentration was analyzed by gas chromatograph (deuterium oxide analyzable system, HK102, Shokotsusyou) after extracted from plasma by liophilization. Plasma sample was scanned by NIRS using Pacific Scientific (Neotec) model 6500 (Perstorp Analytical, Silver Spring, MD) in the range of wavelength from 1100 to 2500 nm. Calibration equation was developed using multiple linear regression. Sample from one animal (cow #550; n: 74) was used for developing the calibration while the rest three animals were used for validating the equation. The range, R and SEC of the calibration set samples were 135-925 ppm, 0.93 and 48.1 ppm, respectively. Validation of the calibration equation for three individual cows was done and the average of NIR predicted value of D2O at each collection time from three weeks injection showed a high correlation. The range, r and 53 of plasma from cow #474 were 322-840 ppm,0.93 and 53.1; cow #478 were 146-951 ppm,0.95 and 39.8; cow #942 were 313-885 ppm,0.95 and 37.2, respectively. Judgement of accuracy based on ratio of standard deviation and standard error in validation set samples (RPD) for cow #474, #478 and #942 were 2.2,4.3 and 3.4, respectively. The error in application due to the variation between individual was considered smaller than the bias from collection period, however, this prediction can be overcome with correction of standard zero-minute concentration of blood. The results of this preliminary study on the use of NIRS for determination of D2O in plasma showed very promising as shown by a convenient and satisfy accuracy. Further study on various physiological stage of animal should be done.

• 대한교통학회지
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• 제36권3호
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• pp.216-228
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• 2018

본 연구는 도로 소성변형이 존재하는 구간의 기상악화시 안전성을 제고하기 위하여, 도로 소성변형자료와 기상자료, 교통량 자료를 통해 소성변형구간의 물고임 변화를 분석하였으며 이와 더불어 시간전개별 일조율 자료를 적용하여 물고임 구간의 노면상태 변화를 분석하였다. 실제 구국도 3호선 성남-장호원 구간 중 8.3km 구간의 소성변형 데이터를 수집하고 설계도면 자료의 수치 변환을 통하여 3차원 지형표면을 생성한 후 동일좌표체계의 도로선형자료를 반영하여 3차원 도로 모델링을 생성하고 보다 사실적인 도로를 묘사하기 위해 도로 위 음영이 발생할수 있는 시설물을 추가 설계하였다. 또한 본 구간의 일조율 분석을 위해 태양의 고도 및 방위각을 계산하여 설정하였으며 1시간 간격의 음영데이터를 취득하여 시간전개별 음영정도를 고려할 수 있었으며 이를 도로 증발량 산정식에 대입하여 시간전개에 따른 물고임 변화를 보다 현실적으로 파악하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.73-73
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• 2018

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• 대한전자공학회논문지TC
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• 제37권2호
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• pp.32-45
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• 2000

본 논문에서는 CDMA 기지국에 선형 어레이 안테나를 적용하고자 할 경우, beamforming(BF) 성능과 관련된 두 가지 주요지표인 주 빔 폭의 좁은 정도를 지칭하는 direction selectivity와 사용자 신호의 방향추정 정확도가 BF의 SINR(Signal to interference and noise ratio) 성능에 미치는 영향과 이들 상호간의 관련성을 해석적으로 유도하였으며, 이를 수신 동작 시뮬레이션을 통하여 확인하였다. BF에 내포된 사용자 방향추정 기능과 간섭 사용자 신호 억제기능에 대하여 이들의 오차가 SINR에 미치는 영향을 구분하여 알아보기 위한 목적으로 방향추정 기능만을 포함한 steering BF 알고리즘과 간섭 사용자 전력을 최소화시키는 기능이 포함된 MVDR(Minimum variance distortionless response) BF 알고리즘을 대상으로 해석 및 동작 시뮬레이션을 수행하였다. 이 과정에 BF 알고리즘의 방향추정 기능에 직접적으로 오차를 유발하는 전파의 공간적 scattering 현상에 대한 모델을 새로이 제시하여 적용하도록 하였다. 본 연구의 결과, 종래의 군사적 목적의 BF와는 달리 셀룰러 CDMA 기지국에 array 안테나를 적용하고자 할 경우는 direction selectivity 및 사용자 방향추정 정확도를 향상시키고자 하는 것이 구현의 경제성 및 오차요인들에 대한 robustness 측면에서는 적합하지 않다는 것을 확인하였다. 이러한 특성은 BF 성능열화에는 크게 영향을 주지 않으면서 복잡도를 최소화하고 오차요인에 대한 robustness 특성을 갖는 경제적인 BF 알고리즘의 구현을 가능케 하여주는 중요한 사실이다. 이에 본 연구에서 제시한 해석의 결과는 셀룰러 CDMA기지국에 대한 BF 알고리즘 개발 및 적용의 설계지표로서 의미가 크다하겠다.

• 한국지능시스템학회논문지
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• 제15권2호
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• pp.149-162
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• 2005

본 논문에서는 실시간 폐쇄회로 화면으로 받은 컬러 이미지에서 얼굴영상을 추출하고 이미 지정된 특정인의 얼굴영상과 비교를 통해 지하철이나 은행 등 공공장소에서의 수배자 등 어떤 특정인을 검출하는 방법을 제안하고자 한다. 감시카메라의 특성상 화면속의 얼굴정보가 임의의 크기로 가변하고 영상 내에서 다수의 얼굴정보를 포함하고 있음을 가정할 때, 얼굴영역을 얼마나 정확하게 검색 할 수 있느냐에 초점을 맞추었다. 이를 해결하기 위하여F.Rosenblatt가 제안한 퍼셉트론 신경망 모델을 기초로 임의의 얼굴영상에 대한 $20{\times}20$ 픽셀로 서브샘플링을 사용한 규준화 작업을 통해서 전면얼굴에서와 같은 인식기법의 효과를 사용하고, 획득한 얼굴후보 영역에 대하여 조명이나 빛에 의한 외부환경의 간섭을 최소화하기 위하여 최적선형필터와 히스토그램 평활화 기법을 이용하였다. 그리고 불필요한 학습을 최소화하기 위하여 달걀형 마스크의 덧셈연산을 전 처리 과정에 추가하였다. 전 처리 과정을 마친 이미지는 각각 세 개의 수용필드로 쪼개어져 특정 위치에 존재하는 눈, 코, 입 능의 정보를 신경망 학습을 통해 최종 결정된다. 또한 각각 다른 초기값을 가지는 3개의 단일셋 네트워크시스템을 병력형태로 구성하여 결과의 정확도를 높여 구현하였다.