• Title/Summary/Keyword: limit pressure

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Determination of Betaine in Fructus Lycii Using Hydrophilic Interaction Liquid Chromatography with Evaporative Light Scattering Detection

  • Shin, Hyun-Du;Suh, Joon-Hyuk;Kim, Jung-Hyun;Lee, Hye-Yeon;Eom, Han-Young;Kim, Un-Yong;Yang, Dong-Hyug;Han, Sang-Beom;Youm, Jeong-Rok
    • Bulletin of the Korean Chemical Society
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    • v.33 no.2
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    • pp.553-558
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    • 2012
  • A simple new method was developed for the determination of betaine in Fructus Lycii using hydrophilic interaction liquid chromatography with evaporative light scattering detection (HILIC-ELSD). Good chromatographic separation and reasonable betaine retention was achieved on a Kinetex HILIC column ($2.1{\times}100mm$, $2.6{\mu}m$) packed with fused-core particle. The mobile phase consisted of (A) acetonitrile and (B) 10 mM ammonium formate (pH 3.0)/acetonitrile (90/10, v/v). It was used with gradient elution at a flow rate of 0.7 mL/min. The column temperature was set at $27.5^{\circ}C$ and the injection volume was $10{\mu}L$. The ELSD drift tube temperature was $50^{\circ}C$ and the nebulizing gas (nitrogen) pressure was 3.0 bar. Stachydrine, a zwitterionic compound, was used as an internal standard. Calibration curve over $10-250{\mu}g/mL$ showed good linearity ($R^2$ > 0.9992) and betaine in the 70% methanol extract of Fructus Lycii was well separated from other peaks. Intraand inter-day precision ranged from 1.1 to 3.0% and from 2.4 to 5.3%, respectively, while intra- and inter-day accuracy ranged from 100.0 to 107.0% and from 94.3 to 103.9%, respectively. The limit of quantification (LOQ) was $10{\mu}g/mL$ and the recoveries were in the range of 98.2-102.7%. The developed HILIC-ELSD method was successfully applied to quantitatively determine the amount of betaine in fourteen Fructus Lycii samples from different locations, demonstrating that this method is simple, rapid, and suitable for the quality control of Fructus Lycii.

Behavior of Quaywall Pile by Lateral Movement of Revetment on Soft Ground (연약지반 호안의 측방유동에 따른 안벽 말뚝의 거동)

  • Shin, Eunchul;Park, Jeongjun;Ryu, Ingi
    • Journal of the Korean GEO-environmental Society
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    • v.7 no.4
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    • pp.53-62
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    • 2006
  • Recently, the lateral displacement of the passive piles which are installed under the revetment on the soft ground is very important during the land reclamation work along the coastal line. The revetment on the soft clay develops the lateral displacement of ground when the revetment loading exceeds a certain limit. The lateral displacement of ground causes an excessive deformation of underground structure itself and develops lateral earth pressure against the pile foundation. The subject of study is to investigate the lateral displacement of pile foundation during the construction of container terminal at the ${\bigcirc}{\bigcirc}{\bigcirc}{\bigcirc}$ port in Incheon. The displacement of pile and the vertical settlement were measured in the field and finite element method(FEM) analysis for each construction sequence was performed using AFFIMEX(Ver 3.4). From the comparison of the results from field measurement and the finite element analysis, the settlement of the reventment has already occurred at the time of field measurements. Since then, the noticeable lateral displacement of piles and settlement were occurred during the filling of dredged soil inside the revetment dredging and reclaiming work. After completing filling, the lateral displacement and field settlement were reduced remarkably. Generally, the results from the finite element analysis show larger than those from the measurement.

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Unguided Rocket Trajectory Analysis under Rotor Wake and External Wind (로터 후류와 외풍에 따른 무유도 로켓 궤적 변화 해석)

  • Kim, Hyeongseok;Chae, Sanghyun;Yee, Kwanjung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.1
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    • pp.41-51
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    • 2018
  • Downwash from helicopter rotor blades and external winds from various maneuvering make an unguided rocket change its trajectory and range. For the prediction of the trajectory and range, it is essential to consider the downwash effect. In this study, an algorithm was developed to calculate 6-Degree-Of-Freedom(6 DOF) forces and moments exerting on the rocket, and total flight trajectory of a 2.75-inch unguided rocket in a helicopter downwash flow field. Using Actuator Disk Model(ADM) analysis result, the algorithm could analyze the entire trajectory in various initial launch condition such as launch angle, launch velocity, and external wind. The algorithm that considered the interference between a fuselage and external winds could predict the trajectory change more precisely than inflow model analysis. Using the developed algorithm, the attitude and trajectory change mechanism by the downwash effect were investigated analyzing the effective angle of attack change and characteristics of pitching stability of the unguided rocket. Also, the trajectory and range changes were analyzed by considering the downwash effect with external winds. As a result, it was concluded that the key factors of the rocket range change were downwash area and magnitude which effect on the rocket, and the secondary factors were the dynamic pressure of the rocket and the interference between a fuselage and external winds. In tailwind case which was much influential on the range characteristics than other wind cases, the range of the rocket rose as increasing the tailwind velocity. However, there was a limit that the range of the rocket did not increase more than the specific tailwind velocity.

Analysis on the Stress of Hydraulic Cylinder for Large Vessel by Boundary Element Method (대형선박용 유압실린더에서 경제요소법을 이용한 응력해석)

  • 김옥삼
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.4
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    • pp.423-434
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    • 1995
  • It was used boundary element method(BEM) and analysed axisymmetric problem to solve hydraulic cylinder for large vessel acting uniform internal pressure(25N/m super(2)) within elastic limit. This paper was utilized the carbon steel tubes for machine structural purposed model, inner radius was 150mm and outer radius was 250mm, axial length was semi-infinite and the isoparametric element was used. The important results obtained in this study were summarized as follows. Radial, tangential and shearing stress occured the maximum stresses(48, -20 and 34MPa) at the inner radius and the minimum stresses(32, -4 and 18MPa) at the outer radius of the hydraulic cylinder for large vessel. But negative signs have meaning compressive stress and stress diminution ratio was about 0.15MPa/mm. The use of isoparametric element raised accuracy and the increment of input data lessened the error in internal point but computer run-time was increased. The double node was improved the internal solutions to settle discontinuity at corner and the double exponential formula lessened error of stress value at boundary neighborhood. And then coincidence between the analytical and exact results is found to be fairly good, showing that the proposed analytical by BEM is reliable.

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Effect of L-Glutamic Acid and Paraben Solution on the Endothelial Cell Proliferation in the Glutaraldehyde- Fixed Bovine Pericardium (글루타르알데하이드 고정 소심 낭막에서의 내피세포 증식에 대한 글루탕산 및 파라벤용액의 효과)

  • Kim, Beom-Sik;Lee, Mun-Hwan;Yu, Se-Yeong;Kim, Won-Gon
    • Journal of Chest Surgery
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    • v.29 no.1
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    • pp.7-13
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    • 1996
  • The conventional glutaraldehyde (GA) fixation method of tissue valves is considered to be responsible for accelerated valve degeneration. The release of toxic GA from the valve tissue is believed to limit endothelial cell (EC) ingrowth. Removal of toxic GA by reaction with L-glutamic acid and storage in a Paraben solution may offer good EC growth. To investigate the conditions for endothelialization of tissue valves, the growth properties of ECs on the conventionally and alternatively treated pericardial tissue were compared. Conventional preparation included zero-pressure fixation for 72 hours in phosphated-buffered saline (PBS) solution containing 0.5% GA at 4$^{\circ}C$ and storage into PBS containing 0.2% GA(group I). Alternatively treated pericardial tissues were divided into three postfixation treatment groups : (1) storage in PBS solution containing Paraben(group II), (2) treatment with PBS containing 8$^{\circ}C$ L-glutamic acid(PH 7.35) and storage in PBS solution containing Paraben (g oup III), (3) treatment with L-glutamic acid dissolved in distilled water (PH 3.5) (group IV). Pericardial tissue were transferred into the 24-well plate after storage for 4 weeks. ECs were harvested enzymatically from the bovine pulmonary artery and grown to confluence on culture flask surfaces. Detached ECs by trypsin were incubated into the each well of the 24-well plate including test pericardial tissues. Cells were detached by trypsin, 1, 2, 3, 5, 7 days after incubation and counted on the hemacytometer. Cell viability test was performed by frypan-blue exclusion method. Acute cell death in the group I were found even after prolonged washing. The group II showed prolonged cell survival compared with the group I. Both group III and group IV showed better cell growth than group II. There was no statistically significant difference between group III and group IV method in terms of EC growth. This results suggest that treatment by L-glutamic ac id and storage in a Paraben solution be a promising approach for improvement of durability of GA-treated tissue valves.

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FMEA of Electrostatic Precipitator for Preventive Maintenance (전기집진기 예지보전 단계에서의 고장모드영향분석)

  • Han, Seung-Hun;Lee, Jeong-Uk;Lee, Sun-Youp;Hwang, Jong-Deok;Kang, Dae-Kon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.6
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    • pp.706-714
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    • 2020
  • Currently, 90 % of the world's population breathes air with a fine dust content exceeding the World Health Organization's annual average exposure limit (10 ㎍/㎥). Global efforts have been devoted toward reducing secondary pollutants and ultra-fine dust through regulations on nitrogen oxides released over land and sea. Domestic efforts have also aimed at creating clean marine environments by reducing sulfur emissions, which are the primary cause of dust accumulation in ships, through developing and distributing environment-friendly ships. Among the technologies for reducing harmful emissions from diesel engines, electrostatic precipitator offer several advantages such as a low pressure loss, high dust collection efficiency, and NOx removal and maintenance. This study aims to increase the durability of a ship by improving equipment quality through failure mode effects analysis for the preventive maintenance of an electrostatic precipitator that was developed for reducing fine dust particles emitted from the 2,427 kW marine diesel engines in ships with a gross tonnage of 999 tons. With regard to risk priority, failure mode 241 (poor dust capture efficiency) was the highest, with an RPN of 180. It was necessary to determine the high-risk failure mode in the collecting electrode and manage it intensively. This was caused by clearance defects, owing to vibrations and consequent pin loosening. Given that pin loosening is mainly caused by vibrations generated in the hull or equipment, it is necessary to manage the position of pin loosening.

The Effect of Ankle Balance Training on Unstable and Stable Surface on Proprioception, Balance and Muscle Strength in Obese Middle-Aged Woman (불안정지지면과 안정지지면에서의 발목균형훈련이 비만중년여성의 고유수용성감각, 균형 및 근력에 미치는 영향)

  • Han, Jun Ho;Woo, Sunghee;Lee, Hyojeong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.05a
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    • pp.580-583
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    • 2018
  • Purpose: The purpose of this study was to compare the effects of ankle balance training on unstable and stable surfaces to discover on which surface the proprioception, balance and muscle strength of obese middle aged women improves more. Method: 30 obese middle-aged women were randomly recruited and divided into two groups (training on an unstable surface, training on a stable surface). The subjects in each group participated in the training for six weeks, three times per week for 30 minutes each session. Proprioception was measured using Dualer IQ digital inclinometer; sway length (SL) and sway area (SA) of center of pressure, and limit of stability (LOS) were measured for balance; muscle strength before and after the training was measured using manual muscle testing. Results: There were significant improvements in the subscales of the proprioception, balance and muscle strength in those who participated in ankle balance training on the unstable surface, and in those who participated on the stable surface. Therefore, the training of participants on unstable and stable surfaces did not identify which ankle balance training technique was more effective. Conclusion: Unstable and stable surface ankle balance training are both effective in improving the proprioception, balance and muscle strength of obese middle-aged woman.

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Case Studies on Distributed Temperature and Strain Sensing(DTSS) by using an Optical fiber (광섬유 센서를 이용한 온도 및 변형 모니터링에 대한 현장응용 사례)

  • Kim, Jung-Yul;Kim, Yoo-Sung;Lee, Sung-Uk;Min, Kyoung-Ju;Park, Dong-Su;Pang, Gi-Sung;Kim, Kang-Sik
    • Proceedings of the Korean Geotechical Society Conference
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    • 2006.03a
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    • pp.86-95
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    • 2006
  • Brillouin backscatter is a type of reflection that occurs when light is shone into an optical fibre. Brillouin reflections are very sensitive to changes in the fibre arising from external effects, such as temperature, strain and pressure. We report here several case studies on the measurement of strain using Brillouin reflections. A mechanical bending test of an I beam, deployed with both fiber optic sensors and conventional strain gauge rosettes, was performed with the aim of evaluating: (1) the capability and technical limit of the DTSS technology for strain profile sensing; (2) the reliability of strain measurement using fiber optic sensor. The average values of strains obtained from both DTSS and strain gauges (corresponding to the deflection of I beam) showed a linear relationship and an excellent one-to-one match. A practical application of DTSS technology as an early warning system for land sliding or subsidence was examined through a field test at a hillside. Extremely strong, lightweight, rugged, survivable tight-buffered cables, designed for optimal strain transfer to the fibre, were used and clamped on the subsurface at a depth of about 50cm. It was proved that DTSS measurements could detect the exact position and the progress of strain changes induced by land sliding and subsidence. We also carried out the first ever distributed dynamic strain measurement (10Hz) on the Korean Train eXpress(KTX) railway track in Daejeon, Korea. The aim was to analyse the integrity of a section of track that had recently been repaired. The Sensornet DTSS was used to monitor this 85m section of track while a KTX train passed over. In the repaired section the strain increases to levels of 90 microstrain, whereas in the section of regular track the strain is in the region of 30-50 microstrain. The results were excellent since they demonstrate that the DTSS is able to measure small, dynamic changes in strain in rails during normal operating conditions. The current 10km range of the DTSS creates a potential to monitor the integrity of large lengths of track, and especially higher risk sections such as bridges, repaired track and areas at risk of subsidence.

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Analysis of Deformation and Stability of Slope at the Wiri Region of Local Road 999 Nearby Andong, Gyeongsangbukdo in Korea. (999번 지방도로 경상북도 안동시 위리 지역의 사면 변형 및 안정성 분석)

  • 장현식;장보안
    • The Journal of Engineering Geology
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    • v.10 no.1
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    • pp.1-12
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    • 2000
  • Heaving of road and subsidence of slope took place at the Wiri region of the local highway 999 in Gyeongsangbukdo, Korea after heavy rain in the next year of construction. Although the state government had performed remedial treatments by reducing the angle and the height of the slope, deformation had never stopped. Therefore, we have preformed the analysis of deformation and stabilityof the slope. Study area consists of the Cretaceous shale, siltstone and sandstone and two faults are found. The major deformation occurred by sliding of rock mass along faults after heavy rain because not only thepore pressure at the fault plane and the unit weight of sliding mass increased, but did the shearstrength of saturated fault clay become very low. The decrease in shear strength of saturated fault clayis the major factor among the reasons for deformation. Numerical simulations using limit equilibriummodel, finite difference model and finite element model were performed for eight cross sections.Although safety factors are above 1.7 during the dry season, they become below 1.0 when groundwaterlevel raises to surface. The maximum displacement is about 15-3Ocm. However, safety factors increasedto above 2.4 and the maximum displacement is below 2.08cm after remedial treatment, Indicating thatthe slope becomes stable.

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Evaluation of the Stability of Quay Wall under the Earthquake and Tsunami (지진 및 지진해일파 작용하의 해안안벽의 안정성평가)

  • Lee, Kwang-Ho;Ha, Sun-Wook;Lee, Kui-Seop;Kim, Do-Sam;Kim, Tae-Hyung
    • Journal of the Korean Geotechnical Society
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    • v.27 no.3
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    • pp.41-54
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    • 2011
  • The present study analyzes the stability of waterfront quay wall under the combined action of earthquake and tsunami. Adopting the limit equilibrium method, the stability of waterfront quay wall is checked for both the sliding and overturning. Forces due to tsunami are compared with the proposed formula and the 3-D one-field Model for immiscible TWO-Phase flows (TWOPM-3D). Variations of the stability of wall are also proposed by the parametric study including tsunami water height, horizontal seismic acceleration coefficient, internal friction angle of soil, friction angle between the wall and the soil and the pore water pressure ratio. The present study about the stability of wall is also compared with the case when earthquake and tsunami are not considered. As a result, the result of numerical analysis about the tsunami force is similar to that of proposed formula. When earthquake and tsunami are simultaneously considered, the stability of wall in passive case significantly decreases and tsunami forces in active case are affected as a resistance force on the wall and so the stability of wall increases.