• Title/Summary/Keyword: lim sup

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Feeding by common heterotrophic protists on the mixotrophic dinoflagellate Ansanella granifera (Suessiaceae, Dinophyceae)

  • Hee Chang Kang;Hae Jin Jeong;An Suk Lim;Jin Hee Ok;Ji Hyun You;Sang Ah Park;Se Hee Eom
    • ALGAE
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    • 제38권1호
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    • pp.57-70
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    • 2023
  • The mortality rate of red-tide dinoflagellates owing to predation is a major parameter that affects their population dynamics. The dinoflagellates Ansanella granifera and Ansanella sp. occasionally cause red tides. To understand the interactions between common heterotrophic protists and A. granifera, we explored the feeding occurrence of nine heterotrophic protists on A. granifera and the growth and ingestion rates of the heterotrophic dinoflagellate Gyrodinium dominans on A. granifera as a function of prey concentration and those of Oxyrrhis marina at a single high prey concentration. The heterotrophic dinoflagellates Aduncodinium glandula, G. dominans, Gyrodinium moestrupii, Luciella masanensis, Oblea rotunda, O. marina, Polykrikos kofoidii, and Pfiesteria piscicida and the naked ciliate Strombidium sp. were able to feed on A. granifera. With increasing mean prey concentrations, the growth and ingestion rates of G. dominans feeding on A. granifera rapidly increased and became saturated or slowly increased. The maximum growth and ingestion rates of G. dominans on A. granifera were 0.305 d-1 and 0.42 ng C predator-1 d-1 (3.8 cells predator-1 d-1), respectively. Furthermore, the growth and ingestion rates of O. marina on A. granifera at 1,700 ng C mL-1 (15,454 cells mL-1) were 0.037 d-1 and 0.19 ng C predator-1 d-1 (1.7 cells predator-1 d-1), respectively. The growth and ingestion rates of G. dominans and O. marina feeding on A. granifera were almost the lowest among those on the dinoflagellate prey species. Therefore, G. dominans and O. marina may prefer A. granifera less than other dinoflagellate prey species. The low mortality rate of A. granifera may positively affect its bloom formation.

REGULARITY AND MULTIPLICITY OF SOLUTIONS FOR A NONLOCAL PROBLEM WITH CRITICAL SOBOLEV-HARDY NONLINEARITIES

  • Alotaibi, Sarah Rsheed Mohamed;Saoudi, Kamel
    • 대한수학회지
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    • 제57권3호
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    • pp.747-775
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    • 2020
  • In this work we investigate the nonlocal elliptic equation with critical Hardy-Sobolev exponents as follows, $$(P)\;\{(-{\Delta}_p)^su={\lambda}{\mid}u{\mid}^{q-2}u+{\frac{{\mid}u{\mid}^{p{^*_s}(t)-2}u}{{\mid}x{\mid}^t}}{\hspace{10}}in\;{\Omega},\\u=0{\hspace{217}}in\;{\mathbb{R}}^N{\backslash}{\Omega},$$ where Ω ⊂ ℝN is an open bounded domain with Lipschitz boundary, 0 < s < 1, λ > 0 is a parameter, 0 < t < sp < N, 1 < q < p < ps where $p^*_s={\frac{N_p}{N-sp}}$, $p^*_s(t)={\frac{p(N-t)}{N-sp}}$, are the fractional critical Sobolev and Hardy-Sobolev exponents respectively. The fractional p-laplacian (-∆p)su with s ∈ (0, 1) is the nonlinear nonlocal operator defined on smooth functions by $\displaystyle(-{\Delta}_p)^su(x)=2{\lim_{{\epsilon}{\searrow}0}}\int{_{{\mathbb{R}}^N{\backslash}{B_{\epsilon}}}}\;\frac{{\mid}u(x)-u(y){\mid}^{p-2}(u(x)-u(y))}{{\mid}x-y{\mid}^{N+ps}}dy$, x ∈ ℝN. The main goal of this work is to show how the usual variational methods and some analysis techniques can be extended to deal with nonlocal problems involving Sobolev and Hardy nonlinearities. We also prove that for some α ∈ (0, 1), the weak solution to the problem (P) is in C1,α(${\bar{\Omega}}$).

Structural and Electrical Properties of (La,Nd,Sr)MnO3 Ceramics for NTC Thermistor Devices

  • Shin, Kyeong-Ha;Park, Byeong-Jun;Lim, Jeong-Eun;Lee, Sam-Haeng;Lee, Myung-Gyu;Park, Joo-Seok;Lee, Sung-Gap
    • 한국전기전자재료학회논문지
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    • 제35권3호
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    • pp.292-296
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    • 2022
  • (La0.5Nd0.2Sr0.3)MnO3 specimens were prepared by a solid-state reaction. In all specimens, X-ray diffraction patterns of an orthorhombic structure were shown. The fracture surfaces of (La0.5Nd0.2Sr0.3)MnO3 specimens showed a transgranular fracture pattern be possibly due to La ions (0.122 nm) as a perovskite A-site dopant substituting for Nd ions (0.115 nm) having a small ionic radius. The full-width at half maximum (FWHM) of the Mn 2p XPS spectra showed a value greater than that [8] of the single valence state, which is believed to be due to the overlapping of Mn2+, Mn3+, and Mn4+ ions. The dependence of Mn 2p spectra on the Mn3+/Mn4+ ratio according to sintering time was not observed. Electrical resistivity resulted in the minimum value of 100.7 Ω-cm for the specimen sintered for 9 hours. All specimens show a typical negative temperature coefficient of resistance (NTCR) characteristics. In the 9-hour sintered specimen, TCR, activation energy, and B25/65-value were -1.24%/℃, 0.19 eV, and 2,445 K, respectively.

Immune Responses to Plant-Derived Recombinant Colorectal Cancer Glycoprotein EpCAM-FcK Fusion Protein in Mice

  • Lim, Chae-Yeon;Kim, Deuk-Su;Kang, Yangjoo;Lee, Ye-Rin;Kim, Kibum;Kim, Do Sun;Kim, Moon-Soo;Ko, Kisung
    • Biomolecules & Therapeutics
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    • 제30권6호
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    • pp.546-552
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    • 2022
  • Epidermal cell adhesion molecule (EpCAM) is a tumor-associated antigen (TAA), which has been considered as a cancer vaccine candidate. The EpCAM protein fused to the fragment crystallizable region of immunoglobulin G (IgG) tagged with KDEL endoplasmic reticulum (ER) retention signal (EpCAM-FcK) has been successfully expressed in transgenic tobacco (Nicotiana tabacum cv. Xanthi) and purified from the plant leaf. In this study, we investigated the ability of the plant-derived EpCAM-FcK (EpCAM-FcKP) to elicit an immune response in vivo. The animal group injected with the EpCAM-FcKP showed a higher differentiated germinal center (GC) B cell population (~9%) compared with the animal group injected with the recombinant rhEpCAM-Fc chimera (EpCAM-FcM). The animal group injected with EpCAM-FcKP (~42%) had more differentiated T follicular helper cells (Tfh) than the animal group injected with EpCAM-FcM (~7%). This study demonstrated that the plant-derived EpCAM-FcK fusion antigenic protein induced a humoral immune response in mice.

Prohexadione-calcium 처리에 따른 금잔디의 생육 조절 및 질소 흡수 억제 (Growth regulation and Nitrogen Uptake Inhibition of Zoysia matrella Applying Prohexadione Calcium)

  • 김우성;김영선;임치환
    • 한국환경농학회지
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    • 제40권4호
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    • pp.359-365
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    • 2021
  • BACKGROUND: Prohexadione-calcium (PC) is a plant growth regulator of cyclohexanedione family acting on inhibiting gibberellin biosynthesis and has been used to regulate shoot elongation of turfgrass in the golf course during the summer season. This study was conducted to investigate the effects of PC on the inhibition of shoot elongation, growth, and nitrogen uptake in Zoysia matrella. METHODS AND RESULTS: Treatments were as follows; non-treatment, control (TE 0.01 a.i. g/m2/100 mL), 0.0025PC (PC 0.0025 a.i. g/m2/100 mL), 0.005PC (PC 0.005 a.i. g/m2/100 mL), 0.01PC (PC 0.01 a.i. g/m2/100 mL) and 0.02PC (PC 0.02 a.i. g/m2/100 mL). Visual quality and chlorophyll content were not significantly different. As compared to non-treatment, shoot length and clipping yield in 0.005PC, 0.01PC and 0.02PC treatments were decreased by 29-36% and 65-82%, respectively, and those of 0.0025PC were not significantly different. N uptake of Z. matrella after applying PC was decreased by 28.9-77.8% than that of non-treatment. Inhibition effects of PC treatment in Z. matrella were not significantly different from those of control. CONCLUSION(S): These results indicated that the application of prohexadione calcium inhibited shoot elongation, clipping yield and N uptake amount in Z. matrella, and trinexapac-ethyl could be replaced with prohexadione-calcium in turfgrass management.

Investigation on structural symmetry of CsCoCl3·2H2O crystals by magic-angle spinning 1H and static 133Cs nuclear magnetic resonance

  • Park, Sang Hyeon;Jang, Du Chang;Jeon, Hara;Gyeong, Oh Yi;Lim, Ae Ran
    • 한국자기공명학회논문지
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    • 제26권1호
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    • pp.10-16
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    • 2022
  • The phase transition temperatures of CsCoCl3·2H2O crystals are investigated via differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Three endothermic peaks at temperatures of 370 K (=TC1), 390 K (=TC2), and 416 K (=TC3) were observed for phase transitions from CsCoCl3·2H2O to CsCoCl3·1.5H2O, to CsCoCl3·H2O, and then to CsCoCl3·0.5H2O, respectively. In addition, the spin-lattice relaxation time T in the rotating frame and T1 in the laboratory frame as well as changes in chemical shifts for 1H and 133Cs near TC1 were found to be temperature dependent. Our analyses results indicated that the changes of chemical shifts, T, and T1 are associated with structural phase transitions near temperature TC1. The changes of chemical shifts, T, and T1 near TC1 were associated with structural phase transitions, owing to the changes in the symmetry of the structure formed of H2O and Cs+ ions. Consequently, the structural symmetry in CsCoCl3·2H2O crystals based on temperature is discussed by the environments of their H and Cs nuclei.

스윕 가스-진공 하이브리드식 탈기막 공정을 활용한 암모니아 폐수처리 및 선택적 회수 (Ammonia Wastewater Treatment and Selective Recovery Using a Sweep Gas-Vacuum Hybrid Type Membrane Degassing Process)

  • 윤홍식;민태진;전민규;임성일;오세철;류경하;이충성;강보식
    • 한국산업융합학회 논문집
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    • 제26권6_2호
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    • pp.1171-1181
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    • 2023
  • In this study, a sweep gas - vacuum hybrid type membrane degassing process was proposed for ammonia wastewater treatment. In addition, the ammonia selective recovery of the hybrid type membrane degassing process was also investigated. As a result, the hybrid type membrane degassing process showed better degassing performance (54.9 mg NH3/m2min for 360 min) than the sweep gas type (32.3 mg NH3/m2min) or vacuum type (22 mg NH3/m2min). Additionally, the hybrid type membrane degassing process showed an excellent ammonia selectivity (103 times compared to Na+ Na+, 133 times compared to Ca2+). The ammonia selectivity was appeared to be due to the conversion characteristics of ammonium ion / dissolved ammonia depending on pH. The results in this study are expected to be used in the development of ammonia wastewater treatment and ammonia recovery in the future.

중화반응으로 증명된 Anti-Sda 1예 (A Case of Anti-Sda Proven by Urine Neutralization Test)

  • 윤승규;김장수;양진혁;윤수영;이창규;임채승;조윤정;김영기;이갑노
    • Laboratory Medicine Online
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    • 제1권1호
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    • pp.64-66
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    • 2011
  • 항-Sda는 용혈성 수혈 부작용 발생이 적은 항체이므로, 항체 선별검사에서 의심이 되어도, 항체 동정이 보통은 생략된다. 저자들은 73세 남자 환자에서 수혈 전 비예기 항체 검사에서 혼합시야 반응을 보이면서 항체 동정검사에서 확인되지 않는 항체를 확인하였다. 항-Sda 의심하에 추가적인 소변 중화반응으로 동정하였다. 비록 항-Sda는 임상적 중요성이 떨어지지만, 고빈도 항원에 대한 항체이기 때문에 교차시험 검사에서 문제가 발생할 수 있다. 항-Sda로 추정되는 경우에는 소변으로 중화된 혈청으로 검사하는 것이 부적합 교차시험에 대한 해결책이 될 수 있다.

Mucosal Immunity Related to CD8+ T Lymphocytes in Children with Helicobacter pylori Gastritis

  • Da Hee Yang;Ha Young Lee;Woohyuk Choi;Chang-Lim Hyun;Ki Soo Kang
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제27권1호
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    • pp.26-36
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    • 2024
  • Purpose: We investigated the role of CD8+T cells as host immune factors in pediatric patients with Helicobacter pylori gastritis. Methods: Gastric mucosal tissue and blood samples were collected from 39 children, including 11 children with H. pylori infection and 28 children as controls. Anti-CD8 and anti-T-bet antibodies were used for immunohistochemistry of the gastric mucosa. For the cell surface and intracellular staining, peripheral blood mononuclear cells were stained with anti-IL7Rα, anti-CX3CR1, anti-CD8, anti-T-bet, and anti-IFN-γ antibodies. Cytokines of sera such as tumor necrosis factor alpha (TNF-α) and CX3CL1 were analyzed using enzyme- linked immunosorbent assay (ELISA). Results: In the immunohistochemistry of gastric mucosa, the frequency of CD8+ and T-bet+ T cells cells was higher in the H. pylori-positive group than in the control group (26.9± 7.8% vs. 16.9±3.3%, p<0.001; 5.0±2.5% vs. 2.2±0.7%, p=0.001). Between the control and H. pylori-positive groups, the frequency of IL-7RαlowCX3CR1+ CD8+ and T-bet+ INF-γ+ CD8+ T cells were not significantly different between surface and intracellular staining, respectively (40.4±24.0% vs. 38.2±17.8%, p=0.914; 40.4±24.0% vs. 38.2±17.8%, p=0.914). In the ELISA, no significant differences in TNF-α and CX3CL1 concentrations were observed between the control and H. pylori-positive groups (34.3±12.1 pg/mL vs. 47.0±22.6 pg/mL, p=0.114/0.5± 0.1 pg/mL vs. 0.5±0.1 pg/mL, p=0.188). Conclusion: CD8+ T and Th1 cells, which secrete IFN-γ, might play important roles in the mucosal immunity of the stomach in children with H. pylori infection.