• 생약학회지
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• 제18권3호
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• pp.196-201
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• 1987

A study was carried out to investigate the possibility of acclimation of Coptis japonica Makino in Korea. Coptis japonica could be grown within 4 years as a medicinal crude drug if it is cultivated in the area of mountain facing north(300m above sea level) with 70% light interception and appropriate soil texture. It is to be noted that the propagation of the plant is more important than the yield of berberine because the berberine contents do not change in different conditions. The results were as follows; 1. The germination rates of the seeds by covering with the pine tree leaves were the highest and averaged 63%. 2. The germination rates of dry seeds were not affected in spite of low temperature and $GA_3$ treatment. 3. The germination rates of the seeds stored in moistured sand were averaged 85%, but in dry state, they were not germinated. 4. The number of leaves and fresh weight of Coptis japonica from the area of mountain facing north were increased significantly. 5. The growth and fresh weight of Coptis japonica were reduced in clay. 6. The best growth was obtained under 70% light interception. It is possible to intercrop between evergreen trees. 7. The foliage dressing did not affect the growth. 8. The number of leaves and fresh weight were increased from the 3rd year, and the average weight of rhizomes was 5.7g in 4th year. 9. The berberine contents of the rhizomes of Coptis japonica under various treatment conditions ranged from 9 to 10%. Coptidis Rhizoma from this study and from Korean market showed much higher berberine contents than those from Japanese market.

• 한국자원식물학회지
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• 제27권5호
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• pp.588-592
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• 2014

분화용 바위떡풀 신품종인 '화이트그린(whitegreen)'은 연한 녹색인 대우주(大宇宙)를 모본으로 하고 연한 노란색과 녹색이 섞인 광림(洸淋)을 부본으로 하여 2004년 인공교배에 의해 종자를 얻었다. 이후 2004년부터 2008년까지 고령지농업연구센터의 가온 온실에서 증식 및 재배를 하면서 개화특성을 검정하였다. '화이트그린'품종의 개화기간은 9월 20일부터 시작하여 10월 하순까지 약 33일간 이었으며 꽃은 385.7개로 대조품종보다 많았다. 화색은 밝은 연녹색(G-W 157B)을 띠며, 꽃잎 수는 11.4개로 겹으로 피었다. 화형은 큰대(大)자형이 아니며 화장과 화폭이 각 1.4, 1.4 cm로 대조품종보다 매우 작았다. 초장은 20.7 cm로 대조품종보다는 작았다. 엽수도 48.3개로 대조품종보다 적었다. 엽폭은 3.4 cm, 엽장은 3.0 cm로 역시 대조품종보다 작았다. '화이트그린(whitegreen)'품종은 2011년 국립종자원에 품종보호권이 등록(No. 3456)되었다.

• 식물조직배양학회지
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• 제25권5호
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• pp.347-351
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• 1998

딸기 '수홍'의 기내배양된 잎과 탁엽 절편체로부터 기관형성을 통한 식물체 재생 방법을 확립하고자 실험을 수행하였다. 엽절편체 배양에서는 생장조절제 처리에 따라 NAA 첨가시에는 뿌리 재생, BA 첨가시에는 신초 재생이 이루어졌으며, BA 1.0㎎/L + NAA 0.2 ㎎/L 처리구에서 재생률 31.1%, 절편체당 신초수 1.7개로 가장 양호하였다. 탁엽절편체 배양에서는 생장조절제와 광조건의 처리를 실시하였는데, 명조건에서는 생장조절제 처리에 따른 뚜렷한 경향을 발견할 수 없었으나, 암조건에서는 고농도 BA와 저농도 NAA의 혼용처리가 신초 재생에 필요함을 알 수 있었다. 탁엽절편체는 BA 2.0 ㎎/L + NAA 0.1㎎/L 처리구에서 재생률 44.4%, 절편체당 신초수 4.0개로 가장 양호한 반응을 나타내었다. 재생된 신초는 NAA 0.1㎎/L 가 첨가된 MS배지에서 발근되었으며, 이어서 인공토양(vermiculite : perlite = 1 : 1)에서 성공적으로 활착을 유도할 수 있었다.

• Journal of Biosystems Engineering
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• 제25권3호
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• pp.213-220
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• 2000

Grafting of fruit-bearing vegetables has been widely used to increase the resistance to soil-borne diseases, to increase the tolerance to low temperature or to soil salinity, to increase the plant vigor, and to extend the duration of economic harvest time. After grafting, it is important to control the environment around grafted seedlings for the robust joining of a scion and rootstock. Usually the shading materials and plastic films are used to keep the high relative humidity and low light intensity in greenhouse or tunnel. It is quite difficult to optimally control the environment for healing and acclimation of grafted seedlings under natural light. So the farmers or growers rely on their experience for the production of grafted seedling with high quality. If artificial light is used as a lighting source for graft-taking of grafted seedlings, the light intensity and photoperiod can be easily controlled. The purpose of this study was to develop a prototype system for the graft-taking enhancement of grafted seedlings using artificial lighting and to investigate the effect of air current speed on the distribution of air temperature and relative humidity in a graft-taking enhancement system. A prototype graft-taking system was consisted by polyurethane panels, air-conditioning unit, system controller and lighting unit. Three band fluorescent lamps (FL20SEX-D/18, Kumho Electric, Inc.) were used as a lighting source. Anemometer (Climomaster 6521, KANOMAX), T-type thermocouples and humidity sensors (CHS-UPS, TDK) were used to measure the air current speed, air temperature and relative humidity in a graft-taking system. In this system, air flow acted as a driving force for the diffusion of heat and water vapor. Air current speed, air temperature and relative humidity controlled by a programmable logic controller (UP750, Yokogawa Electric Co) and an inverter (MOSCON-G3, SAMSUNG) had an even distribution. Distribution of air temperature and relative humidity in a graft-taking enhancement system was fairly affected by air current speed. Air current speed higher than 0.1m/s was required to obtain the even distribution of environmental factors in this system. At low air current speed of 0.1m/s, the evapotranspiration rate of grafted seedlings would be suppressed and thus graft-taking would be enhanced. This system could be used to investigate the effects of air temperature, relative humidity, air current speed and light intensity on the evaportranspiration rate of grafted seedlings.

• 한국농림기상학회지
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• 제8권2호
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• pp.61-67
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• 2006

내음성정도가 서로 다른 두 수종 먼나무와 붓순나무를 대상으로 높은 광도조건인 자연상태와 낮은 광도 조건인 비음 처리구로 나누어, 두 수종간의 적응 반응을 비교하였다. 두 수종의 묘목을 처리구에서 각각 1년간 비음처리하였을 때, 엽록소함량과 광합성계, 엽육세포내 $CO_2$농도, 수분이용효율의 특성을 조사하였다. 엽록소 함량은 두 수종모두 자연조건(full sunlight)일 때, 비음처리구에 비하여 낮은 엽록소 함량들(Chl a, Chl b, Chl a+b)을 나타냈으며, 붓순나무에서 특히 자연조건과 비음 처리구간의 엽록소 함량의 차이가 크게 나타났다. 한편, 중용수인 먼나무는 PPFD $1000\;{\mu}mol\;m^{-2}s^{-1}$ 이상 일 때, 자연조건(full sunlight)에서 높은 광합성율과 높은 수분이용효율을 보였지만, PPFD $1000\;{\mu}mol\;m^{-2}s^{-1}$ 이하에서는 비음처리구에서 자연조건에 비해 더 높은 광합성율과, 수분이용효율을 나타냈다. 이에 반해 붓순나무는 모든 광도에서 비음처리를 하지 않은 묘목이 비음처리를 한 묘목에 비해 낮은 광합성율과 수분이용효율을 나타냈다. 먼나무가 탄력적으로 광도변화에 따라 유동성 있는 반응을 보이는 것에 비해 내음성이 강한 붓순나무는 광환경 변화에 따른 민감성이 낮게 나타나는 것을 알 수 있었다.

• 생물환경조절학회지
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• 제22권4호
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• pp.322-327
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• 2013

본 실험은 건전한 토마토 접목묘 생산을 목적으로 활착 및 순화기에 광환경을 달리하여 접목부위에 캘러스의 형성을 도우며, 접목묘의 광합성이 원활할 수 있는 적정 광환경을 구명하고자 수행하였다. 암 처리를 하지 않는 방법(Non), 접목부분만 암 처리하는 방법(Part), 2일간 암 처리 후 순화실로 옮기는 방법(Day-2), 4일간 암 처리 후 순화실로 옮기는 방법(Day-4) 등으로 처리하였다. 접목은 1.5mm 실리콘 튜브를 이용하는 방법으로 하였으며, 부분 암 처리를 위해 튜브를 끼워 둔 접목부분을 알루미늄호일로 감싸주었다. 연구결과, 활착율은 2일간 암 처리 후 순화실로 옮기는 방법(Day-2)에서 가장 좋았으며, 유묘의 SPAD 값과 묘소질 및 초기 수확량은 모두 접목부분만 암 처리한 방법(Part)에서 가장 우수하였다. 또한 경제성 분석에서도 부분 암처리 방법이 타 처리에 비해 추가 비용이 발생하지만, 초기 수확량이 많아서 타 처리보다 월등히 높은 소득이 예상되었다. 따라서 토마토 접목시 가장 적절한 광처리는 접목부분만 암 처리한 방법(Part)이 가장 좋은 방법으로 판단되었다.

• 수산해양교육연구
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• 제27권2호
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• pp.526-536
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• 2015

대왕바리, Epinephelus lanceolatus의 담수순화 방법에 따른 산소소비 경향을 조사하였다. 염분 변화 조건은 대조구인 35 psu (Exp. I) 외에 단계적 염분변화 조건인 Exp. II($35{\rightarrow}20{\rightarrow}10{\rightarrow}0$ psu)과 Exp. III ($35{\rightarrow}10{\rightarrow}0$ psu) 그리고 급격한 염분변화 조건인 Exp. IV ($35{\rightarrow}0$ psu)로 하였다. 염분 저하 방법별로 산소소비량을 측정한 결과, 수온 $27^{\circ}C$에서 실험어의 평균 산소소비량은 Exp. I에서 $106.9{\pm}0.7mg$ $O_2/kg/h$였다. Exp. II에서는 염분이 $35{\rightarrow}20{\rightarrow}10{\rightarrow}0$ psu로 낮아지면서 각각 $108.1{\pm}2.1$, $99.6{\pm}2.9$, $74.6{\pm}0.9$ 및 $62.7{\pm}1.0mg$ $O_2/kg/h$, Exp. III에서는 각각 $106.5{\pm}1.3$, $77.0{\pm}1.2$ 및 $64.7{\pm}1.2mg$ $O_2/kg/h$로 감소하였다. Exp. IV 역시 산소소비량은 35 psu의 $109.0{\pm}0.9mg$ $O_2/kg/h$로부터 0 psu의 $71.6{\pm}2.0mg$ $O_2/kg/h$로 감소하는 경향을 보였다. 대왕바리는 모든 실험 조건에서 암기 보다 명기에 많은 산소소비량을 보여, 주행성 어종인 것으로 추정되나, 명기와 암기의 산소소비량은 큰 차이를 보이지 않았다. 이상의 결과를 종합해 볼 때, 대왕바리 치어는 염분 저하 방법별 산소소비량에서 서로 차이를 보이지 않아 저염분 순화에 있어 생리적 문제는 발생하지 않았으며, 실험과정 중 폐사한 개체도 없었다. 따라서 대왕바리는 염분변화에 매우 강한 광염성 어종이며, 담수환경에 쉽게 적응할 수 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권3호
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• pp.336-342
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• 2009

Avian embryos at high altitude are independent of maternal protection against hypoxia, which is contrary to mammals. It is well known that chronic hypoxic exposure at key points can significantly impact on avian development. Tibetan Chicken, a Chinese indigenous breed, living in Tibetan areas with an altitude of 2.2 to 4.1 thousand meters, has an adaptive mechanism to hypoxia. In the present study, fertilized eggs of Tibetan Chicken were incubated under 13% and 21% oxygen concentration. Two lowland chicken breeds, Shouguang Chicken, an indigenous chicken breed in Shandong Province of China, and Dwarf Recessive White Chicken, an imported breed in Beijing, were used as control groups. The embryo mass and some organs such as brain, heart, liver, stomach and eye weight in the three species were measured at Hamburger-Hamilton stage 39, 41, 43 and 45 under hypoxic and normal conditions. The results showed that in hypoxia Tibetan Chicken significantly differed from the two lowland chicken breeds in embryo mass at Hamburger-Hamilton stage 41, 43 and 45 (p<0.01). In particular, Dwarf Recessive White Chicken and Shouguang Chicken showed retarded growth in hypoxic incubation (p<0.01), whereas Tibetan Chicken showed no significant difference between hypoxic and normal conditions (p>0.05). In addition, heart and the other organs showed different susceptibility to hypoxia at the studied stages. In conclusion, chronic hypoxia induced a change in the embryo development of the three different species and Tibetan Chicken showed adaptation to hypoxia. Of note, the embryo developmental physiology of Tibetan Chicken in response to hypoxia will shed light on the process of physiological acclimation or evolutionary adaptation as well as the study of clinical disease.

• 한국초지조사료학회지
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• 제37권3호
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• pp.231-236
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• 2017

Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.351-364
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• 2023

The experiment was carried out to study the effect of Korean wild ginseng adventitious root supplementation on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of Institut de selection Animale (ISA) brown laying hens at 24 weeks old. A total of 90 laying hens were subjected to a completely randomized design at three treatments, five repetitions and six laying hens per replicate. The experiments were divided by diets into the basic feed (CON), basic feed + 0.1% wild ginseng (WG1), and basic feed + 0.5% wild ginseng (WG2). The feeding trial was carried out over a duration of 12 weeks after an initial acclimation period of 2 weeks. Feeds and water were administered ad libitum in mash form, and light was available for 16 hours per day. At the end of study, henday egg production (HDEP), average egg weight (AEW), and egg mass (EM) were increased (p <0.05) in WG2 at week 12. Feed conversion ratio (FCR) was decreased (p < 0.05) in WG2 at week 12. The ginsenoside content in egg yolk was increased (p <0.05) in laying hens in the WG2 treatment at week 12. Relative expression of tumor necrosis factor alpha (TNF-α) was reduced (p < 0.05) in the WG supplemented diets at week 12. The fecal microflora quantity of Lactobacillus was increased (p < 0.05) in WG2 at week 8 to week 12, and Escherichia coli (E. coli) was significantly decreased (p < 0.05) in the WG2 at week 12. We concluded that the result observed in the HDEP, AEW, EM and FCR was due to an increase in ginsenoside content, leading to an improvement in the TNF-α, and fecal microflora quantity such as Lactobacillus and E. coli in the WG2 supplemented diets. We therefore recommend the use of WG at application level 0.5% per basal diet for optimum laying performance in layer hens.