• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.53-58
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• 2002

Adverse environmental conditions such as drought, high salt and cold/freezing are major factors that reduces crop productivity worldwide. According to a survey, 50-80% of the maximum potential yield is lost by these "environmental or abiotic stresses", which is approximately ten times higher than the loss by biotic stresses. Thus, improving stress-tolerance of crop plants is an important way to improve agricultural productivity, In order to develop such stress-tolerant crop plants, we set out to identify key stress signaling components that can be used to develop commercially viable crop varieties with enhanced stress tolerance. Our primary focus so far has been on the identification of transcription factors that regulate stress responsive gene expression, especially those involved in ABA-mediated stress response. Be sessile, plants have the unique capability to adapt themselves to the abiotic stresses. This adaptive capability is largely dependent on the plant hormone abscisic acid (ABA), whose level increases under various stress conditions, triggering adaptive response. Central to the response is ABA-regulated gene expression, which ultimately leads to physiological changes at the whole plant level. Thus, once identified, it would be possible to enhance stress tolerance of crop plants by manipulating the expression of the factors that mediate ABA-dependent stress response. Here, we present our work on the isolation and functional characterization of the transcription factors.n factors.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.285-290
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• 2020

To understand the role of small heat shock protein (sHSPs) in rice plant response to various stresses such as the heat and oxidative stresses, a cDNA encoding a 24.1 kDa mitochondrial small HSP (Oshsp24.1) was isolated from rice by rapid amplification of cDNA ends (RACE) PCR. The deduced amino acid sequence shows very high similarity with other plant small HSPs. DNA gel blot analysis suggests that the rice genome contains more than one copy of Oshsp24.1. High level of expression of Oshsp24.1 transcript was observed in rice seedlings in response to heat, methyl viologen, hydrogen peroxide, ozone, salt and heavy metal stresses. Recombinant OsHSP24.1 protein was produced in E. coli cells for biochemical assay. The protein formed oligomeric complex when incubated with Sulfo-EGS (ethylene glycol bis (succinimidyl succinate)). Our results shows that Oshsp24.1 has an important role in abiotic stress response and have potential for developing stress-tolerant plants.

• Tribology and Lubricants
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• v.36 no.1
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• pp.34-38
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• 2020

The powertrain of the waterjet system of a special-purpose vehicle makes use of the cardan shaft, which is composed of universal joints and shafts. These universal joints, composed of spiders and needle roller bearings, have to be designed with consideration for the bending and compressive stresses of the spiders and needle roller bearings, and the rating lives of the bearings. The bending and compressive stresses of the spider and bearing of a universal joint have been studied by many researchers. However, to design a universal joint effectively, overall consideration of the different specifications of needle roller bearings is necessary. In this study, the bending stresses of spiders and compressive stresses of needle roller bearings are calculated to design universal joints for powertrain cardan shafts with different roller diameters of bearing. Furthermore, the rating lives of the needle roller bearings are predicted using the calculated basic dynamic load ratings of the bearings. As a result, roller diameters less than 𝜙2.5 mm are found suitable through an analysis of the bending stress of the spider. All compressive stresses between spider and bearing, regardless of roller diameter, satisfy the requirements. Moreover, roller diameters of more than 𝜙2 mm are found suitable for the required rating life.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.39-39
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• 2003

Antioxidant enzyme genes play a key role in cell defense against the lethal effects of oxidative stresses in animals and have an essential function which has allowed the evolution of aerobic respiration starting from an ancient form of oxygen-insensitive life. Piscine antioxidant enzymes are also involved in the rapid response to various toxic chemicals as well as many biological stresses, indicating that they could be used as biomarkers for health and aquatic environment. With the purpose for developing fine molecular probing tool to assess the stresses in marine fish, we identified three major antioxidant enzyme genes (superoxide dismutase, catalase and glutathione-S-transferase) from Korean rock bream using expressed sequence tag analysis and/or high density filter screening. Here we report the molecular information on these gene transcripts including complete sequence data and expression profiles.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.1-11
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• 2017

Evolutionary studies conducted on NAC (NAM, ATAF1&2, and CUC2) genes for all major groups of land plants, indicate the presence of the NAC subfamilies, even in the early land plants. The varied roles played by NAC proteins in plant growth and development range from the formation of shoot apical meristem, floral organ development, reproduction, lateral shoot development, and defense responses to biotic and abiotic stresses. Considering the value and importance of solanaceous crops, the study of NAC proteins in these plants needs to be intensified. This will help to identify and functionally characterize their promoters, which will subsequently aid in engineering plants with improved performance under stressful conditions. In this review, the functionally characterized NAC transcription factors specific to tomato, potato, tobacco, chili pepper and eggplant (aubergine) are summarized, clearly indicating their biological functions in the defense mechanism of the plants, against biotic and abiotic stresses.

• Structural Engineering and Mechanics
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• v.61 no.3
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• pp.389-396
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• 2017

Concrete pavements are subjected to traffic and environmental loadings. Repetitive type of such loading cause fatigue distress which leads to failure by forming cracks in pavement. Fatigue life of concrete pavement is calculated from the stress ratio (i.e. the ratio of applied flexural stress to the flexural strength of concrete). For the correct estimation of fatigue life, it is necessary to determine the maximum flexural tensile stress developed for practical loading conditions. Portland cement association PCA (1984) and Indian road congress IRC 58 (2015) has given charts and tables to determine maximum edge stresses for particular loading and subgrade conditions. It is difficult to determine maximum stresses for intermediate loading and subgrade conditions. The main purpose of this study is to simplify the analysis of rigid pavement without compromising the accuracy. Equations proposed for determination of maximum flexural tensile stress of pavement are verified by finite element analysis.

• Mycobiology
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• v.37 no.3
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• pp.161-170
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• 2009

Cryptococcus neoformans is a basidiomycete human fungal pathogen that causes meningoencephalitis in both immunocompromised and immunocompetent individuals. The ability to sense and respond to diverse extracellular signals is essential for the pathogen to infect and cause disease in the host. Four major stress-activated signaling (SAS) pathways have been characterized in C. neoformans, including the HOG (high osmolarity glycerol response), PKC/Mpk1 MAPK (mitogen-activated protein kinase), calcium-dependent calcineurin, and RAS signaling pathways. The HOG pathway in C. neoformans not only controls responses to diverse environmental stresses, including osmotic shock, UV irradiation, oxidative stress, heavy metal stress, antifungal drugs, toxic metabolites, and high temperature, but also regulates ergosterol biosynthesis. The PKC(protein kinase C)/Mpk1 pathway in C. neoformans is involved in a variety of stress responses, including osmotic, oxidative, and nitrosative stresses and breaches of cell wall integrity. The $Ca^{2+}$/calmodulin- and Ras-signaling pathways also play critical roles in adaptation to certain environmental stresses, such as high temperature and sexual differentiation. Perturbation of the SAS pathways not only impairs the ability of C. neoformans to resist a variety of environmental stresses during host infection, but also affects production of virulence factors, such as capsule and melanin. A drug(s) capable of targeting signaling components of the SAS pathway will be effective for treatment of cryptococcosis.

• Nuclear Engineering and Technology
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• v.46 no.1
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• pp.101-108
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• 2014

About 40% of reactors in the world are being operated beyond design life or are approaching the end of their life cycle. During long-term operation, various degradation mechanisms occur. Fatigue caused by alternating operational stresses in terms of temperature or pressure change is an important damage mechanism in continued operation of nuclear power plants. To monitor the fatigue damage of components, Fatigue Monitoring System (FMS) has been installed. Most FMSs have used Green's Function Approach (GFA) to calculate the thermal stresses rapidly. However, if temperature-dependent material properties are used in a detailed FEM, there is a maximum peak stress discrepancy between a conventional GFA and a detailed FEM because constant material properties are used in a conventional method. Therefore, if a conventional method is used in the fatigue evaluation, thermal stresses for various operating cycles may be calculated incorrectly and it may lead to an unreliable estimation. So, in this paper, the modified GFA which can consider temperature-dependent material properties is proposed by using an artificial neural network and weight factor. To verify the proposed method, thermal stresses by the new method are compared with those by FEM. Finally, pros and cons of the new method as well as technical findings from the assessment are discussed.

• Journal of Korean Society for Quality Management
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• v.20 no.2
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• pp.21-32
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• 1992

The only property of the random stresses to affect the life length are their damage amount and are independent of the especial realization in stress case. This paper shows the result is discussed that the randomness of the life length is caused by the randomness of the strength rather than by the randomness of the stress when the load is a random function and the strength is random as well. This special model is well-suited model for comparative calculations since it connects fatigue life for random stresses to fatigue life for periodically curve loads, which is usually measured in experiments.

• Journal of Families and Better Life
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• v.20 no.6
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• pp.49-60
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• 2002

This study focused on family stresses and coping strategies among commuting couples. The participants of this study consisted of a national samples of 134 couples(268 individuals), who had been commuting for at least six months. The major findings obtained from the study are summarized in the following: First, the degree of family stresses among commuting couples was 2.98, when the maximal stress value was set to 5.0. No difference in the level of stress was detected from the roles of the wife and those of the husband. The most stressful aspect for the commuting couples came from the role of parents who care for their children. The second stressful aspect was their responsibility for their own parents, and finally the stress from the housework and maintenance of their marital lives followed next. There was no difference between the wives and husbands on the stressors. Secondly, the level of the coping strategies developed by the commuting couples themselves against their stresses was 3.37, when the maximal stress coping value was set to 5.0. Again, there was no difference in this level between wives and husbands. Among the strategies, they used ‘cognitive restructuring’the most frequently; sharing their responsibilities with other members in family life came next; and developing their own techniques to manage their stresses followed. Thirdly, the ages of the couple, the age of the first child, type of jobs, duration of the marriage and commute, and frequency of meeting affected the level of family stress among commuting couples. Finally, commuting couples developed quite different strategies to cope with their family stresses, and religion and family type affected significantly the type of coping strategies.