• 제목/요약/키워드: leaf inoculation

검색결과 313건 처리시간 0.031초

First Report of Leaf Spot in Water Spinach Caused by Ectophoma multirostrata

  • Gyo-Bin Lee;Hong-Sik Shim;Weon-Dae Cho;Wan-Gyu Kim
    • 한국균학회지
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    • 제50권4호
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    • pp.367-372
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    • 2022
  • Leaf spot symptoms were observed in water spinach (Ipomoea aquatica) plants growing in fields in Ansan and Hongseong, Korea, during disease surveys in 2019 and 2020. The symptoms appeared as brown to dark brown circular or irregular spots on the leaves of the plants. The disease incidence on the plant leaves in the fields investigated at the two locations ranged from 1% to 20%. Five single-spore isolates of Phoma sp. Were obtained from lesions of the diseased leaves. All the isolates were identified as Ectophoma multirostrata based on their cultural and morphological characteristics, as well as molecular analysis. Two isolates of E. multirostrata were tested for pathogenicity on water spinach leaves using artificial inoculation. The tested isolates caused leaf spot symptoms in the inoculated plants. These symptoms were similar to those observed in plants from the investigated fields. To our knowledge, this is the first report of E. multirostrata causing leaf spot in water spinach.

Incidence of Beet Leaf Spot Caused by Neocamarosporium betae in Korea

  • Gyo-Bin Lee;Hong-sik Shim;Weon-Dae Cho;Wan-Gyu Kim
    • 한국균학회지
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    • 제51권1호
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    • pp.63-68
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    • 2023
  • From June to August 2021, we surveyed diseases affecting beet (Beta vulgaris subsp. vulgaris) plants in Cheolwon, Hoengseong, and Pyeongchang regions in Gangwon Province, Korea. We observed severe leaf spot symptoms, such as brown to dark circular or irregular spots on the leaves, in plants. Disease incidence in the plant leaves in the fields investigated at the three locations ranged from 1 to 80%. Five single-spore isolates of Phoma sp. were obtained from the diseased leaves and identified as Neocamarosporium betae based on their cultural, morphological, and molecular characteristics. Three isolates of N. betae were subsequently tested to confirm their pathogenicity in beet plants via artificial inoculation. The tested isolates caused leaf spot symptoms in the inoculated plants, similar to those observed in the plants in the investigated fields. Therefore, our findings revealed N. betae as the pathogen causing beet leaf spot in Korea.

Didymella gigantis sp. nov. Causing Leaf Spot in Korean Angelica

  • Gyo-Bin Lee;Ki Deok Kim;Weon-Dae Cho;Wan-Gyu Kim
    • Mycobiology
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    • 제51권6호
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    • pp.393-400
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    • 2023
  • During a disease survey in October 2019, leaf spot symptoms with a yellow halo were observed on Korean angelica (Anglica gigas) plants grown in fields in Pyeongchang, Gangwon Province, Korea. Incidence of diseased leaves of the plants in the investigated fields ranged from 10% to 60%. Morphological and cultural characteristics of two single-spore isolates from the leaf lesions indicated that they belonged to the genus Didymella. Molecular phylogenetic analyses using combined sequences of LSU, ITS, TUB2, and RPB2 regions showed distinct clustering of the isolates from other Didymella species. In addition, the morphological and cultural characteristics of the isolates were somewhat different from those of closely related Didymella spp. Therefore, the novelty of the isolates was proved based on the investigations. Pathogenicity of the novel Didymella species isolates was confirmed on leaves of Korean angelica plants via artificial inoculation. This study reveals that Didymella gigantis sp. nov. causes leaf spot in Korean angelica.

Foliar Colonization and Growth Promotion of Red Pepper (Capsicum annuum L.) by Methylobacterium oryzae CBMB20

  • Lee, Min-Kyoung;Chauhan, Puneet Singh;Yim, Woo-Jong;Lee, Gyeong-Ja;Kim, Young-Sang;Park, Kee-Woong;Sa, Tong-Min
    • Journal of Applied Biological Chemistry
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    • 제54권2호
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    • pp.120-125
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    • 2011
  • In order to exploit Methylobacterium oryzae CBMB20 as of plant growth promoting agent, different inoculation methods have been evaluated. The present study aimed to evaluate soil, foliar, and soil+foliar inoculations of M. oryzae CBMB20 to improve the growth, fruit yield, and nutrient uptake of red pepper (Capsicum annuum L.) under greenhouse conditions. The population range of green fluorescent protein (gfp)-tagged M. oryzae CBMB20 using the three inoculation methods was 2.5-2.9 ${\log}_{10}$ cfu/g in the rhizosphere and 4.5-6.0 ${\log}_{10}$ cfu/g in the phyllosphere of red pepper plants. Confocal laser scanning microscopy results confirmed the colonization of M. oryzae CBMB20 endophytically on leaf surface. Plant height, fruit dry weight, and total biomass were significantly higher ($p{\leq}0.05$) in all M. oryzae CBMB20 inoculation methods as compared to non-inoculated control. Furthermore, uptake of mineral nutrients such as N, P, K, Ca, and Mg in red pepper plants in all M. oryzae CBMB20 inoculation methods was higher than in non-inoculated control. Comparative results of inoculation methods clearly demonstrated that soil+foliar inoculation of M. oryzae CBMB20 lead to the highest biomass accumulation and nutrient uptake which may be due to its efficient colonization in the red pepper rhizosphere and phyllosphere.

마늘 및 기타 Allium속 식물에 발생하는 Stemphylium vesicarium (Stemphylium vesicarium on Garlic and other Allium spp. in Korea)

  • 조혜선;유승헌
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.567-570
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    • 1998
  • More than 100 isolates of Stemphylium obtained form leaf blight lesions of garlic (Allium sativum), onion (A. cepa) and leek (A. fistulosum) were identified as Stemphylium vesicarium (Wallr.) Simm. based on various morphological characteristics rather than S. botryosum Wallr. previously reported on those plants in Korea. In controlled inoculations, the pathogen induced the disease symptoms on leaves of garlic 7 days after inoculation. Onion and leek were also artificially infected by the fungus. This is the first report of S. vesicarium causing a leaf blight of garlic, onion and leek in Korea.

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벼 도열병에 대한 품종의 지속저항성 발현요인에 관한 연구 2. 도열병에 대한 지속저항성 품종의 저항성 구성요소 (Factors Affecting the Expression of Durable Resistance of Rice Cultivars to Blast caused by Pyricularia grisea Sacc 2. Components of the Resistance of Durably Resistant Cultivars for Rice Blast)

  • 라동수;오정행;김장규
    • 한국식물병리학회지
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    • 제13권2호
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    • pp.85-89
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    • 1997
  • Etiological components of the rice cultivars possessing durable resistance to rice blast were evaluated. In the greenhouse test by artificial inoculation, number of leaf blast lesion in Dongjinbyeo was not increased by any race of the pathogen, while the lesion number on the resistant cultivar Palgongbyeo and susceptible cultivar Jinmibyeo was increased rapidly. Size of the lesion in Dongjinbyeo was smaller and progressed more slowly than the resistant cultivar Palgongbyeo until 11 days after inoculation. Latent period in Dongjinbyeo was intermediate. However, the period was significantly variable depending upon the races. Least amount of conidia was produced in Dongjinbyeo by race KJ-107 and the number of conidia was decreased rapidly from 11 days after inoculation.

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Histological and Ultrastructural Study of Susceptible and Age-related Resistance Responses of Pepper Leaves to Colletotrichum cocodes Infection

  • Hong, Jeum-Kyu;Lee, Yeon-Kyeong;Jeun, Yong-Chull;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • 제17권3호
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    • pp.128-140
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    • 2001
  • Infection of pepper leaves by Colletotrichum cocodes at the two- and eight-leaf stages caused susceptible and resistant lesions 96 h after inoculation, respectively. At the two-leaf stage, progressive symptom development occurred on the infected leaves. In contrast, localized necrotic spots were characteristic symptoms at the eight-leaf stage. Infected leaves at the two-leaf stage exhibited cell death accompanied by the accumulation of autofluorescent compounds. At the eight-leaf stage, pepper leaves infected by the anthracnose fungus displayed localized autofluorescence from the symptoms. Infection of pepper leaves by C. cocodes at the two-leaf stage resulted in its rapidand massive colonization of all the leaf tissues including the vascular tissue, together with cytoplasmic collapse, distortion of chloroplasts, and disruption of host cell walls. However, penetration of C. cocodes was very limited in the older leaf tissues of pepper plants at the eight-leaf stage. Fungal hyphae grew only in the intramural spaces of the epidermal cell walls at this stage. Occlusion of amorphous material in xylem vessels, aggregation of fibrillar material in inter-cellular spaces, and deposition of protein bodies were found as resistance responses to C. cocodes.

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Nutritional Disorders, Analytical Diagnosis and Nutrient Guide for Mulberry, Morus indica L.

  • Singhal, B.K.;Chakraborti, S.;Rajan, Mala V.;Thippeswamy, T.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제8권1호
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    • pp.1-15
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    • 2004
  • Due to recent scientific innovations in mulberry cultivation, leaf yield has been increased manifold. However, with successive leaf harvest, a quantum drop in leaf yield and quality has been noted. This inturn has affected the silkworm rearing and farmers suffered by the frequent crop failures. This is mainly due to nutrient deficiencies in mulberry leaf. Moreover, no complete information is available about hunger signs of nutritional disorders, analytical diagnosis and critical levels of nutrients required. The present paper, thus, may serve as an important nutrient guide for identification of hunger signs, leaf nutrients status under deficiency and critical levels of the elements namely N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn requirements for higher leaf yield and quality. The leaf nutrient status provided may help chemist for correcting the soil status. Besides, an integration of mulberry intercropping with legumes and applications of neem and castor oil cakes, VA-mycorrhizal inoculation, biofertilizer and vermicompost are suggested as integrated nutrient management for sustainable sericulture industry. Based on the information described in this paper, a model needs to be framed for maintaining continuous supply of nutrients to obtain desired quantity and quality of mulberry leaf for successful silkworm cocoon crop and increasing overall silk productivity.

Leaf Blight of Chinese Chive Caused by Stemphylium vesicarium in Korea

  • Chang, Seog-Won;Kim, Hee-Dong;Kang, Hyo-Jung;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • 제16권5호
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    • pp.280-282
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    • 2000
  • A leaf spot disease occurred on Allium tuberosum Roth. (Chinese chive) in Korea. All the isolates of Stemphylium sp. from the lesions of the diseased plant parts were identified as S. vesicarium (Waller.) Simmons, based on the morphological characteristics of conidia and conidiophores. Pathogenicity of the fungus was proved by artificial inoculation on Chinese chive plants. This is the first record of leaf blight on Chinese chive caused by S. vesicarium in Korea.

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First Record of Alternaria simsimi Causing Leaf Spot on Sesame (Sesamum indicum L.) in Korea

  • Choi, Young Phil;Paul, Narayan Chandra;Lee, Hyang Burm;Yu, Seung Hun
    • Mycobiology
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    • 제42권4호
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    • pp.405-408
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    • 2014
  • Leaf spot disease was observed in sesame (Sesamum indicum L.) during 2009 and 2010 in Korea. The pathogen was identified as Alternaria simsimi based on morphological and cultural characteristics. The morphological identification was well supported by phylogenetic analysis of the ribosomal DNA-internal transcribed spacer region. A. simsimi isolates caused spot symptoms on leaves and stems of sesame plants 2 wk after artificial inoculation, which were similar to those observed in the field. This is the first record of leaf spot disease in Korea caused by A. simsimi.