Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Foliar Colonization and Growth Promotion of Red Pepper (Capsicum annuum L.) by Methylobacterium oryzae CBMB20

  • Lee, Min-Kyoung (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Chauhan, Puneet Singh (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Yim, Woo-Jong (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Lee, Gyeong-Ja (Chungbuk Agricultural Research and Extension Services) ;
  • Kim, Young-Sang (Chungbuk Agricultural Research and Extension Services) ;
  • Park, Kee-Woong (Bio-Evaluation Center, KRIBB) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2011.04.08
  • Accepted : 2011.05.13
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to exploit Methylobacterium oryzae CBMB20 as of plant growth promoting agent, different inoculation methods have been evaluated. The present study aimed to evaluate soil, foliar, and soil+foliar inoculations of M. oryzae CBMB20 to improve the growth, fruit yield, and nutrient uptake of red pepper (Capsicum annuum L.) under greenhouse conditions. The population range of green fluorescent protein (gfp)-tagged M. oryzae CBMB20 using the three inoculation methods was 2.5-2.9 ${\log}_{10}$ cfu/g in the rhizosphere and 4.5-6.0 ${\log}_{10}$ cfu/g in the phyllosphere of red pepper plants. Confocal laser scanning microscopy results confirmed the colonization of M. oryzae CBMB20 endophytically on leaf surface. Plant height, fruit dry weight, and total biomass were significantly higher ($p{\leq}0.05$) in all M. oryzae CBMB20 inoculation methods as compared to non-inoculated control. Furthermore, uptake of mineral nutrients such as N, P, K, Ca, and Mg in red pepper plants in all M. oryzae CBMB20 inoculation methods was higher than in non-inoculated control. Comparative results of inoculation methods clearly demonstrated that soil+foliar inoculation of M. oryzae CBMB20 lead to the highest biomass accumulation and nutrient uptake which may be due to its efficient colonization in the red pepper rhizosphere and phyllosphere.

Keywords

References

  1. Adesemoye AO, Torbert HA, and Kloepper JW (2008) Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Can J Microbiol 54, 876-886. https://doi.org/10.1139/W08-081
  2. Babalola OO (2010) Beneficial bacteria of agricultural importance. Biotechnol Lett 32, 1559-1570. https://doi.org/10.1007/s10529-010-0347-0
  3. Benediktyova Z and Nedbal L (2009) Imaging of multi-color fluorescence emission from leaf tissues. Photosynth Res 102, 169-175. https://doi.org/10.1007/s11120-009-9498-z
  4. Chauhan PS and Nautiyal CS (2010) The purB gene controls rhizosphere colonization by Pantoea agglomerans. Lett Appl Microbiol 50, 205-210. https://doi.org/10.1111/j.1472-765X.2009.02779.x
  5. Esitken, A, Pirlak L, Turan M, and Sahin F (2006) Effects of floral and foliar application of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrition of sweet cherry. Sci Horti 110, 324-327. https://doi.org/10.1016/j.scienta.2006.07.023
  6. Holland MA and Polacco JC (1994) PPFMs and other contaminants: Is there more to plant physiology than just plant? Annu Rev Plant Physiol Plant Mol Biol 45, 197-220. https://doi.org/10.1146/annurev.pp.45.060194.001213
  7. Jackson EF, Echlin HL, and Jackson CR (2006) Changes in the phyllosphere community of the resurrection fern, Polypodium polypodioides, associated with rainfall and wetting. FEMS Microbiol Ecol 58, 236-246 https://doi.org/10.1111/j.1574-6941.2006.00152.x
  8. Jackson ML (1973) In Soil Chemical Analysis. pp 54-56, Prentice-Hall of India Pvt. Ltd., New Delhi, India.
  9. Kim KA, Yim WJ, Trivedi P, Madhaiyan M, Deka Boruah HP, Islam MR, Lee GS, and Sa TM (2010) Synergistic effects of inoculating arbuscular mycorrhizal fungi and Methylobacterium oryzae strains on growth and nutrient uptake of red pepper (Capsicum annuum L). Plant Soil 327, 429-440. https://doi.org/10.1007/s11104-009-0072-4
  10. Kinkel LL (1997) Microbial population dynamics on leaves. Annu Rev Phytopathol 35, 327-347. https://doi.org/10.1146/annurev.phyto.35.1.327
  11. Madhaiyan M, Kim BY, Poonguzhali S, Kwon SW, Song MH, Ryu JH, Go SJ, Koo BS, and Sa TM (2007a) Methylobacterium oryzae sp. nov, a novel aerobic, pink-pigmented, facultatively methylotrophic, ACC deaminase producing bacterium isolated from field grown rice (Oryza sativa L. cv. Nam-Pyeoung). Int J Syst Evol Microbiol 57, 326-331. https://doi.org/10.1099/ijs.0.64603-0
  12. Madhaiyan M, Poonguzhali S, Kang BG, Lee YJ, Chung JB, and Sa TM (2010) Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice. Plant Soil 328, 71-82. https://doi.org/10.1007/s11104-009-0083-1
  13. Madhaiyan M, Poonguzhali S, Lee HS, Hari K, Sundaram SP, and Sa TM (2005) Pink-pigmented facultative methylotrophic bacteria accelerate germination, growth and yield of sugarcane clone Co86032 (Saccharum officinarum L). Biol Fertil Soils 41, 350-358. https://doi.org/10.1007/s00374-005-0838-7
  14. Madhaiyan M, Poonguzhali S, and Sa TM (2007b) Metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (Lycopersicon esculentum L). Chemosphere 69, 220-228. https://doi.org/10.1016/j.chemosphere.2007.04.017
  15. Omer ZS, Tombolini R, and Gerhardson B (2004) Plant colonization by pink-pigmented facultative methylotrophic bacteria (PPFMs). FEMS Microbiol Ecol 47, 319-326. https://doi.org/10.1016/S0168-6496(04)00003-0
  16. Pyrlak L and Kose M (2009) Effects of plant growth promoting rhizobacteria on yield and some fruit properties of strawberry. J Plant Nut 32, 1173-1184. https://doi.org/10.1080/01904160902943197
  17. Poonguzhali, S, Madhaiyan M, Yim WJ, Kim KA, and Sa TM (2008) Colonization pattern of plant root and leaf surfaces visualized by use of green-fluorescent-marked strain of Methylobacterium suomiense and its persistence in rhizosphere. Appl Microbiol Biotechnol 78, 1033-1043. https://doi.org/10.1007/s00253-008-1398-1
  18. Romanovskaia VA, Stoliar SM, Malashenko IR, and Dodatko TN (2001) The ways of plant colonization by Methylobacterium strains and properties of these bacteria. Microbiol 70, 221-227. https://doi.org/10.1023/A:1010441900060
  19. Sy A, Giraud E, Jourand P, Garcia N, Willems A, de Lajudie P, Prin Y, Neyra M, Gillis M, Boivin-Masson C, and Dreyfus B (2001) Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. J Bacteriol 183, 214-220. https://doi.org/10.1128/JB.183.1.214-220.2001
  20. Vijayan K, Chakraborti SP, and Ghosh PD (2007) Foliar application of Azotobactor chroococcum increases leaf yield under saline conditions in mulberry (Morus spp). Sci Horti 113, 307-311 https://doi.org/10.1016/j.scienta.2007.03.018
  21. Vogelmann TR and Evans CJ (2002) Profiles of light absorption and chlorophyll within spinach leaves from chlorophyll fluorescence. Plant Cell Environ 25, 1313-1323. https://doi.org/10.1046/j.1365-3040.2002.00910.x

Cited by

  1. conditions to promote plant growth vol.116, pp.2, 2013, https://doi.org/10.1111/jam.12384
  2. Effects of growth-promoting endophytic Methylobacterium on development of Citrus rootstocks vol.10, pp.19, 2016, https://doi.org/10.5897/AJMR2016.7926
  3. Methylobacterium oryzae CBMB20 influences photosynthetic traits, volatile emission and ethylene metabolism in Oryza sativa genotypes grown in salt stress conditions vol.249, pp.6, 2011, https://doi.org/10.1007/s00425-019-03139-w