• Title/Summary/Keyword: leaf and stem

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Peroxidase Isozyme Pattern and Polyamine Contnts in Germinating Peas (Pisum staivum) (완두 발아시 Polyamine 함량 변화 및 Peroxidase Isozyme 양상)

  • 표병식
    • Journal of Plant Biology
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    • v.36 no.4
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    • pp.307-312
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    • 1993
  • In germinating pea, contents of enodgenous polyamine in the leaf and stem were determined, and protein content, peroxidase activity and pattern of isozymes were examined in the leaf treated with polyamines. During growth of the pea for 14 days in light condition, the polyamines in leaf and stem showed the highest level at the 5th day, and were decreased rapidly at the 7th day, kept almost constant level since then. The putrescine level was relatively higher than those of spermidine and spermine, and cadaverine was also detected. On the other hand, in the leaf treated with spermine (0.01 mM) protein content increased about 250% than that of the control, the peroxidase activity increased ore than 100% in spermine of 0.01 mM and 0.1 mM. In treating with putrescine of 0.1 mM the pattern of peroxidase isozyme appeared 4 new cathodic bands (pI 4.8, 5.6, 5.9 and 6.8) compared with the control, the clear cathodic bands (pI 5.6, 5.9, 6.4 and 6.6) were also observed in spermine of 0.1 mM. These results suggest that polyamines were important factor in the differentiation of pea at the early stage of germination.

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Interrelationship of Biological Yield and Harvest Index in Mulberry and Its Association with Shoot Rearing in Silkworm (Bombyx mori L.)

  • Susheelamma, B.N.;Kamble, Chandrakanth;Dandin, S.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.2
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    • pp.143-147
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    • 2002
  • Studies on the interrelationship between biological yield and harvest index, and their association with shoot rearing were carried out in 9 promising mulberry genotypes, which indicated significant variations among genotypes. Biological yield had close association with leaf yield components indicating that they are inter-related for higher productivity in mulberry, Biological yield revealed highly significant positive correlation with important leaf yield components. However height of the longest branch showed negative correlation with biological yield. Genotypes with higher biological yield associated with low ratio of stem weight to leaf weight and high Harvest index on dry weight basis are to be selected for shoot rearing. Biological yield recorded on fresh weight basis had less impact on the present study in comparison to dry weight basis.

Studies on Dry Matter Production and Variation of Agronomic Characteristics of Determinate and Indeterminate Types of Soybean Cultivars (Glycine max L.) Under Different Growing Condition (유ㆍ무한형대두품종의 재배조건에 따른 건물생산 및 형질변이에 관한 연구)

  • Keun-Yong Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.45-78
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    • 1974
  • To provide useful information for developing new high yielding soybean varieties and for improving cultural practices, an investigation was made on variation of dry matter production and on relationship among several agronomic characters of soybean plants grown under different planting times and densities as well as under different fertilizer levels, using Kwang-kyo, Dong puk-tae, and Suke # 51 as determinate types and Shelby, SRF-300 and Harosoy as indeterminate types at the Crop Experiment Station during the period of 1972 and 1973. The results obtained were summarized as follow: 1. The dry weight, CGR and LAI at the initial flowering stage were high in the high plant population irrespective of varieties, planting times, and fertilizer levels. However, those characters of the indeterminate type were lower than those of the determinate types. The same characters of the indererminate type at the terminal leaf stage were either same or higher than those of the determinate types. 2. The dry weight of the determinate type at the initial flowering stage was similar to the indeterminate, type, when planting times were May 21 or June 15. The dry weights of both types of varieties were low when planted on July 10. When fertilizer levels were increased, the CGR, dry weight and LAI at the initial flowering stages were also increased. 3. Even though significant differences of LAI were obtained among the varieties within the same plant type, the indeterminate type was in general lower than that of the determinate type regardless of planting time and densities, or fertilizer levels, while the yield of the indeterminate type was comparable to the yield of the determinate type. 4. The high degree of leaf- and petiole-fall at the greenbean stage was highly associated with early planting and high levels of fertilizers. However, less amount of leaf- or petiole-fall was found when planted on July 10 or under low plant population. 5. The percent of stem weight was high under higher plant population, while the percent of leaf weight was high under lower plant population. When planting time was late, the percent of stem and petiole weight were reduced, while the leaf weight was increased. 6. The percent of pod weight of the determinate type at the terminal leaf stage was about 2% when planted on May 21, about 8% when planted on June 15, and about 9% when planted on July 10. The percent of pod weight of the indeterminate type at the terminal leaf stage were about 6 % when planted on May 21, 14% when planted on June 15 and 21% when planted on July 10. 7. Kwang kyo showed less degree of leaf-fall even when lodged due to high levels of fertilizer applied, while SRF-300 showed great damage due to lodging. 8. High yields were obtained when planted on May 21, but there were little yield differences between yields from May 21 and June 15 plantings. The reduction of yield due to late planting of July 10 was less apparent in the determinate type of varieties, while it was high in the indeterminate type. 9. The optimum plant population per are for high yield was 1, 250 to 2, 500 plants when planted on May 21, 2, 500 plants when planted on June 15, and 3, 333 plants when planted on July 10. 10. High correlation coefficients were obtained between dry matter weight and LAI at the terminal leaf stages, and between the dry matter weight and yield at the greenbean stages. The optimum dry weight for high yield in the determinate type was expected to be 25 kg. per are at the initial flowering stage and 50 kg. per are at the terminal leaf stage. In the indeterminate type the LAI and dry weight at the greenbean stage were 4 to 5 and 80 kg. per are, respectively. 11. Under the high plant population plant height was increased, while the stem diameter and the number of nodes and branches were reduced. Consequently, the percent of mainstem to main stem plus branches were increased, and the length of internode was also elongated. The ratios of stem weight, number of nodes and pods, and yield of main stem were increased when high plant population was associated with the early planting. The percent of main stem to branches for the indeterminate type was higher than that of the determinate type. 12. Under the high plant densities and late planting, the percent of the pod number and yields of main stem were increased, indicating that varieties with no or less branches were better adaptable under such conditions. 13. High degree of simple correlation coefficients was obtained between the LAI at the initial flowering stage and terminal leaf stage, and the total node number, dry matter and dry stem weight of both determinate and indeterminate types. Even though no significant correlation was found between the LAI at the initial flowering stage of the determinate type and the stem length and pod number per are, highly significant correlation coefficients were obtained between such characters in the indeterminate type of varieties. 14. The dry matter was positively correlated with the LAI, CGR, stem length, and pod number, node number and dry stem weight per are, while no significant correlation was found between the dry matter and stem diameter. 15. The correlation coefficients between lodging index and the LAI, dry weight, stem length and dry stem weight were highly significant. Negative correlation was obtained for the indeterminate type between the stem diameter and lodging index. The correlation coefficient between the stem diameter and lodging index was non-significant for the determinate type, while positive correlation was obtained between the yield and lodging index in the determinate type. The lodging index was also positively correlated with average length of internode of main stem. 16. The 100 seed weight appeared to be lowered under the high plant population and no fertilizer condition, and when planted late. Apparent differences of 100 seed weight were found between main stem and branches, being higher for the main stem than for the branches. 17. No variation of protein content was found due to different cultural practices. However, the oil content was apparently reduced when planted late.

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Physiological disorder of Panax ginseng (인삼의 생리장해)

  • 박훈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.5
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    • pp.459-480
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    • 1991
  • Physilogical disorders of P. ginseng occurred in farmer's field were reviewed in relation to symptom. In root, red skin, rough skin, rust, root rot complex, round root, fine root stripe, freezing injury, cracking, sleeping and uneven emergence were frequently appeared. In leaf and stem, yellowing, early defoliation, leaf bum, Papery leaf, white freezing injury, wind injury, stem cracking were the main troubles. Red skin of root and leaf yellowing gave the greatest negative impact on ginseng production. Some cases of damage by pesticides, excess boron and industrial pollutants were reported. Physiological disorders related to quality factors, such as inside cavity, inside white sponge-like ect. after processing were discussed.

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Effects of Spray Times and Ventilation Method on the Seedling Growth of Fruit Vegetables (관수회수 및 송풍처리가 과채류의 묘 생장에 미치는 영향)

  • Kim Chang-Soo;Min Byeong-Ro;Kim Wong;Kim Dong-Woo;Seo Kwang-Wook;Lee Beom-Seon;Lee Dae-Weon
    • Journal of Bio-Environment Control
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    • v.14 no.1
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    • pp.1-6
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    • 2005
  • A multipurpose operating system was developed to adjust both spray times and ventilation method without a configuration of the moving path and the type of the greenhouse. The multipurpose working system proved to be a reliable system for testing the growth quality of the fruit vegetables in the greenhouse. The results are as follows. The first leaf, diameter of a stem, leaf area, and average stem diameter in the Cucumber seedling growth were repressed by high-speed ventilation, but was not repressed by spray times. The first leaf in the Tomato seedling growth was repressed as ventilation velocity was high, but the average stem diameter was not repressed. While the Tomato was given water three times a day, the diameter of a stem and the leaf area were increased as ventilation speed became higher. However, those were different other factors. The Tomato leaf area was larger when given water twice a day than that in hand spray, but showed no difference with ventilation speed. The first leaf, the diameter of a stem and the leaf area of a Red pepper were lower in automatic spraying with ventilation than those in hand spray.

Calibration Update for the Measuring Total Nitrogen Content in Rice Plant Tissue Using the Near Infrared Spectroscopy

  • Kwon, Young-Rip;Song, Young-Eun;Choi, Dong-Chil;Ryu, Jeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.1
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    • pp.29-35
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    • 2009
  • The aim of the present study was to update the calibration that is used for the measurement of the total nitrogen content in the rice plant samples by using the visible and near infrared spectrum. Before the equation merge, correlation coefficient of calibration equation for nitrogen content on each rice parts was 0.945 (Leaf), 0.928 (Stem), and 0.864 (Whole plant), respectively. In the calibration models created by each part in the rice plant under the various regression method, the calibration model for the leaf was recorded with relatively high accuracy. Among of those, the calibration equation developed by Partial least squares (PLS) method was more accurate than the Multiple linear regression (MLR) method. The calibration equation was sensitive based on variety and location variations. However, we have merged and enlarged various of the samples that made not only to measure the nitrogen content more accurately, but also later sampling populations became more diversified. After merging, $R^2$ value becomes more accurate and significantly to 0.950 (L.), 0.974 (S.), 0.940 (W.). Also, after removal of outlier, R2 values increased into 0.998, 0.995, and 0.997. In view of the results so far achieved, Standard error of prediction (SEP) and SEP (C) were reduced in the stem and whole plant. Biases were reduced in the leaf, stem as well as whole plant. Slopes were high in the stem. Standard deviation reduced in the stem but $R^2$ was high in the stem and whole plant. Result was indicated that calibration equation make update, and updating robust calibration equation from merge function and multi-variate calibration.

Antioxidant Effects of Ethanol Extracts from Different Parts of Portulacae Herba (마치현 부위별 에탄올 추출물의 항산화 활성)

  • Jeong, Kyung-Hee;Ji, Yoon-Sun;Kil, Ki-Jung;Yoo, Ji-Hyun
    • The Korea Journal of Herbology
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    • v.34 no.1
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    • pp.59-65
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    • 2019
  • Objectives : This study reviewed the antioxidant activity of each part of Portulacae Herba (PH) in order to better analyze the possibility of PH being used as a natural material. Methods : The root, stem, and leaves of the PH were separated, dried at $50^{\circ}C$, put in a 70% ethanol and then extracted three times every six hours. Antioxidant activities was performed by measuring the total polyphenol and total flavonoid contents, DPPH, ABTS, nitrite, hydroxyl radical scavenging activity, and $Fe^{2+}$ chelating. Results : The total polyphenol and total flavonoid contents was highest in the root, then the leaves, and lastly the stem. DPPH and ABTS radical scavenging activities for the root extract indicated the highest activity followed by the leaf and stem extracts. It also showed similar activity to that of the ascorbic acid. Hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity resulted to be highest in the root extract and then the leaf and stem extracts. Nitrite scavenging activity decreased with higher pH levels, and activity was highest in the order of root extract, leaf extract, and stem extract. Also, in reaction to a solution with a pH 1.2 level, the root extract showed similar scavenging activity to that of BHT. Conclusions : Therefore, based on the above results, it was verified that the roots of PH have a high antioxidant level, and they can be considered to have potential to be applied to functional food, cosmetics, and medicine with antioxidant efficacy.

In vitro cytotoxic activity of ginseng leaf/stem extracts obtained by subcritical water extraction

  • Lee, Kyoung Ah;Kim, Kee-Tae;Chang, Pahn-Shik;Paik, Hyun-Dong
    • Journal of Ginseng Research
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    • v.38 no.4
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    • pp.289-292
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    • 2014
  • Ginseng leaf/stem extract produced by subcritical water extraction at high temperature ($190^{\circ}C$) posses higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

Effects of Cadmium on Growth of Arabidopsis thaliana (카드뮴이 애기장대의 생장에 미치는 영향)

  • Park Jong-Bum
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1103-1108
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    • 2004
  • This experiment was carried out to investigate the effects of cadmium on the growth of Arabidopsis thaliana when they were treated with different concentrations of cadmium. The growth of stem was stimulated in the concentrations up to fifty times higher than the official standard concentration of cadmium of pollutant exhaust notified by the Ministry of Environment, but it decreased in the concentration one hundred fifty times or more higher in proportion to the degree of concentration. The growth of root was similar to that of stem, except that the decrease was gradual in the concentration fifty times or more higher. The growth of leaf was almost the same as that of stem, that is, it was stimulated the increase of leaf surface area in the concentration fifty times higher, but decreased in the concentration one hundred fifty times or more higher in proportion to the degree of concentration. The fresh weights of the plants were increased in accord with the degree of growth of the stem and leaf. Concentration of cadmium accumulated in the plants was increased in proportion to the concentration of cadmium. These results show that the growth of plants was stimulated in the soil polluted by cadmium up to fifty times higher than the official standard concentration, but it was decreased in proportion to the degree of concentration in the plants grown in the presence of cadmium more than one hundred fifty times.