• Title/Summary/Keyword: latex agglutination test

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Serological survey of diseases to poultry and swine in slaughtered ostriches (도축 타조에서 닭 및 돼지 질병에 대한 혈청학적 조사)

  • Kim Soon-Tae;Park In-Hwa;Kim Young-Hoan;Cho Kwang-Hyun;Oh Kyu-Shil;Son Jae-Kweon;Jyeong Jong-Sik
    • Korean Journal of Veterinary Service
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    • v.27 no.3
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    • pp.281-288
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    • 2004
  • As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

Seroepidemiological study of Toxoplasma gondii infection in the rural area Okcheon-gun, Korea

  • Lee, Young-Ha;Hoh, Hyung-Jun;Hwang, Ok-Sun;Lee, Sang-Keol;Shin, Dae-Whan
    • Parasites, Hosts and Diseases
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    • v.38 no.4
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    • pp.251-256
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    • 2000
  • There have been some reports about the prevalence of anti-Toxoplasma gondii antibody among Koreans, and most of all data were taken from patients visiting hospitals. However, the epidemiological data of the community-based study in Korea are rare. This study was performed to evaluate the seroprevalence of toxoplasmosis among the inhabitants of the rural area Okcheon-gun, Korea. A total of 1,109 serum samples (499 males, 610 females) were examined for the IgG antibodies by ELISA. To set up the cut-off point for ELISA, we used a commercial latex agglutination (LA) kit. The sensitivity and specificity of ELISA against LA test were 89.5%, and 98.6% respectively. Among 1,109 sera, 6.9% showed seropositivity by ELISA. The positive rates of males and females were 6.0% and 7.2%, respectively. However, there were no significant differences between sexes. Comparing the age groups, the highest seropositive rate showed in the seventies or higher, and their rates had a tendency to increase with age (0.05 < p < 0.3). These results revealed that the seroprevalence of toxoplasmosis in rural inhabitants is similar to previous reports in Korea; however we need further investigation to clarify the prevalence of toxoplasmosis in the general population.

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Investigation of stray cats Toxoplasmosis in Seoul area (서울지역 길고양이의 Toxoplasma 감염증 실태조사)

  • Kim, Neung-Hee;Chae, Hee-Sun;Han, Hye-Jin;Son, Hong-Rak;Kim, Chang-Ki;Kim, Sun-Heung;Lee, Jung-Hark;Kim, Chul-Hun
    • Korean Journal of Veterinary Service
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    • v.32 no.3
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    • pp.275-279
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    • 2009
  • Toxoplasma gondii is one of the most common protozoa parasites of human and other warm-blooded animals. Cats and wild felidae play crucial roles in the epidemiology of toxoplasmosis. This study was performed to survey the prevalence of T. gondii infection among stray cats in the Seoul. A total of 422 stray cat blood samples were collected from Seoul area. Positive sera for T. gondii were identified in 56 samples (13.3%) exclusively via the latex agglutination test and the detection no antigen particles among seropositive samples by PCR. The overall infection rate of male stray cats (14.4%) presented as higher than that of female cats (10.7%). This study suggested that T. gondii is widespread in stray cats of Seoul area. It is needed to control urban stray cat population and to reduced the risk of zoonotic transmission of toxolasmosis to other animals and humans.

Toxoplasma gondii antibody titers in sera of children admitted to the Seoul National University Children's Hospital

  • Kook, Jina;Lee, Hong-Jin;Kim, Beyong-Il;Yun, Chong-Ku;Guk, Sang-Mee;Seo, Min;Park, Yun-Kyu;Hong, Sung-Tae;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.37 no.1
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    • pp.27-32
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    • 1999
  • A total of 542 children under 10 years of age, admitted to the Seoul National University Children's Hospital, was examined for antibody titers of Toxoplasma gondii using indirect latex agglutination (ILA) test. Among them, 7.7% showed positive titers higher than 1:32, without significant difference between males (7.3%) and females (8.5%). The seropositive rate increased with age although the statistical significance was negligible (0.05

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Toxoplasma Antibodies by Indirect Latex Agglutination Tests in Zoo Animals (동물원 동물에서의 Latex응집반응에 의한 Toxoplasmu항체가)

  • 최원영;유재을
    • Parasites, Hosts and Diseases
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    • v.25 no.1
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    • pp.13-23
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    • 1987
  • Total of 216 animals conserved in Seoul Grand Park were examined on the antibody titers of Toxoplasma by the indirect latex agglutination test, 20 out of 131 cases (15.3%) in mammals, 2 out of 75 cases (2.7%) in birds, and none in reptiles, according to species, 15 out of 68 species (22.1%) in mammals, 2 out of 36 species (5.6 %) in birds showed positive antibody titers when the titers of 1 : 32 or higher were regarded as positive. In mammals, it appeared as positive in 2 out of 6 cases (1 out of 3 species) in marsupials, 1 out of 15 cases (1 out of 11 species) in primates, lout of 1 case in bats, 6 out of 13 cases (5 out of 10 species) in carnivores, 1 in 12 cases (1 species out of 3) in odd-toed ungulates, 9 out of 80 cases (6 species out of 38) in even-toed ungulates, and none in rodents and in whales. In birds, 1 out of 21 cases (1 out of 7 species) in gallinaceous birds and lout of 6(5 species) in parrots appeared to have the positive antibody titers of Toxoplasma. And, none of reptiles showed positive. Frequencies of positive antibody titers were high in 1 : 64, 9 cases in mammals, followed by 1 : 32, 6 cases, 1 : 128, 3 cases, and 1 : 256, 2 cases, respectively. Two positive cases in birds appeared to be 1 : 64.

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The Occurrence and Molecular Characterization of Feline Cryptococcosis in Korea

  • Kim, Yoon Hee;Song, Ae-ra;Hwang, Soo Myung;Hong, Yeon-Jung;Choi, Ul Soo
    • Journal of Veterinary Clinics
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    • v.35 no.4
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    • pp.170-173
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    • 2018
  • A spayed female domestic short-hair cat of unknown age was admitted with a large proliferative mass in the face. Cytology and biopsy results suggested infection with Cryptococcus spp. A latex cryptococcal antigen agglutination test and an ALPHA cryptococcal antigen enzyme immunoassay yielded positive results. Results of canavanine-glycine-bromothymol blue agar test, serotyping and molecular typing by URA5 - RFLP and MLST analysis identified the isolates as C. neoformans var. grubii VNI/ST31. Two other cats were also diagnosed with the same methodology showing Crytococcosis with VNI/ST31. Cats presenting with facial or respiratory signs should be assessed for cryptococcosis in Korea.

Pseudomembranous Colitis in a Child of Chronic Diarrhea (만성 설사 환아에서의 위막성 대장염 1례)

  • Lee, Jin;Kim, Jong-Wan;Kim, Seung-Il
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.1 no.1
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    • pp.138-143
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    • 1998
  • Chronic diarrhea in children is a common problem with numerous causes. Although most of these causes are benign, critical illness may present as chronic diarrhea. In a patient of chronic diarrhea, gastrointestinal infections are the most common causes in children of all ages and antibiotics may cause chronic diarrhea by altering intestinal microflora, which can result in the emergence of bacterial overgrowth. Overgrowth of Clostridium difficile may cause pseudomembranous colitis. We experienced 25-month-old boy who suffered from chronic diarrhea and partially treated with antibiotics irregularly. Colonoscopic findings of this child showed multiple plaques with white to yellowish exudate which adhere to the mucosal surface of a variable length of rectum. Histollogically, each plaque comprised a pseudomembrane of mucous debris, inflammatory cells, and exudate overlying groups of partially disrupted glands. A latex agglutination test on patient's stool was positive to toxin A of Clostridium difficile. He was recovered after stopping the antibiotics he has been prescribed, and being given vancomycin for 2 weeks. We report this case with brief review of literature.

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A case of Cryptococcal Meningitis (크립토코크스 뇌막염 1례 보고)

  • O, Khyoung-Yhun;Byun, Young-Ju;Park, Choong-Suh;Jeon, Chang-Ho;Kim, Chung-Sook
    • Journal of Yeungnam Medical Science
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    • v.4 no.1
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    • pp.139-143
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    • 1987
  • The clinical picture and CSF findings in cryptococcus meningitis may be identical with those of tuberculous meningitis. The differential diagnosis can be made by finding the budding yeast organism in the counting chamber or in stained smear, the detection of cryptococcal antigen in CSF by the latex agglutination test, and by culture of the fungus on Sabouraud agar. We experienced a case of cryptococcal meningitis in the 48 years old woman, which was confirmed by Indian ink preparation and culture.

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Prevalence of pili and enterotoxins of Escherichia coli associated with diarrhea in preweaning piglets (포유자돈 소장에서 분리된 대장균의 섬모항원과 장내독소 분포양상)

  • Ham, Hee-jin;Cheon, Doo-sung;Chae, Chan-hee
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.779-784
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    • 1997
  • A comprehensive study of 132 Escherichia coli isolates from 150 piglets with colibacillosis included detection of heat-labile enterotoxin, heat-stable enterotoxin, and identification of K88 (F4), K99 (F5), 987P (F6), and F41. Four pili were examined by haemagglutination and slide agglutination test. Heat-labile(LT) and heat-stable(ST) enterotoxin was determined by reverse passive latex agglutination and precipitation test, respectively. Among 132 E coli isolates, 26 had K88 (19.7%), 16 had K99 (12.1%), 3 had 987P (2.3%), and 2 had F41 (1.5%). Three had K88 and K99 (2.3%), 3 had K88 and 987P (2.3%), 2 had K99 and 987P (1.5%), 5 had K99 and F41 (3.8%), and 8 E coli strains had K88, K99 and F41 (6.1%) simultaneously. Among 132 E coli isolates, 5 produced LT only (3.8%), 55 produced heat-stable toxin ST only (41.7%), and 4 produced both LT and ST (3.0%). Three major pathotypes accounted for 27.9% of E coli isolates: $K99^+$ (8.3%), $K88^+ST^+$ (9%) and $K88^+$ (10.6%). Results of this study indicated that piliated enterotoxin-producing E coli was prevalent and was associated with diarrhea in preweaning piglets.

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Studios on Infection of Haemophilus Pleuropneumoniae in Slaughtered Pigs (도축돈에서의 Haemophilus Pleuropneumoniae 감염에 관한 연구)

  • Lee Hak-Cheul;Yoo Byong-Sam
    • Journal of Veterinary Clinics
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    • v.3 no.1
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    • pp.243-253
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    • 1986
  • In recent years much attention has been paid to swine respiratory infection caused by Haemophilus(H.) Pleuropneumoniae with rapid expansion of pork industry in Korea. The organism may cause an acute respiratory infection with high morbidity and mortality, or it may also cause chronic persistent infection, resulting in loss of body weight. The present study was performed to observe the etiologic situation of H. Pleuropneumoniae infection by evaluating bacteriological, serological examinations with the lungs and sera collected from slaughtered pigs in Daegu city, respectively. In addition, antibiotic susceptibility test was carried out against the isolated strains of H. Pleuropneumoniae. The results obtained are summarized as follows : 1. Only 10 strains of H. Pleuropneumoniae were isolated from the 120 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were identical with that of standard strains(serovar 1 to 6). 2. Out of 336 swine sera examined, 108(32.1%) were shown to be positive against H. Pleuropneumoniae by latex agglutination test. 3. Antibiotic susceptibility test revealed that 10 isolates of H. Pleuropneumoniae were sensitive to 16 kinds of antibiotic such as ampicillin, benzylpenicillin, piperacillin, cephalothin, cefazolin, cefamandol, cefoperagon, lactomoxycefa, tetracycline, minocycline, chloramphenicol, gentamicin, kanamycin, amikacin, colistin and nalidixic acid, whereas cloxacillin, erythromycin, kitasamycin, lincomycin, oleand-amycin, and bacitracin were resistent.

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