• BMB Reports
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• v.42 no.3
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• pp.136-141
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• 2009

Familial Amyotrophic lateral sclerosis (FALS) is a progressive neurodegenetative disorder induced by mutations of the SOD1 gene. Heat shock protein 27 (HSP27) is well-defined as a stress-inducible protein, however the its role in ALS protection has not yet been established. To investigate the role HSP27 may have in SOD1 mutant-mediated apoptosis, human SOD1 or HSP27 genes were fused with a PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame fusion protein, which was then transduced into cells. We found the purified PEP-1-HSP27 fusion proteins can be transduced efficiently into neuronal cells and protect against cell death by enhancing mutant SOD1 activity. Moreover, transduced PEP-1-HSP27 efficiently prevents protein aggregation produced by oxidative stress. These results suggest that transduced HSP27 fusion protein may be explored as a potential therapeutic agent for FALS patients.

• Journal of Wetlands Research
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• v.14 no.4
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• pp.439-455
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• 2012

Coupled wave induced current modeling system(WIC) was developed from combining with the nearshore spectral wave model, SWAN, the wave induced force model, WIF, and the flow model, EFDC. The reasonable results were obtained from WIC modeling system. The ratio of the wave height calculated with respect to refraction and diffraction effects over submerged spherical shoal was occurred approximately 1~5 % errors compared to Goda(2000)'s result. The radiation stress suggested by Longuet-Higgins and Stewart(1960), the stresses due to rollers in breaking waves proposed by Dally and Osiecki(1994), and Kim(2004)'s new spreading approach instead of the previous lateral mixing approach were added to calculate wave induced force. The results of the WIC modeling system show good agreement with Nishimura et al.(1985)'s laboratory measurements and better than Kim(2004)'s 2 dimensional depth averaged numerical computations for a plane beach with detached breakwater. The present flow field computed agrees reasonably well with the measured flow field. The relative merit of WIF model in WIC modeling system is unconditional stable for time increment.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.5
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• pp.1-10
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• 2012

This paper presents an experimental result and suggests the confinement effect of headed cross tie in reinforced concrete(RC) columns subjected to cycling horizontal loads under constant axial load. Five RC columns specimens were manufactured, taking confined type of transverse reinforcement, whether or not using cross tie, end detail of cross tie (hooked or headed), and axial stress in column as major variables, Cyclic horizontal load applied to the columns under constant axial stress and the effect of cross tie to structural capacity of column was evaluated from the test. The column without cross tie failed showing bending deformation of hoop with crack in core concrete at low horizontal load while the column with cross tie showed quite improved strength and ductility by suppressing bending deformation of hoop as well as buckling of longitudinal bar at once even after crack in core concrete. At high lateral displacement, the column with hooked cross tie showed the failure pattern loosing the confining force of cross tie since the $90^{\circ}$ hooked part of cross tie was stretched out and the cracked core concrete lumps were came off. However, the column with headed cross tie showed very stable behavior since the head of cross tie effectively confined the hoop and longitudinal bars even at high lateral displacement.

• Journal of Korean Society of Steel Construction
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• v.30 no.1
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• pp.1-12
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• 2018

A curved member should resist bending and torsional moments simultaneously even though the primary load is usually supposed to be gravitational load. The torsional moment causes complicate stress state and also can result in early yielding of material to reduce member strength. According to analysis results, the strength of a curved member that has 45 degrees of subtended angle could decrease more than 50% compare to straight girder. Nevertheless, there have been very few of researches related with ultimate strength of curved girders. In this study, various kinds of stiffness about bending, pure torsion and warping were considered with a number of models in order to verify the main factor that affects ultimate behavior of curved girder. Lateral and rotational displacement of curved member were introduced as lateral-torsional-vertical behavior and bending-torsional moment interaction curve was derived. Finally, a strength equation for ultimate moment of horizontally curved steel I-girders subjected to equal end moments based on the interaction curves. The equation could take account of the effect of curvature, unbraced length and sectional properties.

• Journal of the Korean Wood Science and Technology
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• v.44 no.1
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• pp.30-39
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• 2016

The evaluation of the lateral load performance for larch glulam portal frames was carried out using glass fiber reinforced plastic (GFRP) as connector in two different systems: the GFRP-reinforced laminated plates combined with veneer, and GFRP rod joints glued with epoxy resins to replace usual metal connectors for the structural glulam rahmen joints. As a result the yield strength, ultimate strength, initial stiffness of glulams of GFRP rod joints glued with epoxy resin decreased to 49%, 52% and 61% compared to those of the conventional metal connector. This connector will be a stress device where the bonding strength between the GFRP rod and glued laminated timber is important. Thus, there will be a high possibility that a problem may occur when it is applied to the field. On the other hand, the GFRP-reinforced laminated plates and wood (Eucalyptus marginata) pin were measured all within 3% for all measurements of the yield strength, ultimate strength, initial strength and ductility factor, regardless of high cross sectional loss on the glued laminated timber slit joint. In addition, the variation of stiffness on the cycle was 35%, which was the lowest, confirming that it was almost the same performance as the specimen prepared with the metal connector.

• Journal of the Korean Geotechnical Society
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• v.30 no.10
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• pp.33-44
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• 2014

The preliminary design of suction pile as the supporting system for concrete floating structures was performed for the pilot project of the southwest coast area in Korea. Prior to starting design work, site conditions of the area including ground and hydraulic conditions, and a 100-year return period external force were throughly evaluated. The suction pile for mooring of the offshore floating structures has to satisfy the lateral resistance against external force as well as the penetration ability according to the soil conditions such as soil types, shear strengths, effective stresses, and seepage forces. In the design, the required penetration depths, which were stable for lateral resistance, were evaluated with the diameters of cylindrical suction pile as the final installing ones. And the design suction pressures at each penetrating depths, at which sand boiling did not occur, were assessed through the comparison of penetration and penetrationresistance forces. As a result, it was impossible for suction piles with the diameter range of 3.0~5.0 m to penetrate into required penetration depths. On the other hand, suction piles with the diameter range of 6.0 m and 7.0 m satisfied both the horizontal stability and the penetration ability by design suction pressures at the required penetration depths of 8.5 m and 8.0 m, respectively.

• Molecules and Cells
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• v.25 no.1
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• pp.55-63
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• 2008

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder characterized by the selective death of motor neurons. Mutations in the SOD1 gene are responsible for a familial form of ALS (FALS). Although many studies suggest that mutant SOD1 proteins are cytotoxic, the mechanism is not fully understood. To investigate the role of mutant SOD1 in FALS, human SOD1 genes were fused with a PEP-1 peptide in a bacterial expression vector to produce in-frame PEP-1-SOD fusion proteins (wild type and mutants). The expressed and purified PEP-1-SOD fusion proteins were efficiently transduced into neuronal cells. Neurones harboring the A4V, G93A, G85R, and D90A mutants of PEP-1-SOD were more vulnerable to oxidative stress induced by paraquat than those harboring wild-type proteins. Moreover, neurones harboring the mutant SOD proteins had lower heat shock protein (Hsp) expression levels than those harboring wild-type SOD. The effects of the transduced SOD1 fusion proteins may provide an explanation for the association of SOD1 with FALS, and Hsps could be candidate agents for the treatment of ALS.

• Journal of Korean Ophthalmic Optics Society
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• v.17 no.2
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• pp.171-176
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• 2012

Purpose: This study was to compare changes in near lateral phoria and near point of convergence after viewing smartphones and monitors during short periods. Methods: 50 subjects with healthy eyes from 20s to 30s (mean age, $21.84{\pm}5.13$ years) were examined. Viewing time was set at 5 min to 20 min, and recovery time was 10 min. Near lateral phoria and near point of convergence (NPC) were examined before and after watching smartphones and monitors, and subjectively symptoms for visual fatigue were surveyed using a questionnaire before and after viewing image. Results: The tendency of phoria in viewing smartpones found more exophoric results than in viewing monitors, and both exophoric shifts were recovered after 10 min. The receded NPC in the smartphones was more remote than in the monitors. The smartpones induced more visual fatigue than the monitors. Conclusions: Sustained smartphone works induced changes in phoria and NPC, and this type of stress could cause visual fatigue. Therefore, an appropriate rest breaks can be helpful in relieving visual fatigue.

• Journal of the Korea Concrete Institute
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• v.18 no.5 s.95
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• pp.595-602
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• 2006

An experimental study was conducted to investigate seismic behaviour of post-tensioned(PT) exterior slab-column connections used for the purpose to resist gravity loads only. For these, 2/3-scale, two PT post-tensioned exterior connections with two different tendon arrangement patterns and one conventional reinforced concrete(RC) exterior connection was tested under quasi-static, uni-directional reversed cyclic loading. During the lateral testing, gravity forces transferred to the column were kept constant to closely simulate a moment to shear ratio of a real building. One of the objectives of this study was to assess the necessity and/or the quantity of bottom bonded reinforcement needed to resist moment reversal which would occur under significant inelastic deformations of the adjacent lateral force resisting systems. The ACI 318 and 352 provisions for structural integrity were applied to provide the bottom reinforcement passing through the column for the specimens. Prior test results were also collected to conduct comparative studies for some design parameters such as the tendon arrangement pattern, the effect of post-tensioning forces and the use of bottom bonded reinforcement. Consequently, the impact of tendon arrangement on the seismic performance of the PT connection, that is lateral drift capacity and ductility, dissipated energy and failure mechanism, was considerable. Moreover, test results showed that the amount of bottom reinforcement specified by ACI 352. 1R-89 was sufficient for resisting positive moments arising from moment reversal under reversed cyclic loads. Shear strength of the tested specimens was more accurately predicted by the shear strength equation(ACI 318) considering the average compressive stress over the concrete($f_{pc}$) due to post-tensioning forces than that without considering $f_{pc}$.

• The korean journal of orthodontics
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• v.31 no.1 s.84
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• pp.25-38
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• 2001

The purpose of this study was to compare the force, the displacement and the stress distribution on the maxillary first molars altered by the application of various asymmetric head-gear. For this study, the finite element models of unilateral Cl II maxillary dental arch was made. Also, the finite element models of asymmetric face-bow was made. Three types of asymmetric face-bow were made : each of the right side 15mm, 25mm and 35mm shorter than the left side. We compared the forces, the displacement and the distribution of stress that were generated by application of various asymmetric head-gear, The results were as follows. 1. The total forces that both maxillary first molars received were similar in all groups. But the forces that mesially positioned tooth received were increased as the length of the outer-bow shortened, and the forces that normally positioned tooth received were decreased as the length of the outer-bow shortened. 2. In lateral force comparison, the buccal forces that normally positioned tooth received were increased as the length of the outer-bow shortened, and the buccal fortes that mesially positioned tooth received were decreased as the length of the outer-bow shortened. Though the net lateral force moved to the buccal side of normally positioned tooth as the length of the outer-bow shortened, both maxillary first molars received the buccal force. That showed 'Avchiai Expansion Effect' 3. The distal forces, the extrusion forces and the magnitudes of the crown distal tipping that mesially positioned tooth received were increased as the length of the outer-bow shortened, and the forces that normally positioned tooth received were decreased as the length of the outer-bow was shortened. 4. The magnitude of the distal-in rotation that normally positioned tooth received were increased as the length of the outer-bow was shortened. But, mesially positioned tooth show two different results. For the outer-bow 15mm shortened, mesially positioned tooth showed the distal-in rotation, hut for the outer-bow 25mm and 35mn shortened, mesially positioned tooth showed the distal-out rotation. Thus, the turning point exists between 15mm and 25mm. 5. This study of the initial stress distribution of the periodontal ligament at slightly inferior of the furcation area revealed that the compressive stress in the distobuccal root of the normally positioned tooth moved from the palatal side to the distal side and the buccal side successively as the length of the outer-bow shortened. 6. This study of the initial stress distribution of the periodontal ligament at slightly inferior of the furcation area revealed that the magnitudes of stress were altered but the total stress distributions were not altered in the mesiobuccal root and the palatal root of normally positioned tooth, and also three roots of mesially positioned tooth as the length of the outer-bow shortened.