• Title/Summary/Keyword: lateral root development

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Establishment of Seed Treatment for Healthy Production of Peanut Sprout (청정 땅콩나물 생산을 위한 종자처리기술 확립)

  • Park, Eun-Ji;Lee, Gyu-Bin;Heo, You;Son, Beung-Gu;Choi, Young-Whan;Lee, Yong-Jae;Park, Young-Hoon;Suh, Jeong-Min;Kang, Jum-Soon
    • Journal of Environmental Science International
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    • v.24 no.6
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    • pp.755-762
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    • 2015
  • The present study was conducted to develop seed treatment for the production of healthy and clean peanut sprout. Dry heat treatment of peanut seeds reduced the incidence of the rot. The seed treatment condition at $52^{\circ}C$ for 10 h. was the most efficient without inhibiting seed viability significantly. Seeds were dark cultured at $27^{\circ}C$ for up to 9 days. The treatment of Indole-B and gibberellic acid influenced germination, T50, fresh, dry weight, hypocotyl length, hypocotyl length diameter, root length, number of lateral root and epicotyl of peanut sprout. There were no differences in the germinability of peanut seeds between gibberellic acid treatment methods but higher fresh weight was observed in the GA3 solution spray after 2 hour water soaking. The general growth and lateral root development of peanut sprouts were suppressed by Indole-B which is used for inhibiting root formation and promoting hypocotyls. The treatment of gibberellic acid promoted hypocotyl elongation, but it did not influence on the growth of hypocotyls and root system.

Root metabolic cost analysis for root plasticity expression under mild drought stress

  • Kano-Nakata, Mana;Mitsuya, Shiro;Inukai, Yoshiaki;Yamauchi, Akira
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.328-328
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    • 2017
  • Drought is a major limiting factor that reduces rice production and occurs often especially under recent climate change. Plants have the ability to alter their developmental morphology in response to changing environment, which is known as phenotypic plasticity. In our previous studies, we found that one chromosome segment substitution line (CSSL50 derived from Nipponbare and Kasalath crosses) showed no differences in shoot and root growth as compared with the recurrent genotype, Nipponbare under non-stress condition but showed greater growth responses compared with Nipponbare under mild drought stress condition. We hypothesized that reducing root respiration as metabolic cost, which may be largely a consequence of aerenchyma formation would be one of the key mechanisms for root plasticity expression. This study aimed to evaluate the root respiration and aerenchyma formation under various soil moisture conditions among genotypes with different root plasticity. CSSL50 together with Nipponbare and Kasalath were grown under waterlogged conditions (Control) and mild drought stress conditions (20% of soil moisture content) in a plastic pot ($11cm{\times}14cm$, ${\varphi}{\times}H$) and PVC tube ($3cm{\times}30cm$, ${\varphi}{\times}H$). Root respiration rate was measured with infrared gas analyzer (IRGA, GMP343, Vaisala, Finland) with a closed static chamber system. There was no significant difference between genotypes in control for shoot and root growth as well as root respiration rate. In contrast, all the genotypes increased their root respiration rates in response to mild drought stress. However, CSSL50 showed lower root respiration rate than Nipponbare, which was associated by higher root aerenchyma formation that was estimated based on internal gas space (porosity) under mild drought stress conditions. Furthermore, there were significant negative correlations between root length and root respiration rate. These results imply that reducing the metabolic cost (= root respiration rate) is a key mechanism for root plasticity expression, which CSSL50 showed under mild drought.

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Utilizing chromosome segment substitution lines (CSSLs) to evaluate developmental plasticity of root systems in hardpan penetration and deep rooting triggered by soil moisture fluctuations in rice

  • Nguyen, Thi Ngoc Dinh;Suralta, Roel R.;Mana, Kano-Nakata;Mitsuya, Shiro;Stella, Owusu Nketia;Kabuki, Takuya;Yamauchi, Akira
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.321-321
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    • 2017
  • Water availability in rainfed lowlands (RFL) is strongly affected by climate change. In RFL, rice plants are exposed to soil moisture fluctuations (SMF) but rarely to simple progressive drought as widely believed. Typical RFL field is characterized by a about 5-cm thick high bulk density hardpan layer underneath the cultivated layer at about 20 cm depth that impedes deep root development. Root system has the ability to develop in response to changes in SMF, known as phenotypic plasticity. We hypothesized that genotypes that can adapt to RFL have root plasticity. The roots can sharply respond to re-wetting after drought period and thus penetrate the hardpan layer when the hardpan is wet and so becomes relatively soft, and thus access water under the hardpan. This study aimed to identify CSSLs derived from a cross between Sasanishiki and Habataki which adapted to such RFL conditions. We used 39 CSSLs together with the parent Sasanishiki, which were grown in hydroponics and pot under transient soil moisture stresses (drought and then rewatering), and compared with continuously well-watered (WW) (control) up to 14 days after sowing (DAS), and 20 DAS, respectively. Based on the results of hydroponics and pot experiments, we selected a few lines, which were grown in the soil-filled rootbox with artificial hardpan layer and without artificial hardpan. For the rootbox without artificial hardpan, plants were grown under WW and transient soil moisture stresses for 49 DAS. While the rootbox with artificial hardpan, the plants were grown under WW (control) and SMF (WW up to 21 DAS, 1st drought (22-36 DAS), rewatering (37-44 DAS), and followed by 2nd drought (45-58 DAS)). Among the 39 CSSLs, only CSSL439 (SL39) consistently showed significantly higher shoot dry weight (SDW) than Sasanishiki under transient soil moisture stress conditions as well as SMF conditions in all the experiments. Furthermore, under WW, SL39 consistently showed no significant differences from Sasanishiki in shoot and root growth in most of traits examined. SL39 showed significantly greater total root length (TRL) than Sasanishiki under transient soil moisture stress, which is considered as phenotypic plasticity in response to rewatering after drought period. Such plastic root development was the key trait that effectively contributed to root elongation and branching during the rewatering period and consequently enhanced the root to penetrate hardpan layer when the soil penetration resistance at hardpan layer reduced. In addition, using the rootbox with artificial hardpan layer ($1.7g\;cm^{-3}$, heavily compacted), SL39 showed greater root system development than Sasanishiki under SMF, which was expressed in its significantly higher TRL, total nodal RL, and total lateral RL at hardpan layer as well as at below the hardpan layer. These results prove that SL39 has plasticity that enables its root systems to penetrate hardpan layer in response to rewatering. Under SMF, such root plasticity contributed to its higher gs and Pn.

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Study on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas 11. On the Histological Differences Between the Roots of the Salt, Land and Water Bed Seedlings of Rice and their Rooting in Saline Soil (간척지에서 수도 및 기타작물의 내염성에 관한 연구 11. 수도의 염분묘 및 수묘근의 조직학적 차이와 염분지에서의 발근에 관하여)

  • 임형빈
    • Journal of Plant Biology
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    • v.13 no.4
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    • pp.13-22
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    • 1970
  • The rice variety, Kwanok, was reared in the water, land and salt seed beds and transplanted to the reclaimed soil area having an average of 0.48% salt content (0.67% at the end of April). The plant height of land bel seedlings at transplanting stage was short but the dry-weight/plant-height ratio was large and the rooting ability was vigorous remarkably after transplantation in the salty area. The central cylinder, vessels, sclerenchyma, endodermis and other mechanical tissues of the root of land bed seedlings were well developed while the size of cortical cell layers were small. The cytoplasm of the cortical parenchyma at the root tips seemed to be most abundant in the land bed seedlings. The formation of the aerial cavity in the cortex of primary root was rapid and it seemed that the developmental mechanism of the aerial cavity in the rice plant roots was related to the development of the lateral roots.

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In vitro Micropropagation of Rosa hybrid L.

  • Kim Chang-Kil;Oh Jung-Youl;Jee Sun-Ok;Chung Jae-Dong
    • Journal of Plant Biotechnology
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    • v.5 no.2
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    • pp.115-119
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    • 2003
  • To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

PULP RESPONSES TO AN ELECTRIC PULP STIMULATOR IN THE DEVELOPING PERMANENT ANTERIOR DENTITION (영구전치(永久前齒)의 치근발육단계(齒根發育段階)에 따른 Electric Pulp Stimulator에 대(對)한 치수반응(齒髓反應))

  • Choi, Jae-Hong
    • Journal of the korean academy of Pediatric Dentistry
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    • v.6 no.1
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    • pp.27-33
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    • 1979
  • The author studied the response to the stimulation of an electric pulp stimulator of healthy 854 permanent anterior teeth in 122 children aged from six to eleven years old, during different stages of tooth development. The results were as followings: 1) In completely open apices, 13.1% (18 teeth) showed positive responses, and 86.9% (120 teeth) showed negative responses. In two-thirds open apices 33.1% (75 teeth) showed positive responses, and 66.9% (152 teeth) showed negative responses. In one-third open apices 57.8% (118 teeth) showed positive responses, and 42.2% (86 teeth) showed negative responses. In closed apices 80.0% (228 teeth) showed positive responses, and 20.0% (57 teeth) showed negative responses. 2) The number of positive responses increased in each upper and lower central incisors during the stages of root development, but not in upper lateral incisors. 3) There were no significant differences statistically in responses between the teeth of the right and left sides and the upper and the lower jaw, in the same stages of root development.

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RELATIONSHIP BETWEEN THE DEVELOPMENTAL STAGE AND CHRONOLOGICAL AGE, AND THE CHANGES OF TOOTH POSITION IN RELATION TO THE TOOTH DEVELOPMENT ON MANDIBULAR PERMANENT TEETH (하악 영구치아의 발육과 연령과의 관계 및 치아 발육에 따른 치아의 위치 변화)

  • Kim, Hyun-Mi;Yang, Seung-Duck;Kim, Hyun-Jung;Kim, Young-Jin;Nam, Soon-Hyeun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.4
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    • pp.607-617
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    • 2002
  • The purposes of this study were to evaluate the timing of tooth calcification and the change of tooth position with tooth developmental stage on the mandibular teeth. Seven hundred seventy two children(male:446, female:326), 3 to 12 years of age were examined radiographically with panoramic film. Dental development was determined by inspecting radiographs and assigning a rating according to consecutive stages defined by Moorrees, and tooth cusp position and root terminus position were measured from the lower border of mandibular body and calculated the position index to evaluate the movement of tooth with developmental stage. The results were as follows. 1. There were no significant differences between boys and girls in the timing of calcification until crown completion, but timing of calcification tend to be faster in girls than in boys after root initiation stage. 2. In terms of mean age, crown completion of central incisor in boys and girls occurred at the age of 3.71, 4.05 years, at 4.44, 4.60 years for the lateral incisor, at 5.35, 5.11 years for the canine, at 6.62, 6.36 years for the first premolar, at 7.36, 7.17 years of second premolar, at 3.51, 3.69 years of first molar, and at 7.90, 7.64 years for the second molar respectively. Apex 1/2 closed stage of central incisor occurred at the age of 8.70 in boys, 8.18 in girls, at 9.55, 8.99 years for the lateral incisor, at 12.48, 11.60 years for the canine, at 12.30, 12.01 years for the first premolar, at 12.19, 12.26 years of second premolar, at 9.12, 8.87 years of first molar, and at 12.59, 12.45 years for the second molar respectively. 3. There was no noticeable movement of cusp tip until crown completion (Crc), but showed rapid movement toward occlusion plane after root initiation(Ri) and again maintain stable position after root completion stage(Rc). 4. Root terminus position was stable until root 1/4 formation stage(R1/4), followed by rapid movement toward occlusal plane and was stable again after root 3/4 formation stage(R3/4). 5. Developmental stage at the time of alveolar bone penetration by cusp tip varied with each of the permanent teeth. 6. Canine tooth follicle was at the lowest position in the mandibular body during the early stage of calcification, followed by second premolar, first premolar, lateral incisor, second molar, first molar and central incisor in order.

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A lateral approach to the maxillary sinus for simultaneous extraction of an ankylosed maxillary molar and sinus graft: a case report

  • Hwang, Jae-Ho;Choi, Hee-Seung;Kim, Kee-Deog;Doh, Re-Mee;Park, Won-Se
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.38 no.2
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    • pp.110-115
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    • 2012
  • Ankylosed tooth is defined as 'the discontinuance of normal passive tooth eruption without any mechanical barrier'. Ankylosed tooth treatment is a challenge to dental clinicians. In treatment of maxillary molar ankylosis cases there are risks of oro-antral fistula, displacement of root fragments into the maxillary sinus, as well as the necessity for providing additional sinus bone augmentation for future implant placement. In this study, we suggested a new technique using a piezoelectric device and a lateral side approach to the maxillary sinus leading to the simultaneous removal of the ankylosed maxillary molar and sinus grafting for the purpose of implant site development.

Growth, yield and mineral element responses to temperature in Codonopsis lanceolata

  • Kwon, Soo Jeong;Han, Eun Ji;Moon, Young Ja;Cho, Gab Yeon;Boo3, Hee Ock;Lee, Moon Soon;Woo, Sun Hee;Kim, Hag Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.274-274
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    • 2017
  • Temperature has an adverse effect for potential growth and development of Codonopsis lanceolata. The present study examined the responses of growth, yield and mineral element contents under temperature variables in C. lanceolata. Plant height exhibited the best result as being 2 m or higher the open field culture, while the growth is the most restricted in the $30^{\circ}C$. The open field culture also had the largest number of leaves in the early stage of growth. The number of leaves and the height tended to be increased with the temperature of $20^{\circ}C$ and $25^{\circ}C$. The number of branches showed no significance as 32 branches and 26 branches in open field culture and $20^{\circ}C$, respectively. The smallest number of branches was observed in the $30^{\circ}C$ with 15 branches. The leaf color recorded the lowest value in the open field culture unlike the growth above the ground. But all other temperature had no significance with the leaf color value between 50.0 and 52.6. The elevated temperature induced the thinner and shorter diameter and length of root. However, the root diameter was not significantly changed with the change of 1.8 cm and 2.1 cm at open field culture, with $20^{\circ}C$ and $25^{\circ}C$, respectively. The fresh weights of the primary root and lateral roots were 6.3 g and 3.1 g, respectively, at the high temperature. The result showed the thickening of the lowest part under the ground as restricted. The fresh weights of the main root and lateral root were the highest on the open field culture, 20.6 g and 12.2 g, respectively. The contents of cellulose were the highest as 4.41% in the $20^{\circ}C$, followed by the open field culture, $25^{\circ}C$ and $30^{\circ}C$. The higher temperature reduced the contents of cellulose. Moreover, P showed higher ratio in the high temperature while Ca and Mg exhibited the higher ratio towards the low temperature. On the contrary, minor quantity (less than 1%) of mineral nutrients was observed towards all temperature variables.

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Numerical Study on Ultimate Lateral Resistance of Roots of Vegetation (식물뿌리의 극한 횡방향 저항력에 관한 수치해석적 연구)

  • Lee, Seung-Hyun;Kwon, Oh-Soon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.9
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    • pp.4205-4209
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    • 2011
  • Numerical analysis were executed in order to investigate ultimate lateral resistance of roots of vegetation. Ultimate lateral resistances of roots obtained from the assumed values of cohesions were distributed between the values of the two kinds of the existing studies. The ultimate lateral resistance values were more close to those by the bearing capacity solution than those by the cavity expansion theory. Coefficient of bearing capacity determined by the numerical analysis was 33. Yielding displacements obtained from the numerical analysis were 0.08~0.29 times of the diameter of the root and those were overall close to the value of the existing study which was undertaken for the pile diameter of 1 cm.