Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

In vitro Micropropagation of Rosa hybrid L.

  • Kim Chang-Kil (Department of Horticulture, Sangju National Univ.) ;
  • Oh Jung-Youl (Department of Horticulture, Sangju National Univ.) ;
  • Jee Sun-Ok (Division of Life Sciences, Joongbu University) ;
  • Chung Jae-Dong (Department of Horticulture, Kyungpook National Univ.)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

Keywords

References

  1. J Am Soc Hortic Sci v.107 Factors affecting in vitro propagation of rose Bressan PH;Kim Y;Hyndman SE
  2. Plant Cell Tissue Organ Cult v.21 Organogenesis and plant regeneration for immature embryos of Rosa hybrida L. Burger DW;Liu L;Zary KW https://doi.org/10.1007/BF00033434
  3. Plant Cell Tissue Organ Cult v.32 Effect of liquid culture on the growth and development of miniature rose (R. chinensis Jacq. ‘Minima’) Chu CY;Knight SI;Smith MAL https://doi.org/10.1007/BF00042296
  4. Scientia Hortic v.13 Rapid propagation of roses in vitro Davies DR
  5. Acta Hortic v.289 Micropropagation in roses Dohare SR;Shafi M;Kaicker US
  6. J Am Soc Hortic Sci v.114 Comparison of hydathode structure in micropropagated plantlets and greenhouse-grown ‘Queen Elizabeth’ rose plants Donelly DJ;Skelton FE
  7. Acta Hortic v.298 In vitro preservation at low temperature of rose plantlets usable for direct acclimatization Dorion N;Kadri M;Bigot C
  8. Plant Cell Tissue organ Cult v.19 Micropropagation of floribunda, ground cover and miniature roses Douglas GC;Rutledge CB;Casey AD https://doi.org/10.1007/BF00037776
  9. HortSci v.17 Stimulation of root initiation from culured rose shoots through the use of reduced concentrations of mineral salts Hyndman S;Hasegawa PM;Bressan RA
  10. Plant Cell Tissue Organ Cult v.1 The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots Hyndman S;Hasegawa PM;Bressan RA https://doi.org/10.1007/BF02318919
  11. Agroplantae v.2 Tissue culture studies on rose: Use of shoot tip explants. II. Cytokinin : gibberellin effects Jacobs G;Allan P;Bornman CH
  12. Agroplantae v.2 Tissue culture studies on rose: use of shoot tip explants. III. Auxin: gibberellin dffects Jacobs G;Allan P;Bornman CH
  13. HortSci v.29 Performance of micropropagated ‘Queen Elizabeth’ rose following mechanically induced stress Korban M;Donnelly DJ
  14. The complete book of roses Krussmann G
  15. Acta Hortic v.227 Influence of sucrose concentration of the photosynthetic ability of in vitro grown rose shoots Langford PJ;Wainwright H
  16. Euphytica v.37 The induction in vitro of adventitious shoots in Rosa Lloyd D;Roberts AV;Short KC https://doi.org/10.1007/BF00037220
  17. Plant Cell Tissue Organ Cult v.24 A protoplast to plant system in roses Metthews D;Mottley J;Horan I https://doi.org/10.1007/BF00033473
  18. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco cultures Murashige T;Skoog F https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  19. Proc Eucarpia Symp. Integration of in vitro techniques in ornamental plant breeding Wageningen Protoplast technology and somatic embryogenesis in Rosa Roberts AV;Horan I;Matthews D;de Jong J(ed.)
  20. Acta Hortic v.319 The effects of growth retardants, humidity and lighting at stage III on stage IV of micropropagation in chrysanthemum and rose Roberts AV;Walker S;Horan I
  21. J Hortic Sci v.667 Consequences of in vitro and vitro root initiation for minature rose production Rogers RB;Smith MAL
  22. Plant Cell Tissue Organ Cult v.27 Somatic embryogenesis in callus cultures of Rosa hybrida L. cv. Landora Rout GR;Debata BK;Das P https://doi.org/10.1007/BF00048208
  23. Biotechnology in agriculture and forestry. Vol. 15. Medicinal and aromatic plants III v.15 Rosa spp. (Roses); In vitro culture, micropropagation, and the production of secondary products Short KC;Roberts AV;Bajaj YPS(ed.)
  24. Handbook of plant cell culture Rose Skirvin RM;Chu MC;Young HJ;Ammirato PV(ed.);Evans DR(ed.);Sharp WR(ed.)
  25. Sci Hortic v.15 Anther culture of Rosa Tabaeezadeh A;Khosh-Kui M https://doi.org/10.1016/0304-4238(81)90062-5