• Parasites, Hosts and Diseases
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• 제58권3호
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• pp.287-299
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• 2020

Cystic echinococcosis (CE) is a zoonotic infection caused by Echinococcus granulosus larvae. It seriously affects the development of animal husbandry and endangers human health. Due to a poor understanding of the cystic fluid formation pathway, there is currently a lack of innovative methods for the prevention and treatment of CE. In this study, the protoscoleces (PSCs) in the encystation process were analyzed by high-throughput RNA sequencing. A total of 32,401 transcripts and 14,903 cDNAs revealed numbers of new genes and transcripts, stage-specific genes, and differently expressed genes. Genes encoding proteins involved in signaling pathways, such as putative G-protein coupled receptor, tyrosine kinases, and serine/threonine protein kinase, were predominantly up-regulated during the encystation process. Antioxidant enzymes included cytochrome c oxidase, thioredoxin glutathione, and glutathione peroxidase were a high expression level. Intriguingly, KEGG enrichment suggested that differentially up-regulated genes involved in the vasopressin-regulated water reabsorption metabolic pathway may play important roles in the transport of proteins, carbohydrates, and other substances. These results provide valuable information on the mechanism of cystic fluid production during the encystation process, and provide a basis for further studies on the molecular mechanisms of growth and development of PSCs.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권1호
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• pp.11-17
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• 2005

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

• 한국수산과학회지
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• 제34권4호
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• pp.378-383
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• 2001

실험에 사용한 성숙한 난소를 가진 산모양깔깔새우 (Metapenaeopsis dalei)는 1999년 8월 11일 충남 외연도 근해에서 새우조망을 이용하여 채집하였다. 산란한 유생을 4개의 염분구간과 3개의 수온구간 및 5개의 다른 먹이 조건에서 사육하여 이들의 생존율과 성장률을 비교하였다. 염분조건에서는 채집지의 염분과 유사한 30과 35에서 생존율이 높았고, 수온 조건에서는 산란 수온$24^{\circ}C$에서 생존율이 가장 높았다. 먹이 조건 실험에서는 식물성먹이에서 생존율과 성장이 동물성을 투여한 실험구보다 높았다.

• Fisheries and Aquatic Sciences
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• 제8권1호
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• pp.27-33
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• 2005

This is a subsequent study to our previous finding that Pacific oysters, Crassostrea gigas, gained a so-called upper plateau concentration, around 30,000 ng/g dry weight digestive gland for benzo(a)pyrene, showed reproductive behavior but produced their ensuing reproductive outputs damaged. A serial dilution of sediment elutriates from Jinhae Bay, Korea, where pollution was progressive, were exposed to gametes of the Pacific oyster for 0, 5, 10, 20, 30, and 60 min to detail the pollutant effects on very young specimens. There was an apparent critical dilution over which adverse effects are evident. This was $10\%$ of the present sediment elutriate, corresponding to 0.3 ng/g on the basis of total polycyclic aromatic hydrocarbons (PAHs) for the oyster. Within the dilution the embryonic development was not influenced by the duration of exposure to its gamete stage. At higher dilutions over the critical dilution, occurrence of abnormality increased dependent on the pollutant dilution and the duration of exposure. Similar trends were also found in larval mortality. However, overall, the chemical toxicity was more significant to morphogenesis than to survival, suggesting a potential recruitment of the pollutants-induced abnormal larvae in the wild population to threaten the population integrity.

• 한국패류학회지
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• 제27권3호
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• pp.175-180
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• 2011

본 연구는 말백합의 안정적인 인공종묘생산을 위해 먹이생물의 종류 및 공급량을 조사하였고, 일정기간 절식 후 먹이생물의 재공급에 따른 영향을 관찰하였다. 유생의 먹이생물은 I. galbana, C. calcitrans, N. oculata를 혼합하거나 단독으로 공급할 경우 C. calcitrans를 D형 유생 1개체당 3,000-5,000 세포를 공급하는 것이 가장 효과적이었다. D형 유생을 일정기간 절식 후 먹이생물을 공급한 경우 먹이생물을 공급한 시점부터 4일째에 평균각장 $192.5{\mu}m$ 이상의 침착기 유생으로 성장하였다. 생존율은 절식기간이 길수록 낮아지는 경향을 보였다.

• 한국응용곤충학회지
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• 제29권4호
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• pp.269-276
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• 1990

전남 지방의 단감나무에 기생하는 짝지벌레 중 우점종인 뿔밀깎지벌레의 기주범위 발생소장 및 각 태별 생육특성을 조사한 결과는 다음과 같다. 기주식물은 총 34과 66종이 조사 되었으며 그 중 나팔꽃, 명아주, 콩, 망초, 들깨, 사철베고니아등 6종이 새로이 조사되었다. 각 태별 발생소장은 난이 5월 상순-6월 하순까지 발생하였으며 최성기는 6월 상순이었고 유충은 6월 중순-10 월 중순까지 존재하고 최성기는 7월 상순-8월 중순이었으나 화학적 방제가 가장 효과적인 시기는 6월 하순으로 추정된다. 산란수는 평균 1,073 $\pm$ 177.3개 이었고 부화율은 평균 97.3%로 광주기 및 처리온도에는 크게 반응하지 않았다. 각 태별 기간은 란이 23.4일, 유충이 128.3일, 자성충은 213.3일이었다.

• IMMUNE NETWORK
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• 제2권4호
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• pp.195-201
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• 2002

Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-2H3 has been well documented in the rat mucosal mast cell line. Methods: RBL-2H3 cells sensitized with each kind of rat IgE was activated by cross-linking FcRI with B5 (monoclonal anti-rat IgE mouse IgG antibodies). The RBL-2H3 exocytosis was measured by analyzing the ${\beta}$-hexosaminidase level, and the level of IL-4 mRNA synthesis was analyzed using semiquantitative RT-PCR. Rat IgE, which was produced by a parasite infection (REP), was prepared using either Paragonimus westermani metacercariae (REP-PW) or Anisakis simplex third stage larvae (REP-AS). A rat IgE prototype of IR162 was prepared by a peritoneal injection of immunocytoma. Results: The level of exocytosis showed a linear relationship with the rat IgE concentration when REP-PW or REP-AS was applied. However, it exhibited a biphasic response with IR162. In addition, the time course of heating at $56^{\circ}C$ illustrated the similarity between REP-PW and REP-AS, which differed from that of IR162. In contrast, the level of IL-4 mRNA synthesis in the RBL-2H3 cells with IR162 was comparable to that of either REP-PW or REP-AS. Conclusion: These results suggest that functionally different rat IgE isoforms exists in RBL-2H3 exocytosis.

• 한국어류학회지
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• 제24권2호
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• pp.94-100
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• 2012

우리나라 고유종인 기름종개의 난발생 및 초기생활사를 연구하기 위해 2007년 6월에 전라북도 남원시 운봉읍의 낙동강에서 성숙한 개체를 채집하였다. 채집된 개체는 LHRH-1을 주사하여 건식법으로 인공수정시켰다. 성숙란은 분리침성란으로 밝은 회색이었으며, 난경은 $1.29{\pm}0.07mm$였다. 수정난은 수온 $23{\sim}25^{\circ}C$에서 수정 후 45~52시간 사이에 부화하였으며 크기는 전장 $4.5{\pm}0.24mm$였다. 부화 후 5일 후에 난황이 모두 흡수 되어 후기자어로 이행하고 전장 $6.6{\pm}0.13mm$로 성장하였으며, 부화 후 25일에는 지느러미 기조가 모두 정수로 되어 치어기로 이행하였고 전장 $9.0{\pm}0.49mm$로 성장하였다. 부화 후 100일 후에 반문모양과 외부형태가 성어와 유사하였고 전장 $27.3{\pm}2.82mm$로 성장하였다.

• Animal cells and systems
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• 제6권1호
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• pp.75-80
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• 2002

A study on the effects of bisphenol A (BPA) on sex differentiation and gonadal development in medaka, Oryzias latipes, was investigated by histological examination. The fish were exposed to aqueous solutions of BPA at nominal concentrations of 50, 100, and 200 $\mu\textrm{g}$/L from newly-hatched larvae stage to 70 d. The ovaries of female fish were composed of oocytes at the chromatin nucleolus and peri-nucleolus stages at 20 d after the exposure. The testes contained a number of spermatogonia and spermatocytes at 30 d. In the process of sex differentiation. gonadal development was not different in all experimental groups until 30 d after the exposure. At 70 d after the exposure, however, advanced development of oocytes in the ovary and inhibition of spermatogenesis in the testis were observed in the BPA-treated groups compared to the non-treated controls. More females than males were identified in the 50 and 100 $\mu\textrm{g}$/L BPA-treated groups, in comparison to the 200 $\mu\textrm{g}$/L BPA-treated group and non-treated controls. Medaka exposed to 200 $\mu\textrm{g}$/L BPA were bigger compared to other experimental groups. The present study suggests that BPA may lead to problems in either mating or sexual behavior due to the difference in growth and disparity of sexual maturation between male and female fish.

• 한국응용곤충학회지
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• 제34권3호
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• pp.229-233
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• 1995

우리나라 주요 녹차재배지에 발생하는 동백가는나방(차굴나방 전가칭. Caloptilia theivora (Walshingham))의 생활사와 발생소장을 조사한 결과는 다음과 같다. 동백가는나방은 야외 사육상 조건에서 년 6세대 경과하였는데, 난에서 우화까지의 평균기간은 봄.가을에는 32.1~38.6일, 여름에는 24.8~32.7일이었으며, 성충수명은 봄.가을에 8.4~14.5일, 여름에는 6.3~8.6일이었고 산란수는 55~71개였다. 유충의 연중 발생소장을 보면, 1992~1993년에는 5월 중순에 발생 peak를 보이기 시작하여 3~4회의 peak가 나타났으나 1994년에는 발생량이 감소하여 초기에는 뚜렷한 발생 peak가 나타나지 않다가 9월 하순~10월 초순에 발생량이 가장 많았으며, 잎에서 용태로 월동하였다. 동백가는나방의 유증기생봉으로는 Stenomesius japonicus (Ashmead). Sympiesis ringoniellae Kamijo, S. dolichogaster Ashmead와 Elasmus sp.등 4종이 채집 동정되었으며, S. dolichogaster가 우점종이다.