• Title/Summary/Keyword: large source

Search Result 2,545, Processing Time 0.033 seconds

Production of Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid by Pseudomonas sp. HJ (Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산)

  • 손홍주;민관필이상준
    • KSBB Journal
    • /
    • v.10 no.4
    • /
    • pp.349-356
    • /
    • 1995
  • To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were $37^{\circ}C$ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

  • PDF

Evaluation of Angle Dependence on Positional Radioisotope Source Detector using Monte Carlo Simulation in NDT (몬테카를로 시뮬레이션을 이용한 방사선원 위치 검출기의 각도의존성 연구)

  • Han, Moojae;Heo, Seunguk;Shin, Yohan;Jung, Jaehoon;Kim, Kyotae;Heo, Yeji;Lee, Deukhee;Cho, Heunglae;Park, Sungkwang
    • Journal of the Korean Society of Radiology
    • /
    • v.13 no.1
    • /
    • pp.141-146
    • /
    • 2019
  • Radiation sources used in the field of industrial non-destructive pose a risk of exposure due to ageing equipment and operator carelessness. Thus, the need for a safety management system to trace the location of the source is being added. In this study, Monte Carlo Simulation was performed to analyse the angle dependence of the unit-cell comprising the line-array dosimeter for tracking the location of radiation sources. As a result, the margin of error for the top 10% of each slope was 5.90% at $0^{\circ}$, 8.08% at $30^{\circ}$, and 20.90% at $60^{\circ}$. The ratio of the total absorbed dose was 83.77% at $30^{\circ}$ and 53.36% at $60^{\circ}$ based on $0^{\circ}$(100%) and showed a tendency to decrease with increasing slope. For all gradients, the maximum number was shown at $30^{\circ}$ No. 9 pixels, and for No. 10, there was a tendency to drop 7.24 percent. This study has shown a large amount of angle dependence, and it is estimated that the proper distance between the source and line-array dosimeters should be maintained at a distance of not less than 1 cm to reduce them.

A Study on the Storytelling and its Application : Focusing on the Panmunjom and the surrounding DMZ (판문점 및 주변 DMZ 지역의 스토리텔링과 활용방안에 관한 연구)

  • Lee, Dongmi
    • 지역과문화
    • /
    • v.6 no.4
    • /
    • pp.23-45
    • /
    • 2019
  • The main research is on how to find source stories and develop and utilize content that will properly show the value of Panmunjom and the surrounding DMZ on the Korean Peninsula, which are receiving worldwide attention. Three methods were used as research for the high-end branding of Panmunjom and the surrounding DMZ. The first is the interest of prospective visitors through the travel cycle, using Joseph Campbell's "The Hero's Journey." The second was to incorporate Roland Bart's "third meaning" into the subjective travel and emotional stimulation, which is the trend of modern travel. The third introduced Kevin Lynch's theory and tried to place five elements of the path, edge, district, node and landmark in the core location of Panmunjom. Through the study, the excavation of contents in Panmunjom and the surrounding DMZ needs to be collected to a third meaning using a direct interview method that listens to the stories of related figures as well as organizing historical events. The priority should be given to those over the age of 80 and interviews should be conducted at least three times and over three generations. The process of building it into an archive, selecting a story, and then going through a culture collage and networking and branding takes place in five stages. This can create a virtuous circle of content processing, content utilization, job creation in the region and revitalizing the local economy through discovery of source stories and storytelling. Content development should be able to touch sensibility while saving time, history, place, originality and sincerity. Taking the center between tourism and travel, development and damage concerns, a careful but long-term plan and large-scale discovery of source stories should be made, and a consensus should be formed on the need for proper storytelling.

Dynamics of RNA Bacteriophage MS2 Observed with a Long-Lifetime Metal-Ligand Complex

  • Kang, Jung Sook;Yoon, Ji Hye
    • Journal of Photoscience
    • /
    • v.11 no.1
    • /
    • pp.35-40
    • /
    • 2004
  • [Ru(2,2'-bipyridine)$_2$(4,4'-dicarboxy-2,2'-bipyridine)]$^{2+}$(RuBDc) is a very photostable probe that possesses favorable photophysical properties including long lifetime, high quantum yield, large Stokes' shift, and highly polarized emission. To evaluate the usefulness of this luminophore (RuBDc) for studying macromolecular dynamics, its intensity and anisotropy decays when conjugated to RNA bacteriophage MS2 were examined using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. The intensity decays were best fit by a sum of two exponentials, and the mean intensity decay time was 442.2 ns. The anisotropy decay data showed a single rotational correlation time (2334.9 ns), which is typical for a spherical molecule. The use of RuBDc enabled us to measure the rotational correlation time up to several microseconds. These results indicate that RuBDc can be useful for studying rotational diffusion of biological macromolecules.s.

  • PDF

Roles of Glucose and Acetate as Carbon Sources in L-Histidine Production with Brevibacterium flavum FERM1564 Revealed by Metabolic Flux Analysis

  • Shioya, Suteaki;Shimizu, Hiroshi;Shimizu, Nobuyuki
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.7 no.3
    • /
    • pp.171-177
    • /
    • 2002
  • The metabolic flux pattern for L-histidine production was analyzed when glucose and/or acetate were used as carbon sources. Total L-histidine production was enhanced when mixed substrate (glucose and acetate) was used, compared wish that when either glucose or acetate was used as the sole carbon source. Theoretical maximum carbon fluxes through the main pathways for L-histldine production, cell growth, and ATP consumption for cell maintenance were obtained by the linear programming (LP) method. By comparison of the theoretical maximum carbon fluxes tilth actual ones, it was found that a large amount of glucose was actually used for maintenance of cell viability. On the other hand, acetate was used for cell growth. After depletion of acetate in the mixed substrate culture, the flux for glucose to L-histldine synthesis was markedly enhanced. A strategy for effective L-histidine production using both carbon sources was proposed.

A Study on The Fat Measurement at Subcutaneous Adipose by Optical and Electrical Method (광전 방식에 의한 피하 지방층의 비만도 측정에 관한 연구)

  • Oh, Se-Yong;Lee, Young-Woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2008.05a
    • /
    • pp.405-407
    • /
    • 2008
  • Body fat measures a large number places body because error is oversized that measure in single specification region to measure body whole body fat degree by non-invasive optical method and bio-electrical impedance method. Use LED source of light that center wavelength is 660nm wavelength and measure at same time by BIA(Bio-electrical Impedance Analysis) method And then photo-electricity method calculate fat correlation formula.

  • PDF

Voltage Sag Compensation using dq Stationary and Synchronous Rotating Frame (dq 정지, 동페 좌표계를 이용한 순간전압강하 보상)

  • Lee, Kyo-Sung;Lee, Yong-Jea;Park, Jung-Gyun;Choi, Hyen-Young;Kim, Yang-Mo
    • Proceedings of the KIEE Conference
    • /
    • 2002.07b
    • /
    • pp.973-975
    • /
    • 2002
  • Voltage Sags are the short-duration reductions in rms voltage caused by faults in the electric supply system and the starting of large loads, such as motors. In this paper, we use the dq transformation(dq stationary frame and dq synchronous rotating frame) for series voltage sag compensation algorithm. Analysis, simulation results are presented for voltage sags on a three-phase balanced voltage source.

  • PDF

A development of reliability evaluation model for power plant fan pitch blade control actuator (발전설비 통풍기 날개각 제어작동기 신뢰성평가 모델 개발)

  • Son, Tae-Ha;Huh, Jun-Young
    • Proceedings of the KSME Conference
    • /
    • 2007.05b
    • /
    • pp.3259-3263
    • /
    • 2007
  • This paper describes the proceedings of creating countermeasures after analysis and maintenance be able to conduct operation safely in a power plant. in order to operate the power plant in a stable and reliable way, the best condition of the govemor system can be maintained through the response characteristic analysis of the control device for the pitch blade control hydraulic actuator. The fan pitch blade control hydraulic actuator of a 500MW large-scale boiler is frequently operated under normal operation conditions. Common problems or malfunctions of the pitch blade control hydraulic actuators leads to the decline of boiler thermal efficiency and unexpected power plant trip. The inlet and outlet gas can be controlled by using the fan pitch blade control hydraulic actuator in order to regulate the internal pressure of the furnace and control the frequency in the power plant facility which utilizes soft coals as a power source.

  • PDF

An Estimation of Quantity of Unused Energy of River Water, Seawater and Treated Sewage as Heat Source and Its Availability in Large Facilities (대규모 시설에서 이용가능한 미활용 에너지의 부존량과 그 이용 가능성에 관한 조사연구-하천수.해수.하수처리수를 중심으로)

  • Heo, Jae-Yeong
    • Journal of National Security and Military Science
    • /
    • s.1
    • /
    • pp.423-446
    • /
    • 2003
  • While the demand for energy has shown a sharp increase recently, the supply seems to be limited by the fact that the conventional fossil fuel energy or nuclear energy has its own environmental problems such as, for example, global warming or nuclear waste disposal. To overcome such limited supply of energy, the utilization of natural thermal energy such as river water and seawater as well as treated sewage can be a substantial supplement. The potential use of the unused energy has become more and more feasible these days as the heat pump technology has been advanced. In the present study, the unused energy resources are estimated on regional and monthly basis for each resource by the method proposed here in order to establish the fundamental data for its utilization. The potential use of the unused energy is a1so discussed.

  • PDF

Similarity Detection for Large Scale Software Using Abstracted Source Code (소스코드 요약을 이용한 대규모 소프트웨어 유사도 평가)

  • Park, Seong-Soo;Han, Hwan-Soo
    • Proceedings of the Korean Information Science Society Conference
    • /
    • 2012.06a
    • /
    • pp.39-41
    • /
    • 2012
  • 프로그램 코드의 유사도 측정에 대한 방법은 여러 가지 존재하고 있으며 유사도 측정 프로그램도 많이 존재한다. 이런 프로그램 유사도 측정 도구는 중소규모 소프트웨어 프로젝트에 많이 사용되고 있으나, 실제 대규모 소프트웨어의 유사도 검사를 위해서 사용하기에는 한계가 존재한다. 지금까지 대규모 소프트웨어의 유사도를 측정할 수 있는 객관적 방법이 거의 제시되지 않고 있어, 본 논문에서는 대규모 소프트웨어의 소스코드를 요약하여 서로 다른 프로그램의 유사도를 측정하는 방법을 제시한다.