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Study on Pretreatment Methods for Calcium Extraction from Cuttle Bone (갑오징어갑으로부터 칼슘의 추출을 위한 전처리 방법의 검토)

  • CHO Moon-Lae;HEU Min-Soo;KIM Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.483-487
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    • 2001
  • As a part of basic investigation for utilizing of cuttle bone as a calcium source, we examined on the extraction methods (calcining at $800^{\circ}C$ for 2 hrs, autoclaving at $121^{\circ}C$ for 10 hrs and ultrasonic treatments at $60^{\circ}C$ for 10 hrs) as a pretreatment methods for preparation of calcium-based powder from cuttle bone, The color of calcined calcium-based powder from cuttle bone was white, while that treated by other methods was light yellow. The calcium content in calcined calcium-based powder was $70.5\%$, and revealed high about 2 times compared to those pretreated by other methods. And, calcium solubility in calcined calcium-based powder was improved 22 times compared to raw cuttle bone powder, Among calcining, autoclaving and ultrasonic treatments as a pretreaoent methods for preparation of calcium-based powder from cuttle bone, calcining treatment was superior to other methods on the aspect of color, calcium solubility and purity of calcium-based powder, Judging from X-ray diffraction pattern of calcined calcium-based powder, most of calcium was present as a form of calcium oxide, But, pH of calcined calcium-based powder revealed strong alkali of pH 12.9. This pH value might invoke health risk in using food resource. Therefore, for utilization effectively calcined calcium-based powder from cuttle bone, it requires a suitable treatment such as adjustment of pH to neutral condition.

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Pood Component Characteristics of Cuttle Bone as a Mineral Source (무기질 소재로서 갑오징어갑의 성분 특성)

  • CHO Moon-Lae;HEU Min-Soo;KIM Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.478-482
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    • 2001
  • This study was conducted to get a knowledge on food components of cuttle bone (CB) as a food resource. The yields and ash contents of CB were about $7.5\%$ on whole cuttle fish and about $90\%$ on dry basis, respectively, The contents of heavy metal might not invoke health risk in using food resource, The major mineral of CB was calcium as about $22\%$ in content. The yields, proximate compositions, heavy metal and mineral contents were not significantly different between domestic and imported CB. Judging from X-ray diffraction pattern, most of calcium in CB was present as a form of calcium carbonate (CC), and scanning electron micrograph showed irregular form. Buffering capacity of CB showed strongly at pH 7, and its pattern was the same as shown in that of CC, And CB solution showed a very high degree in turbidity comparing to that of CC solution. The solubility of CB was superior to that of CC, but inferior to those of calcium powders on the market, It was concluded from above results that CB could be effectively utilized as a calcium source.

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Estimation and Application of HU Values for Various Materials as a Function of Physical Factor (물리적 인자의 변화에 따른 다양한 구성물질의 하운스필드 단위 평가 및 응용)

  • Lee, Seung-Wan;Kim, Hee-Joung;Kim, Tae-Ho;Jo, So-Jeong;Lee, Chang-Lae
    • Progress in Medical Physics
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    • v.20 no.3
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    • pp.145-151
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    • 2009
  • This study aims to evaluate CT (Computed Tomography) characteristics through the estimation of HU (Hounsfield Unit) and the corresponding variations using coefficient of variation values for various materials as a function of physical factor. HU values for various materials with varying densities as a function of physical factor were measured using MDCT (Siemens SOMATOM Sensation 4, Germany). The results showed that the HU values were decreased and increased as a function of kVp and material density, respectively. Especially, the HU values for bone and iodine at 140 kVp were 32% and 42% smaller than those at 80 kVp, respectively. In case of iodine, the HU values also decreased and increased as a function of kVp and concentration, respectively. While the HU values were fixed as a function of mAs. The decreased ratio of HU values between 80 keV and 140 keV was different at various concentration and maximum difference was shown as 1.73 at 3% concentration. These results indicated that it may be possible to separate composition of materials, e.g. iodine and bone, using single source CT. The results showed that dual energy techniques using single source CT can be applied to material separation and expand CT imaging techniques to other practical applications.

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Studies on Expression of Cell Cycle Related Genes in HL-60 Cells Undergoing Apoptosis by the Methanol Extract of Hedyotis diffusa (백화사설초 메탄올 추출물에 의한 HL-60 세포(細胞) 고사과정(枯死過程)에서의 cell cycle 관련인자(關聯因子)의 활성변화(活性變化) 연구(硏究))

  • Han, Se-Hee;Lee, Jong-Bum;Moon, Gu;Moon, Suk-Jae;Won, Jin-Hee;Park, Lae-Gil;Lee, Jong-Deok
    • THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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    • v.6 no.1
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    • pp.99-111
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    • 2000
  • Objectives: Hedyotis diffusa is used to treat cancer in traditional Korea Medicine. So this study was carried out to examine the expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa. Methods: 1. HL-60 cells were treated with various concentrations (from 200 to $50{\mu}g/ml$)of methanol extract and H20 extract ($200{\mu}g/ml$) of hedyotis diffusa. After 48 h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with $200{\mu}g/ml$ of methanol extract for the indicated periods. The whole cell lysates were prepared and analyzed by western blotting using anti-p53 antibody. 3. The nuclear extract were prepared and analyzed by western blotting using anti-p21 antibody, anti-p27 antibody, anti-cyclin A antibody, anti-cyclin E antibody and anti-CDK2 antibody. Results: 1. The methanol extract of Hedyotis diffusa induced the death of HL-60 cells in a dose dependent manner. 2. The methanol extract of Hedyotis diffusa makedly decreased the level of p21/Cipl and cyclin A in a time dependent manner. 3. The methanol extract of Hedyotis diffusa markedly increased the level of p27/Kipl and cyclin E in a time dependent manner. 4. The methanol extract of Hedyotis diffusa markedly did not affect the level of CDK2. Conclusions: These results provide evidence that expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa mainly results from decreased level of p21/Cipl and increased level of p27/Kipl of the cell cycle related genes.

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The Implications of the Animation Wall-E on Early Childhood Education (만화영화 <월-이(Wall-E)>가 유아교육에 주는 함의)

  • Kang, Shin Young;Cho, Eun Lae
    • Korean Journal of Childcare and Education
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    • v.8 no.1
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    • pp.85-107
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    • 2012
  • The movie WALL-E shows the Earth which has been overrun with garbage and devoid of plant and animal life, and humans who try to restore the ecosystem. The aim of this study is to find the implications of children's education with a detailed analysis of the movie's theme. The analysis method is the interpretive analysis model that is one of the qualitative methods of data analysis based on the concept of Wolcott. First, the Earth is an abandoned planet covered in trash: the Axiom is a haven for morbidly obese and indolent people. The main area of the earth's destruction, and the captain's cabin is the place to find a clue to awakening and change. Second, with the lapse of time, the Axiom becomes a ship allowing their return to earth. The Earth, recognized as their homeland, turns in to the land of hope when people return. Third, the cleaning robot Wall-E, as a main agent of change, has changed humans as well as itself. The results showed that we found nature-friendly development, destruction of nature, the criticism of modern civilization, the sanctity of life, ecological consciousness, coexistence, symbiosis and a win-win principle in our research.

Analysis of cause of engine failure during power generation using biogas in sewage treatment plant (하수처리장 바이오가스를 이용한 발전시 가스엔진의 고장원인 분석)

  • Kim, Gill Jung;Kim, Lae Hyun
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.13-29
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    • 2016
  • In this study, we analyzed the causes of major faults in the biogas plant through the case of gas engine failure when cogenerating electricity and heat using biogas as a fuel in the actual sewage treatment plant and suggested countermeasures. Hydrogen sulfide in the biogas entering the biogas engine and water caused by intermittent malfunction of the water removal system caused intercooler corrosion in the biogas engine. In addition, the siloxane in the biogas forms a silicate compound with silicon dioxide, which causes scratches and wear of the piston surface and the inner wall of the cylinder liner. The substances attached to the combustion chamber and the exhaust system were analyzed to be combined with hydrogen sulfide and other impurities. It is believed that hydrogen sulfide was supplied to the desulfurization plant for a long period of time because of the high content of hydrogen sulfide (more than 50ppm) in the biogas and the hydrogen sulfide was introduced into the engine due to the decrease of the removal efficiency due to the breakthrough point of the activated carbon in the desulfurization plant. In addition, the hydrogen sulfide degrades the function of the activated carbon for siloxane removal of the adsorption column, which is considered to be caused by the introduction of unremoved siloxane waste into the engine, resulting in various types of engine failure. Therefore, hydrogen sulfide, siloxane, and water can be regarded as the main causes of the failure of the biogas engine. Among them, hydrogen sulfide reacts with other materials causing failure and can be regarded as a substance having a great influence on the pretreatment process. As a result, optimization of $H_2S$ removal method seems to be an essential measure for stable operation of the biogas engine.

Manufacture Lenticular Map of Golf Courses Using Digital Orthophoto (수치정사영상을 이용한 렌티큘러 코스맵 제작)

  • Kim, Kam-Lae;Cheong, Hae-Jin;Cho, Won-Woo
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.5
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    • pp.475-482
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    • 2007
  • Most golfers believe that knowing yardages will improve their score. Certainly it helps with club selection. But, simple "Graphic" yardage guides being notorious for error and inaccuracies, which a serious golfer will pick immediately, only serve to erode the players enjoyment and ultimately, golf course satisfaction. Someone believes with low-level aerial photographic images, golfer will be impressed with the accuracy of the depiction, helping them play a more confident game. But, there are no mapping products in true 3-D available in the world that allows a golfer to determine shot distances in yards or meters. So, we suggest an lenticular technology for real 3-D display as a viable alternative to conventional image map solution. This technology is an image display method for the generation of multi-image effects like 3D visualization or animation. This methodology is cutting edge stereoscopic image which overcomes the limitation of conventional photo tech by recomposing and producing 3 dimensional images. A significant strength of this methods its versatility concerning display effects. The main use of the hardcopy 3-D lenticular displays is in the fields of science, education, planning, and representation. This paper gives a concise overview of the lenticular foil technology and describes the production of the true 3-D yardage book of golf courses. For this study, 3-D effects are achieved and evaluated with the lenticular display by incorporation multiple synthetic images based on digital topographic terrain model and by using the two images of the actual stereopair.

Analysis on Spatiotemporal Variability of Erosion and Deposition Using a Distributed Hydrologic Model (분포형 수문모형을 이용한 침식 및 퇴적의 시.공간 변동성 분석)

  • Lee, Gi-Ha;Yu, Wan-Sik;Jang, Chang-Lae;Jung, Kwan-Sue
    • Journal of Korea Water Resources Association
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    • v.43 no.11
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    • pp.995-1009
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    • 2010
  • Accelerated soil erosion due to extreme climate change, such as increased rainfall intensity, and human-induced environmental changes, is a widely recognized problem. Existing soil erosion models are generally based on the gross erosion concept to compute annual upland soil loss in tons per acre per year. However, such models are not suitable for event-based simulations of erosion and deposition in time and space. Recent advances in computer geographic information system (GIS) technologies have allowed hydrologists to develop physically based models, and the trend in erosion prediction is towards process-based models, instead of conceptually lumped models. This study aims to propose an effective and robust distributed rainfall-sediment yield-runoff model consisting of basic element modules: a rainfall-runoff module based on the kinematic wave method for subsurface and surface flow, and a runoff-sediment yield-runoff model based on the unit stream power method. The model was tested on the Cheoncheon catchment, upstream of the Yongdam dam using hydrological data for three extreme flood events due to typhoons. The model provided acceptable simulation results with respect to both discharge and sediment discharge even though the simulated sedigraphs were underestimated, compared to observations. The spatial distribution of erosion and deposition demonstrated that eroded sediment loads were deposited in the cells along the channel network, which have a short overland flow length and a gentle local slope while the erosion rate increased as rainfall became larger. Additionally, spatially heterogeneous rainfall intensity, dependant on Thiessen polygons, led to spatially-distinct erosion and deposition patterns.

Inhibition of α-Glucosidase by a Semi-Purified Ethyl Acetate Fraction from Submerged-Liquid Culture of Agaricus blazei Murill (신령버섯균사체 액체배양물의 α-glucosidase 저해 효과)

  • Jung, Kwan-Ju;Moon, Yeon-Gyu;Kwon, Jung-Min;Ahn, Chae-Rin;Kim, Jeong-Ok;Ha, Yeong-Lae
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1579-1585
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    • 2011
  • Natural anti-diabetic semipurified ethyl acetate fraction was isolated from the submerged-liquid culture of Agaricus blaze Murill (AB) in a medium containing soybean flakes. Hot-water extract of AB (HEAB) was prepared by extraction at $121^{\circ}C$ for 60 min, followed by filtering through a filter presser filled with diatomate. The ${\beta}$-glucan-free HEAB, which was a supernatant fraction from HEAB by precipitation in an 80% ethanol solution, was fractionated into hexane, chloroform, ethyl acetate, and butanol fractions. The inhibition of the ${\alpha}$-glucosidase activity by fractions was 59.0, 17.0, 61.6, and 37.9%, respectively, suggesting that ethyl acetate fraction was the most active. A subfraction having a strong ${\alpha}$-glucosidase inhibitory activity (80.4%) was isolated from the ethyl acetate fraction. This subfraction contained isoflavones (genistin and daidzin) and their conjugates with sugars as potent inhibiters of ${\alpha}$-glucosidase activity. These results suggest that the ethyl acetate fraction or HEAB containing isoflavones and their sugars conjugates could be useful sources for controlling blood sugar levels in humans.

A Study on the Correlation between Sound Spectrogram and Sasang Constitution (성문(聲紋)과 사상체질(四象體質)과의 상관성(相關性)에 관(關)한 연구(硏究))

  • Yang, Seung-hyun;Kim, Dal Lae
    • Journal of Sasang Constitutional Medicine
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    • v.8 no.2
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    • pp.191-202
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    • 1996
  • Sasang constitution classification is very important subject, so many medical men studied the Sasang constitution classification but there is no certain method to classify objectively. And the purpose of this study is to help classifying Sasang constitution through correlation with sound spectrogram. This study was done it under the suppose that Sasang costitution hag correlation with sound spectrogram. The following results were obtained about correlation between sound spectrogram and Sasang constitution by comparison and analysis the pitch and reading speed of Sasang constitutions; 1. There was a similar tendency in the composition reading speed between taeeumin, soeumin and soyangin. 2. Taeeumin's center was lower measured more than soeumin's and soyangin's in the pitch graph and graph by normal curve fit and there was a similar tendency between soeumin and soyangin. 3. There was a similar tendency in the pitch graph's width between all constitutions. 4. There was a significant difference between taeeumin and soeum in the mean of three constitution's pitch, this means that taeeumin uses lower voice more than soeumin. According to the results, it is considered that there is a correlation between pitch of sound spectrogram and Sasang constitution. And method of Sasang constitution classification through sound spectrogram analysis can be one method as assistant for the objectification of Sasang constitution classification.

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