• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.1843-1847
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• 2010

An efficient method for the preparation of 1,4-disubstituted 1,2,3-triazole compounds is described using polymeric quaternary ammonium salts having azide or alkyne functionality to remove unreacted excess starting molecules (azide/alkyne). Copper metal could easily be removed by simple filtration with a short $Na_2SO_4$/silica cartridge, affording highly regioselective products in high yield and excellent purity without the need for work-up, extraction and chromatographic purification.

• BMB Reports
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• v.48 no.12
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• pp.655-667
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• 2015

Lineage tracing is a widely used method for understanding cellular dynamics in multicellular organisms during processes such as development, adult tissue maintenance, injury repair and tumorigenesis. Advances in tracing or tracking methods, from light microscopy-based live cell tracking to fluorescent label-tracing with two-photon microscopy, together with emerging tissue clearing strategies and intravital imaging approaches have enabled scientists to decipher adult stem and progenitor cell properties in various tissues and in a wide variety of biological processes. Although technical advances have enabled time-controlled genetic labeling and simultaneous live imaging, a number of obstacles still need to be overcome. In this review, we aim to provide an in-depth description of the traditional use of lineage tracing as well as current strategies and upcoming new methods of labeling and imaging.

• Journal of information and communication convergence engineering
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• v.6 no.2
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• pp.204-206
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• 2008

In this paper, we proposed the recognition method that automatically extracts cracks from a surface image acquired by a digital camera and recognizes the directions (horizontal, vertical, -45 degree, and 45 degree) of cracks using the fuzzy single layer perceptron. We compensate an effect of light on a concrete surface image by applying the closing operation, which is one of the morphological techniques, extract the edges of cracks by Sobel masking, and binarize the image by applying the iterated binarization technique. Two times of noise reduction are applied to the binary image for effective noise elimination. After the specific regions of cracks are automatically extracted from the preprocessed image by applying Glassfire labeling algorithm to the extracted crack image, the cracks of the specific region are enlarged or reduced to $30{\times}30$ pixels and then used as input patterns to the fuzzy single layer perceptron. The experiments using concrete crack images showed that the cracks in the concrete crack images were effectively extracted and the fuzzy single layer perceptron was effective in the recognition of the extracted cracks directions.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.679-680
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• 2006

이 논문에서는 가장 많이 알려진 tracking 알고리즘인 Particle-Filter 의 단점을 motion vector 를 기반으로 예측한 sampling 방법과 K-means clustering 을 이용하여 해결하려고 한다. Tracking 에서의 문제는 다중의 유사한 객체들이 merge 후 split 될 때 제대로 추적을 하지 못하고 한 객체만을 추적 한다는 데에 있었다. 그리고 split 되어 객체별로 추적이 가능하더라도 이전에 추적한 객체를 올바로 labeling 하지 못하는 문제가 있다는 것이다. 이 merge-split 문제는 개량된 K-means clustering 을 이용하고, labeling 문제는 motion vector 를 이용한 개량된 sampling 방법으로 개선하였다.

• Archives of Pharmacal Research
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• v.1 no.1
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• pp.27-32
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• 1978

A procedure of $^3H$-radio labeling synthesis for the dammarane triterpene glycosides of Korean ginseng was established by using the ginsenoside $Rg_1$ as starting material. The protons in $C-{11}$ and $C_{13}$ of the aglycone moiety of the glycoside were exchanged with tritium by keto-enol tautomerization of 12-keto-ginsenoside $Rg_1$ which was prepared by partial acetylation, Sarett oxidation and saponification, producing nona-acetate, nonaside $Rg_1$. The acety1-ketone and 12-keto-derivative of ginsenotritated ketone was reduced by metallic sodium and isoproponol to produce the end product $^3H$-ginsenoside $Rg_1$ with 3% radio-chemical recovery in one experiment.

• Applied Microscopy
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• v.51
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• pp.2.1-2.7
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• 2021

Synaptic vesicles, which are endogenous to neurotransmitters, are involved in exocytosis by active potentials and release neurotransmitters. Synaptic vesicles used in neurotransmitter release are reused via endocytosis to maintain a pool of synaptic vesicles. Synaptic vesicles show different types of exo- and endocytosis depending on animal species, type of nerve cell, and electrical activity. To accurately understand the dynamics of synaptic vesicles, direct observation of synaptic vesicles is required; however, it was difficult to observe synaptic vesicles of size 40-50 nm in living neurons. The exo-and endocytosis of synaptic vesicles was confirmed by labeling the vesicles with a fluorescent agent and measuring the changes in fluorescence intensity. To date, various methods of labeling synaptic vesicles have been proposed, and each method has its own characteristics, strength, and drawbacks. In this study, we introduce methods that can measure presynaptic activity and describe the characteristics of each technique.

• Proceedings of the Korean Society of Computer Information Conference
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• 2022.07a
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• pp.447-448
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• 2022

본 논문에서는 대중의 참여를 통해 캔 음료 제품의 점자 표기 실태를 파악하고, 음료 제조사가 이를 개선하도록 유도하는 방안을 제안한다. 캔 음료 상단에 표기된 점자를 촬영한 이미지에서 OpenCV를 통해 점자의 윤곽을 검출하고, 검출된 윤곽의 좌표를 계산하여 점자를 국문으로 번역하는 모듈을 개발한 후 서버에 이식한다. 서버와 통신하는 모바일 애플리케이션을 개발하여 소비자가 점자 이미지를 서버에 업로드하고, 점자의 인식결과를 확인할 수 있도록 한다. 점자 표기가 적절하지 않다고 판단하는 경우 해당 제품에 대한 정보를 기록하도록 하고, 제조사 별로 제보된 횟수의 순위를 제공한다. 이를 통해 소비자는 올바른 점자 표기를 제공하지 않는 제조사를 파악할 수 있으며, 제조사는 이를 의식하고 점자 표기를 개선할 수 있는 효과를 기대한다.

• Proceedings of the Korean Nuclear Society Conference
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• 2001.05a
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• pp.445.1-445
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• 2001

• Journal of Advanced Navigation Technology
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• v.12 no.3
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• pp.280-285
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• 2008

The traditional face detection method is to use difference picture method are used to detect movement. However, most do not consider this mathematical approach using real-time or real-time implementation of the algorithm is complicated, not easy. This paper, the first to detect real-time facial image is converted YCbCr and RGB video input. Next, you convert the difference between video images of two adjacent to obtain and then to conduct Glassfire Labeling. Labeling value compared to the threshold behavior Area recognizes and converts video extracts. Actions to convert video to conduct face detection, and detection of facial characteristics required for the extraction and use of AdaBoost algorithm.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.7
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• pp.1257-1262
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• 2001

In recent years there has been a lot of interest in carrying IP over WDM networks in an efficient manner. The benefits here include larger bandwidth capacities, better network scalability, and more efficient operation. W based approach, termed "lambda-labeling" is presented for direct If over WDM integration. In this paper, we study on architecture approach method consider of optical Internet evolution that based on MPLamdaS conception of IETF. Label stack conception collect electronic LSP of optical LSP. This paper is proposed method of co-operation between MPLS domain and MPLambdaS domain. Additionally, proposed architecture of Edge Optical LSR.tical LSR.