• 한국공간구조학회논문집
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• 제8권4호
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• pp.65-71
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• 2008

본 논문은 유닛 모듈러를 구성하는 주구조체인 각형강관 기둥과 냉간성형 LEB C-형강 보가 볼트 접합된 접합부를 가진 모듈러 건물의 시공성을 평가하는 것이 연구의 목적이다. 모듈러 건축의 장점은 공사기간 단축, 경량성, 이동가능성 등으로 볼 수 있다. 반면에 유닛 또는 모듈의 운송비용이 공사비 절감 비용을 반감시킬 수 있고 많은 공장들이 외부 지역에 있으므로 이들을 도심지나 원하는 지역으로 이동하기 위해서는 비용이 추가된다. 그리고 유닛이나 모듈 설치에 크레인 등의 장비 사용으로 인해 시공비용이 증가될 수 있는 단점들이 존재한다. 본 연구에서는 앞서 연구한 모듈형상에 근거하여 해체조립이 용이한 모듈러 건축물의 시공사례를 통하여 공기, 비용 등 측면에서 기존 연구와 비교분석을 진행하여 모듈러 건물의 시공성을 평가하고자 한다.

• BMB Reports
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• 제49권7호
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• pp.394-399
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• 2016

Glioma-Associated Oncogene Homolog1 (Gli1) is known to be activated in malignant glioma; however, its downstream pathway has not been fully explained. The aim of this study was to explore the role of Gli1-Aquaporin1 (AQP1) signal pathway in glioma cell survival. Our data suggests that both Gli1 and AQP1 are upregulated in glioma tissues, as in comparison to in normal tissues. These up-regulation phenomena were also observed in glioma U251 and U87 cells. It was demonstrated that Gli1 positively regulated the AQP1 expression. By luciferase reporter gene and ChIP assay, we observed that this modulation process was realized by combination of Gli1 with AQP1 promotor. In addition, knock down of Gli1 by siRNA interference reduced the viability of glioma cells as well as suppressed cell metastasis. Also, the inhibitory effects of cell survival by silenced Gli1 were abrogated by AQP1 overexpression. In summary, glioma cell survival is a regulatory process and can be mediated by Gli1-AQP1 pathway.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4007-4011
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• 2012

Purpose: Retinoblastoma-interacting zinc finger gene (RIZ1) is a tumor suppressor gene which is highly inactivated by promoter hypermethylation in patients with liver fluke-related cholangiocarcinoma (CCA). Epigenetic aberration of this gene might withdraw the ability to restrain tumor cell proliferation and migration. We aimed to define the role of RIZ1 on cell proliferation and migration in CCA cell line. Materials and methods: Small interference RNA (siRNA) was used to knock down the expression of RIZ1 in a CCA-derived cell line in which cell proliferation and cell migration were performed. Results: A predominant nuclear localization of RIZ1 was observed. Reduction of RIZ1 by siRNA augmented cell proliferation and migration. Conclusion: The result suggested that RIZ1 might play a role in regulating cell proliferation and migration in CCA. Reduction of RIZ1 expression may aggravate the progression of CCA.

• BMB Reports
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• 제41권6호
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• pp.455-460
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• 2008

Calcineurin (Cn) is a serine/threonine phosphatase implicated in a wide variety of biological responses. To identify proteins that mediate Cn signaling pathway effects, we used yeast two-hybrid assays to screen for Cn interacting proteins, discovering a protein encoded by the gene, cnp-2 (Y46G5A.10). Utilizing serially deleted forms of Cn as baits, we demonstrated that the catalytic domain of Cn (TAX-6) binds with CNP-2, and this physical interaction was able to be reconstituted in vitro, supporting our yeast two-hybrid results. cnp-2 is a nematode-specific novel gene found in C. elegans as well as its closest relative, C. briggsae. CNP-2 was strongly expressed in the intestine of C. elegans. To study the function of cnp-2, we performed cnp-2 RNAi knock-down and characterized phenotypes associated with Cn mutants. However, no gross defects were revealed in these RNAi experiments. CNP-2 was proven to be a Cn binding protein; however, its role remains to be elucidated.

• 한국동물생명공학회지
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• 제34권4호
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• pp.318-321
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• 2019

Recent studies showed that tight junctions (TJs) integrity and assembly are required for blastocyst development in mouse and pig models. However, the biological functions of TJs associated with embryo implantation and maintenance of pregnancy were not investigated yet. To examine whether disrupted TJs affect further embryo development, we employed RNAi approach and inhibitor treatment. The embryos were injected with Cxadr (Coxsackievirus and adenovirus receptor) siRNA for knock down (KD) and treated with Adam10 (A Disintegrin and Metalloproteinase specific inhibitor 10; GI254023X; SI). We compared blastocyst development and paracellular sealing assay using FITC dextran uptake between control and KD or SI embryos. Finally, we transferred control and Cxadr KD or Adam 10 SI treated blastocyst to uteri of recipients. Cxadr KD and Adam 10 SI showed lower blastocyst development and more permeable to FITC-dextran. Moreover, we observed that half of KD and inhibited embryos failed to maintain pregnancies after the second trimester. Our findings suggested that TJs integrity is required for the maintenance of pregnancy and can be used as a selective marker for the successful application of assisted reproduction technologies.

• Reproductive and Developmental Biology
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• 제40권2호
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• pp.23-26
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• 2016

Transcription factor called activating enhancer binding protein 2C (AP2-gamma) is found in a variety of species and expressed from oocyte stage onwards, particularly restricted to the trophectoderm. Recent studies demonstrated that ablation of Tfap2c led to failure of tight junction biogenesis, particularly the knock-down embryos of Tfap2c did not form cavity from morula to blastocyst in mouse and pig. We speculated that the Tfa2pc may also be involved in desmosome biogenesis because blastocoel formation is coincident with the establishment of desmosome. To determine this, we depleted Tfap2c injecting siRNA into one-cell zygote and analysed the expression levels of genes that are required for desmosome complex such as PkP2, Pkp3, Dsc2, and Dsg2. We found only Pkp3 was up-regulated in the knockdowned morula embryos. Interestingly, upstream region of Pkp3 had putative Tfap2c binding sites. In conclusion, our results suggest that Tfap2c is not a crucial factor but somehow it might be involved in desmosome biogenesis directly or indirectly via Pkp3.

• The Plant Pathology Journal
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• 제32권4호
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• pp.371-376
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• 2016

Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.

• 안전기술
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• 제187호
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• pp.19-21
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• 2013

현대글로비스는 현대자동차 그룹 계열 종합물류유통기업으로 첨단 정보시스템과 선진 물류기술을 활용해 국내 국제물류, 해상운송, 물류컨설팅 등의 업무를 수행하는 전문업체이다. 또한 최근에는 물류는 물론 유통, 자원개발 및 자원순환을 활용한 친환경 경영 인프라를 구축해나가고 있다. 이번에 소개되는 현대글로비스 아산KD센터는 현대글로비스의 핵심 사업이라 할 수 있는 해외공장 자동차 조립생산용 부품(KD: KNOCK DOWN) 공급사업을 전문으로 하는 사업장이다. 2004년 11월 현대자동차 아산공장 인근 인주지방산업단지 내에 준공된 현대글로비스 아산KD센터는 임직원 50명과 협력사 인원 300명 등 총 350영의 인력을 갖추고 KD사업의 본산지로 자리매김해 나가고 있다. 실제로 이곳 센터는 발주에서 포장, 운송은 물론 현지에서의 내륙운송 및 보관 등 KD물류에 대한 종합서비스를 제공하고 있다. 이처럼 현대글로비스 아산KD센터는 자동차조립생산용 부품을 국내외 협력사로부터 조달해 전 세계로 운송 판매하고 있는 만큼 근로자의 안전보건이 기업경영의 초석이 되고 있다. 여기에 더해 세계 자동차산업의 경쟁력을 좌우하는 부품 물류산업을 선도하는 기업인만큼 이곳의 안전관리는 전국 안전인들의 남다른 관심을 받고 있다. 근로자의 안전보건을 밑바탕으로 글로벌 물류기업으로 비상하고 있는 현대글로비스 아산KD센터 찾아가봤다.

• Molecules and Cells
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• 제38권10호
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• pp.904-910
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• 2015

Negative regulator of reactive oxygen species (NRROS) is known to repress ROS generation in phagocytes. In this study, we examined the roles of NRROS in both osteoclasts and osteoblasts. Our results demonstrate that NRROS negatively regulates the differentiation of osteoclasts, but not osteoblasts. Further, overexpression of NRROS in osteoclast precursor cells attenuates RANKL-induced osteoclast differentiation. Conversely, osteoclast differentiation is enhanced upon siRNA-mediated knock-down of NRROS. Additionally, NRROS attenuates RANKL-induced $NF-{\kappa}B$ activation, as well as degradation of the NOX1 and NOX2 proteins, which are required for ROS generation. Based on our observations, we present NRROS as a novel negative regulator of RANKL-induced osteoclastogenesis.

• IMMUNE NETWORK
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• 제11권6호
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• pp.364-367
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• 2011

Background: Prostaglandins (PGs) play pathogenic and protective roles in inflammatory diseases. The novel concept of PGs as immune modulators is being documented by several investigators. By establishing an in vitro experimental model containing human follicular dendritic cell-like cells, HK cells, we reported that HK cells produce prostaglandin $E_2$ ($PGE_2$) and prostaglandin $I_2$ ($PGI_2$) and that these PGs regulate biological functions of T and B cells. Methods: To investigate the respective contribution of cyclooxygenase-1 (COX-1) and COX-2 to $PGE_2$ and $PGI_2$ production in HK cells, we performed siRNA technology to knock down COX enzymes and examined the effect on PG production. Results: Both $PGE_2$ and $PGI_2$ productions were almost completely inhibited by the depletion of COX-2. In contrast, COX-1 knockdown did not significantly affect PG production induced by lipopolysaccharide (LPS). Conclusion: The current results suggest that mPGES-1 and PGIS are coupled with COX-2 but not with COX-1 in human follicular dendritic cell (FDC) and may help understand the potential effects of selective COX inhibitors on the humoral immunity.