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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Novel calcineurin interacting protein-2: the functional characterization of CNP-2 in Caenorhabditis elegans

  • Xianglan, Cai (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Ko, Kyung-Min (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Singaravelu, Gunasekaran (Department of Life Science, Hanyang University) ;
  • Ahnn, Joo-Hong (Department of Life Science, Hanyang University)
  • Received : 2008.01.17
  • Accepted : 2008.03.07
  • Published : 2008.06.30
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Abstract

Calcineurin (Cn) is a serine/threonine phosphatase implicated in a wide variety of biological responses. To identify proteins that mediate Cn signaling pathway effects, we used yeast two-hybrid assays to screen for Cn interacting proteins, discovering a protein encoded by the gene, cnp-2 (Y46G5A.10). Utilizing serially deleted forms of Cn as baits, we demonstrated that the catalytic domain of Cn (TAX-6) binds with CNP-2, and this physical interaction was able to be reconstituted in vitro, supporting our yeast two-hybrid results. cnp-2 is a nematode-specific novel gene found in C. elegans as well as its closest relative, C. briggsae. CNP-2 was strongly expressed in the intestine of C. elegans. To study the function of cnp-2, we performed cnp-2 RNAi knock-down and characterized phenotypes associated with Cn mutants. However, no gross defects were revealed in these RNAi experiments. CNP-2 was proven to be a Cn binding protein; however, its role remains to be elucidated.

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References

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