• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6245-6248
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• 2013

Aims: To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferation and metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore related mechanisms. Methods: SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cell proliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzed using Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR and Western blotting. Results: Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells, compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expression levels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreated levels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNA group was obviously down-regulated. The numbers of migrating cells ($51.38{\pm}4.71$) in the PTTG siRNA group was obviously lower than that in untreated group ($131.33{\pm}6.12$) and the control siRNA group ($127.72{\pm}5.20$) (P < 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down may be closely correlated with down-regulation of MMP-2 and MMP-9 expression. Conclusion: Inhibition of PTTG expression may be a new target for therapy of CSCC.

• Journal of Life Science
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• v.26 no.2
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• pp.174-180
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• 2016

Dicumarol is a coumarin derivative isolated from sweet clover (Melilotus alba), and has anti-coagulant activity with the inhibitory activity of NAD(P)H quinone oxidoreductase1 (NQO1). NQO1 catalyzes the two-electron reduction of quinones to hydroquinones. Dicumarol competes with NAD(P)H for binding to NQO1, resulting in the inhibition of NQO1 enzymatic activity. The expression of matrix metalloproteinases (MMPs) has been implicated in the invasion and metastasis of cancer cells. The expression of MMPs is regulated by cytokines and signal transduction pathways, including those activated by phorbol myristate acetate (PMA). However, the effects of dicumarol on metalloproteinase (MMP)-9 expression and activity are not investigated here. This study investigated whether dicumarol inhibits MMP-9 expression and activity in PMA-treated human renal carcinoma Caki cells. Dicumarol markedly inhibited the PMA-induced MMP-9 mRNA expression and MMP-9 activity. NF-κB and AP1 promoter activity, which is important in MMP-9 expression, also decreased in dicumarol-treated cells. Furthermore, dicumarol markedly suppressed the ability of PMA-mediated migration in Caki cells. When the relevance of NQO1 in the dicumarol-mediated inhibitory effect on PMA-induced MMP9 activity was elucidated, knock-down of NQO1 with siRNA was found to have no effect on PMA-induced MMP9 activity, suggesting that the stimulating effect of dicumarol on PMA-induced MMP9 activity is independent of NQO1 activity. Taken together, the present studies suggested that dicumarol may inhibit PMA-induced migration via down-regulation of MMP-9 expression and activity.

• BMB Reports
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• v.49 no.7
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• pp.388-393
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• 2016

Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.944-949
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• 2017

Objective: Investigated the effect and mechanism of ascorbic acid on the development of porcine embryos produced by somatic cell nuclear transfer (SCNT). Methods: Porcine embryos were produced by SCNT and cultured in the presence or absence of ascorbic acid. Ten-eleven translocation 3 (TET3) in oocytes was knocked down by siRNA injection. After ascorbic acid treatment, reprogramming genes were analyzed by realtime reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, relative 5-methylcytosine and 5-hydroxymethylcytosine content in pronucleus were detected by realtime PCR. Results: Ascorbic acid significantly increased the development of porcine embryos produced by SCNT. After SCNT, transcript levels of reprogramming genes, Pou5f1, Sox2, and Klf were significantly increased in blastocysts. Furthermore, ascorbic acid reduced 5-methylcytosine content in pronuclear embryos compared with the control group. Knock down of TET3 in porcine oocytes significantly prevents the demethylation of somatic cell nucleus after SCNT, even if in the presence of ascorbic acid. Conclusion: Ascorbic acid enhanced the development of porcine SCNT embryos via the increased TET3 mediated demethylation of somatic nucleus.

• Journal of the Korea Furniture Society
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• v.19 no.1
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• pp.33-43
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• 2008

This study intended to explore trends of furniture sold in general merchandise stores such as Lotte mart, E-mart, GSmart, Home ever, and Home plus, which are run by conglomerates in Korea. Through internet, related books and papers, interviews with the mart-related people, styles, items, prices, manufacturers, and materials of furniture well sold in the marts were researched and analyzed qualitatively. As the result of the analysis, the furniture expressed the scale to be able to put in the cart of the marts, knock-down and folding structure to be easy to disassemble, pack, and move, low prices below one hundred thousand won, the light materials like plastic, aluminum and the more MDF than hard wood. Each item was made of PB reflecting lower prices of 10 to 20 percentage for the consumer.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2007.05a
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• pp.110-113
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• 2007

Modernity which is superficial phenomenon set off the mass scale for mass consumption and provide uniformly artificial environment. But natural destruction, environment pollution, resources exhaustion and so on has been caused by this and now ecology is threatened by destruction and damage beyond the limitation and human beings survival is even threatened. Accordingly furniture development for environment preservation considered environment problem is the urgent real situation. Recent paradigm is the concept of Eco-design which is the green design possible to live together in symbiosis, and new types of alternative furniture are needed in Korea as well. 'Furniture for disassembly' is presented as new method for alternative furniture.

• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1291-1299
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• 2014

Recently, multifunctional nanomaterials have been developed as nanotherapeutic agents for cellular imaging and targeted cancer treatment because of their ease of synthesis and low cytotoxicity. In this study, we developed a multifunctional, two-component nanorod consisting of gold (Au) and nickel (Ni) blocks that enables dual-fluorescence imaging and the targeted delivery of small interfering RNA (siRNA) to improve cancer treatment. Fluorescein isothiocyanate-labeled luteinizing hormone-releasing hormone (LHRH) peptides were attached to the surface of a Ni block via a histidine-tagged LHRH interaction to specifically bind to a breast cancer cell line, MCF-7. The Au block was modified with TAMRA-labeled thiolated siRNA in order to knock down the vascular endothelial growth factor protein to inhibit cancer growth. These two-component nanorods actively targeted and internalized into MCF-7 cells to induce apoptosis through RNA interference. This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment, with capabilities in dual imaging and targeted gene delivery.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2000.04a
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• pp.17-20
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• 2000

가전업의 CKD(Complete Knock Down)업무는 해외의 바이어나 현지공장에 가전제품을 구성하는 부품/반제품들을 개별 포장하여 수출하는 물류업무이다. 이러한 CKD 업무는 가격경쟁이 치열한 부분으로 경쟁력 강화를 위해 물류비의 절감이 요구되고 있다. 특히 CKD 업무의 경쟁력 강화를 위해서는 포장품질과 적재율 향상을 위한 포장/적재의 최적화가 이루어져야 하며, 이를 지원하는 Packing Optimization System 의 구축이 절실히 필요하다. 본 논문에서는 가전업 CKD의 업무특징과 업무환경의 변화에 대해 기술하였으며, 환경변화에 대한 대응방법으로 가전업 CKD 업무를 위한 Packing Optimization System 의 주요 기능과 업무 프로세스를 제시하였다. 그리고, 시뮬레이션 방법을 이용한 Packing Optimization system의 구현 사례를 예시하였다.

• 한국공간정보시스템학회:학술대회논문집
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• 2004.05a
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• pp.83-88
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• 2004

First author proposed a proportioning method for member sections of a single layer reticulated dome subjected to uniform and non-uniform load without any geometrical initial imperfection, and discussed the validity and effectiveness of the method which was based on linear buckling stress and a knock down factor. However, buckling of a single layer reticulated dome is strongly affected by initial imperfection. It is well known that geometrical initial imperfections reduce the nonlinear buckling capacity of a single layer raticulated dome. Thus, structural engineers may be recommended to reflect the effects of geometrical initial imperfections in proportioning member sections. In this paper, firstly, the presented proportioning method by first author is applied to dome without consideration of any imperfections and the thickness and diameter of each member are determined. Secondly, the load bearing capacities of the proportioned domes are checked with the imperfection, by the inelastic buckling analysis.

• Journal of the Korea Furniture Society
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• v.16 no.2 s.30
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• pp.67-76
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• 2005

Bauhaus, founded in Weimar, Germany in 1919, represented Modernism which is proven in the field of art as well as architecture. Furthermore, in design field, Bauhaus is the essential historical event whose influence continues in contemporary design. Bauhaus furniture is simple and austere and has linear shapes which are suitable for mass production. Bauhaus developed functional furniture design such as knock-down and pre-fabricated furniture. In terms of finishes, Bauhaus furniture epitomizes the influence of De Stijl which used either achromatic colors and three primary colors or natural finishes of wood. Bauhaus contributed to furniture design field as below. 1. Success in mass-produced furniture through standardization and pre-fabrication 2. Pioneered on industrialization through collaboration between academia and industry 3. played essential role developing modem furniture by using not only metal but also such diverse materials as plywood, glass, and leather