• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.1029-1033
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• 2012

A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the "PTPome." In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.

• 한국표면공학회지
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• 제38권3호
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• pp.89-94
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• 2005

We investigate the reaction stability of cobalt and nickel with side-wall materials of $SiO_2\;and\;Si_3N_4$. We deposited 15nm-Co and 15nm-Ni on $SiO_2(200nm)/p-type$ Si(100) and $Si_3N_4(70 nm)/p-type$ Si(100). The samples were annealed at the temperatures of $700\~1100^{\circ}C$ for 40 seconds with a rapid thermal annealer. The sheet resistance, shape, and composition of the residual materials were investigated with a 4-points probe, a field emission scanning electron microscopy, and an AES depth profiling, respectively. Samples of annealed above $1000^{\circ}C$ showed the agglomeration of residual metals with maze shape and revealed extremely high sheet resistance. The Auger depth profiling showed that the $SiO_2$ substrates had no residual metallic scums after $H_2SO_4$ cleaning while $Si_3N_4$ substrates showed some metallic residuals. Therefore, the $SiO_2$ spacer may be appropriate than $Si_3N_4$ for newly proposed Co/Ni composite salicide process.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.145-150
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• 2002

Biotechnology in the 21st century will be driven by three emerging technologies: genomics, high-throughput biology, and bioinformatics. These technologies are complementary to one another. A large number of economically important crops are currently subjected to whole genome sequencing. Functional genomics for determining the functions of the genes comprising the given plant genome is under progress by using various means including phenotyping data from transgenic mutants, gene expression profiling data from DNA microarrays, and metabolic profiling data from LC/mass analysis. The aim of plant molecular breeding is shifting from introducing agronomic traits such as herbicide and insect resistance to introducing quality traits such as healthful oils and proteins, which will lead to improved and nutritional food and feed products. Plant molecular breeding is also expected to aim to develop crops for producing human therapeutic and industrial proteins.

• 한국표면공학회지
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• 제48권5호
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• pp.233-237
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• 2015

In order to investigate the potassium (K) gettering, Al-1%Si/$SiO_2$/PSG multilevel thin films were fabricated. Al-1%Si thin films and $SiO_2$/PSG passivations were deposited by using DC magnetron sputter techniques and APCVD (atmosphere pressure chemical vapor deposition), respectively. Heat treatment was carried out at $300^{\circ}C$ for 5 h in air. SIMS (secondary ion mass spectrometry) depth profiling analysis was used to determine the distribution of K, Al, Si, P, and other elements throughout the $SiO_2$/PSG passivated Al-1%Si thin film interconnections. Potassium peaks were observed throughout the $SiO_2$/PSG passivation layers, and especially the interface gettering at the $SiO_2$/PSG and at the Al-1%Si/$SiO_2$ interfaces was observed. Potassium gettering in Al-1%Si/$SiO_2$/PSG multilevel thin films is considered to be caused by a segregation type of gettering.

• 한국해양공학회지
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• 제22권2호
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• pp.50-57
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• 2008

The effect of weld improvement on the corroded fatigue life of welded structures was investigated. Toe grinding, TIG dressing and weld profiling were used as the geometric improvement methods. Fatigue tests under the corroded condition in artificial seawater were carried out to investigate the corrosion fatigue behavior of API 2W Gr.50T steel plate produced by POSCO. The test results in weld improved conditions were compared with those in as-welded condition. The test results were also compared with the design curves in UK DEn Class F. Corroded fatigue life of weld improved specimens was longer than that of as-welded specimen. Especially, the corroded fatigue life exceeded the mean SN curve in air of UK DEn Class F.

• 로봇학회논문지
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• 제16권2호
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• pp.94-102
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• 2021

This study addresses a method for 3D reconstruction using acoustic data with heterogeneous sonar devices: Forward-Looking Multibeam Sonar (FLMS) and Profiling Sonar (PS). The challenges in sonar image processing are perceptual ambiguity, the loss of elevation information, and low signal to noise ratio, which are caused by the ranging and intensity-based image generation mechanism of sonars. The conventional approaches utilize additional constraints such as Lambertian reflection and redundant data at various positions, but they are vulnerable to environmental conditions. Our approach is to use two sonars that have a complementary data type. Typically, the sonars provide reliable information in the horizontal but, the loss of elevation information degrades the quality of data in the vertical. To overcome the characteristic of sonar devices, we adopt the crossed installation in such a way that the PS is laid down on its side and mounted on the top of FLMS. From the installation, FLMS scans horizontal information and PS obtains a vertical profile of the front area of AUV. For the fusion of the two sonar data, we propose the probabilistic approach. A likelihood map using geometric constraints between two sonar devices is built and a monte-carlo experiment using a derived model is conducted to extract 3D points. To verify the proposed method, we conducted a simulation and field test. As a result, a consistent seabed map was obtained. This method can be utilized for 3D seabed mapping with an AUV.

• 교육시설 논문지
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• 제26권5호
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• pp.3-10
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• 2019

This study has a purpose to profile local sectors into meaningful groups by using facilities rates of Social Overhead Capital(SOC) for daily life. Comparing SOC for daily life among the meaningful groups, the profiling and comparison results bring the comprehensive understanding about the educational facilities in local sectors. For the research purpose, this study utilized Latent Profile Analysis(LPA) by using variables such as population, road information, SOC for daily life, usage of land, possession of land, and appraised value of land from the 2018 Geographic Information System(GIS) dataset of Gwangjin-gu, where is one of the administrative district of Seoul City. Results showed that there are four latent groups of sectors among 904 local sectors(100 squared-meters sector per each) in Gwangjin-gu. By comparing the four latent groups by using LPA, the results diagnose each sector's status and help to improve the policy about educational facilities. Specifically, by using dataset for SOC of daily life, there are four groups of local sectors and each group has different features. Based on the different features of local sector groups, there can be improved management of educational facilities matching with each group's features.

• 정보보호학회논문지
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• 제19권4호
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• pp.115-124
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• 2009

사이버범죄는 인터넷 가상공간이 사회 전반으로 광범위하게 확대되면서 공격 기술력을 과시하려던 의도에서 최근에는 영리와 일반적인 목적의 범죄로 변화하고 있다. 신택스(Syntax) 기반의 탐지 분석이 가능한 네트워크 및 시스템 공격이 아닌 시맨틱(Semantic) 기반으로 수동적인 탐지와 대응을 요하는 콘텐츠 이상의 공격에 의한 인터넷 사기와 명예훼손, 개인정보 침해, 저작권 침해 등이 일어나고 있다. 시맨틱 기반의 범죄는 컴퓨터 이용자, 즉 인간의 내면적인 취약성을 이용하는 심리적 요인에 근거한다. 이 논문에서는 공개된 사이버범죄를 분석 후 사회적 영향력과 기술적 영향 사이버범죄의 심리적 관점에서의 프로파일링 방안을 연구하여 사이버범죄를 그 발생 수에 의하여 분류하였고, 이를 통하여 결국 범죄의 분석과 대응에 소요되는 시간을 줄이는 계기가 될 수 있음을 보이고 있다.

• 대한의용생체공학회:의공학회지
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• 제42권5호
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• pp.232-240
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• 2021

Heterogeneity in cancer is the major obstacle for precision medicine and has become a critical issue in the field of a cancer diagnosis. Many attempts were made to disentangle the complexity by molecular classification. However, multi-dimensional information from dynamic responses of cancer poses fundamental limitations on biomolecular marker-based conventional approaches. Cell morphology, which reflects the physiological state of the cell, can be used to track the temporal behavior of cancer cells conveniently. Here, we first present a hybrid learning-based platform that extracts cell morphology in a time-dependent manner using a deep convolutional neural network to incorporate multivariate data. Feature selection from more than 200 morphological features is conducted, which filters out less significant variables to enhance interpretation. Our platform then performs unsupervised clustering to unveil dynamic behavior patterns hidden from a high-dimensional dataset. As a result, we visualize morphology state-space by two-dimensional embedding as well as representative morphology clusters and trajectories. This cell morphology profiling strategy by hybrid learning enables simplification of the heterogeneous population of cancer.

• Journal of Korean Dental Science
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• 제16권1호
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• pp.35-46
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• 2023

Purpose: Culture-based methods for microbiological diagnosis and antibiotic susceptibility tests have limitations in the management of orofacial infections. We aimed to profile pus microbiota and identify antibiotic resistance genes (ARGs) using a culture-independent approach. Materials and Methods: Genomic DNA samples extracted from the pus specimens of two patients with orofacial abscesses were subjected to shotgun sequencing on the NovaSeq system. Taxonomic profiling and prediction of ARGs were performed directly from the metagenomic raw reads. Result: Taxonomic profiling revealed obligate anaerobic polymicrobial communities associated with infections of odontogenic origins: the microbial community of Patient 1 consisted of one predominant species (Prevotella oris 74.6%) with 27 minor species, while the sample from Patient 2 contained 3 abundant species (Porphyromonas endodontalis 33.0%; P. oris 31.6%; and Prevotella koreensis 13.4%) with five minor species. A total of 150 and 136 putative ARGs were predicted in the metagenome of each pus sample. The coverage of most predicted ARGs was less than 10%, and only the CfxA2 gene identified in Patient 1 was covered 100%. ARG analysis of the seven assembled genome/metagenome datasets of P. oris revealed that strain C735 carried the CfxA2 gene. Conclusion: A metagenomics-based approach is useful to profile predominantly anaerobic polymicrobial communities but needs further verification for reliable ARG detection.