• Title/Summary/Keyword: javanica

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Cytochrome Oxidase Subunit II (COII) Sequence Analysis of Root-knot Nematode, Meloidogyne sp. HSC, Infesting Yam (Dioscorea bulbifera) (둥근마(Dioscorea bulbifera)를 가해하는 뿌리혹선충(Meloidogyne sp. HSC)의 Cytochrome Oxidase Subunit II (COII) 염기서열 분석)

  • Han, Sang-Chan;Kang, Sang-Jin;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.169-173
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    • 2007
  • Root-knot nematode damage was found on yam, Dioscorea bulbifera in Andong Korea. From the root-knots, female nematodes were isolated and subjected to DNA sequence analysis. Sequence of cytochrome oxidase subunit II (COII) was analyzed from the genomic DNA of the isolate. COII locus size and sequence of the nematode isolate were similar to those of Meloidogyne javanica or M. incognita. However, an analysis of HinfI restriction site, a species-specific character between these two species, showed that the isolate did not match to either M. javanica or M. incognita.

Chemical Components from the Stem Bark of Rhus javanica L. (붉나무 수피의 성분)

  • Chung, Sun-Chae;Hwang, Bang-Yeon;Oh, Gab-Jin;Kang, Shin-Jung;Kim, Mi-Jeong;Choi, Woo-Hoi;Lee, Kyong-Soon;Ro, Jai-Seup
    • Korean Journal of Pharmacognosy
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    • v.30 no.3
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    • pp.295-300
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    • 1999
  • Seven compounds were isolated from the n-BuOH extract of the dried stem barks of Rhus javanica L. On the basis of physico-chemical, spectroscopic evidences and comparison with authentic samples, the compounds $1{\sim}7$ were identified as gallic acid (1), methyl gallate (2), scopoletin (3), scopolin (4), $1,\;2,\;3,\;4,\;6-penta-O-galloyl-{\beta}-D-glucose$ (5), orcinol (6) and $orcinol-{\beta}-D-glucoside$ (7). Among these compounds, scopolin, $orcinol-{\beta}-D-glucoside$ were isolated from this plant for the first time.

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Taxonomic reexamination of two Huperzia species (Lycopodiaceae) in Northeast Asia (동북아시아 뱀톱속 (석송과) 두 종의 분류학적 재검토)

  • Lim, Jina;Yun, Na Rae;Lee, Byoung-yoon;Sun, Byung-Yun
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.299-305
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    • 2015
  • A comparison of the external morphology and spores clearly distinguished Huperzia lucidula (Michaux) Trevis. in northeast Asia and North America in terms of leaf shape, leaf margin, distribution of stomata on leaves, and spore shape. Therefore, the northeast Asian plants should be treated as a separate species, H. asiatica (Ching) B.-Y. Sun & J. Lim. In addition, we believe that H. javanica (Sw.) C. Yang, regarded as conspecific or infraspecific with H. serrata (Thunb.) Trevis., is a distinct species based on the presence of a leaf petiole and serrated leaf margins, and the shape of the gemmiferous branchlets.

Expression of recombinant protein from Oenanthe javanica DC. leaf tissues as a biofactory (Biofactory로서의 미나리 엽조직을 이용한 재조합단백질 발현)

  • Shin, Dong-Il;Park, Hee-Sung
    • KSBB Journal
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    • v.23 no.6
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    • pp.554-556
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    • 2008
  • Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of $OD_{600}=0.5$ to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.

Dual Biocontrol Potential of the Entomopathogenic Fungus, Isaria javanica, for Both Aphids and Plant Fungal Pathogens

  • Kang, Beom Ryong;Han, Ji Hee;Kim, Jeong Jun;Kim, Young Cheol
    • Mycobiology
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    • v.46 no.4
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    • pp.440-447
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    • 2018
  • Dual biocontrol of both insects and plant pathogens has been reported for certain fungal entomopathogens, including Beauveria bassiana and Lecanicillum spp. In this study, we demonstrate, for the first time, the dual biocontrol potential of two fungal isolates identified by morphological and phylogenetic analyses as Isaria javanica. Both these isolates caused mortality in the greater wax moth, and hence can be considered entomopathogens. Spores of the isolates were also pathogenic to nymphs of the green peach aphid (Myzus persicae), with an $LC_{50}$ value of $10^7spores/mL$ 4 days after inoculation and an $LT_{50}$ of 4.2 days with a dose of $10^8spores/mL$. In vitro antifungal assays also demonstrated a strong inhibitory effect on the growth of two fungi that are pathogenic to peppers, Colletotrichum gloeosporioides and Phytophthora capsici. These results indicate that I. javanica isolates could be used as novel biocontrol agents for the simultaneous control of aphids and fungal diseases, such as anthracnose and Phytophthora blight, in an integrated pest management framework for red pepper.

Antimicrobial Activity of Extracts from Medicinal Herbs Against Escherichia coli (Escherichia coli에 대한 한약재의 항균활성)

  • Chang, Hyung-Soo;Choi, Il
    • The Korean Journal of Community Living Science
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    • v.18 no.2
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    • pp.293-300
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    • 2007
  • The antimicrobial activity of 10 different traditional medicinal herbs extracts against E. coli was determined using paper disc method. Caesalpinia sappan and Rhus javanica, extracts in 5 mg/ml, and Paeonia suffruticosa and Seutellaria baicalensis extracts in 10 mg/ml showed significant antimicrobial activity against E. coli. Minimum inhibitory concentrations of medicinal herbs extracts were in the range of $1.4{\sim}8mg/ml\;and\;1.2{\sim}12mg/ml$, for MeOH extracts and EtOH extracts, respectively. In addition, the antimicrobial activity of each solvent fraction was most significant with the EtOAc layer. Optical density at 620nm after 24 hours incubation of E. coli in the presence of 100, 300 or 500 ppm of Caesalpinia sappan extract ranged from 0.02 to 0.1 compared to 0.6 in the absence of Caesalpinia sappan extract, indicating that growth of E. coli was significantly inhibited within 24 hours by the addition of at least 300 ppm of Caesalpinia sappan extract. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 300 ppm of Rhus javanica extract ranged from 0.02 to 0.2 compared to 0.5 in the absence of Rhus javanica extract, indicating that growth of E. coli was also significantly inhibited within 24 hours by the addition of at least 500 ppm of Rhus javanica extract. In conclusion, these findings suggest that extracts from medicinal herbs may play important roles in antimicrobial activities against E. coli.

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Isolation and Identification of Antioxidative Components from Bark of Rhus javanica Linne (붉나무 수피로부터 항산화 물질의 분리 및 동정)

  • Oh, Ji-Young;Choi, Ung;Kim, Yong-Suk;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.35 no.4
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    • pp.726-732
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    • 2003
  • The crude extracts from Rhus javanica Linne showed comparatively strong antioxidative activity in test oils. Antioxidative components were isolated and identified by column chromatography, thin layer chromatography, UV, and NMR. These antioxidative components were added to several oils to compare antioxidative activity with several commercial antioxidants, such as BHA, BHT, and tocopherol. After the sixth column chromatography, one fraction (R-18-9-3-2-4-2) was separated from chloroform layer of Rhus javanica Linne. The R-18-9-3-2-4-2 fraction was identified as methyl gallate by $^1H-NMR$ and $^{13}C-NMR$ and confirmed with methyl gallate standard as authentic. The R18-9-3-2-4-2 fraction from chloroform layer of Rhus javanica Linne showed stronger activity than that of the ${\alpha}-,\;{\delta}-tocopherol$, BHT, and BHA at the same concentration.

Evaluation of the antioxidant potential and enzyme activities in species of Rhus (옻나무류의 항산화력 및 항산화 효소 활성)

  • 정형진;김은희;이건주;정규영;임종국;유정민;심영은;박재호
    • Korean Journal of Plant Resources
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    • v.14 no.3
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    • pp.220-228
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    • 2001
  • Comparison of the antioxidant potential and enzyme activities of the extracts of Rhus showed considerable differences. The antioxidative activities of Rhus javanica were the highest of three species tried (7 $\mu\textrm{g}$/$m\ell$). The highest activities showed in fraction No.3 of 12 fractions by Sephadex LH-20 column chromatography and the antioxidative activity showed, in purified extract of each stem,1.5 $\mu\textrm{g}$/$m\ell$ (in Rhus verniciflua), 1.9 $\mu\textrm{g}$/$m\ell$ (in Rhus javanica) and 2.3 $\mu\textrm{g}$/$m\ell$ (in Rhus tricocarpa) respectively. These were identificated as phenolic compounds which are well known antioxidant compounds such as 2-propenoic acid (Caffeic acid), Benzoic acid (Gallic acid), 7-hydroxy- 6methoxy-2H-1-Benzopyran-2-one (Scopoletin). POD activities of stem were higher than leaf. Especially, POD activity in stem of Rhus javanica was 193 times higher than leaf. Rhus tricocarpa, however, showed very low POD activity. SOD activities of stem were higher than that of leaf in Rhus javanica and Rhus verniciflua but in Rhus tricocarpa, the activity of leaf was 25 times higher than that of stem.

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The Removal of Pb by Plants (식물을 이용한 납(Pb) 제거)

  • Cho, Moon-Chul;Lee, Sang-Hwa;Park, Young-Seek;Suh, Kuen-Hack;Ahn, Kab-Hwan
    • Journal of Environmental Science International
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    • v.10 no.4
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    • pp.269-273
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    • 2001
  • Biosorption of Pb was evaluated for plants, Persicaria chinensis, Oenanthe javanica and Salvinia natnas. The adsorption equilibrium was reached in about 1hr for Pb and the highest adsorption capacity was 150mg Pb/g biomass at S.natans. Pb adsorption process showed a pseudo second order irreversible reaction. The highest initial adsorption rate was 2000mg pb/g biomass/hr at O.javanica. In spite of pH variation, Pb adsorption capacity by was selection, Pb was selectively adsorved. The selectivity of mixture solution showed the adsorption order of Pb>Cu>Cr>Cd. The Pb adsorption capacity of P. chinensis pretreated with NaOH was increased by 30% in comparison with that of no treatment.

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Isorhamnetin from Oenanthe javanica Attenuates Fibrosis in Rat Hepatic Stellate Cells via Inhibition of ERK Signaling Pathway

  • Lee, Mi-Kyeong;Yang, Hye-Kyung;Ha, Na-Ry;Sung, Sang-Hyun;Kim, Young-Choong
    • Natural Product Sciences
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    • v.14 no.2
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    • pp.81-85
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    • 2008
  • Isorhamnetin isolated from Oenanthe javanica significantly inhibited proliferation and collagen production in HSC-T6 cells in concentration- and time-dependent manners. Pretreatment of HSC-T6 cells with isorhamnetin significantly inhibited serum-induced ERK phosphorylation, in a similar manner as PD98059, a known MEK inhibitor. These results suggested that isorhamnetin reduced collagen production in HSC-T6 cells, in part, via inhibition of ERK signaling pathway.