Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Evaluation of the antioxidant potential and enzyme activities in species of Rhus

옻나무류의 항산화력 및 항산화 효소 활성

  • Published : 2001.10.01
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Abstract

Comparison of the antioxidant potential and enzyme activities of the extracts of Rhus showed considerable differences. The antioxidative activities of Rhus javanica were the highest of three species tried (7 $\mu\textrm{g}$/$m\ell$). The highest activities showed in fraction No.3 of 12 fractions by Sephadex LH-20 column chromatography and the antioxidative activity showed, in purified extract of each stem,1.5 $\mu\textrm{g}$/$m\ell$ (in Rhus verniciflua), 1.9 $\mu\textrm{g}$/$m\ell$ (in Rhus javanica) and 2.3 $\mu\textrm{g}$/$m\ell$ (in Rhus tricocarpa) respectively. These were identificated as phenolic compounds which are well known antioxidant compounds such as 2-propenoic acid (Caffeic acid), Benzoic acid (Gallic acid), 7-hydroxy- 6methoxy-2H-1-Benzopyran-2-one (Scopoletin). POD activities of stem were higher than leaf. Especially, POD activity in stem of Rhus javanica was 193 times higher than leaf. Rhus tricocarpa, however, showed very low POD activity. SOD activities of stem were higher than that of leaf in Rhus javanica and Rhus verniciflua but in Rhus tricocarpa, the activity of leaf was 25 times higher than that of stem.

한국에서 자라고 있는 옻나무속 3종의 항산화력을 조사하여 본 결과, 옻나무속 3종의 각 부위별 ethyl acetate 추출물에 대한 항산화능은 개옻나무의 경우 잎과 줄기에서 각각 32, 34 $\mu\textrm{g}$/$m\ell$, 옻나무의 경우 잎과 줄기에서 각각 39,21 $\mu\textrm{g}$/$m\ell$의 활성을 나타내었다. Sephadex LH-20 column chromatography를 이용하여 줄기 부위의 추출물을 12개의 fraction으로 분리하여 항산화능을 측정한 결과, fraction No.3이 가장 높은 항산화 활성을 나타내었으며, 옻나무가 1.5, 붉나무가 1.9, 개옻나무가 2.3 ug/$m\ell$ DPPH가를 나타내었다. 줄기 추출물로부터 2-propenoic acid (Caffeic acid), Benzoic acid (Gallic acid), 7-hydroxy-6- methoxy-2H-1-Benzopyran-2-one (Scopoletin) 등과 같은 phenol계 물질들이 동정되었다. 옻나무속 3종의 부위별 POD 활성 은 잎에 비해 줄기에서 193배 활성이 높게 나타났고 종간에는 붉나무가 0.58 unit/mg protein로 가장 높게 나타났으며 옻나무, 개옻나무 순으로 높게 나타났다. SOD 활성은 옻나무와 붉나무의 잎에 비해 줄기에서 높은 활성이 나타났으나,개옻나무는 잎이 줄기에 비해 25배 높게 나타났다.

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