• Title/Summary/Keyword: isolation systems

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Isolation and Characterization of Lethal Mutation near the unc-29 (LG I) Region of Caenorhabditis elegans

  • Lee, Jin-Sook;An, Joo-Hong
    • Animal cells and systems
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    • v.2 no.1
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    • pp.123-131
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    • 1998
  • The unc-29 region on the chromosome I of Caenorhabditis elegans has been mutagenized in order to obtain lethal mutations. In this screen, the uncoordinated phenotype of unc-29 (e193) mutant was used to identify any lethal mutations closely linked to the unc-29 gene, which encodes a subunit of nicotinic acetylcholine receptors. We have isolated six independent mutations (jh1 to jh6) out of approximately 5,200 ethyl methanesulfonate(EMS) treated haploids. Four of the six mutations demonstrated embryonic lethal phenotypes, while the other two showed embryonic and larval lethal phenotypes. Terminal phenotypes observed in two mutations (jh1 and jh2) indicated developmental defects specific to posterior part of embryos which appeared similar to the phenotypes observed in nob (no back end) mutants. Another mutation (jh4) resulted in an interesting phenotype of body-wall muscle degeneration at larval stage. These mutations were mapped by using three-factor crosses and deficiency mutants in this region. Here we report genetic analysis and characterization of these lethal mutations.

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Isolation and Characterization of the Ribosomal Protein 46 Gene in Drosophila melanogaster

    • Animal cells and systems
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    • v.2 no.1
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    • pp.113-116
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    • 1998
  • A cDNA clone coding for ribosomal protein 46 (rp46) which is a component of 60S ribosomal large subunit has been identified from Drosophila melanogaster. A cDNA clone encoding S. cerevisiae rp46 was used as a probe to screen a Drosophila larvae cDNA library. The DNA sequence analysis revealed that the cDNA coding for Drosophils rp46 contains a complete reading frame of 153 nucleotides coding for 51 amino acids. The deduced amino acid sequence showed 71-75% homology with those of other eukaryotic organisms. Northern blot analysis showed that about 1-kb rp46 transcripts are abundant throughout fly development. Whole mount embryonic mRNA in situ hybridization also showed no preferential distribution of the transcripts to any specific region. The chromosomal in situ hybridization revealed that the identified gene is localized at position 60C on the right arm of the second polytene chromosome with a possibility of single copy.

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Isolation and Characterization of Methyl Jasmonate -Inducible Genes in Chinese Cabbage

  • Park, Yong-Soon;Cho, Tae-Ju
    • Animal cells and systems
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    • v.7 no.4
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    • pp.337-343
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    • 2003
  • Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.

Design of a Fault Detector by using System Identification (시스템 식별 기법을 이용한 고장 탐지기 설계)

  • Park, Tae-Dong;Lee, Jea-Ho;Bai, Shan-Lin;Park, Ki-Heon
    • Proceedings of the KIEE Conference
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    • 2008.04a
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    • pp.199-200
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    • 2008
  • Demand for reliability and safety in modem systems has been increased in the research on fault detection and isolation. At traditional approaches to fault detection, redundant sensors have been used. More advanced methods are the residual analysis of signals which are created by the comparison between the actual plant behavior and the output response of a mathematical model. However, mathematical system models are difficult to obtain by using physical laws. These problems can be solved by system identification. In this paper, the transfer function of a direct current motor is estimated by using the system identification. And, the efficiency of the fault detector design is verified by using experiments.

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Design of the Large Diameter Faraday Rotator for High-power Laser Systems (고출력 레이저 시스템을 위한 대구경 Faraday Rotator 제작)

  • Hong, Sung-Ki;Seo, Young-Seok;Ko, Kwang-Hoon;Kim, Young-Won;Wee, Sang-Bong;Lim, Chang-Hwan
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.6
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    • pp.1026-1031
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    • 2008
  • We report on the design and experimental results of a large diameter faraday rotator for the high-power laser system(KLF: Kaeri laser facility) that was completed in late 2007s at Korea Atomic Energy Research Institute. The design involves modelling the magnetic field of cylindrical coil with large diameter(110 mm). Magnetic field generation coil is designed by 6 layers using a rectangular wire with cross-sectional area $3{\times}5[mm^2]$. We obtain an isolation ratio for optical feedback of 35 dB at 1064 nm and magnetic field strengths ${\sim}25kG$. We expect that the design can be widely used optical isolators in high-power laser system.

The characteristics of the luminous events caused between the ZnO arrester block and electrode (산화아연(ZnO) 피뢰기 소자와 전극사이에서 발생하는 방전광 특성)

  • Lee, B.H.;Pak, K.Y.;Kang, S.M.
    • Proceedings of the KIEE Conference
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    • 2004.07c
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    • pp.1869-1871
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    • 2004
  • The primary role of ZnO arresters is to protect transmission and distribution equipments against lightning surges. The extremely nonlinear V-I characteristics of the ZnO arrester obviates the need for isolation gaps and consequently it is continuously connected to line voltage. For this reason, ZnO arresters are degraded with increasing with time in actual power systems. In this work, the characteristics of the luminous events between the ZnO block and electrodes were investigated. As a result, the luminous events were effected by the impulse and the near polarity of the luminous event was intense near the grounded electrode. Also the luminous event may cause the degradation of ZnO arrester block.

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Maximum Slab Length of Floating Slab Track (플로팅 슬래브 궤도의 최대길이)

  • Huan, Nguyen Ha;Jang, Seung-Yup;Chung, Won-Seok
    • Proceedings of the KSR Conference
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    • 2011.05a
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    • pp.173-180
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    • 2011
  • Recently, many railway stations are built under the railway line in urban area. The passage of railway vehicles generates mechanical vibrations of a wide range of frequency. Thus, it is required to place structural vibration isolation systems to reduce vibration and noise originating from surrounding environments. This study is to investigate the maximum floating slab length based on track/floating slab interaction analyses. Actions to be taken into account include temperature, braking/acceleration, bending of the deck, and creep/shrinkage. The additional rail stress has been chosen for the criterion for the maximum slab length. In addition, further analyses are performed to include the stopper which restrict the in-plane movement of the floating slab track. Several alternatives for stopper positions were thoroughly studied in this study.

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FDI observer design for linear system via STWS

  • Ahn, Pius;Kim, Min-Hyung;Kim, Jae-Il;Lee, Moon-Hee;Ahn, Doo-Soo
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1423-1427
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    • 1997
  • This paper deals with an algebraic approach to FDI observer design procedure. In general, FDI observer can be designed a sLuenbrger-type and equations for unknown input and actuator fault estimation include derivation of system outputs which is not available from the measurement directly. At this point, this paper presents STWS approach which can convert the derivation procedure to the recursive algebraic form by using its orthogonality and disjointess to alleviate such problems.

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ART2 Neural Network Applications for Diagnosis of Sensor Fault in the Indoor Gas Monitoring System

  • Lee, In-Soo;Cho, Jung-Hwan;Shim, Chang-Hyun;Lee, Duk-Dong;Jeon, Gi-Joon
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.1727-1731
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    • 2004
  • We propose an ART2 neural network-based fault diagnosis method to diagnose of sensor in the gas monitoring system. In the proposed method, using thermal modulation of operating temperature of sensor, the signal patterns are extracted from the voltage of load resistance. Also, fault classifier by ART2 NN (adaptive resonance theory 2 neural network) with uneven vigilance parameters is used for fault isolation. The performances of the proposed fault diagnosis method are shown by simulation results using real data obtained from the gas monitoring system.

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Unscented Kalman Filter For Aircraft Sensor Fault Detection

  • Kim, In-Jung;Kim, You-Dan
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2335-2339
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    • 2003
  • To prevent the critical situation due to the fault in the aircraft sensor system, the fault tolerant system with triple or quadruple redundancy can be made. However, if the faults are occurred in two or more than sensors simultaneously, the conventional fault detection process, such as cross-channel monitoring, may give the wrong fault alarm. For this case, we can detect the fault by estimating the state vector based on the system dynamics model, which is nonlinear for aircraft. In this paper, we propose the unscented Kalman filter to estimate the nonlinear state vector. This filter utilizes the so-called unscented transformation of sigma points featured the statistical characteristics of the random variable. For verification, we perform the simulations for F-16 aircraft with accelerometers, gyros, GPS and air data system.

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