• The Journal of Internal Korean Medicine
• /
• v.37 no.1
• /
• pp.65-76
• /
• 2016

Objectives: The purpose of this study was to identify the inhibitory effects of Hwangheuk-san (HHS), a Korean multi-herb formula comprising four medicinal herbs, on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, using the human bladder cancer 5637 cell line.Methods: Cell viability, motility, and invasion were assessed by 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphnyl-2H-tetrazolium bromide (MTT), wound healing migration, and Transwell assays, respectively. Gene expression was detected by Western blot analysis. In addition, the activities of matrix metalloproteinases (MMPs) and the values for transepithelial electrical resistance (TER) were analyzed using a Gelatinase Activity Assay Kit and an Epithelial Tissue Voltohmmeter, respectively.Results: Our data indicated that within the concentration range that was not cytotoxic, HHS effectively inhibited the cell motility and invasiveness of 5637 cells. HHS markedly decreased the expression and activity of MMP-2 and MMP-9, which was associated with unregulation of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2. Further investigation revealed that phosphorylation of phosphatidylinositol 3-kinase (PI3K) and AKT was decreased in HHS-treated 5637 cells, and a PI3K/AKT inhibitor synergistically reduced the inhibition of migration and invasion and also inactivated MMP-2 and MMP-9. Moreover, HHS increased the tightening of tight junctions (TJs), which was demonstrated by an increase in the TER, and reduced the expression the levels of claudin family members (claudin-3 and -4), which are major components involved in the tightening of TJs.Conclusions: The present findings demonstrated that HHS attenuated the migration and invasion of bladder cancer 5637 cells by modulating the activity of the PI3K/Akt signaling pathway and also through TJ tightening.

• Journal of Gastric Cancer
• /
• v.18 no.1
• /
• pp.37-47
• /
• 2018

Purpose: The endoscopic management of a fully covered self-expandable metal stent (SEMS) has been suggested for the primary treatment of patients with anastomotic leaks after total gastrectomy. Embedded stents due to tissue ingrowth and migration are the main obstacles in endoscopic stent management. Materials and Methods: The effectiveness and safety of endoscopic management were evaluated for anastomotic leaks when using a benign fully covered SEMS with an anchoring thread and thick silicone covering the membrane to prevent stent embedding and migration. We retrospectively reviewed the data of 14 consecutive patients with gastric cancer and anastomotic leaks after total gastrectomy treated from January 2009 to December 2016. Results: The technical success rate of endoscopic stent replacement was 100%, and the rate of complete leaks closure was 85.7% (n=12). The mean size of leaks was 13.1 mm (range, 3-30 mm). The time interval from operation to stent replacement was 10.7 days (range, 3-35 days) and the interval from stent replacement to extraction was 32.3 days (range, 18-49 days). The complication rate was 14.1%, and included a single jejunal ulcer and delayed stricture at the site of leakage. No embedded stent or migration occurred. Two patients died due to progression of pneumonia and septic shock 2 weeks after stent replacement. Conclusions: A benign fully covered SEMS with an anchoring thread and thick membrane is an effective and safe stent in patients with anastomotic leaks after total gastrectomy. The novelty of this stent is that it provides complete prevention of stent migration and embedding, compared with conventional fully covered SEMS.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.59 no.5
• /
• pp.1-15
• /
• 2017

In this study, we visualized the regional migration in Korea from 2001 to 2015 using the Chord diagram which can represents amount of migration and flows at the same time. In addition, we constructed a migration network and analyzed the degree centrality of each region for identifying the main regions linking to various regions. In 2001~2005, most of population moved into Geonggi from various regions. However, the capital function was transferred to Sejong in 2011~2015, and population moving into Sejong and Chungnam was increased significantly. The main outflow of population in migration network were shown at the regions in Jeonbuk and Gyeongbuk province in 2001~2004, and recently the regions in Gyeongnam, Gyeonggi, and Seoul were identified as the main nodes in terms of outflow of population. We also focused on migration in rural area through degree centrality, and cord diagram in Chungnam, Jeonbuk, and Jeonnam where include the representative crop area. In 2015. there was the significant increase of migration from Gyeonggi to Chungnam, and internal migration within Jeonbuk increased rather than cross-border migration. In addition, migration from Jeonam to capital area decreased in 2015 but migration among cities within Jeonman increased. In particular, Yesan-gun showed the significant migration to other cities in Jeonnam. Population is necessary to develop community and sustain economic growth in rural regions. Therefore, migration is important for the transfer of manpower. The strength of this study is to approach the temporal change of migration from the viewpoint on quantitative and structural characteristics.

• The Journal of Churna Manual Medicine for Spine and Nerves
• /
• v.2 no.2
• /
• pp.49-56
• /
• 2007

Objectives : This study is to investigate the clinical application of conservative treatment to a patient who has the paresthesia and migration on L-spine MRI. Methods : A male patient, 34 years old, who has paresthesia and migration on L-spine MRI was evaluated after conservative oriental medical and chuna treatment by Visual Analog Scale(VAS), Numeric Rating Scale(NRS), Straight Leg Rasing Test(SLRT), Sensation, MRI follow-up. Results : 1. Visual Analog Scale(VAS), Numeric Rating Scale(NRS), Straight Leg Rasing Test(SLRT), Sense was significantly improved after conservative oriental medical and chuna treatment. 2. Also it was observed that herniated disc was decreased by L-spine MRI follow-up. Conclusions : Conservative oriental medical and chuna treatment can be effective for decreasing pain and improving paresthesia, also decreasing the volume of herniated disc.

• Journal of the Korean Geotechnical Society
• /
• v.34 no.11
• /
• pp.33-41
• /
• 2018

Soil internal erosion is a phenomenon in which fines attached to the solid skeleton are detached by fluid flow, and this continuous fine migration weakens the hydro-mechanical characteristics of the ground structure. This paper proposed governing equations for fine migration in pore spaces and its related scheme for the numerical analysis. Phase diagram for fine particles includes three different states: detached fines in the liquid phase ($c_e$), attached fines in the solid phase (${\sigma}_a$), and pore-clogged fines in the solid phase (${\sigma}_s$). Numerical formulations for finite element method are developed based on the hydraulic governing equations of pore fluid and fine migration. This study proposed a method of estimating model parameters for fine detachment, attachment, and clogging from 1D erosion experiments. And it proposed an analytical formula for hydraulic permeability function considering fine clogging. Numerical analysis of the previous erosion test developed the numerical scheme and verified the adequacy of fine migration models.

• Asian Pacific Journal of Cancer Prevention
• /
• v.18 no.11
• /
• pp.2919-2923
• /
• 2017

Objective: Anthocyanins belong to a class of flavonoids, exhibiting antioxidant and anti-inflammatory actions have been reported to have anti-cancer effects. Here, we investigated whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in human lung cancer A549 cells, which are critically involved in cancer metastasis. Methods: We used anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) which has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. We have performed cell proliferation assays, cell invasion assay, gelatin zymography, wound healing assay and western blotting to examine whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in A549 cells. Result: AIMs did not inhibit cancer cell proliferation on A549 cells. Also, AIMs suppressed cancer migration, and invasion by supressing MMP-2 and MMP-9 expression. The Immuno-blotting results also revealed that AIMs suppressed the proteins involved in cancer proliferation (COX-2, C-myc, cyclin D1), migration and invasion (MMP-2, MMP-9), anti-apoptosis (XIAP, and c-IAP2), adhesion and angiogenesis (ICAM-1, VEGF). Conclusion: This study demonstrates that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit cancer proliferation, cancer migration, and invasion that is involve in cancer-metastasis. This study provides evidence that AIMs might have anti-cancer effects on human lung cancer.

• Journal of the Korean Chemical Society
• /
• v.23 no.1
• /
• pp.20-29
• /
• 1979

The origin of barriers to internal rotation of hydrogen peroxide and ethane is investigated by using the concept of Bonding and Antibonding Regions. The strong bond formations between the axial and end atoms on the same side make the real charge densities in these molecules less dependent on conformations than those in the hypothetical molecules having no axial atoms. Thus, the existence of the axial atoms should induce the migration of the transition density from the Bonding region to the Antibonding region. Barrier to internal rotation can be understood in terms of this migration of the transition density to such an extent that the change in nuclear-nuclear repulsion energy becomes the dominating part of the total perturbation energy.

• The Bulletin of The Korean Astronomical Society
• /
• v.44 no.1
• /
• pp.47.1-47.1
• /
• 2019

A gas giant formed in a massive protoplanetary disk via gravitational instability migrates inward due to its gravitational interaction with the disk. Planet migration occurs in various ways depending on the disk structure and internal processes, but previous studies only considered quantitative radiative feedback resulting from mass accretion onto the planet. Allowing for accretion feedback, we perform three-dimensional hydrodynamic simulations with GIZMO to investigate orbital evolution of giant planets in a protoplanetary subject to -cooling. This work shows a planet gains mass due to accretion and gradually opens a gap as it moves inward. The migration in the end halts when the planet clears the gas around its orbit. A more massive planet grows its mass faster and migrates more rapidly, stalling at an orbit farther away from the protostar. Models with a cold disk readily construct a circumplanetary disk and result in high mass growth of the planet. Accretion feedback, in general, reduces the rate of the planet growth and delays migration. We discuss our results with GIZMO in comparison with the previous results with different codes.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.48 no.3
• /
• pp.5-13
• /
• 2016

This study investigated the effects of different factors on the migration of a fluorescent whitening agent (FWA) from paper treated with FWAs to non-fluorescent papers. FWA migration experiments were carried out in vertical and friction contacts between the papers dyed with FWAs and non-fluorescent papers. During the experiments, we identified the effects of the addition and types of FWAs, contact time, temperature, and relative humidity (RH) on FWA migration. The fluorescence indices of the non-fluorescent papers were measured before and after the migration experiments, and the Student's t test, a statistical tool, was utilized to compare results from different migration experiments. In vertical contact experiments, FWA migration to non-fluorescent paper was observed at $30^{\circ}C$ and 70% RH; this was attributed to the high moisture content of the paper. FWA migration did not occur significantly at $23^{\circ}C$ and 50% RH. In the friction contact experiments, FWA migrations were identified at both temperature conditions and RH percentages. The addition and types of FWAs did not increase the fluorescence index of non-fluorescent papers. Therefore, it was concluded that the moisture content of paper and the friction contact affected FWA migration from the papers containing internal and surface FWAs.

• Bulletin of the Korean Chemical Society
• /
• v.23 no.1
• /
• pp.9-10
• /
• 2002