• Parasites, Hosts and Diseases
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• v.56 no.5
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• pp.419-427
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• 2018

This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes of traveler's diarrhea. Three target genes were specifically and simultaneously detected by the TaqMan probe method for multiple parasitic infection cases, including Cryptosporidium oocyst wall protein for C. parvum, glutamate dehydrogenase for G. lamblia, and internal transcribed spacer 1 for C. cayetanensis. Gene product 21 for bacteriophage T4 was used as an internal control DNA target for monitoring human stool DNA amplification. TaqMan probes were prepared using 4 fluorescent dyes, $FAM^{TM}$, $HEX^{TM}$, $Cy5^{TM}$, and CAL Fluor $Red^{(R)}$ 610 on C. parvum, G. lamblia, C. cayetanensis, and bacteriophage T4, respectively. We developed a novel primer-probe set for each parasite, a primer-probe cocktail (a mixture of primers and probes for the parasites and the internal control) for multiplex real-time PCR analysis, and a protocol for this detection method. Multiplex real-time PCR with the primer-probe cocktail successfully and specifically detected the target genes of C. parvum, G. lamblia, and C. cayetanensis in the mixed spiked human stool sample. The limit of detection for our assay was $2{\times}10$ copies for C. parvum and for C. cayetanensis, while it was $2{\times}10^3$ copies for G. lamblia. We propose that the multiplex real-time PCR detection method developed here is a useful method for simultaneously diagnosing the most common causative protozoa in traveler's diarrhea.

• Proceedings of the KIEE Conference
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• 2006.10c
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• pp.285-287
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• 2006

The paper treats the nonlinear robust control of Chua's circuit having Chuar's diode as an element based on the internal model principle. The Chua's diode has unknown nonlinear parameters and the circuits parameters are alos assumend unknown. Nonlinear regulator equations are established to obtain 3-fold equilibrium equations on which the output error is zero. Also an internal model of the 3-fold exosystem is constructed for obtaining the control law. Pole Placement method is used for obtaining the feeback control law. Simulation results are presented for tracking the sinusoidal and constant reference input signal. Asymptotic trajectory control and the suppression of chaotic motion in spite of uncertainties in the system are accomplished.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.10
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• pp.1501-1513
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• 2001

Disturbance observer, adaptive robust control, and enhanced internal model control are model based disturbance attenuation methods famous for robust motion controller which can satisfy desired performance and robustness of high-speed/high-accuracy positioning systems. In this paper, these are shown to be the same scheme with different parameterizations. To do this, a generalized framework, called as RIC(robust internal-loop compensator) is proposed and the conventional schemes are analyzed in the RIC framework. Through this analysis, it can be shown that there are inherent similarities between the schemes and advantages of the RIC in the viewpoint of controller design. This is verified through simulations and experiments.

• The Journal of Fisheries Business Administration
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• v.22 no.2
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• pp.101-148
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• 1991

The fisheries cooperative (FC) performs the economic and nonprofitable activity to get the fundamental objective of enhancing cooperative members' economic and social position. The internal control system fitted for a local FC should be required for not only solving the resulting problem from the complexity of FC environment nowaday but also delegating authorities and performance from FC Federation to a local FC by implementing the local autonomy. The methodology of this study is to empirically test and to analyze the condition of FC internal control system (FCICS) by the questionnaire survey. The actual condition of FCICS in Korea is analyzed by the questionnaire and the detailed contents are as follows : (1) sending 208 questionnaire consisting of 162 questions, and receiving 92 replies from 39 manufactures (business firms) and 15 banks in Gyungnam and Pusan area and 25 FC and 13 agricultural cooperative (AC) in Korea, (2) the analyzed results of FC and AC are treated simultaneously. In the fundamentals of above analyzed results, the evaluation model of FCICS is tried to construct from the relationship between the financial condition of FC and the internal control elements through the stepwise regression method. (1) By the stepwise regression method, the number of FC officials $(X_1)$, the experimental number of regular auditing $(X_7)$, and auditing duty years $(X_8)$ are finally accepted as independent variables, (2) and the final model becomes $Y=-1.53526+0.34455X_1+0.24513X_7+0.16585X_8$/ and this model explains to the extent of 47.826%. From the above study, following proposals are to be suggested: (1) The function and problem of internal control in FCICS is able to be improved by enforcing the function of FCICS and enriching the management's recognition of FCICS (2) The cooperative president can bring up good FC by the rational operation of FCICS according to the size and the performance pattern of FC, adding up to enhance members' economic and social position.

• The Korean Journal of Nuclear Medicine
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• v.23 no.2
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• pp.225-230
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• 1989

According to the development of monoclonal antibodies against cyclosporine, it became available to replace the conventional polyclonal antibody method with new monoclonal antibody method to measure the blood level of cyclosporine by radioimmunoassay. We compared the results obtained by the two methods: polyclonal antibody and monoclonal antibody method. The results were obtained as follows: 1) We obtained mean value 137.3 ng/ml and CV 16.1% from plasma sample, and mean values 495.7 ng/ml and 1053.8 ng/ml and CVs 19.3% and 17.4% respectively from whole blood sample by polyclonal antibody method. 2) For the two control groups, 100 ng/ml 400 ng/ml each, we obtained that the CVs were 20.2% and 14.0% respectively from plasma sample, and 11 9% and 13.1% respectively from whole blood sample by monoclonal antibody method. In conclusion, we found that cyclosporine RIA was a relatively reliable method to measure blood or plasma concentration. Especially RIA using monocloanl antibody showed less degree of error in measurement compared to polyclonal antibody method.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.7 no.3
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• pp.118-125
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• 1998

Chip control is a major problem in automatic machining process, especially in finish turning operation. In this case, chip breaker is one of the important factors to be determined. As unbroken chips are grown. these deteriorate the surface roughness. and proces automation can not be carried out. In this study to get rid of chip curling problem while turning internal hole. optimal chip breaker is selected from the experiment. The experiment is planned with Taguchi's method that is based on the orthogonal arrary of design factors. From the response table. cutting speed, feedrate, depth of cut and tool geometry turn to be major factors affecting chip formation. Then, optimal chip breaker is selected. and this is verified as good enough for chip control from the experiment.

• Korean Business Review
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• v.18 no.2
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• pp.45-60
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• 2005

CSA(Control Self Assessment) is a new internal control technique whose objective is to control the human resource performance, the manager's philosophy, the business ethics, the effective communication and decision making etc. The purpose of this study is to find out if the CSA improve the quality of internal control. With the questionnaire method the following conclusions are reached. 1) Level of the management supports influence the level of the workshop participation. 2) Anonymity during the workshop session influences the level of workshop participation. 3) Level of the organizational committment influences the level of the participation 4) The level of workshop performance significantly influences the level of internal contro improvement.

• Journal of Families and Better Life
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• v.19 no.6
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• pp.185-196
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• 2001

The purpose of this study was to examine the relationships among the internal locus of control, perceived family support, family cohesion, family adaptability, and children's social behaviors. The subjects were 285 6th and 8th grade students and their homeroom teachers. A questionnaire was used as survey method. Data were analyzed by Pearson's correlation, t-test, ANOVA, Duncan post test. and multiple regression. The results are as follows: First, internal locus of control was positively related to social competence and negatively related to antisocial behavior. Perceived family support, family cohesion and adaptability were positively related to social competence and negatively related to antisocial behavior. Second, girls were higher internal locus of control, family cohesion, family adaptability and social competence and lower in antisocial behavior than boys. The 8th graders had lower scores than 6th graders in perceived family support, family cohesion, family adaptability and social competence and higher level of antisocial behavior. Third, perceived family support and family adaptability impacted social competence in girls, and grade impacted social competence in boys. Grade, perceived family support and locus of control had significant effects on the antisocial behavior in boys, and perceived family support impacted antisocial behavior in girls.

• Korean Journal of Adult Nursing
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• v.27 no.3
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• pp.294-303
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• 2015

Purpose: The purpose of this study was to determine the relationships among health locus of control, resilience, social support, and health promoting behavior in patients with coronary artery diseases. Methods: This study utilized a descriptive correlational design using a survey method. The subjects were 165 coronary artery disease patients at the cardiology out-patient clinic of the C university hospital. Data were collected through Health Locus of Control Scale, Resilience Scale, ENRICHD Social Support Instrument, Health Promoting Lifestyle Profile scale. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient and hierarchial regression. Results: There were significant positive bivariate correlations of health promoting behavior with internal health locus of control, doctors health locus of control, resilience and social support respectively, and correlation between resilience and social support. Among predictors, internal health locus of control (${\beta}$=.28), social support (${\beta}$=.28) and resilience (${\beta}$=.25) had statistically significant influences on health promoting behavior. Conclusion: These results proposed that internal health locus of control, social support and resilience have important influences on health promoting behavior. Nursing interventions to enhance internal health locus of control, resilience and social support might be essential for patients with coronary artery disease in order to promote their health behaviors.

• The Journal of Internal Korean Medicine
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• v.28 no.4
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• pp.779-790
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• 2007

Objective : Although chronic non-bacterial prostatitis is a common disease, it is very difficult to treat effectively. GLS (Gleditsiae Spina) has traditionally been used in treatment of urinary tract inflammation and voiding disturbance. In this study, we investigated the therapeutic effects and action mechanism of GLS in the rat model of non-bacterial prostatitis induced by castration and testosterone treatment. Method : We observed four experimental objects of normal group, control group, testosterone group, and GLS group. Rats were treated with 17$\beta$-estradiol after castration for induction of experimental non-bacteral prostatitis, which is similar to human chronic prostatitis in histophatological profiles. GLS and testosterone were administered as an experimental specimen and a positive control, respectively. The prostates were evaluated by histopahological parameters including the epithelial score and epithelio-stromal ratio for glandular damage. Also, the prostates were observed by hematological alterations of WBC, RBC, hemoglobin, hematocrit and platelet. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with GLS showed a diminished range of tissue damage. Epithelial score was improved in GLS over that of the control. The epithelial-stromal ratio was lower in GLS when compared to that of the control. Conclusion : These findings suggest that GLS may protect the glandular epithelial cells. We concluded that GLS could be a useful remedy agent for treating chronic non-bacterial prostatitis.