• 대한약침학회지
• /
• 제14권3호
• /
• pp.5-17
• /
• 2011

Objectives : Gliosis becomes physical and mechanical barrier to axonal regeneration. Reactive gliosis induced by middle cerebral artery occlusion is involved with up-regulation of CD81 and GFAP (Glial fibrillary acidic protein). The current study is to examine the effect of the Angelica gigas Nakai(intravenous injection. 100 mg/kg twice in a day) on CD81 and GFAP of the rat in the brain after middle cerebral artery occlusion. Methods : Cerebral infarction was induced by middle cerebral artery occlusion. And after intravenous injection of water extract of Angelica gigas Nakai, the size of cerebral infarction was measured. Examination of optical microscope were also used to detect the expression of CD81 and GFAP in the brain of the rat. Results : The following results were obtained : We found that size of cerebral infarcion induced by MCAO (Middle Cerebral Artery Occlusion) in rats were decreased after intravenous injection of Angelica gigas Nakai. We injected the extract of Angelica gigas Nakai to the MCAO in rats, and the optical microscope study showed that Angelica gigas Nakai had effect on protecting the cells of hippocampus. We found that GFAP, CD81 and ERK of the brain in rats with cerebral infarction after MCAO were meaningfully decreased after intravenous injecting Angelica gigas Nakai. We found that c-Fos expression of the brain in rats with cerebral infarction after MCAO were significantly increased after intravenous injecting Angelica gigas Nakai. Conclusions : These results indicate that Angelica gigas Nakai could suppress the reactive gliosis, which disturbs the astrocyte regeneration in the brain of the rat with cerebral infarction after MCAO by controlling the expression of CD81 and GFAP. And the effect may be modulated by the up-regulation of c-Fos and ERK.

• 대한영양사협회학술지
• /
• 제11권2호
• /
• pp.137-146
• /
• 2005

This study was carried out to investigate the relationship between diabetic control and related factors of the practice of diet therapy which affects mostly diabetic patients’ dietary compliance. A questionnaire survey was conducted on 128 diabetic patients who had visited Internal medicine endocrinology clinic at University Medical Center of Daegu area. The questionnaires including clinical characteristics, meal regularity and food intake which shows dietary compliance, intrinsic barriers to the practice of diet therapy and knowledge of diet therapy were asked and analyzed. The factor which affects HbA1c was intrinsic barriers and HbA1c became higher as the level of intrinsic barriers was increasing. The education on diet therapy had no influence on the intrinsic barriers, but the level of knowledge on diet therapy was shown higher in the educated patients. The above results suggest that the practice of diet therapy should be leaded to develop behavioral aspects through resonable motivation on dietary compliance along with removing intrinsic barriers rather than simply providing information.

• IMMUNE NETWORK
• /
• 제15권4호
• /
• pp.206-211
• /
• 2015

Pulmonary edema is a major cause of mortality due to acute lung injury (ALI). The involvement of protein kinase C-${\delta}$ (PKC-${\delta}$) in ALI has been a controversial topic. Here we investigated PKC-${\delta}$ function in ALI using PKC-${\delta}$ knockout (KO) mice and PKC inhibitors. Our results indicated that although the ability to produce proinflammatory mediators in response to LPS injury in PKC-${\delta}$ KO mice was similar to that of control mice, they showed enhanced recruitment of neutrophils to the lung and more severe pulmonary edema. PKC-${\delta}$ inhibition promoted barrier dysfunction in an endothelial cell layer in vitro, and administration of a PKC-${\delta}$-specific inhibitor significantly increased steady state vascular permeability. A neutrophil transmigration assay indicated that the PKC-${\delta}$ inhibition increased neutrophil transmigration through an endothelial monolayer. This suggests that PKC-${\delta}$ inhibition induces structural changes in endothelial cells, allowing extravasation of proteins and neutrophils.

• 한국전산유체공학회지
• /
• 제16권4호
• /
• pp.92-99
• /
• 2011

In order to protect the turbine blade from working fluid of high temperature, many cooling techniques such as internal convection cooling, film cooling, impinging jet cooling and thermal barrier coating have been developed. With all other things, film-cooling has been widely used as the important alternative. In the present work, numerical analysis has been performed to investigate and to compare the film-cooling performance of various film-cooling hole schemes such as cylindrical, crescent, louver, and dumbbell holes. To analyze the turbulent flow and the film-cooling mechanism, three-dimensional Reynolds-averaged Navier-Stokes analysis has been performed with shear stress transport turbulence model. The validation of numerical results has been assessed in comparison with experimental data. The characteristics of fluid flow and the film-cooling performance for each shaped hole have been investigated and evaluated in terms of centerline, laterally averaged and spatially averaged film-cooling effectivenesses. Among the film cooling holes, the dumbbell shaped hole shows better film-cooling effectiveness than the other shaped holes. And the louver and cylindrical shaped hole show the worst film cooling performance, and concentrated flows on near the centerline only.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권2호
• /
• pp.360-372
• /
• 2020

Objective: Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target mutagenesis caused by these nucleases is a major barrier to putative clinical applications. Furthermore, off-target mutagenesis by genome editing has not yet been addressed in pigs. Methods: Here, we generated genetically inheritable α-1,3-galactosyltransferase (GGTA1) knockout Yucatan miniature pigs by combining transcription activator-like effector nuclease (TALEN) and nuclear transfer. For precise estimation of genomic mutations induced by TALEN in GGTA1 knockout pigs, we obtained the whole-genome sequence of the donor cells for use as an internal control genome. Results: In-depth whole-genome sequencing analysis demonstrated that TALEN-mediated GGTA1 knockout pigs had a comparable mutation rate to homologous recombination-treated pigs and wild-type strain controls. RNA sequencing analysis associated with genomic mutations revealed that TALEN-induced off-target mutations had no discernable effect on RNA transcript abundance. Conclusion: Therefore, TALEN appears to be a precise and safe tool for generating genomeedited pigs, and the TALEN-mediated GGTA1 knockout Yucatan miniature pigs produced in this study can serve as a safe and effective organ and tissue resource for clinical applications.

• 농촌계획
• /
• 제21권2호
• /
• pp.51-61
• /
• 2015

The necessity of universal design for forestry is increasing. Although several studies applied universal design in forest site, they had been only concentrated on the barrier-free design for the limited activities rather than overall utilization for considering all visitors from the disabled, the elderly and the infirm to the baby and child. The purpose of this study is to find out the principle of the universal design within forest welfare service facilities by analysing how socially disadvantaged people perceive overall usage of natural recreation forest area. This study figures out the main principles of universal design in forest welfare through analyzing usage of children and disabilities in Yumyeongsan natural recreation forest where is one of the popular type of forest welfare area. By doing focus interviews targeted on children and disabilities, the results are analyzed through semantic network analysis, objectively. The result proves that universal design in forest welfare area contains four principles; convenience, safety, diversity and amenity. Also it confirmed that disabilities need better internal space facilities and priority care. Through those characters of universal design, forest area will be able to cover more various type of users as forest welfare area.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2001년도 추계학술대회논문집B
• /
• pp.30-35
• /
• 2001

To get higher efficiency of gas turbine, The designer should have more higher turbine inlet temperature (TIT). Today, modem gas turbine having sophisticated cooling scheme has TIT above $1,700^{\circ}C$. In the korea, many gas turbine having TIT above $1,300^{\circ}C$ was imported and being operated, but the gas with high TIT above $1,300^{\circ}C$ in the turbine will give damage to liner of combustor, and blade of turbine and etc. So frequently maintenance for parts enduring high temperature was performed. In this study, the heat transfer analysis of cooling air in the internal cooling channel (network analysis) and temperature analysis of the blade (Finite Element Analysis) in the first stage rotor was conducted for development of the optimal cooling passage design procedure. The results of network analysis and FEM analysis of blade show that the high temperature spot are occured at the leading edge, trailing edge near tip, and platform. so to get more reliable performance of gas turbine, the more efficient cooling method should be applied at the leading edge and tip section. and the thermal barrier coating on the blade surface has important role in cooling blade.

• 생태와환경
• /
• 제49권2호
• /
• pp.124-129
• /
• 2016

본 연구는 하수처리장에서 최종 방류되는 하수처리수의 거품발생 원인분석과 저감방안을 제시하고자 수행되었다. 감조하천의 방류구에서 거품이 발생하는 것은 구조적인 문제점으로 파악되었다. 거품은 낙차에 의한 공기 연행과 확산방지막에 의한 내부축적이 주요한 원인이었다. 이러한 여건을 고려하여, 방류수로와 방류구 종단에서 미세스크린과 수중방류를 통해 거품발생을 완화시킬 수 있는 효과적인 방안을 제시하였다.

• Journal of Hospice and Palliative Care
• /
• 제11권2호
• /
• pp.106-110
• /
• 2008

완화적 진정은 임종이 임박한 불응성 증상을 가진 환자들에게 고려될 수 있는 치료이지만 부적절한 진정으로 인한 윤리적인 문제가 쟁점이 된다. 본 증례는 수차례항암화학요법과 완화적 방사선 치료에도 불구하고 진행되는 다발성 뼈 전이를 동반한 전이성 위암을 가진 50세 남자에게 완화적 진정을 시행했던 예를 권고안에 비추어 후향적 검토하였다. 환자는 표준적인 통증경감치료에 불응성으로 판단되었고 환자의 의사결정능력 부족으로 환자보호자에게 완화적 진정에 대한 치료 목적 및 예견되는 부작용을 설명한 후 midazolam으로 완화적 진정을 시작하였다. 본 증례에 통하여 완화적 진정의 적응, 완화적 진정의 시작 전 의사 결정, 완화적 진정 시작, 완화적 진정 시작 후의 돌봄에 대한 토의를 하였고 국내실정을 반영한 권고안 마련을 제의하는 바이다.

• 대한의생명과학회지
• /
• 제11권2호
• /
• pp.221-225
• /
• 2005

To investigate the spectroscopic findings of embolized lesions induced with a fat-emulsion technique with magnetic resonance images (MRI). A fat emulsion was made with 0.1 ml of triolein and 20 ml of normal saline. In 12 cats, the internal carotid artery was infused with the fat emulsion. Contrast-enhanced T1-weighted MRI and multivoxel spectroscopy were obtained at 1 hour, 1 and 4 days, and 1 week after embolization. NAA (N-acetylaspartate), Cr (creatine) and Cho (choline) were evaluated on the spectroscopy. Statitistical analysis wsperformed at the embolized and contralateral normal hemisphere in the integral and amplitude of NAA, Cr and Nho in time course. Also NAA/Cr and Cho/Cr were compared in both hemisphere and in time course. The emboli zed lesions showed contrast enhance ments on Gd-enhanced Tl-weighted i~ages at 1 hour. This contrast enhancement was decreased at day 1, and id not appear agter day 4. In spectroscopy, the embolized hemisphere showed no statistical difference to the normal contralateral side at 1 hour and in time course. NAA/Cr and Cho/Cr were not significantly different in both hemispheres at 1 hour and in time course. Cerebral-fat embolism induced by a triolein emulsion in cats revealed no statistical difference on MR spectroscopy. Triolein-emulsion can be used in the study of blood-brain barrier.