• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.613-619
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• 2003

The polyketide backbone of rifamycin B is assembled by the type I rifamycin polyketide synthase (PKS) encoded by the rifA-rifE genes. In order to produce novel analogs of rifamycin via engineering of the PKS genes, inactivation of the ${\beta}-ketoacyl:acyl$ carrier protein reductase (KR) domain in module 8 of rifD, by site-specific mutagenesis of the NADPH binding site, was attempted. Module 8 contains a nonfunctional dehydratase (DH) domain and a functional KR domain that is involved in the reduction of the ${\beta}-carbonyl$ group, resulting in the C-21 hydroxyl of rifamycin B. This mutant strain produced linear polyketides, from tetraketide to octaketide, which were also produced by a rifD-disruption mutant as a consequence of premature termination of the polyketide assembly. Another attempt to replace the DH domain of module 7, which has been considered nonfunctional, with a functional homolog derived from module 7 of rapamycin-producing PKS also resulted in the production of linear polyketides, including the heptaketide intermediate and its precursors. Premature release of the carbon chain assembly intermediates is an unusual property of the rifamycin PKS. that is not seen in other PKSs such as the erythromycin PKS.

• Journal of the Society of Disaster Information
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• v.16 no.1
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• pp.111-122
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• 2020

Purpose: In order to manage the steep slopes effectively, this study diagnose problems in the procedure of steep slopes management and propose Improved frame work is intended to mitigate human and property damage Method: Problems in the system are drawn through review of procedures for designation of collapse risk zones and fied investigation, interviews with local governments, and expert advice. Result: The selection stage, the subject of the management, the management method, and the factors that need to be improved by the management stage before the evaluation are derived. Conclusion: This paper identified the problems raised and drew improvements and presented the research direction for the development of the new system (plan) and the steep slope site.

• Proceedings of the Technology Innovation Conference
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• 1996.12a
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• pp.196-238
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• 1996

Because global innovation-based competition is increasing and the amount of R&D expenditures becomes severely large, it is more likely that mergers and collaborative ventures tend to affect adversely to R&D competition Against this trend, enforcing agency of advanced countries including U.S.A are reassessing certain aspects of competition policy toward mergers and acquisition to ensure that procompetitive, efficiency-enhancing transactions are permitted. The role of competition policy is developing and appropriating new technology and protects the risks involved in the licensing contract of technologies. The role of intellectual property rights is also contrived to promote technological innovation and to increase consumer welfare. That is to say, dynamic efficiency of intellectual property rights includes (l) increase in social welfare and (2) promotion of growth by improvement of quality through invention and commercialization of new product as well as enhanced productive efficiency thorough appropriating new process. Because intellectual property rights are licensed to make use of complementary inputs, the rule of reason approach seems proper when applying antitrust law. To analyze the "Antitrust Guidelines for the Licensing and Acquisition of Intellectual Property"by DOJ and FTC in U.S.A, the author surveyed pros and cons on innovation market approach. This approach will only be used in a narrow range of situations when the evidence is solid, concentration numbers are extremely high, and the agencies can predict with a high degree of certainty that the merger will likely lead either to a slowing in the pace of innovation or the loss of an alternative research track that is likely to lead to a product beneficial to consumers. The author introduces the studies on licensing contract of intellectual property rights and competition polices on behalf of potential inquirers. Also the author invites the interdisciplinary researchers to analyze further with a model on the aspects of the "Notice 1995-10 for Types and Criteria on Unfair Transaction Behavior in International Contracts" by Fair Trade Committee of Korea.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.7 no.3
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• pp.1-9
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• 2017

Financial instruments are economic and intangible assets that bring financial company tremendous economic returns when it is a success. Also, It is necessary to protect this because it is a product of effort made by a lot of human resources and materials. However, legal and institutional devices for financial instruments are insufficient currently and 'copying practices' are rampant throughout the industry in korea. This ultimately inhibits the utility and welfare of consumers, but also adversely affects the competitiveness of the financial industry. In order to finance innovation that new financial products and services have appeared, it is necessary to grant the appropriate rights, such as intellectual property rights of financial instruments. And, there is a need for measures to protect it. Thus, this study proposed new way protecting the financial instruments through Intellectual property right. It is the introduction of similar protection system to financial instruments, such as mechanisms that protect database producers in copyright law.

• Interdisciplinary Bio Central
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• v.4 no.4
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• pp.11.1-11.8
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• 2012

Genes that are indispensable for survival are referred to as essential gene. Due to the momentous significance of these genes for cellular activity they can be selected potentially as drug targets. Here in this study, an essential gene for Streptococcus suis was predicted using coherent statistical analysis and powerful genome comparison computational method. At first the whole genome protein scatter plot was generated and subsequently, on the basis of statistical significance, a reference genome was chosen. The parameters set forth for selecting the reference genome was that the genome of the query (Streptococcus suis) and subject must fall in the same genus and yet they must vary to a good degree. Streptococcus pneumoniae was found to be suitable as the reference genome. A whole genome comparison was performed for the reference (Streptococcus pneumoniae) and the query genome (Streptococcus suis) and 14 conserved proteins from them were subjected to a screen for potential essential gene property. Among those 14 only one essential gene was found to be with impressive similarity score between reference and query. The essential gene encodes for a type of 'Clp protease'. Clp proteases play major roles in degrading misfolded proteins. Results found here should help formulating a drug against Strptococcus suis which is responsible for mild to severe clinical conditions in human. However, like many other computational studies, the study has to be validated furthermore through in vitro assays for concrete proof.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.15-19
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• 2006

A ferredoxin adsorbed hetero self-assembled layer was fabricated on chemically modified Au substrate, 4-Aminothiophenol (4-ATP) was deposited onto Au substrate and then N-succinimidyl-3-[2-pyridyldithio] propionate (SPDP) was adsorbed on the 4-ATP layer, since SPDP was used as a bridging molecule for ferredoxin adsorption, Ferredoxin/SPDP/4-ATP structured hetero layer was constructed because of strong chemical binding of ferredoxin, SPDP, and 4-ATP, The surface of the ferredoxin-adsorbed SPDP/4-ATP layer was observed by scanning tunneling microscopy, The hetero film formation was verified by surface plasmon resonance measurement. The current flow and rectifying property based on the scanning tunneling spectroscopy I-V characteristics was achieved in the proposed hetero layer. Thus, the hetero layer structure of ferredoxin functioned as a molecular diode with rectifying property, The proposed molecular diode can be usefully applied for the development of molecular scale electronic devices.

• Journal of the Society of Naval Architects of Korea
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• v.53 no.4
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• pp.258-265
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• 2016

A flexible riser consists of several layers which have different materials, shapes and functions. The layers designed properly can take the design load safely, and each property of layer provides a complexity of flexible riser. Such complexity/unit-property is an input for global analysis of flexible riser. There are several approaches to calculate the complexity of flexible riser, those are experimental, numerical and theoretical methods. This paper provides a complexity from numerical and theoretical analysis for 2.5 inch flexible riser of which details and the experimental data are already produced under tension, external pressure, and bending moment. In addition, comparison of stiffness and stress are also provided. Especially, analysis of stress could lead to researches on ultimate strength or fatigue strength of flexible risers.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.5
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• pp.555-559
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• 2011

Oil hydraulic piston pumps are extensively used worldwide because of their simple design, light weight, and cost effectiveness. However, an oil hydraulic pump is likely to have high leakage, friction, and low energy efficiency after long-term use. In oil hydraulic piston pumps the clearance between the valve block and the piston plays an important role in the volumetric and overall efficiency. We studied the wear property of the SACM645 material used in hydraulic piston pumps via experiments with different heat treatments. We prepared three different specimens. The maximum tensile strengths of the QT and QT-nitration specimens are similar (about 820 MPa), but the strains are significantly different. However, the fatigue characteristic depended on the heat treatment.

• Coupled systems mechanics
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• v.2 no.2
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• pp.147-157
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• 2013

We numerically investigate the electronic band structure of carbon nanotubes (CNTs) under radial corrugation. Hydrostatic pressure application to CNTs leads to a circumferential wave-like deformation of their initially circular cross-sections, called radial corrugations. Tight-binding calculation was performed to determine the band gap energy as a function of the amplitude of the radial corrugation. We found that the band gap increased with increasing radial corrugation amplitude; then, the gap started to decline at a critical amplitude and finally vanished. This non-monotonic gap variation indicated the metal-semiconductor-metal transition of CNTs with increasing corrugation amplitude. Our results provide a better insight into the structure-property relation of CNTs, thus advancing the CNT-based device development.

• Journal of Power System Engineering
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• v.21 no.6
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• pp.21-30
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• 2017

This study deals with the characteristics of degraded stainless steel. Stainless steel is heat treated to ensure mechanical properties when designing or manufacturing machinery parts or equipment. In this study, the mechanical properties and wear characteristics of three kinds of stainless steels after artificially heat-treated at 753 K~993 K, where chrome depletion occurs near the grain boundary, were evaluated. The microstructure and fracture surface were also observed. From the results, friction coefficient and wear loss decreased with increasing the heat treatment temperature regardless of the type of stainless steel. Also, as the tensile strength increased, the friction coefficient and wear loss decreased. Wear loss showed proportional to a tendency to increase with increasing friction coefficient.