• BMB Reports
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• 제37권1호
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• pp.45-52
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• 2004

The goal of interaction proteomics that studies the protein-protein interactions of all expressed proteins is to understand biological processes that are strictly regulated by these interactions. The availability of entire genome sequences of many organisms and high-throughput analysis tools has led scientists to study the entire proteome (Pandey and Mann, 2000). There are various high-throughput methods for detecting protein interactions such as yeast two-hybrid approach and mass spectrometry to produce vast amounts of data that can be utilized to decipher protein functions in complicated biological networks. In this review, we discuss recent developments in analytical methods for large-scale protein interactions and the future direction of interaction proteomics.

• 대한수학교육학회지:수학교육학연구
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• 제10권2호
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• pp.279-300
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• 2000

This research investigated students' interaction in the environment with dynamic geometry software such as Cabri II, and GSP in order to understand and analyze why computer environment is a richer interaction field for developing children's explorative ability than other traditional paper-and-pencil environments. This research focused on 5th graders' interaction with topics of transformational geometry and similar figure and analyzed children's learning process and their interview results gotten through audio and video recording. Computer exploration with a dynamic software seems to be very helpful for elementary students to learn geometry. However, the effectiveness of the computer should be discussed with respected to its methodological validity of teachers to guide students' explorative activities with a dynamic software.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.644-647
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• 2017

Peptidyl prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds preceding prolines. We investigated the protein-protein interaction between a 22 kDa PPIase (VaFKBP22, an FK506-binding protein) and the molecular chaperone DnaK derived from Vibrio anguillarum O1 (VaDnaK) using GST pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. Furthermore, we analyzed the three-dimensional structure of the protein-protein interaction. Based on our results, VaFKBP22 appears to act as a cochaperone of VaDnaK, and contributes to protein folding and stabilization via its peptidyl-prolyl cis/trans isomerization activity.

• Bulletin of the Korean Chemical Society
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• 제34권12호
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• pp.3591-3596
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• 2013

The enantioselective aza-Morita Baylis Hillman reaction of nitroalkene and N-tosylimine catalyzed by thiourea-tertiary amine has been investigated using density functional theory. Enantioselectivity is dominated by the cooperative effect of non-covalent and weak covalent interactions imposed by different units of catalyst. As Lewis base, the tertiary amine unit activates nitroalkene via weak covalent bond. The weak covalent interaction orients the reaction in a major path with smaller variations of this bond. The aromatic ring unit activates N-tosylimine via ${\pi}-{\pi}$ stacking. The non-covalent interaction selects the major path with smaller changes of the efficient packing areas. Thiourea unit donates more compact H-bonded network for species of the major path. The calculated ee value in xylene solution phase (97.6%) is much higher than that in N,N-Dimethylformamide (27.2%). Our conclusion is also supported by NBO analysis.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2006년 제4회 한국유체공학학술대회 논문집
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• pp.497-500
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• 2006

An theoretical analysis on the electro-osmotic flow in a 2-dimensional slit, that is induced by an external electric field acting on the electrical double layers near the slit wall, was performed. Especially, although there were many studies on the interacting electrical double layers, it was found in this study that they have several physical or mathematical fallacies. To resolve these, the general solution on the charge-regulating slit with the height as a parameter was obtained. The results of this work can provide the electrokinetic solution of nanoscale slit with the electrical double layer interaction as well as that of microscale slit without the interaction and can be used as the benchmark of a numerical analysis and the reference of electrokinetic flow path design.

• Journal of Photoscience
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• 제12권3호
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• pp.113-117
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• 2005

Spectroscopic investigations on the interaction of gabapentin (GBP) with bovine serum albumin (BSA) were reported. The association constant of GBP-BSA system was determined at different temperatures (298, 302, 306 and 311 K) based on the fluorescence quenching results. The GBP was found to quench the fluorescence of BSA through static mechanism. Thermodynamic parameters, the standard enthalpy change, $({\Delta}H^o)$ and the standard entropy change $({\Delta}S^o)$ were observed to be $-9.61{\pm}0.008\;kJ\;mol^{-1}$ and $3.58{\pm}0.011\;Jmol^{-1}K{-1}$ respectively. These indicated that the hydrophobic and electrostatic forces played a role in the interaction of GBP with BSA. The negative value of ${\Delta}G^o$ revealed that the binding reaction is spontaneous. The circular dichroism studies indicated the conformational changes in BSA upon interaction with GBP. The effect of some metal ions on the binding constant was also investigated.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2003년도 춘계학술대회논문집
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• pp.420-423
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• 2003

This paper addresses the results of experimental and analytical study on the effects of dynamic interaction between vehicle and bridge on modal properties of bridge. Based on ambient vibration test and vehicle impact test on a bridge, it is found that the natural frequencies of bridge are varied by vehicle passing. Analytical studies for the effects of vehicle position, speed, damping, mass ratio and frequency ratio on bridge-vehicle interaction are carried out using complex eigenvalue analysis and numerical integration in time domain. The results show that vehicle properties except speed cause significant change of natural frequency as well as damping of bridge.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 추계학술대회 논문집
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• pp.363-368
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• 2004

Maglev vehicles, which are levitated and propelled by electromagnets, often run on elevated guideways comprised of steel, aluminum and concrete. Therefore, an analysis of the dynamic interaction between the Maglev vehicle and the guideway is needed in the design of the critical speed, ride, controller design and weight reduction of the guideway. This study introduces a dynamic interaction simulation technique that applies FEM. The proposed method uses FEM to model the elevated guideway and the Maglev vehicle, which is different from conventional studies. Because the proposed method uses FEM, it is useful to calculate the deformation of the elevated guideway, the dynamic stress, and the motion of the vehicle. By applying the proposed method to an urban transit Maglev vehicle, UTM01, the dynamic response is simulated according to velocity increase and can be reviewed again. From the result of the study, we concluded that FEM simulation of the dynamic interaction between the maglev vehicle and the guideway is possible.

• 한국지진공학회논문집
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• 제21권5호
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• pp.227-236
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• 2017

This study reviews concepts, theories and formulas included in standards on soil-structure interaction and also shows practical example of application for engineers. Real structures are 3 dimensional and multi degree of freedom but they are often idealized to single degree of freedom for convenience. In this study, detailed procedures to calculate soil spring constants and damping coefficients and method to model soil-structure system are explained. Additionally, case studies to judge fixed base condition and evaluation of applicability of simple analysis method based on response spectra are performed.

• Bulletin of the Korean Chemical Society
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• 제34권1호
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• pp.231-236
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• 2013

Some cytotoxicity studies for the interpretation of the interaction between nanoparticles and cells are non-mechanistic and time-consuming. Therefore, non-biological screening methods, which are faster and simpler than in-vivo and in-vitro methods, are required as alternatives to current cytotoxicity tests. Here, we proposed a simple screening method for the analysis of the interaction between several AgNPs (bare-, citrate-, and polyvinylpyrrolidone-coating) and dye-containing vesicles acting as a biomimetic cell-membrane. The interaction between AgNPs and vesicles could be evaluated readily by UV-vis spectra. Absorbance deviation in UV-vis spectra revealed a large attraction between neighboring particles and vesicles. This was confirmed by (Derjagin, Landau, Verwey, and Overbeek) theory and DMF (dark-field microscopy) analysis. This proposed method might be useful for analyzing the cytotoxicity of nanoparticles with cell-membranes instead of in vitro or in vivo cytotoxicity tests.