• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.631-638
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• 2008

Tandem affinity purification (TAP) method combined with LC-MS/MS is the most accurate and reliable way to study the interaction of proteins or proteomics in a genome-wide scale. For the first time, we used a TAP-tag as a mutagenic tool to disrupt protein interactions at the specific site. Although lots of commonly used mutational tools exist to study functions of a gene, such as deletional mutations and site-directed mutagenesis, each method has its own demerit. To test the usefulness of a TAP-tag as a mutagenic tool, we applied a TAP-tag to RNA polymerase II, which is the key enzyme of gene expression and is controlled by hundreds of transcription factors even to transcribe a gene. Our experiment is based on the hypothesis that there will be interrupted interactions between Pol II and transcription factors owing to the TAP-tag attached at the C-terminus of each subunit of Pol II, and the abnormality caused by interrupted protein interactions can be observed by measuring a cell-cycle of each yeast strain. From ten different TAP-tagged strains, Rpb7- and Rpb12-TAP-tagged strains show severe defects in growth rate and morphology. Without a heterodimer of Rpb4/Rpb7, only the ten subunits Pol II can conduct transcription normally, and there is no previously known function of Rpb7. The observed defect of the Rpb7-TAP-tagged strain shows that Rpb7 forms a complex with other proteins or compounds and the interruption of the interaction can interfere with the normal cell cycle and morphology of the cell and nucleus. This is a novel attempt to use a TAP-tag as a proteomic tool to study protein interactions.

• Journal of the Korean Society for Precision Engineering
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• v.24 no.1 s.190
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• pp.101-109
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• 2007

In situations of spatio-temporal perception about visuo-auditory stimulus, researches propose optimal integration hypothesis that perceptual process is optimized to the interaction of the senses for the precision of perception. So, when the visual information considered generally dominant over any other sense is ambiguous, the information of the other sense like auditory stimulus influences the perceptual process in interaction with visual information. Thus, we performed two different experiments to certain the conditions of the interacting senses and influence of the condition. We consider the interaction of the visuo-auditory stimulation in the free space, the color of visual stimulus and sex difference of testee with normal people. In first experiment, 12 participants were asked to judge the change in the frequency of audio-visual stimulation using a visual flicker and auditory flutter stimulation in the free space. When auditory temporal cues were presented, the change in the frequency of the visual stimulation was associated with a perceived change in the frequency of the auditory stimulation as the results of the previous studies using headphone. In second experiment, 30 male and 30 female were asked to judge the change in the frequency of audio-visual stimulation using a color of visual flicker and auditory flutter stimulation. In the color condition using red and green. Both male and female testees showed same perceptual tendency. male and female testees showed same perceptual tendency however, in case of female, the standard deviation is larger than that of male. This results implies that audio-visual asymmetry effects are influenced by the cues of visual and auditory information, such as the orientation between auditory and visual stimulus, the color of visual stimulus.

• Journal of Korean Academy of Nursing
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• v.13 no.2
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• pp.1-21
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• 1983

The purposes of this study were to(a) develop theoretical modifications of the extended gate control theory of pain using Fishbein's model and（b) test the efficacy of these modifications. Attitude, social subjective norm, personal subjective norm, habit and state anxiety were operationalized to represent internal stimuli for the cognitive-evaluative and motivational-affective dimensions of the theory. Pain expression was operationalized as sensory and affective responses to pain, and pain endurance. Sixty-two female nurses from 20 to 50 years of age participated. A semantic differential scale measured attitude and motivations to comply; a Likerty-type scale measured personal and social norms and habit. Spielberger's STAI measured state anxiety, Pain was produced using a modified submaximum effort tourniquet technique. Pair expression was measured using ratio scales of sensory intensity and unpleasantness developed by Gracely and his associates. Pain endurance was measured by subtracting time of pain threshold from pain tolerance. The first hypothesis examining whether pain endurance would be more significantly related to the affective response than to the sensory response was net rejected. Four remaining hypotheses, testing the ability of the five variables to predict the sensory and affective responses were not rejected. However, the habit of pain expression and the attitude toward pain expression contributed to the prediction of both sensory and affective responses to pain. The interaction between the cognitive-evaluative and the sensory-discriminative dimensions and the interaction between the cognitive-evaluative and motivational-affective dimensions were partially supported by the data from these two variables. The interaction between the motivational-affective and the sensory-discriminative dimensions was also supported by the relationship of sensory to affective responses. The variables which did not significantly predict pain expression appeared to have potential for prediction. Revision and testing of the tools for better reliability, validity, and clinical usuability are needed. The study contributed to theory building. The identification of variables which pre-dict pain behavior must occur before effective nursing interventions can be developed.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.31 no.1
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• pp.17-27
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• 2019

We carry out the numerical simulation to test a hypothesis that freak waves can be triggered by the infragravity waves of bound mode underlying the ever-present swells and its constructive interaction with swells using the Tool Box called the ihFoam that has its roots on the OpenFoam, and Bi-spectrum. Numerical simulation is implemented for the SamChcuk LNG Plant where freak waves have been reported in front of the private wharf during its construction phase due to the uncompleted northern breakwater. Infra-gravity waves of bound mode is generated using the difference wave-wave interaction between the local wind waves of 7 s and a swell of 11.4 s based on the Bi-spectrum. For the sake of comparison, numerical simulation for infra-gravity waves of free mode is also carried out. Numerical results show that stem waves along the private wharf for SamChcuk LNG Plant can be triggered by the infra-gravity waves of bound mode coming from the north, which eventually leads to freak waves when encounters the reflected waves from the south jetty.

• International journal of steel structures
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• v.18 no.5
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• pp.1617-1630
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• 2018

The crucial differences between conventional rail with split-type connectors and continuous welded rails are axial stress in the longitudinal direction and stability, as well as other issues generated under the influence of loading effects. Longitudinal stresses generated in continuously welded rails on railway bridges are strongly influenced by the nonlinear behavior of the supporting system comprising sleepers and ballasts. Thus, the track structure interaction cannot be neglected. The rail-support system mentioned above has properties of non-uniform material distribution and uncertainty of construction quality. The linear elastic hypothesis therefore cannot correctly evaluate the stress distribution within the rails. The aim of this study is to apply the nonlinear finite element method using the nonlinear coupling interface between the track and structural model and to illustrate the welded rail behavior under the loading effect and uncertain factors of the ballast. Numerical results of nonlinear finite analysis with a three-dimensional solid and frame element model are presented for a typical track-bridge system. A composite plate girder, modeled by solid and shell elements, is also analyzed to consider the behavior of the welded rail. The analysis result showed buckling under the independent calculations of load cases, including 'temperature change', 'bending of the supporting structure', and 'braking' of the railway vehicle. A parametric study of the load combination method and the loading sequence is also included in this analysis.

• Communications for Statistical Applications and Methods
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• v.15 no.2
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• pp.265-271
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• 2008

It is commonly believed that diseases of human or economic traits of livestock are caused not by single genes acting alone, but multiple genes interacting with one another. This issue is difficult due to the limitations of parametric-statistic methods of gene effects. So we introduce multifactor-dimensionality reduction(MDR) as a methods for reducing the dimensionality of multilocus information. The MDR method is nonparametric (i. e., no hypothesis about the value of a statistical parameter is made), model free (i. e., it assumes no particular inheritance model) and is directly applicable to case-control studies. Application of the MDR method revealed the best model with an interaction effect between the SNPs, SNP1 and SNP3, while only one main effect of SNP1 was statistically significant for LMA (p < 0.01) under a general linear mixed model.

• Journal of Preventive Medicine and Public Health
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• v.15 no.1
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• pp.179-186
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• 1982

The methods controlling the confounding factors were discussed using the data of secondary infertility with induced abortion. Mantel-Haenszel method and logistic model were applied in the analysis to find out which factors were confounding and/or effect modification variables. In the logistic analysis, the main effect of induced abortion, spontaneous abortion, age and interaction effect between induced abortion and spontaneous abortion were chosen as independent variables being regressed into logistic functions. Spontaneons abortion was interpreted as a potential confounder and at the same time potential effect modifier and age was interpreted as potential confounder. Spontaneous abortion was shown to be more important influencing factor than age to the secondary infertility. In the course of logistic analysis, the problem of parameter estimation and hypothesis testing, assessing the fitness of a model, and selection of the best model were briefly explained. For the program of logistic model, FUNCAT Procedure of SAS package was chosen.

• Structural Engineering and Mechanics
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• v.33 no.5
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• pp.573-588
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• 2009

In this paper a four-node hybrid stress element is proposed for analysing arbitrarily shaped plates on a two parameter elastic foundation. The element is developed by combining a hybrid plate stress element and a soil element. The formulation is based on Hellinger-Reissner variational principle in which both inter element compatible boundary displacement and equilibrated stress fields for the plate as well as the foundation are chosen separately. This formulation also allows a low order polynomial interpolation functions. Numerical examples are presented to show that the validity and efficiency of the present element for the plate analysis resting on an elastic foundation. In these examples the effect of soil depth, interaction between closed plates on soil parameters, comparison with Winkler hypothesis is investigated.

• Korean Journal of Child Studies
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• v.25 no.5
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• pp.163-178
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• 2004

The hypothesis was tested that children whose families differ in socioeconomic status(SES) and educational level differ in their rates of productive language development because they have different language-learning experiences. Naturalistic interaction between mothers and their children was video taped. Transcripts of these interactions provided the basis for estimating the growth in children's productive vocabularies and properties of maternal speech. The sixty children from age 1 to 3 were selected in Yanji, China. The results show that the high educated mothers' children grew more than the low educated mothers' children in their mean length of utterances. Properties of maternal speech that differed as a function of mother's educational level fully accounted for this difference. Implications of these findings for mechanisms of environmental influence on child development are discussed.

• Journal of The Korean Astronomical Society
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• v.29 no.2
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• pp.83-91
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• 1996

We present a model that rotating primordial blackholes(PBHs) produced at the end of inflation generate the random, non-oriented primordial magnetic field. PBHs are copiously produced as the Universe completes the cosmic phase transition via bubble nucleation and tunneling processes in the extended inflation hypothesis. The PBHs produced acquire angular momentum through the mutual tidal gravitational interaction. For PBHs of mass less than 1013g, one can show that the evaporation (photon) luminosity of PBHs exceeds the Eddington limit. Thus throughout the lifetime of the rotating PBH, radiation flow from the central blackhole along the Kerr-geodesic exerts torque to ambient plasma. In the process similar to the Bierman's battery mechanism electron current reaching up to the horizon scale is induced. For PBHs of Grand Unified Theories extended inflation with the symmetry breaking temperature of $T_{GUT}\;\~\;10^{10}$ GeV, which evaporate near decoupling, we find that they generate random, non-oriented magnetic fields of $\~10^{-11}G$ on the last-scattering surface on (the present comoving) scales of $\~O(10)Mpc$.