• Journal of Korean Society on Water Environment
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• v.22 no.3
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• pp.437-443
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• 2006

The Water usage is relationship which is close with the administrative cost from industrial facility. It is not easy to reduce a water usage. This research is the optimization of the waste water quantity which process waste water integration of the standard thermal power plant in system operate time. The turbine rotates by force of the steam and it produces an electricity. Demineralization Water is manufacture purity manufacturing equipment and it is supplied in power plant channel. We knew a possibility of reducing from pure control process. When it is reduced the Back Washing time, Rinsing time of the gravity filter and the activated carbon filter. Also, It is possible even from regeneration phase in Condensate Polishing Demineralization System. In addition, There is also the water which the drain of the sampling water for watching the condition of power plant process will be able to use. Integrates these processes it will be able to reduce an annual 30,000 ton degree. The research is want to use the fundamental data for the water curtailment of the power plant.

• The Transactions of the Korean Institute of Power Electronics
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• v.27 no.5
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• pp.397-401
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• 2022

The construction of the ITER tokamak machine is ongoing at a 77% process rate to achieve the first plasma in 2025. The 18 sets of power supply systems comprising 400 MVA thyristor AC/DC converters for the superconducting magnets supplied by Korea (KO) are being installed with other systems, such as PF converters (China), DC busbars (Russia), and cooling water systems (India), in two buildings (Europe). The system interfaces have been defined during the design stage, and the systems have been manufactured. However, during the on-site installation work, several installation and integration issues emerged due to the manufacturing tolerance and design mistakes. To continue the installation and testing, the engineers of each system resolved the interface issues, planned the commissioning, and integrated the test plan. This paper describes the on-site installation status and issues and the commissioning plan of KO AC/DC converters.

• Spatial Information Research
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• v.20 no.6
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• pp.129-137
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• 2012

For construction project in extreme cold region, it is essential to establish basic data on the site such as topographical data from the early stage of construction of planning and designing, and it is needed to frequently perform site investigation when necessary. However, extreme cold regions are characteristic of being at long distance and difficult in approaching, and special regions such as Antarctica, in particular, are hard to conduct site investigation. Although a site investigation may be conducted, those who can visit Antarctica are sufficiently limited so that most of the staff may participate in construction without knowledge of the site and increase the risk of errors in decision making or designing. In order to resolve such problems, the authors in this study identified methods of building wide-area topographical data and bedrock classification data of exposed areas via remote sensing and of building precise topographical data on the construction site. Also, the authors attempted to present methods by which such data can be managed and visualized integrally via three-dimensional GIS technology and all the participants in construction can learn sense of field and conduct necessary analysis as frequent as possible. The areas around the Jangbogo Antarctic Station were selected to be the research area for conducting effective integrational management and three-dimensional visualization of various spatial data such as wide-area digital elevation model, ortho-images, bedrock classification data, local precise digital elevation model, and site images. The results of this study may enable construction firms to analyze local environments for construction whenever they need for construction in extreme cold regions and then support construction work including decision making or designing.

• Microbiology and Biotechnology Letters
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• v.29 no.1
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• pp.1-11
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• 2001

Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.1
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• pp.28-34
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• 2005

We review three methods of separating soil respiration into root and soil microbial contribution: (1) component integration, (2) root exclusion, and (3) isotopic method. Among these methods, component integration and root exclusion are most commonly used. Root respiration contribution to soil respiration estimated by the root exclusion method is higher than those by other two methods. Trenching has little environmental disturbances in soil or on surface of site compared to other methods in root exclusion such as root removal and gap formation. Isotopic method has an advantage over other methods because of minimal soil and root disturbances, but this method is costly and requires techniques for the complex analysis. Trenching seems to be an appropriate in situ method for calculating component contributions to soil respiration with minimum disturbances in site. However, the method overestimates the contribution of microbial respiration because of root decay, and realistic results could be obtained by estimating root decay or avoiding large roots in trenched plots.

• The Transactions of the Korea Information Processing Society
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• v.2 no.5
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• pp.645-654
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• 1995

A distributed DBMS integrates local schemas that are independently designed, maintained, and managed by different users at each site providing a global virtual schema. This global schema supports users at a specific site to transparently utilize local database at different sites. The security features of the local schema should also not be changed in the global schema integrating security features of each local schema. Researches on the integration of security features into local schema in distributed DBMS environment, however, are very rate. This pater using the multilevel secure object-oriented database model(as the model for the definition of a local schema in distributed environment) which is an extension of the object-oriented models. It also suggests eight integration methods that can maintain the security features of local schemas. The eight methods are classified by the object classes and by relationships among them.

• Journal of the Korean Association of Geographic Information Studies
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• v.23 no.4
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• pp.208-216
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• 2020

Recently, issues regarding underground safety such as sink hole, ground subsidence and damage to old underground facilities have been increasing in urban areas, raising the need for more accurate management of underground facilities. Thus, this study derived a technique for comparing spatial data of underground facilities acquired from GPR exploration results acquired at the site with spatial data of integrated underground spatial maps. Using this underground space integrated map-linked service prototype program developed through this study, comparing the location information of the GPR exploration results and the underground space integrated map for the verification of site usability in some sections around Gangnam Station, the results demonstrated that the location of the map is 0.879m maximum, minimum of 0.101m and the average fudge factor was 0.625m. If accuracy of the GPR exploration results is guaranteed, it is judged that it can be used to improve the location accuracy of the underground space integration map.

• Journal of Microbiology and Biotechnology
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• v.13 no.5
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• pp.783-788
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• 2003

A 27 bp $tRNA^{Gly}$ region (att1) was identified as the integration site for a 12,384 bp Pfl-derived genomic island containing 15 open reading frames (ORFs) from PA0715 to PA0729 in P. aeruginosa strain PAOl. Homologous island was observed in P. aeruginosa strain PA14, but not in P. aeruginosa strain K (PAK). We isolated the Pfl island from PA14, and determined its 10,657 bp sequences containing 14 ORFs, with significant sequence variations near the borders. In contrast to the PAO1 Pfl island, the PA14 Pfl island was integrated at the 10 bp att2 site between PA1191 and PA1192. The attl site of PA14, however, was still occupied by a third genetic segment, whereas both attl and att2 sites of PAK remained unutilized. These results exemplify an extensive genomic variation of Pfl-related islands involving differential genetic organizations and differential att site utilizations.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.4
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• pp.39-48
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• 2003

The Office of Korean National Railroads announced a design competition for Kwangyang Station and sought design proposals in October of 2002. The authors collaborated on a landscape design for Kwangyang Station and earned first prize in the competition. The Office decided to construct outdoor public spaces of Kwangyang Station for railroad users, visitors and staff, and local residents. The landscape design covers the following: site inventory and analysis; integration of design program and site analysis; evaluation of design concept; selecting a winning design alternative; schematic design including circulation form and spatial form; and design development including subarea design. In the design process, the authors focused on the integration and interpretation of site conditions as related to the Kwangyang Station building. They also collectively analyzed and considered the physical development of the outdoor spaces of Kwangyang Station. The following are some of the major areas in the design of the outdoor spaces of Kwangyang Station: 1) a plaza for waiting, 2) an entry area for passengers, 3) a train cafeteria, 4) a cultural plaza, 5) an image wall, and 6) a symbolic pond. The plaza for waiting is created as a place for users to wait and rest. The entry area for passengers provides an area for pedestrians approaching, passing and strolling. The train cafeteria features trains, lawns, grasses and trees. The cultural plaza is a main area representing the regional characteristics of Kwangyang. This plaza also provides an area for various public events. The image wall is meant to be an exhibition space for displaying the advancements of Korean National Railroads in sculptural image forms. The symbolic pond consists of an area with symbolic water features and several sculptures with artistic value. In the authors' design, the outdoor spaces of Kwangyang station are created as a new type of public realm with function, regional quality, original identity, cultural context, symbolic meaning and images, and beauty. The designed spaces would be one of the most representative places of interest and attraction in Kwangyang.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.35-39
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• 2001

The Integration Host factor (IHF) of Escherichia coli is a small, basic protein that is required for a variety of functions including site-specific recombination, transposition, gene regulation, plasmid replication, and DNA packaging. It ,is composed of two subunits that are encoded by the ihfA ($\alpha$-subunit) and ihjB ($\beta$-subunit) genes. IHF binding sites are composed of three elements called the WATCAR, TTG, and poly (dAT) elements. We have characterized IHF binding to the H site of bacteriophage λ. We have isolated suppressors that bind to altered H' sites using a challenge phage selection. Two different suppressors were isolated that changed the adjacent $\alpha$P64 and $\alpha$K65 residues. The suppressors recognized both the wild-type site and a site with a change in the WATCAR element. Three suppressors were isolated at $\beta$-E44. These suppressors bound the wild-type and a mutant site with a T：A to A：T change (H44A) in the middle of the TIR element. Site-directed mutagenesis was used to make several additional changes at $\beta$E44. The wild-type and $\beta$E44D mutant could not bind the wild-type site but were able to bind the H44A mutant site. Other mutants with neutral, polar, or a positive charge at $\beta$E44 were able to repress both the wild-type and H44A sites. Examination of the IHF crystal structure suggests that the ability of the wild-type and $\beta$E44D proteins to discriminate between the T：A and A：T basepairs is due to indirect interactions. The $\beta$-E44 residue does not contact the DNA directly. It imposes binding specificity indirectly by interactions with residues that contact the DNA. Details of the proposed interactions are discussed.